A5031319910- Sarcoma Diagnosis and Treatment
- RNA Research and Splicing
- Congenital heart defects research
- IL-33, ST2, and ILC Pathways
- Immune cells in cancer
- RNA modifications and cancer
- Autism Spectrum Disorder Research
- Immune Response and Inflammation
- Uterine Myomas and Treatments
- Cancer-related molecular mechanisms research
- Genetics and Neurodevelopmental Disorders
- Pluripotent Stem Cells Research
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Adipose Tissue and Metabolism
- MicroRNA in disease regulation
- Genomics and Chromatin Dynamics
- Neurogenesis and neuroplasticity mechanisms
- Ovarian cancer diagnosis and treatment
- Lung Cancer Treatments and Mutations
- CRISPR and Genetic Engineering
- Neonatal Respiratory Health Research
- Genetic Syndromes and Imprinting
- Tissue Engineering and Regenerative Medicine
- Endometrial and Cervical Cancer Treatments
Nanjing Medical University
2018-2025
Changzhou No.2 People's Hospital
2024
Fuzhou Maternity and Child Health Care Hospital
2024
Tianjin Normal University
2023
Division of Undergraduate Education
2022
Framework
2022
Fujian Medical University
2020
Fujian Normal University
2018
Albert Einstein College of Medicine
2011-2017
University of Rochester
2014-2016
Objective— Long noncoding RNAs (lncRNAs) represent a rapidly growing class of RNA genes with functions related primarily to transcriptional and post-transcriptional control gene expression. There is paucity information about lncRNA expression function in human vascular cells. Thus, we set out identify novel smooth muscle cells gain insight into their role the cell phenotypes. Approach Results— sequencing (RNA-seq) coronary artery revealed 31 unannotated lncRNAs, including cell–enriched...
Genome-wide expression analysis using next generation sequencing (RNA-Seq) provides an opportunity for in-depth molecular profiling of fundamental biological processes, such as cellular differentiation and malignant transformation. Differentiating human neurons derived from induced pluripotent stem cells (iPSCs) provide ideal system RNA-Seq since defective neurogenesis caused by abnormalities in transcription factors, DNA methylation, chromatin modifiers lie at the heart some...
Hair follicles (HFs) undergo cyclical periods of growth, which are fueled by stem cells (SCs) at the base resting follicle. HF-SC formation occurs during HF development and requires transcription factor SOX9. Whether how SOX9 functions in maintenance remain unknown. By conditionally targeting Sox9 adult HF-SCs, we show that is essential for maintaining them. SOX9-deficient HF-SCs still transition from quiescence to proliferation launch subsequent hair cycle. However, once activated, bulge...
Disruptive mutation in the CHD8 gene is one of top genetic risk factors autism spectrum disorders (ASDs). Previous analyses genome-wide occupancy and reduced expression by shRNA knockdown committed neural cells showed that regulates multiple cell processes critical for functions, its targets are enriched with ASD-associated genes.To further understand molecular links between functions ASD, we have applied CRISPR/Cas9 technology to knockout copy induced pluripotent stem (iPSCs) better mimic...
Mammalian aging is a highly complex process, full mechanistic understanding of which still lacking. One way to help understand the molecular changes underlying through comprehensive analysis transcriptome, primary determinant age-related phenotypic diversity. Previous studies have relied on microarray examine gene expression profiles in different tissues organisms. However, shown microarray-based transcriptional profiling less accurate and not fully capable capturing certain intricacies...
Individuals with 22q11.2 Deletion Syndrome (22q11.2 DS) are a specific high-risk group for developing schizophrenia (SZ), schizoaffective disorder (SAD) and autism spectrum disorders (ASD). Several genes in the deleted region have been implicated development of SZ, e.g., PRODH DGCR8. However, mechanistic connection between these neuropsychiatric phenotype remains unclear. To elucidate molecular consequences deletion early neural development, we carried out RNA-seq analysis to investigate...
Long noncoding RNAs (lncRNA) represent a growing class of genes with diverse cellular functions. We previously reported on SENCR, an lncRNA that seems to support the vascular smooth muscle cell (VSMC) contractile phenotype. However, information about VSMC-specific lncRNAs regulated by myocardin (MYOCD)/serum response factor, master switch for VSMC differentiation, is unknown.To define novel functions related we performed RNA sequencing in human coronary artery SMCs overexpress MYOCD. Several...
Down syndrome (DS), caused by trisomy of chromosome 21, occurs in 1 every 800 live births. Early defects cortical development likely account for the cognitive impairments DS, although underlying molecular mechanism remains elusive. Here, we performed histological assays and unbiased single-cell RNA-Seq (scRNA-Seq) analysis on cerebral organoids derived from 4 euploid cell lines induced pluripotent stem cells (iPSCs) 3 individuals with 21 to explore cell-type–specific abnormalities associated...
We are using induced pluripotent stem cell (iPSC) technology to study neuropsychiatric disorders associated with 22q11.2 microdeletions (del), the most common known schizophrenia (SZ)-associated genetic factor. Several genes in region have been implicated; a promising candidate is DGCR8, which codes for protein involved microRNA (miRNA) biogenesis. carried out miRNA expression profiling (miRNA-seq) on neurons generated from iPSCs derived controls and SZ patients del. Using thresholds of...
DNA methylation is a major epigenetic mechanism altering gene expression in development and disease. However, its role the regulation of during heart incompletely understood. The aim this study to reveal mouse embryonic hearts regulating development.We performed genome-wide profiling using methyl-sensitive, tiny fragment enrichment/massively parallel sequencing determine levels at ACGT sites. results showed that while global 1.64 million sites developing remains stable between day (E) 11.5...
The transition from meiotic spermatocytes to postmeiotic haploid germ cells constitutes an essential step in spermatogenesis. epigenomic regulatory mechanisms underlying this remain unclear. Here, we find a prominent transcriptomic switch the late early round spermatids during meiotic-to-postmeiotic transition, which is associated with robust histone acetylation changes across genome. Among deacetylases (HDACs) and acetyltransferases, that HDAC3 selectively expressed stages. Three...
Abstract Human stem cells and derivatives transplantation are widely used to treat nervous system diseases, while the fate determination of transplanted is not well elucidated. To explore cell changes human brain organoids before after transplantation, into prefrontal cortex (PFC) hippocampus (HIP), respectively. Single‐cell sequencing then performed. According time‐series sample comparison, mainly undergo neural development at 2 months post‐transplantation (MPT) glial 4MPT, A different...
Stochastic processes and imprinting, along with genetic factors, lead to monoallelic or allele-biased gene expression. expression fine-tunes information processing in immune cells the olfactory system, imprinting plays an important role development. Recent studies suggest that both stochastic events may be more widespread than previously considered. We are interested occurring brain because parent-of-origin effects suggestive of appear play a transmission schizophrenia (SZ) autism spectrum...
Thousands of pseudogenes exist in the human genome and many are transcribed, but their functional potential remains elusive understudied. To explore these issues systematically, we first developed a computational pipeline to identify transcribed from RNA-Seq data. Applying datasets 16 distinct normal tissues identified ∼3,000 that could produce non-coding RNAs manner low abundance high tissue specificity under physiological conditions. Cross-tissue comparison revealed transcriptional...
Transcription factors of the Sox protein family contain a DNA-binding HMG box and are key regulators progenitor cell fate. Here, we report that expression Sox30 is restricted to meiotic spermatocytes postmeiotic haploids. mutant males sterile due spermiogenic arrest at early round spermatid stage. Specifically, in absence Sox30, proacrosomic vesicles fail form single acrosomal organelle, spermatids step 2-3. Although most progress through meiosis, accumulation diplotene indicates delayed or...
Despite continuous expansion of the RNA binding protein (RBP) world, there is a lack systematic understanding RBPs in mammalian testis, which harbors one most complex tissue transcriptomes. We adapted interactome capture to mouse male germ cells, building an RBP atlas characterized by multiple layers dynamics along spermatogenesis. Trapping RNA-cross-linked peptides showed that glutamic acid-arginine (ER) patch, residue-coevolved polyampholytic element present coiled coils, enhances its host...
Schizophrenia (SZ) and autism spectrum disorders (ASD) are highly heritable neuropsychiatric disorders, although environmental factors, such as maternal immune activation (MIA), play a role well. Cytokines mediate the effects of MIA on neurogenesis behavior in animal models. However, stimulators can also induce febrile reaction, which could have independent through heat shock (HS)-regulated cellular stress pathways. this has not been well-studied. To help understand fever MIA, we used...
Recent studies have shown that the transcriptional functions of REST are much broader than repressing neuronal genes in non-neuronal systems. Whether occupies similar chromatin regions different cell types and how it interacts with other regulators to execute its a context-dependent manner has not been adequately investigated. We applied ChIP-seq analysis identify cistrome human CD4+ T cells compared published data from 15 types. found cistromes were distinct among types, binding several...
Induced pluripotent stem cell (iPSC) technology is providing an opportunity to study neuropsychiatric disorders through the capacity grow patient-specific neurons in vitro. Skin fibroblasts obtained by biopsy have been most reliable source of cells for reprogramming. However, using other somatic less invasive means would be ideal, especially children with autism spectrum (ASD) and neurodevelopmental conditions. In addition fibroblasts, iPSCs developed from cord blood, lymphocytes, hair...
ZNF804A (Zinc Finger Protein 804A) has been identified as a candidate gene for schizophrenia (SZ), autism spectrum disorders (ASD), and bipolar disorder (BD) in replicated genome wide association studies (GWAS) by copy number variation (CNV) analysis. Although its function not well-characterized, contains C2H2-type zinc-finger domain, suggesting that it DNA binding properties, consequently, role regulating expression. To further explore the of on expression downstream targets, we used...
Abstract Purpose: Uterine carcinosarcoma is a rare aggressive malignancy frequently presenting at advanced stage of disease with extrauterine metastases. Median survival less than 2 years due to high relapse rates after surgery and poor response chemotherapy or radiotherapy. The goal this study was identify novel therapeutic targets. Experimental Design: We applied RNA-seq analysis prospectively collected uterine tumor samples from patients undergoing primary surgical resection for...