A5034412953- Pelvic floor disorders treatments
- Urinary Bladder and Prostate Research
- Urinary Tract Infections Management
- Microfluidic and Capillary Electrophoresis Applications
- Advanced Proteomics Techniques and Applications
- Field-Flow Fractionation Techniques
- Microfluidic and Bio-sensing Technologies
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Mineral Processing and Grinding
- Electrochemical Analysis and Applications
- Regulation of Appetite and Obesity
- Bioinformatics and Genomic Networks
- thermodynamics and calorimetric analyses
- Nutrition, Genetics, and Disease
- Adipokines, Inflammation, and Metabolic Diseases
- Innovative Microfluidic and Catalytic Techniques Innovation
- Clinical Nutrition and Gastroenterology
- Electrohydrodynamics and Fluid Dynamics
- Genomics and Phylogenetic Studies
- Alzheimer's disease research and treatments
- Nanopore and Nanochannel Transport Studies
- Human Health and Disease
- Microbial Community Ecology and Physiology
- Electrowetting and Microfluidic Technologies
Arbor Research Collaborative for Health
2016-2025
Michigan United
2019-2022
Oklahoma State University
2021
Ann Arbor Center for Independent Living
2018-2019
NorthShore University HealthSystem
2018
Northwestern University
2018
University of Michigan
2018
Washington University in St. Louis
2018
St Petersburg University
2015
University of Miami
2008-2013
Quantitative proteomics analysis of cortical samples 10 Alzheimer's disease (AD) brains versus normally aged was performed by following the accurate mass and time tag (AMT) approach with high resolution LTQ Orbitrap spectrometer. More than 1400 proteins were identified quantitated. A conservative selecting only consensus results four normalization methods suggested used. total 197 shown to be significantly differentially abundant (p-values <0.05, corrected for multiplicity testing) in AD...
A new denoising and peak picking algorithm (MEND, matched filtration with experimental noise determination) for analysis of LC−MS data is described. The minimizes both random chemical in order to determine MS peaks corresponding sample components. Noise characteristics the set are experimentally determined used efficient denoising. MEND shown enable low-intensity be detected, thus providing additional useful information analysis. process denoising, performed chromatographic time domain, does...
Methanosarcina acetivorans produces acetate, formate, and methane when cultured with CO as the growth substrate [Rother M, Metcalf WW (2004) Proc Natl Acad Sci USA 101:], which suggests novel features of metabolism. Here we present a genome-wide proteomic approach to identify quantify proteins differentially abundant in response on versus methanol or acetate. The results indicate that oxidation CO2 supplies electrons for reduction methyl group by steps enzymes pathway determined other...
Major depressive disorder (MDD) is the most common psychiatric and second overall cause of disability. Even though a significant amount variance in MDD phenotype explained by inheritance, specific genetic variants conferring susceptibility to explain only minimal proportion causality. Moreover, genome-wide association studies have identified two small-sized effect loci that reach significance. In this study, group Mexican-American patients with controls recruited for pharmacogenetic study...
The phylum Myxococcota is a phylogenetically coherent bacterial lineage that exhibits unique social traits. Cultured are predominantly aerobic soil-dwelling microorganisms capable of predation and fruiting body formation.
We described and compared the frequency type of lower urinary tract symptoms reported by men women at time that they were recruited from urology urogynecology clinics into Symptoms Lower Urinary Tract Dysfunction Research Network multicenter, prospective, observational cohort study.
Abstract Aims The aims of this study were to assess the completeness voiding diaries in a research context and correlate diary data with patient‐reported questionnaires. Methods Men women enrolled Symptoms Lower Urinary Tract Dysfunction Research Network (LURN) given 3‐day fluid‐intake fill‐out. Diaries assessed for intake‐output imbalances. They assigned one four categories based on percentage missing fluid imbalance: no submitted, unusable (>40% void or intake volumes, unphysiological...
Our hypothesis is that changes in gene and protein expression are crucial to the development of late-onset Alzheimer’s disease. Previously we examined how DNA alleles control downstream RNA transcripts those relationships changed We have now proteins incorporated into networks two separate series evaluated our outputs different cell lines. pipeline included following steps: (i) predicting quantitative trait loci; (ii) determining differential expression; (iii) analysing transcript...
Due to the complexity of proteome samples, only a portion peptides and thus proteins can be identified in single LC-MS/MS analysis current shotgun proteomics methodologies. It has been shown that replicate runs used improve comprehensiveness analysis; however, high-intensity tend analyzed repeatedly different runs, reducing chance identifying low-intensity peptides. In contrast commonly online ESI-MS, offline MALDI decouples separation from MS acquisition, allowing in-depth selection for...
Leptin replacement rescues the phenotype of morbid obesity and hypogonadism in leptin-deficient adults. However, leptin's effects on insulin resistance are not well understood. Our objective was to evaluate leptin resistance. Three adults (male, 32 yr old, BMI 23.5 kg/m(2); female, 42 25.1 46 31.7 kg/m(2)) with a missense mutation gene were evaluated during treatment recombinant methionyl human (r-metHuLeptin). Insulin determined by euglycemic hyperinsulinemic clamps oral glucose tolerance...
Male urinary incontinence is thought to be infrequent. We sought describe the prevalence of in a male treatment seeking cohort enrolled LURN (Symptoms Lower Urinary Tract Dysfunction Research Network).
This paper presents application of sequential enhanced data processing procedures to high-resolution tandem mass spectra for identification peptides using the Mascot database search algorithm. A strategy (1) selection fragment ion peaks from MS/MS spectra, (2) utilization improved accuracy precursor ions, and (3) wavelet denoising prior have been developed. The number peptide identifications obtained was then compared with that software provided by instrument manufacturer. Approximately 9000...
A new algorithm is described for label-free quantitation of relative protein abundances across multiple complex proteomic samples. Q-MEND based on the denoising and peak picking algorithm, MEND, previously developed in our laboratory. takes advantage high resolution mass accuracy hybrid LTQ-FT MS spectrometer (or other high-resolution spectrometers, such as a Q-TOF MS). The strategy, termed "cross-assignment", introduced to increase substantially number quantitated proteins. In this...
Women with lower urinary tract symptoms are often diagnosed based on a predefined symptom complex or predominant symptom. There many limitations to this paradigm as patients present multiple which do not perfectly fit the preestablished diagnoses. We used cluster analysis identify novel, subtypes of women symptoms.We analyzed baseline questionnaire data obtained from 545 care seeking female participants enrolled in LURN (Symptoms Lower Urinary Tract Dysfunction Research Network)...
The impact of nonurological factors on male lower urinary tract symptoms (LUTS) remains unclear. We investigated cross-sectional and longitudinal associations among anxiety, depression, physical function, sleep quality symptom subdomains.Data from 518 men in the LURN (Symptoms Lower Urinary Tract Dysfunction Research Network) study were analyzed to identify between Patient-Reported Outcomes Measurement Information System® (PROMIS®) disturbance function measures LUTS subdomains, as derived...
Background and Aims: Detailed investigation of the biological pathways leading to hepatic fibrosis identification liver biomarkers may facilitate early interventions for pediatric cholestasis. Approach Results: A targeted enzyme‐linked immunosorbent assay–based panel nine (lysyl oxidase, tissue inhibitor matrix metalloproteinase (MMP) 1, connective growth factor [CTGF], IL‐8, endoglin, periostin, Mac‐2–binding protein, MMP‐3, MMP‐7) was examined in children with biliary atresia (BA; n =...
ABSTRACT Aims The goal of this study is to better understand the mechanisms and phenotypes urinary urgency through analysis voiding patterns clinical patients recorded in their bladder diaries. Methods A recently introduced, powerful dynamic approach was used analyze diaries a heterogeneous cohort 227 treated single practice. Individual were examined by performing intra‐subject correlation analyzes diary variables creating multivariable linear regression models. Individuals clustered based...
ABSTRACT Purpose To investigate sensory and motor function of the bladder urethra in women with without urgency urinary incontinence (UUI). Materials Methods Treatment‐seeking UUI healthy, asymptomatic, nontreatment seeking controls enrolled Symptoms Lower Urinary Tract Dysfunction Research Network Organ‐Based study (LURN‐Organ) underwent a single session physiological testing. Testing included rapid‐fill cystometry before after lidocaine administration, urethral pressure profiles, pelvic...
The goal of this study was the development N-terminal tags to improve peptide identification using high-throughput MALDI-TOF/TOF MS. Part 1 focused on influence derivatization intensities MALDI-TOF MS signals peptides. In part 2, various approaches for improvement fragmentation efficiency in are explored. We demonstrate that permanent cation tags, while significantly improving signal intensity mode, lead severe suppression MS/MS fragmentation, making these unsuitable analysis. present work,...
No AccessJournal of UrologyAdult Urology1 Jun 2019The Comprehensive Assessment Self-Reported Urinary Symptoms: A New Tool for Research on Subtypes Patients with Lower Tract Symptoms Kevin P. Weinfurt, James W. Griffith, Kathryn E. Flynn, David Cella, Tamara Bavendam, Jonathan B. Wiseman, Victor Andreev, H. Henry Lai, Alice Liu, Ziya Kirkali, Anne Cameron, and Catherine S. Bradley WeinfurtKevin Weinfurt *Correspondence: Department Population Health Sciences, Duke University School Medicine,...
Some patients continue to have bothersome lower urinary tract symptoms despite treatment. We examined characteristics associated with bother from in a prospective cohort.
The influence of the electroosmotic flow profile on efficiency and resolution capillary electrophoresis is studied. mathematical model formulated set equations solved numerically. results analysis are applicable to a wide range buffer concentrations diameters. temperature dependence electrophoretic mobility, viscosity thermal conductivity electrical ion concentration in double layer taken into account. It shown that there exists region diameters where much greater than mobility. especially...