A5003282336- Chronic Kidney Disease and Diabetes
- Renin-Angiotensin System Studies
- Inflammatory mediators and NSAID effects
- Eicosanoids and Hypertension Pharmacology
- Advanced Glycation End Products research
- Pancreatic function and diabetes
- Receptor Mechanisms and Signaling
- Hormonal Regulation and Hypertension
- Hepatocellular Carcinoma Treatment and Prognosis
- Ion Transport and Channel Regulation
- Estrogen and related hormone effects
- Nitric Oxide and Endothelin Effects
- Neurological Disease Mechanisms and Treatments
- Renal Diseases and Glomerulopathies
- Electrolyte and hormonal disorders
- Neuroendocrine regulation and behavior
- Diabetes and associated disorders
- Flavonoids in Medical Research
- Birth, Development, and Health
- Alcohol Consumption and Health Effects
- Apelin-related biomedical research
- Ginseng Biological Effects and Applications
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Calpain Protease Function and Regulation
- Drug Transport and Resistance Mechanisms
Gansu University of Traditional Chinese Medicine
2024
Eli Lilly (United States)
2008-2023
Nanjing University of Chinese Medicine
2020
Vanderbilt University
1999-2019
Shandong First Medical University
2016-2018
University of Jinan
2016-2018
Shandong Tumor Hospital
2016-2018
Shandong University
2016-2018
Dalian Medical University
2014-2015
First Affiliated Hospital of Dalian Medical University
2014-2015
Two nonradioactive methods for determining glomerular filtration rate (GFR) in conscious mice using FITC-labeled inulin (FITC-inulin) were evaluated. The first method measured GFR clearance kinetics of plasma FITC-inulin after a single bolus injection. Based on two-compartment model, estimated was 236.69 ± 16.55 and 140.20 22.27 μl/min male female C57BL/6J mice, respectively. Total or ⅚ nephrectomy reduced to 0 32.80 9.32 μl/min, Conversely, diabetes mellitus induced by streptozotocin...
Differential susceptibility to diabetic nephropathy has been observed in humans, but it not well defined inbred strains of mice. The present studies characterized the severity six mouse including C57BL/6J, DBA/2J, FVB/NJ, MRL/MpJ, A/J, and KK/HlJ Diabetes mellitus was induced using low-dose streptozotocin injection. Progression renal injury evaluated by serial measurements urinary albumin excretion, glomerular filtration rate (GFR), terminal assessment morphology over 25 weeks. Despite...
Therapeutic use of cyclooxygenase-inhibiting (COX-inhibiting) nonsteroidal antiinflammatory drugs (NSAIDs) is often complicated by renal side effects including hypertension and edema. The present studies were undertaken to elucidate the roles COX1 COX2 in regulating blood pressure function. inhibitors or gene knockout dramatically augment pressor effect angiotensin II (Ang II). Unexpectedly, after a brief increase, Ang was abolished deficiency (either inhibitor knockout). infusion also...
Therapeutic use of cyclooxygenase-inhibiting (COX-inhibiting) nonsteroidal antiinflammatory drugs (NSAIDs) is often complicated by renal side effects including hypertension and edema. The present studies were undertaken to elucidate the roles COX1 COX2 in regulating blood pressure function. inhibitors or gene knockout dramatically augment pressor effect angiotensin II (Ang II). Unexpectedly, after a brief increase, Ang was abolished deficiency (either inhibitor knockout). infusion also...
Superoxide excess plays a central role in tissue damage that results from diabetes, but the mechanisms of superoxide overproduction diabetic nephropathy (DN) are incompletely understood. In present study, we investigated enzyme dismutase (SOD), major defender against superoxide, kidneys during development murine DN. We assessed SOD activity and expression isoforms two mouse models (C57BL/6-Akita KK/Ta-Akita) exhibit comparable levels hyperglycemia different susceptibility to observed...
Clinical use of prostaglandin synthase-inhibiting NSAIDs is associated with the development hypertension; however, cardiovascular effects antagonists for individual receptors remain uncharacterized. The present studies were aimed at elucidating role E2 (PGE2) E-prostanoid receptor subtype 1 (EP1) in regulating blood pressure. Oral administration EP1 antagonist SC51322 reduced pressure spontaneously hypertensive rats. To define whether this antihypertensive effect was caused by inhibition, an...
Sodium caprate (C10) has been widely evaluated as an intestinal permeation enhancer for the oral delivery of macromolecules. However, effect C10 on absorption peptides with different physicochemical properties and its permeation-enhancing in vivo remains to be understood. Here, we effects rats a glucose-dependent insulinotropic polypeptide (GIP) glucagon-like peptide-1 (GIP-GLP1) dual agonist peptide (LY) semaglutide enzymatic stabilities self-association behaviors well exposure LY minipigs....
Macula densa (MD) cells express COX-2 and COX-2–derived PGs appear to signal the release of renin from renal juxtaglomerular apparatus, especially during volume depletion. However, synthetic machinery identity specific prostanoid released intact MD remains uncertain. In present studies, a novel biosensor tool was engineered directly determine whether PGE2 in response low luminal NaCl concentration ([NaCl]L). HEK293 were transfected with Ca2+-coupled E-prostanoid receptor EP1 (HEK/EP1) loaded...
Macula densa (MD) cells express COX-2 and COX-2-derived PGs appear to signal the release of renin from renal juxtaglomerular apparatus, especially during volume depletion.However, synthetic machinery identity specific prostanoid released intact MD remains uncertain.In present studies, a novel biosensor tool was engineered directly determine whether PGE 2 in response low luminal NaCl concentration ([NaCl] L ).HEK293 were transfected with Ca 2+ -coupled E-prostanoid receptor EP 1 (HEK/EP )...
Accumulating evidence indicates cyclooxygenase (COX) 1 and COX2 differentially regulate cardiovascular renal function. We have demonstrated previously in mice that inhibition enhances angiotensin II-induced hypertension, COX1 attenuates the pressor effect of II. To further elucidate mechanism underlying functional difference versus inhibition, present studies examined prostaglandin (PG) profiles derived COX1- or COX2-inhibited mouse kidney aorta using gas chromatographic/mass spectrometric...
The present studies examined the relationship between fasting blood glucose and Hb A(1c) in C57BL/6J, DBA/2J, KK/HlJ mice with without diabetes mellitus. Daily averaged levels based on continuous monitoring effects of 6-h vs. overnight were determined. highly correlated A(1c), as determined a hand-held automated device using an immunodetection method. R(2) values 0.90, 0.95, 0.99 KK/HIJ, respectively. Six-hour more closely level daily than did following fast. To validate...
Naringenin is a flavonoid polyphenolic compound, which facilitates the removal of free radicals, oxidative stress and inflammation. The present study aimed to obtain better understanding effects curcumin on regulation diabetes‑associated cognitive decline, its underlying mechanisms. An experimental diabetes mellitus (DM) rat model was induced by streptozoticin (50 mg/kg). Following treatment with naringin (100 200 mg/kg) for 16 weeks, body weight blood glucose levels DM rats were measured. A...
Paeoniflorin is one of the active ingredients commonly used herbal medicine derived from Paeonia, which exhibits anticancer properties. MicroRNA‑16 (miR‑16) upregulated in CD133- cells, but downregulated CD133+ cells glioma tissue. Matrix metalloproteinase‑9 (MMP‑9) expression tissue samples significantly higher than that healthy brain samples. Therefore, miR‑16 and MMP‑9 may be associated with pathogenesis. In present study, effects paeoniflorin on were analyzed. U87 treated at 0, 5, 10 20...
Abstract Aims/hypothesis The loss of pericytes surrounding the retinal vasculature in early diabetic retinopathy underlies changes to neurovascular unit that lead more destructive forms disease. However, it is unclear which pericytes. This study investigated hypothesis chronic increases one or inflammatory factors mitigate signalling pathways needed for pericyte survival. Methods Loss and levels markers at mRNA protein were two genetic models diabetes, Ins2 Akita/+ (a model type 1 diabetes)...
Astaxanthin (AST) is an oxygenated derivative of carotenoid, which possesses a strong antioxidant activity. AST can effectively remove active oxygen from the body, and thus considered to have important role in disease prevention treatment. The present study aimed determine effects on type 2 diabetic‑associated cognitive decline (DACD) rats. Rats were intraperitoneally injected with streptozotocin (STZ), order establish model diabetes mellitus (DM). A total 40 rats randomly divided into five...
Progress in research and developing therapeutics to prevent diabetic kidney disease (DKD) is limited by a lack of animal models exhibiting progressive disease. Chronic hypertension, driving factor progression human patients, lacking most available diabetes. We hypothesized that superimposition hypertension on mouse would accelerate DKD. To test this possibility, we induced persistent three type 1 diabetes two 2 adeno-associated virus delivery renin (ReninAAV). Compared with LacZAAV-treated...
Renal cortical cyclooxygenase-2 (COX-2) is restricted to the macula densa and adjacent thick ascending limbs (MD/cTALH). COX-2 increases in response diabetes renal ablation, both of which are characterized by hyperfiltration reduced NaCl delivery MD due increased proximal reabsorption. High-protein intake also induces decreases reabsorption proximally. We investigated whether high protein contributes protein-induced intrarenal renin biosynthesis. Cortical after loading but decreased...