Several studies have suggested that long intergenic noncoding RNAs are involved in the progression of diabetic nephropathy (DN). However, exact role and regulatory mechanism RNA (lncRNA) NR_038323 (DN) remain largely unclear. In present study, we found lncRNA overexpression ameliorated high glucose (HG)-induced expression levels collagen I, IV, fibronectin, whereas knockdown exerted opposite effects. Moreover, results bioinformatic prediction, luciferase assay, fluorescence situ...

Abstract Background To explore the mechanism of LINC00470 in serum exosomes from glioma patients regulating autophagy and proliferation cells. Methods Exosomes were extracted (GBM-exo). Expression was analyzed with clinicopathological characteristics patients. Glioma mouse model established. The effects LINC00470, miR-580-3p WEE1 on cell proliferation, as well activation PI3K/AKT/mTOR pathway measured. Dual luciferase reporter assay RNA immunoprecipitation (RIP) conducted to validate binding...

Recent studies have shown that autophagy exhibits a renoprotective role in various models of acute kidney injury (AKI). However, its vancomycin (Van)-induced AKI remains largely unclarified. This study was the first to indicate rapidly activated both human kidney-2 cells and renal tissues, mammalian target rapamycin (mTOR) inactivated via suppression ERK1/2 mTOR during Van treatment. Interestingly, for vitro vivo experiments, chloroquine PT-Atg7-KO significantly ameliorated Van-induced...

Epilepsy affects over 70 million people globally, with temporal lobe epilepsy hippocampal sclerosis (TLE-HS) often progressing to a drug-resistant state. Recent research has highlighted the role of reactive astrocytes and glutamate dysregulation in pathophysiology. This study aims investigate involvement astrocytic xCT, glutamate-cystine antiporter, its regulation by m6A reader protein YTHDC2 TLE-HS.

This study aimed to investigate the molecular mechanism underlying fibrosis in hypertrophic cardiomyopathy (HCM).Quantitative real-time polymerase chain reaction (qRT-PCR) was performed measure expression of potentially relevant microRNAs (miRNAs) and long noncoding RNAs (lncRNAs) patients with HCM suffering from free fibrosis. In addition, regulatory relationship between lncRNAs miR-29a studied using a luciferase assay. Subsequently, area under receiver-operating characteristics (ROC) curve...

Abstract Breast cancer brain metastases (BCBM) is a devastating disease with dismal prognosis. Although chemotherapy widely used for clinical management of most tumors, it often ineffective BCBM. Therefore, alternative approaches improved treatment BCBM are in great demand. Here, an innovative gene therapy regimen reported that designed effective First, poly(lactone‐ co ‐β‐amino ester) nanoparticles capable efficient delivery synthesized and engineered targeted to through surface conjugation...

MicroRNAs (miRs), a class of noncoding RNAs that are 18‐25 nucleotides in length, able to suppress gene expression by targeting complementary regions mRNAs and inhibiting protein translation. Recently, miR-181b was found play suppressive role glioma, but the regulatory mechanism malignant phenotypes glioma cells remains largely unclear. In this study, we significantly downregulated tissues when compared with normal brain tissues, decreased levels were associated high-grade pathology poor...

MicroRNAs (miRs) are a class of small non-coding RNAs 18-25 nucleotides in length that serve as key regulators the development and progression human cancers. Recently, miR‑30b‑5p, member miR‑30 family, has been reported to act tumor suppressor gastric cancer. However, expression function miR‑30a‑5p cancer, well corresponding underlying mechanism, remain unknown. In present study, it was demonstrated significantly reduced cancer tissues (n=43) compared with normal (n=10; P<0.01). Similarly,...

Intracranial aneurysm (IA) rupture is life-threatening. However, the mechanisms underlying IA initiation, progression, and remain poorly understood. In present study, we examined role of primary cilia in development.IA was experimentally induced mice with elastase angiotensin II treatment. The number cells determined both peri-IA regions. development assessed through knocking out or manipulating expression important components cilia. Finally human patients studied. model IA, significantly...

Endothelin-1 (ET-1)/endothelin A receptor (ETAR) signaling is important for osteosarcoma (OS) progression. Monoclonal antibodies (mAbs) targeting ganglioside GD2 reportedly inhibit tumor cell viability independent of the immune system. recent study suggests that may play an role in OS In present study, we first time explored effects anti-GD2 mAb alone or combination with ETAR antagonist on invasiveness and viability. Human lines Saos-2, MG-63 SJSA-1 were treated control IgG (PK136 mAb, 50...

MicroRNAs (miRNAs or miRs), a class of non-coding RNAs 18-25 nucleotides in length, act as key regulators the development and malignant progression various human cancers by modulating expression their target genes. Recently, miR‑16 has been demonstrated to be play role glioma. However, regulatory mechanisms glioma growth metastasis remain largely unclear. In present study, qRT-PCR revealed that was significantly downregulated 23 tissue specimens compared 7 normal brain specimens. Moreover,...

Epilepsy is a common neurological disease that can induce severe physiological brain damage, including nerve cell apoptosis. MicroRNAs (miRs) have been widely investigated in epilepsy therapy. miR‑135a‑5p expression levels children with temporal lobe were found to be significantly increased. However, whether participates epilepsy‑induced apoptosis not completely understood. In the present study, an <em>in vitro</em> model of BV2 microglia cells was induced using 6‑µm kainic acid (KA)....

Heparin-binding epidermal growth factor EGF-like (HB-EGF) is a member of the family that expressed in many cell types. We have previously reported effects HB-EGF on intestinal epithelial cells and endothelial after exposure to ischemia/reperfusion vivo or anoxia/reoxygenation injury vitro. However, effect neuronal largely unexplored. In this study, we examined neurite outgrowth pheochromocytoma (PC12) as well neuroprotective injured PC12 exposed oxygen glucose deprivation (OGD), which mimics...

ABSTRACT It has been demonstrated that B cells were decreased in the BM of SPARC-null mice, accompanied by a lack immune response to LPS. However, effect SPARC deficiency on lymphopoiesis remains unclear. Herein, we investigated role regulation lymphopoiesis, as well underlying molecular mechanisms. In present study, found size B-lineage progenitors (pro-B and pre-B plus immature cells) primitive hematopoietic (LSK LTC reduced, whereas multipotent (CFU-S12) increased mice. When transplanted...

In the present study, association between angiopoietin-like 4 (ANGPTL4) and aldolase A (ALDOA) in human melanoma cell invasion survival was investigated. Overexpression knockdown of ANGPTL4 were respectively performed WM-115 WM-266-4 cells. ALDOA expression at both mRNA protein levels as well gene promoter activities increased decreased parallel with overexpression cells, which blocked by selective kinase C (PKC) inhibitor restored PKC agonist, respectively. significantly matrix...

Abstract Apoptosis of vascular endothelial cells (VECs) is highly important in the occurrence and development atherosclerosis (AS). HomeboxC6 (HOXC6) expressed higher levels multiple malignant tissues, it influences biological behavior cancer cells. However, effects HOXC6 on AS apoptosis VECs have not been fully elucidated. In this study, we demonstrated that expression was increased aortic wall rats peripheral blood monocytes patients with coronary heart disease. Furthermore, uncovered BAX...

Crop molecular breeding primarily focuses on increasing the trait of plant yield. An elongator-associated protein, KTI12, is closely associated with biomass and KTI12 involved in developmental processes most organs, including leaf, root, flower, seed, through regulating cell division differentiation. Previous work has shown that upland cotton (Gossypium hirsutum), GhKTI12 regulates height, flowering, tolerance to salt drought stress. However, little known about regulation mechanism...

Abstract Background Osteosarcoma (OS) is the most common childhood bone cancer. Chemoresistance principal reason for poor survival and disease recurrence in OS patients, ET‐1 reportedly plays an important role development of chemoresistance cells. In present study, we first time explored association endothelin‐1 (ET‐1) SNPs haplotypes with risk chemoresistant pediatric OS. Procedure We genotyped three (rs1800541, rs2070699, rs5370) gene a case–control using 350 pairs age, sex, tumor location...

Catalase is an enzyme that widely occurs in animals and plants. It primarily used to decompose H2O2 the body of prevent oxidative damage biological macromolecules. The present study investigated influence fat levels feeding condition on gene expression catalase (CAT) Genetically Improved Farmed Tilapia (GIFT) tilapia (Oreochromis niloticus). A full-length 3063-bp cDNA sequence, encoding 527 amino acid residues CAT, was cloned, altered assayed by real-time polymerase chain reaction. sequence...