A5073252783- Autophagy in Disease and Therapy
- Inflammasome and immune disorders
- Wound Healing and Treatments
- Spinal Cord Injury Research
- Toxoplasma gondii Research Studies
- Reconstructive Surgery and Microvascular Techniques
- Cell death mechanisms and regulation
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Heme Oxygenase-1 and Carbon Monoxide
- Sirtuins and Resveratrol in Medicine
- Vascular Procedures and Complications
- Bioactive Compounds in Plants
- Ubiquitin and proteasome pathways
- Gout, Hyperuricemia, Uric Acid
- Electrospun Nanofibers in Biomedical Applications
- Spine and Intervertebral Disc Pathology
- Bioactive Natural Diterpenoids Research
- Calcium signaling and nucleotide metabolism
- Ginger and Zingiberaceae research
- Periodontal Regeneration and Treatments
- Medicinal Plants and Bioactive Compounds
- Ion Channels and Receptors
- Advanced battery technologies research
- Family and Disability Support Research
First Affiliated Hospital Zhejiang University
2023-2025
Zhejiang University
2023-2025
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2020-2021
Wenzhou Medical University
2019-2021
Wenzhou City People's Hospital
2019-2021
Yangtze University
2019
Necrosis that appears at the ischemic distal end of random-pattern skin flaps increases pain and economic burden patients. Necroptosis is thought to contribute flap necrosis. Lysosomal membrane permeabilization (LMP) plays an indispensable role in regulation necroptosis. Nonetheless, mechanisms by which lysosomal membranes become leaky relationship between necroptosis lysosomes are still unclear flaps. Based on Western blotting, immunofluorescence, enzyme-linked immunosorbent assay, liquid...
Background: Caloric restriction mimetics (CRMs) mimic the favourable effects of caloric (CR) and have been shown to therapeutic in neuroinflammatory disease. However, whether CRMs improve functional recovery from spinal cord injury (SCI) underlying mechanism action remain unclear. In this study, we used 3,4-dimethoxychalcone (3,4-DC) evaluate value for SCI. Methods: HE, Masson Nissl staining; footprint analysis; Basso mouse scale were determine SCI after 3,4-DC treatment. RNA sequencing was...
Abstract Recent studies have shown that nucleophagy can mitigate DNA damage by selectively degrading nuclear components protruding from the nucleus. However, little is known about role of in neurons after spinal cord injury (SCI). Western blot analysis and immunofluorescence were performed to evaluate leakage SCI vivo NSC34 expression primary cultured with oxygen–glucose deprivation (OGD) vitro, as well interaction colocalization autophagy protein LC3 lamina Lamin B1. The effect UBC9, a...
Random-pattern skin flap is a vital technique frequently applied in reconstruction surgeries for its convenience and effectiveness solving defects. However, ischemic necrosis, especially the distal areas of flap, still needs extra attention after surgery. Earlier evidence has suggested that paeoniflorin (PF) could stimulate angiogenesis suppress cardiovascular disease. few studies have focused on role PF survival. In this study, we demonstrated human umbilical vein endothelial cells (HUVECs)...
Necrosis of random-pattern skin flaps limits their clinical application. Helix B surface peptide (HBSP) protects tissues from ischaemia-reperfusion injury but its short plasma half-life applications. Here, we have synthesized cyclic helix (CHBP) and investigated role in flap survival the underlying mechanisms.Flap viability was evaluated by area analysis, laser Doppler blood flow histological analysis. RNA sequencing used to identify mechanisms effects CHBP. Levels autophagy, oxidative...
Abstract A short peptide termed NEMO‐binding domain (NBD) has an inhibitory effect on nuclear factor kappa‐B (NF‐κB). Despite its efficacy in inhibiting inflammatory responses, the precise neuroprotective mechanisms of NBD spinal cord injury (SCI) remain unclear. This study aims to determine whether pyroptosis‐related aspects involved effects post‐SCI.Using RNA sequencing, molecular SCI are explored. The evaluation functional recovery is performed using Basso mouse scale, Nissl staining,...
After spinal cord injury, programmed cell death is common. In this context, autophagy plays a crucial role in clearing cellular debris, while necroptosis exacerbates neuroinflammation and further damages neural structures. The neuroprotective drug davunetide has shown substantial therapeutic effects on brain diseases, but its treating injury remains unclear. Therefore, the aim of study was to investigate after injury. To do this, we established mouse model contusion administered intranasally...
Osteoarthritis (OA) is a progressive illness that destroys cartilage. Oxidative stress major contributor of OA, while endoplasmic reticulum (ER) the key cellular damage under oxidative in chondrocytes. Echinacoside (ECH) main extract and active substance Cistanche, with potent antioxidative (OS) properties, currently clinical trials China. However, its function OA yet to be determined.We aimed explore specific role ECH occurrence development underlying mechanism vivo vitro.After mice were...
Spinal cord injury (SCI) refers to a major worldwide cause of accidental death and disability. However, the complexity pathophysiological mechanism can result in less‐effective clinical treatment. Growth differentiation factor 11 (GDF‐11), an antiageing factor, was reported affect development neurogenesis exert neuroprotective effect after cerebral ischaemic injury. The present work is aimed at investigating influence GDF‐11 on functional recovery following SCI, addition potential mechanisms...
Increasing evidence indicates that pyroptosis, a new type of programmed cell death, may participate in random flap necrosis and play an important role. ROS-induced lysosome malfunction is inducement pyroptosis. Transcription factor E3 (TFE3) exerts decisive effect oxidative metabolism lysosomal homeostasis. We explored the pyroptosis discussed TFE3 modulating Histological analysis via hematoxylin-eosin staining, immunohistochemistry, general evaluation flaps, tissue edema, laser Doppler...
Abstract Random‐pattern skin flaps are widely applied to rebuild and restore soft‐tissue damage in reconstructive surgery; however, ischemia subsequent ischemia‐reperfusion injury lead flap necrosis major complications. Exenatide, a glucagon‐like peptide‐1 analog, exerts therapeutic benefits for diabetic wounds, cardiac injury, nonalcoholic fatty liver disease. Furthermore, Exenatide is known activator of autophagy, which complex process subcellular degradation that may enhance the viability...
Random skin flaps are commonly used in reconstruction surgery. However, distal necrosis of the flap remains a difficult problem plastic Many studies have shown that activation autophagy is an important means maintaining cell homeostasis and can improve survival rate flaps. In current study, we investigated whether liraglutide promote random by stimulating autophagy. Our results show significantly viability, increase blood flow, reduce tissue oedema. addition, demonstrated stimulate...
Abstract Random skin flap grafting is the most common technique in reconstructive surgery. Despite progress techniques, incidence of distal necrosis still exceeds 3%, which limits its use clinical practice. Current methods for treating are lacking. Pinocembrin (Pino) can inhibit reactive oxygen species (ROS) and cell death a variety diseases, such as cardiovascular but role Pino random flaps has not been explored. Therefore, we explore how enhance survival specific upstream mechanisms via...
Abstract Spinal cord injury (SCI) is a severe to the central nervous system, and its treatment always major medical challenge. Proinflammatory cell death considered an important factor affecting neuroinflammation prognosis after injury. PANoptosis, newly discovered type of proinflammatory death, regulates activation executioner molecules apoptosis, pyroptosis necroptosis through PANoptosome, providing new target for therapeutic intervention SCI. However, role regulatory mechanism in SCI are...
Perforator flaps are commonly applied for a variety of skin defects. Many strategies (e.g., hyperbaric oxygen and preconditioning) have been investigated to improve flap survival, but postoperative 2.03 18.2 percent necrosis frequency remains major complication. The authors hypothesized that distal arterialized venous supercharged (DAVS) procedure might perfusion survival in an extended three-perforasome perforator rat model rescue ischemia intraoperatively.One hundred twenty male...
Abstract Magnetotactic bacteria are microaerobic microorganisms that take up iron from solution and crystallize magnetite nanoparticles called magnetosomes, enclosed by membrane intracellularly mainly formed the magnetosome membrane‐specific 6 ( Mms6 ) gene. M2 macrophages transfected magnetotactic bacteria‐derived gene to form magnetic bio‐nanoparticles. Under myelin debris‐rich hypoxic stress in spinal cord injury (SCI) microenvironment, ‐transfected can antagonize ferroptosis. Meanwhile,...
Background: Conjoined flap viability is associated with arterial blood supply and venous return. This study aimed to assess the effects of drainage position on return within conjoined flap. Methods: Fifty-four rats were divided randomly into three groups ( n = 18 per group). In experimental group 2, only right intercostal posterior artery left iliolumbar vein maintained; meanwhile, preserved in 1. The control had preserved. distances between angiosomes measured. At 7 days after surgery,...
Abstract Circular RNAs (circRNAs) play a role in sepsis-related autophagy. However, the of circRNAs autophagy after sepsis-induced cardiomyopathy (SICM) is unknown, so we explored circRNA expression profiles associated with an acute sepsis mouse model. At dose 10 mg/kg, mice were intraperitoneally administered lipopolysaccharides. The myocardial tissue was harvested 6 h for microarray analysis, qRT-PCR, and western blotting. Gene Ontology, Kyoto Encyclopedia Genes Genomes Set Enrichment...
Random-pattern skin flap replantation is generally used in the reconstruction of surgical tissues and covering a series defects. However, ischemia often occurs at distal parts, which lead to necrosis. Previous studies have shown that andrographolide (Andro) protects against ischemic cardiovascular diseases, but little known about effect Andro on viability. Thus, our study aimed building model random-pattern understand mechanism Andro-induced effects survival. In this study, fifty-four mice...
Objectives There is a high risk of nerve root injury during endoscopic‐assisted transforaminal lumbar interbody fusion (Endo‐TLIF). This study used computed tomography (CT) imaging to assess the relationship between exiting and its surroundings, corresponding intervertebral disc. We also measured approximate position angle for placement working cannula reduce Endo‐TLIF procedures in Chinese population. Methods retrospective was conducted at our institution December 2021 2022. A total 115...