A5008600682- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Ion channel regulation and function
- Genetic Neurodegenerative Diseases
- Nerve injury and regeneration
- Genetics and Neurodevelopmental Disorders
- Pluripotent Stem Cells Research
- Caveolin-1 and cellular processes
- Adenosine and Purinergic Signaling
- Immune cells in cancer
- Neurological disorders and treatments
- Epilepsy research and treatment
- Pharmacological Effects and Toxicity Studies
- Photoreceptor and optogenetics research
- Cancer-related molecular mechanisms research
- Ion Channels and Receptors
- Lipid metabolism and disorders
- RNA regulation and disease
- Anesthesia and Neurotoxicity Research
- Immune Response and Inflammation
- Neurological Complications and Syndromes
- Neurological Disease Mechanisms and Treatments
- Cancer Research and Treatments
- Tuberous Sclerosis Complex Research
Shanghai Jiao Tong University
2015-2025
Songjiang District Central Hospital
2023-2024
Shanghai Children's Medical Center
2020-2024
Shanghai Center for Brain Science and Brain-Inspired Technology
2023
Shanghai Sixth People's Hospital
2023
First Affiliated Hospital of Anhui Medical University
2023
Anhui Medical University
2023
Shanghai First People's Hospital
2023
Center for Excellence in Molecular Cell Science
2021
Chinese Academy of Sciences
2009-2021
Intracellular Ca2+ transients are considered a primary signal by which astrocytes interact with neurons and blood vessels. With existing commonly used methods, has been studied only within astrocyte somata thick branches, leaving the distal fine branchlets endfeet that most proximate to neuronal synapses vessels largely unexplored. Here, using cytosolic membrane-tethered forms of genetically encoded indicators (GECIs; cyto-GCaMP3 Lck-GCaMP3), we report well-characterized approaches overcome...
The spatiotemporal activities of astrocyte Ca2+ signaling in mature neuronal circuits remain unclear. We used genetically encoded and glutamate indicators as well pharmacogenetic electrical control neurotransmitter release to explore activity the hippocampal mossy fiber pathway. Our data revealed numerous localized, spontaneous signals branches territories, but these were not driven by or glutamate. Moreover, evoked changed linearly with number action potentials. Under settings, responses...
Abstract NG2 glia, also known as oligodendrocyte precursor cells (OPCs), play an important role in proliferation and give rise to myelinating oligodendrocytes during early brain development. In contrast other glial cell types, the most intriguing aspect of glia is their ability directly sense synaptic inputs from neurons. However, whether this interaction bidirectional or unidirectional, its physiological relevance has not yet been clarified. Here, we report that form complexes with...
NG2 cells originate from various brain regions and migrate to their destinations during early development. These express voltage-gated Na+ channels but fail produce typical action potentials. The physiological role of in these is unclear. We found that GABA induces membrane depolarization Ca2+ elevation cells, a process requiring activation GABAA receptors, channels, Na+/Ca2+ exchangers (NCXs), not channels. have identified persistent current may underlie the GABA-induced pathway prolonged...
The discovery of intracellular Ca 2+ signals within astrocytes has changed our view how these ubiquitous cells contribute to brain function. Classically thought merely serve supportive functions, are increasingly respond to, and regulate, neurons. use organic indicator dyes such as Fluo-4 Fura-2 proved instrumental in the study astrocyte physiology. However, progress recently been accelerated by cytosolic membrane targeted genetically encoded calcium indicators (GECIs). Herein, we review...
Spinal cord injury (SCI) often leads to physical limitations, persistent pain, and major lifestyle shifts, enhancing the likelihood of prolonged psychological stress associated disorders such as anxiety depression. The mechanisms linking with regeneration remain elusive, despite understanding detrimental impact chronic on SCI recovery. In this study, we investigated effect primary sensory axon using a preconditioning lesions mouse model. Our data revealed that stress-induced mitochondrial...
The contribution of the inwardly rectifying K+ channel subtype Kir4.1 has been focused mainly on astrocytes, where they play important roles in maintenance resting membrane potential, extracellular uptake, and facilitation glutamate uptake central nervous system. Here, we report role channels NG2-glia during brain development, potassium signaling, an ischemic stroke disease model. are widely expressed development. In adult mouse hippocampus, constitute more than 80% inward currents. This...
Chronic hypoxia, common in neonates, disrupts gut microbiota balance, which is crucial for brain development. This study utilized cyanotic congenital heart disease (CCHD) patients and a neonatal hypoxic rat model to explore the association. Both rats CCHD infants exhibited immaturity, white matter injury (WMI), inflammation, motor/learning deficits. Through 16s rRNA sequencing metabolomic analysis, reduction
Summary Aims Nerve growth factor ( NGF ) has been reported to prevent neuronal damage and contributes the functional recovery in animal brain injury models human ischemic disease as well. We aimed investigate a potential therapeutic effect of gene treatment stroke estimate both at cellular cognitive levels an ischemia rat model. Methods After microinjection pseudolentivirus‐delivered β‐ into established model rats tMCAO ), we estimated cell apoptosis with TUNEL labeling neurogenesis by...
Oligodendrocytes have robust regenerative ability and are key players in remyelination during physiological pathophysiological states. However, the mechanisms of brain microenvironmental cue regulation differentiation oligodendrocytes still needs to be further investigated.We demonstrated that myelin-associated glycoprotein (MAG) was a novel receptor for angiopoietin-like protein 2 (ANGPTL2). The binding ANGPTL2 MAG efficiently promoted vitro, as evaluated an HCN cell line. Angptl2-null mice...
The role of GABA A receptor (GABA R)-mediated tonic inhibition in interneurons remains unclear and may vary among subgroups. Somatostatin (SOM) the hilus dentate gyrus show negligible expression nonsynaptic R subunits very low inhibition. To determine effects ectopic subtypes these neurons, Cre-dependent viral vectors were used to express GFP-tagged (α6 δ) selectively hilar SOM neurons SOM-Cre mice. In single-transfected animals, immunohistochemistry demonstrated strong either α6 or δ...
Abstract Pericytes are one of the main components neurovascular unit. They play a critical role in regulating blood flow, blood–brain barrier permeability, neuroinflammation, and neuronal activity. In central nervous system (CNS), pericytes classified into three subtypes, that is, ensheathing, mesh, thin‐strand pericytes, based on their distinct morphologies region‐specific distributions. However, whether these types exhibit heterogeneity or homogeneity with regard to membrane properties has...