A5045810457- Photosynthetic Processes and Mechanisms
- Lipid metabolism and biosynthesis
- Algal biology and biofuel production
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Plant biochemistry and biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- Biocrusts and Microbial Ecology
- Glycosylation and Glycoproteins Research
- Hydrocarbon exploration and reservoir analysis
- Enzyme Catalysis and Immobilization
- Plant Surface Properties and Treatments
- Plant responses to water stress
- Antibiotic Resistance in Bacteria
- Nitrogen and Sulfur Effects on Brassica
- Enterobacteriaceae and Cronobacter Research
- Methane Hydrates and Related Phenomena
- Plant Physiology and Cultivation Studies
- Marine and coastal ecosystems
- Marine and coastal plant biology
- Advanced Thermodynamics and Statistical Mechanics
- Paleontology and Stratigraphy of Fossils
- Ion Transport and Channel Regulation
Tokyo Institute of Technology
2015-2024
National Institute of Advanced Industrial Science and Technology
2011
Japan Agency for Marine-Earth Science and Technology
2009
Michigan State University
2003-2005
Kirin (Japan)
1997
Shizuoka University
1997
The colonization of land by plants was a key event in the evolution life. Here we report draft genome sequence filamentous terrestrial alga Klebsormidium flaccidum (Division Charophyta, Order Klebsormidiales) to elucidate early transition step from aquatic algae plants. Comparison with that other and demonstrate K. acquired many genes specific We indeed produces several plant hormones homologues some signalling intermediates required for hormone actions higher also encodes primitive system...
Phosphate is an essential nutrient for plant viability. It well-established that phosphate starvation triggers membrane lipid remodeling, a process converts significant portion of phospholipids to non-phosphorus-containing galactolipids. This remodeling mediated by either phospholipase C (PLC) or D (PLD) in combination with phosphatidate phosphatase (PAP). Two PLC genes, NPC4 and NPC5, PLD PLDzeta1 PLDzeta2, are shown be involved the remodeling. However, gene knockout studies show none them...
Abstract Plants synthesize a sulfur-containing lipid, sulfoquinovosyldiacylglycerol, which is one of three nonphosphorus glycerolipids that provide the bulk structural lipids in photosynthetic membranes. Here, identification novel gene, UDP-glucose pyrophosphorylase3 (UGP3), required for sulfolipid biosynthesis described. Transcriptome coexpression analysis demonstrated highly correlated expression UGP3 with known genes Arabidopsis thaliana. Liquid chromatography–mass spectrometry leaf two...
When cultivated under stress conditions, many plants and algae accumulate oil. The unicellular green microalga Chlamydomonas reinhardtii accumulates neutral lipids (triacylglycerols; TAGs) during nutrient conditions. Temporal changes in TAG levels nitrogen (N)- phosphorus (P)-starved cells were examined to compare the effects of depletion on accumulation C. reinhardtii. fatty acid composition substantially changed depending cultivation stage before starvation. Profiles also differed between...
Diacylglyceryl-N,N,N-trimethylhomo-Ser (DGTS) is a nonphosphorous, polar glycerolipid that regarded as analogous to the phosphatidylcholine in bacteria, fungi, algae, and basal land plants. In some species of including stramenopile microalga Nannochloropsis oceanica, DGTS contains an abundance eicosapentaenoic acid (EPA), which relatively scarce phosphatidylcholine, implying has unique physiological role. this study, we addressed role N. oceanica We identified two biosynthetic enzymes have...
Monogalactosyldiacylglycerol (MGDG) synthase (UDPgalactose:1,2-diacylglycerol 3-β- d -galactosyltransferase; EC 2.4.1.46 ) catalyzes formation of MGDG, a major structural lipid chloroplast. We cloned cDNA for the from cucumber library. The full-length clone was 2142 bp, and it contains 1575-bp open reading frame encoding 525 aa. consists regions mature protein (422 aa; M r 46,552) transit peptide to chloroplast (103 aa). Although molecular weight region matched that purified cotyledons,...
Floods impede gas (O2 and CO2 ) exchange between plants the environment. A mechanism to enhance plant under water comprises films on hydrophobic leaves, but genetic regulation of this is unknown. We used a rice mutant (dripping wet leaf 7, drp7) which does not retain its wild-type (Kinmaze), in gene discovery for trait. Gene complementation was tested transgenic lines. Functional properties leaves as related film retention underwater photosynthesis were evaluated. Leaf Gas Film 1 (LGF1)...
MGDG synthase, the enzyme that catalyzes synthesis of major chloroplast membrane lipid monogalactosyldiacylglycerol (MGDG), is encoded by a multigenic family. We have analyzed biochemical properties, subcellular localization and topology spinach representative member type A family from Spinacia oleracea (soMGD A), using recombinant protein was functionally overexpressed in Escherichia coli specific polyclonal antibodies. demonstrated soMGD could catalyze both 'prokaryotic' 'eukaryotic'...
Microalgae accumulate triacylglycerols (TAGs) under conditions of nutrient stress. Phosphorus (P) starvation induces the accumulation TAGs, and cells P maintain growth through photosynthesis. We recently reported that starvation-dependent overexpression type-2 diacylglycerol acyl-CoA acyltransferase (CrDGTT4) from Chlamydomonas reinhardtii using a sulfoquinovosyldiacylglycerol synthase 2 (SQD2) promoter, which has increased activity during starvation, enhances TAG in C. cells. As result,...
Most microalgae abundantly accumulate lipid droplets (LDs) containing triacylglycerols (TAGs) under several stress conditions, but the underlying molecular mechanism of this accumulation remains unclear. In a recent study, we found that inhibition TOR (target rapamycin), highly conserved protein kinase eukaryotes, by rapamycin resulted in TAG microalgae, indicating negatively regulates accumulation. Here, show formation intracellular LDs and were also induced unicellular green alga...
Klebsormidium flaccidum is a charophytic alga living in terrestrial and semiaquatic environments. K. grows various habitats, such as low-temperature areas under desiccated conditions, because of its ability to tolerate harsh Wax cuticle polymers that contribute the layer plants are important for survival land plants, they protect against those environmental conditions were probably critical transition from aquatic microorganism plants. Bryophytes, non-vascular have similar, but simpler,...
The elucidation of lipid metabolism in microalgae has attracted broad interest, as their storage lipid, triacylglycerol (TAG), can be readily converted into biofuel via transesterification. TAG accumulates the form oil droplets, especially when cells undergo nutrient deprivation, such for nitrogen (N), phosphorus (P), or sulfur (S). biosynthesis under N-deprivation been comprehensively studied model microalga Chlamydomonas reinhardtii, during which dramatically. However, resulting rapid...
Acinetobacter baumannii presents a clinical challenge when it is non-susceptible to carbapenems. The prevalence of carbapenem-non-susceptible A. in Japan unclear, as previous studies have been limited scope. We investigated the spread and performed comparison with findings from overseas. A total 305 non-duplicate isolates spp. 176 medical facilities all geographical regions were tested for susceptibility antimicrobial agents by agar dilution method. Isolates MICs imipenem ≥4 mg/L underwent...
Extended-spectrum β-lactamases (ESBLs) have become a problem among AmpC-producing Enterobacteriaceae and the emergence of concomitant quinolone resistance in β-lactamase-producing isolates poses global threat. In this study we investigated prevalence regional variation ESBLs Japanese clinical Citrobacter spp. analysed plasmid-mediated (PMQR) determinants ESBL-producing spp.A total 348 collected throughout Japan were studied. Screening boronic acid disc test performed to detect with...
The current research investigated the regulation of monogalactosyldiacylglycerol (MGDG) biosynthesis, catalyzed by MGDG synthase (MGD) (UDP-galactose:1,2-diacylglycerol 3-β-d-galactosyltransferase; EC 2.4.1.46), during chloroplast development in cucumbers (Cucumis sativus L. cv. Aonagajibai). In etiolated seedlings, white light induced a transient increase MGD mRNA, followed subsequent enzyme activity. MGDG, digalactosyldiacylglycerol (DGDG), and linolenic acid (18 : 3) both DGDG accumulated...
The photosynthetic membranes of cyanobacteria and chloroplasts higher plants have remarkably similar lipid compositions. In particular, thylakoid both are composed galactolipids, which monogalactosyldiacylglycerol (MGDG) is the most abundant, although MGDG biosynthetic pathways different in these organisms. Comprehensive phylogenetic analysis revealed that synthase (MGD) homologs filamentous anoxygenic phototrophs Chloroflexi a close relationship with MGDs Viridiplantae (green algae land...
Phosphate (Pi) limitation causes drastic lipid remodeling in plant membranes. Glycolipids substitute for the phospholipids that are degraded, thereby supplying Pi needed essential biological processes. Two major types of membrane lipids occur higher plants: whereas one involves an increase concentration sulfoquinovosyldiacylglycerol plastids to compensate a decreased phosphatidylglycerol, other digalactosyldiacylglycerol (DGDG) synthesis and export DGDG extraplastidial membranes reduced...
Monogalactosyldiacylglycerol (MGDG), the most abundant lipid in thylakoid membranes, is involved photosynthesis and chloroplast development. MGDG lipase has an important role remodeling Chlamydomonas reinhardtii. However, process related to turnover of lysogalactolipid that results from degradation, monogalactosylmonoacylglycerol (MGMG), remains be clarified. Here we identified a homolog Arabidopsis thaliana lysophosphatidylcholine acyltransferase (LPCAT) characterized two independent...