A5101946411- Angiogenesis and VEGF in Cancer
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- MicroRNA in disease regulation
- Renal and related cancers
- Circular RNAs in diseases
- Pancreatic and Hepatic Oncology Research
- Cell death mechanisms and regulation
- Sirtuins and Resveratrol in Medicine
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Curcumin's Biomedical Applications
- Cancer, Lipids, and Metabolism
- RNA Research and Splicing
- Retinoids in leukemia and cellular processes
- PARP inhibition in cancer therapy
- Retinal Imaging and Analysis
- Synthesis of Tetrazole Derivatives
- Machine Learning in Healthcare
- Biological Stains and Phytochemicals
- Robotics and Automated Systems
- Electrolyte and hormonal disorders
- Cancer Genomics and Diagnostics
- Cell Adhesion Molecules Research
Hangzhou Medical College
2023-2025
Zhejiang Provincial People's Hospital
2023-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
China Medical University
2009-2022
Sheng Jing Hospital
2011-2012
Abstract Pancreatic cancer (PC) is a great health burden to patients owing its poor overall survival rate. Long noncoding RNAs (lncRNAs) interact with microRNAs (miRs) participate in tumorigenesis. Therefore, we aim uncover the role and related mechanism of LINC00473 PC through modulation miR‐195‐5p programmed death‐ligand 1 (PD‐L1). Increased PD‐L1 but declined were determined tissues cell lines, it was found that mainly situated cytoplasm. Also, verified bind both PD‐L1. Next, examine...
To investigate the pathobiological behaviors of gastric mixed-type (MT) carcinomas and carcinogenesis, clinicopathological characteristics MT were analyzed compared with intestinal-type (IT) diffuse-type (DT) carcinomas. The expression Ki-67, caspase-3, p53, fragile histine triad (FHIT), maspin, extracellular matrix metalloproteinase inducer (EMMPRIN), vascular growth factor (VEGF), MUC-2, 4, 5AC 6, CD44, E-cadherin, beta-catenin, phosphorylated glycogen synthase kinase 3beta-ser9...
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a member of the tumor factor family that selectively induces apoptosis in cancer cells. However, gastric cells are insensitive to TRAIL. In present study, we show oxaliplatin enhanced TRAIL-induced MGC803, BGC823, and SGC7901 Oxaliplatin promoted death receptor 4 (DR4) 5 (DR5) clustering into aggregated lipid rafts, while cholesterol-sequestering agent nystatin partially prevented raft aggregation, DR4 DR5 clustering, reduced...
We have investigated the synergistic effects of bFGF (basic fibroblast growth factor) and PDGF-BB (platelet-derived factor-BB) on proliferation, migration VEGF (vascular endothelial release EPCs (endothelial progenitor cells). The proliferation was assayed by MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium]. detected using Transwell system. Real-time PCR used to assess transcription PDGFRβ mRNA. PLC-γ (phospholipase C gamma) expression were...
Medulloblastoma is a primitive neuroectodermal tumor, which originates in the cerebellum, presumably due to alterations of some neurogenetic elements. Sirtuin 1 (SIRT1), class III histone deacetylase (HDAC), regulates differentiation neuronal stem cells but its status medulloblastomas remains largely unknown. The current study aimed address this issue by checking SIRT1 expression noncancerous cerebellar tissues, medulloblastoma tissues and established cell lines. roles proliferation survival...
The aim of this work is to evaluate the effects purified aromatic-turmerone(ar-turmerione, AR) on murine dendritic cells (DCs). These impacts AR DCs from bone marrow derived DCs(BMDCs) were assessed with use conventional scanning electron microscopy (SEM), fluorescence activated cell sorting (FACS), transmission (TEM), cytochemistry assay, FITC-dextran, bio-assay and enzyme linked immunosorbent assay (ELISA). We found that induced phenotypic maturation as evidenced by increased expression...
Previous reports have indicated a potential link between microRNA (miR)‑214 and hypoxia. In the present study, biological functions mechanisms of miR‑214 were determined, as well its correlation with HIF‑1α signaling in non‑small cell lung cancer (NSCLC) cells. Quantitative polymerase chain reaction revealed that expression was upregulated tissues compared adjacent normal tissues. mimics transfected into A549 cells, MTT, colony formation, invasion wound healing assays performed. It...
Wilms tumour is the most common of pediatric kidney. Elevation hypoxia-inducible factor 1α (HIF-1α) has been detected in 93% to 100% human specimens, suggesting a potential value HIF-1α as therapeutic target for tumour. In present study, stable HIF-1α-silenced cell strain was established by introducing short-hairpin RNA (shRNA) into SK-NEP-1 cells. Silencing significantly reduced single-cell growth capacity, suppressed proliferation and arrested cycle addition, reduction expression induced...
Abstract Background The associations between fasting blood glucose and staging overall survival of patients with pancreatic cancer are still controversial. This study aimed to investigate the association levels (OS) evaluate impact differentiation cancer. Methods was a retrospective pathologically confirmed admitted Shengjing Hospital China Medical University 01/2012 12/2016. outcome OS. factors associated OS were examined using univariable multivariable Cox logistic regression analyses....
Objective: To clarify the role and mechanism of GABPB1-AS1 in renal cell carcinoma.Methods: We collected 48 pairs tumor adjacent normal tissues from patients with clear carcinoma (ccRCC).Both 786-o caki-1 ccRCC lines were transfected GA-binding protein transcription factor subunit beta-1 antisense RNA 1 (GABPB1-AS1), miR-1246, or small interfering phosphoenolpyruvate carboxykinase (siPCK1) vectors.RNA expression was examined by quantitative reverse transcription-PCR Western blot.Cell...
SRY-box containing gene 17 (SOX17) was reported to be a candidate tumor suppressor in multiple tumors. Little is known about its role clear-cell renal cell carcinoma (ccRCC). This study aims identify the epigenetic regulation and tumor-suppressive function of SOX17 ccRCC.Fifty-five human ccRCC tissue samples, ten adjacent non-malignant kidney 20 paired paraffin section tissues seven RCC lines were obtained. Immunohistochemistry (IHC) real-time PCR used examine expression target genes at mRNA...
To identify the direct effects and functional mechanisms of SHP1, plus its relationship with STAT3 in pancreatic cancer.Immunohistochemistry Western blot assays were used to test SHP1 expression cancer. The functions cancer cells analyzed by cell viability, colony formation, flow cytometric analysis apoptosis, migration invasion assays. Co-immunoprecipitation, combined shotgun mass spectrometry, verified or indirect interactions JAK1 p-STAT3(Ser727). Non-labeling quantitative proteomics...
The development of intensive care medicine is inseparable from the diversified monitoring data. Intensive has been closely integrated with data since its birth. Critical research requires an integrative approach that embraces complexity critical illness and computational technology algorithms can make it possible. Considering need standardization application big in care, Care Medicine Branch China Health Information Big Data Society, Standard Committee convened expert group, secretary group...
The current study investigated the effects of granulocyte-macrophage colony-stimulating factor (GM-CSF) on rat endothelial progenitor cell (EPC) biology. EPCs were isolated from bone marrow and experiments carried out to determine dose- time-dependent GM-CSF angiogenesis, proliferation, viability, migration vasculogenesis. co- cultured with cardiac microvascular cells (CMECs) effect GM- CSF EPC angiogenesis in vitro. proliferation migration, vasculogenesis measured by respective application...
Elemene is a novel universal non-cytotoxic antitumor drug. Based on downregulation of VEGF expression, induction apoptosis vascular endothelial cells and suppression growth tumor cell, elemene becoming helpfully promising inhibitor angiogenesis, however, the detail mechanisms remains unclear needs to be further elucidated. Key words: Neoplasms; Neovascularization,pathologic; Suppression; Elemene
Background: Aberrant DNMT3a expression exists during the tumorigenesis of PDAC. However, it has not revealed accuracy role in prognosis PDAC patients due to limited number study population and lack basic experimental research. It is still great significance further investigate pathological characteristics function