A5055340399- Asthma and respiratory diseases
- IL-33, ST2, and ILC Pathways
- Pediatric health and respiratory diseases
- Eosinophilic Esophagitis
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Immune Cell Function and Interaction
- Inhalation and Respiratory Drug Delivery
- Neonatal Respiratory Health Research
- Multiple Myeloma Research and Treatments
- Mast cells and histamine
- Cystic Fibrosis Research Advances
- Cytomegalovirus and herpesvirus research
- Immunotherapy and Immune Responses
- Respiratory and Cough-Related Research
- Toxoplasma gondii Research Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Genetic Mapping and Diversity in Plants and Animals
- Phagocytosis and Immune Regulation
- Protein Degradation and Inhibitors
- HIV/AIDS drug development and treatment
- Bacterial biofilms and quorum sensing
- Herpesvirus Infections and Treatments
- Antimicrobial Peptides and Activities
- Pneumonia and Respiratory Infections
- Transgenic Plants and Applications
Cancer Center Amsterdam
2019-2023
Vrije Universiteit Amsterdam
2021-2023
Amsterdam University Medical Centers
2019-2023
Lund University
2012-2022
Institute of Infection and Immunity
2019
University of Amsterdam
2017-2019
Amsterdam UMC Location University of Amsterdam
2016-2017
Skåne University Hospital
2014
Respiratory Clinical Trials
2014
Universitat Pompeu Fabra
2012
Abstract Innate lymphoid cells (ILCs) are crucial for the immune surveillance at mucosal sites. ILCs coordinate early eradication of pathogens and contribute to tissue healing remodeling, features that dysfunctional in patients with cystic fibrosis (CF). The mechanisms by which CF-immunopathology ill-defined. Here, we show group 2 (ILC2s) transdifferentiated into IL-17-secreting presence epithelial-derived cytokines IL-1β, IL-23 TGF-β. This conversion is abrogated IL-4 or vitamin D3. IL-17...
Highlights•NRP1+ ILC3s are present in lymphoid tissues, but not the peripheral blood or skin•NRP1+ express CD45RO and produce higher amounts of cytokines than NRP1− ILC3s•NRP1 is a marker for human with LTi phenotype vitro activity•NRP1+ lung tissues from smokers COPD patientsSummaryHere, we characterize subset that Neuropilin1 (NRP1) skin. NRP1+ group 3 innate cells (ILC3s) display tissue inducer (LTi) activity. In agreement this, mainly located proximity to high endothelial venules (HEVs)...
The CD38-targeting antibody daratumumab has marked activity in multiple myeloma (MM). Natural killer (NK) cells play an important role during therapy by mediating antibody-dependent cellular cytotoxicity via their FcγRIII receptor (CD16), but they are also rapidly decreased following initiation of treatment. We characterized the NK cell phenotype at baseline and monotherapy flow cytometry time flight to assess its impact on response development resistance (DARA-ATRA study; NCT02751255). At...
Summary Background The toll‐like receptors, TLR5 and TLR7, have recently been proposed in asthma immunopathogenesis. While supporting data come from animal or vitro studies, little is known about TLR7 expression human asthmatic airways. Methods Advanced immunohistochemical mapping of was performed on bronchial transbronchial biopsies healthy individuals patients with moderate severe asthma. Results identified multiple structural cells; epithelium, alveolar type II pneumocytes, plasma cells,...
<h3>Rationale</h3> The alveolar pathology in chronic obstructive pulmonary disease (COPD) involves antigen-driven immune events. However, the induction sites of adaptive responses have remained poorly investigated. <h3>Objectives</h3> To explore hypothesis that interfaces between lumen and lymphoid aggregates (LAs) provide a structural basis for increased antigen uptake COPD lungs. <h3>Methods</h3> Lung samples from patients with mild (Global Initiative Chronic Obstructive Disease (GOLD)...
Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is often associated with chemotherapy- and radiotherapy-induced host tissue damage, leading to graft-versus-host disease (GVHD). Innate lymphoid cells (ILC) have an essential role in homeostasis repair via their production of interleukin (IL)-22, which acts on intestinal cells. The healing capacities ILC IL-22 the context allo-HSCT GVHD has previously been demonstrated a mouse model for acute GVHD. We investigated potential other...
Infections in cystic fibrosis (CF), often involving Pseudomonas aeruginosa, result from a dysregulated airway immunity where one hallmark is the accumulation of necrotic and apoptotic immune cells, particular neutrophils. In addition, neutrophils actively release DNA, forming neutrophil extracellular traps (NETs) that contain antimicrobial proteins. Altogether, free DNA complex with actin accumulates lumen, resulting highly viscous sputum provides an anionic matrix, binding cationic this...
We have investigated the previously uncharacterized human cytomegalovirus (HCMV) UL1 open reading frame (ORF), a member of rapidly evolving HCMV RL11 family. is specific; absence in chimpanzee (CCMV) and sequence analysis studies suggest that may originated by duplication an ancestor gene from RL11-TRL cluster (TRL11, TRL12, TRL13). Sequence similarity searches against immunoglobulin (Ig)-containing proteins revealed pUL1 shows significant to cellular carcinoembryonic antigen-related (CEA)...
Proteochemometrics is a technology for the study of molecular recognition based on chemometric techniques. Here we applied it to analyse amino acids and acid physico-chemical properties that are involved in antibodies' peptide antigens. To this end, used system comprised by diverse single chain antibody library derived from murine mAb anti-p24 (HIV-1) CB4-1, evaluated arrays manufactured SPOT synthesis. The binding pattern obtained was correlated descriptors (z-scales) antibodies peptides...
In cystic fibrosis (CF), colonization of the airways with <i>Pseudomonas aeruginosa </i>is associated disease deterioration. The mechanism behind progression is not fully understood. present work shows that antibacterial chemokine MIG/CXCL9 in and sputum CF patients. showed high bactericidal activity against. <i>P. aeruginosa, </i>including some strains from Full-length was detected healthy controls patients colonized aeruginosa. </i>However, degraded only found...
Viral infections predispose to the development of childhood asthma, a disease associated with increased lung mast cells (MCs). This study investigated whether viral lower respiratory tract (LRTIs) can already evoke MC response during childhood. Lung tissue from young children who died following LRTIs were processed for immunohistochemical identification MCs. Children nonrespiratory causes served as controls. MCs examined in relation sensitisation infant mice exposed allergen influenza A...
Contrasting the antigen-presenting dendritic cells (DCs) in conducting airways, alveolar DC populations human lungs have remained poorly investigated. Consequently, little is known about how DCs are altered diseases such as chronic obstructive pulmonary disease (COPD). This study maps multiple tissue categories distal lung across COPD severities. Specifically, single-multiplex immunohistochemistry was applied to quantify langerin/CD207+, CD1a+, BDCA2+, and CD11c+ subsets compartments from...
Abstract Toll-like receptors are a major class of pattern and damage recognition controlling innate immune responses. TLR5 recognizes bacterial flagellin TLR7 identifies single stranded viral RNA. The present study characterizes expression in central airways asthmatics. Methods: Bronchial biopsies were obtained from healthy controls, uncontrolled asthmatics on inhaled glucocorticoids, severe oral glucocorticoids. Subjects classified according to GINA guidelines asthma control score. Tissue...
A subset of patients with severe asthma (SA) remains persistently symptomatic despite treatment inhaled and oral administration corticosteroids. The aim present study was to map the pattern infiltrating leukocytes structural parameters in bronchi this type asthma. Bronchial biopsies were obtained from 25 SA that classified as either stable (n=15) or (n=10). Biopsies 8 healthy subjects served control material. Tissue sections stained an automated immunohistochemistry robot subsequently...