A5100678634- RNA modifications and cancer
- HIV Research and Treatment
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- HIV/AIDS drug development and treatment
- Advanced biosensing and bioanalysis techniques
- Cytomegalovirus and herpesvirus research
- Bioinformatics and Genomic Networks
- Ferroptosis and cancer prognosis
- Cancer, Hypoxia, and Metabolism
- Cancer, Lipids, and Metabolism
- Single-cell and spatial transcriptomics
- Genomics and Chromatin Dynamics
- Biomarkers in Disease Mechanisms
- Escherichia coli research studies
- Epigenetics and DNA Methylation
- Bacteriophages and microbial interactions
- CAR-T cell therapy research
- Genetics, Bioinformatics, and Biomedical Research
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Machine Learning in Bioinformatics
- Pluripotent Stem Cells Research
- Fibroblast Growth Factor Research
- Student Assessment and Feedback
Zhengzhou People's Hospital
2023-2025
Sun Yat-sen University
2020-2024
Sun Yat-sen University Cancer Center
2020-2024
Nanjing Medical University
2023-2024
The First Affiliated Hospital, Sun Yat-sen University
2020-2022
Kunming Institute of Zoology
2022
Chinese Academy of Sciences
2022
University of Chinese Academy of Sciences
2022
Beijing Foreign Studies University
2022
Guangdong Pharmaceutical University
2022
Abstract Distinguishing the few disease-related variants from a massive number of passenger is major challenge. Variants affecting RNA modifications that play critical roles in many aspects metabolism have recently been linked to human diseases, such as cancers. Evaluating effect genetic on will provide new perspective for understanding pathogenic mechanism diseases. Previously, we developed database called ‘m6AVar’ host associated with m6A, one most prevalent eukaryotes. To all modification...
Abstract The visualization of biological sequences with various functional elements is fundamental for the publication scientific achievements in field molecular and cellular biology. However, due to limitations currently used applications, there are still considerable challenges preparation schematic diagrams. Here, we present a professional tool called IBS 2.0 illustrating organization both protein nucleotide sequences. With abundant graphical provided 2.0, can be easily represented...
High-quality schematic illustrations are fundamental to the publication of scientific achievements in biomedical research, which crucial for effectively conveying complex concepts. However, creating such remains challenging many researchers due need devote a significant amount time and effort accomplish it. To address this need, we present Generic Diagramming Platform (GDP, https://BioGDP.com), comprehensive database professionally crafted graphics (bio-graphics). Currently, GDP houses 7 562...
Immunotherapy is a promising cancer treatment method; however, only few patients benefit from it. The development of new immunotherapy strategies and effective biomarkers response resistance urgently needed. Recently, high-throughput bulk single-cell gene expression profiling technologies have generated valuable resources. However, these resources are not well organized systematic analysis difficult. Here, we present TIGER, tumor resource, which contains transcriptome data 1508 samples with...
RNA modification is a dynamic and reversible process regulated by series of writers, erasers readers (WERs). Abnormal changes WERs will disrupt the homeostasis their target genes, leading to dysregulation metabolisms such as stability translation, consequently diseases cancer. A public repository hosting regulatory relationships between genes help in understanding roles modifications various physiological pathological conditions. Previously, we developed database named 'm6A2Target' host...
As an increasing number of noncoding RNAs (ncRNAs) have been suggested to encode short bioactive peptides in cancer, the exploration ncRNA-encoded small (ncPEPs) is emerging as a fascinating field cancer research. To assist studies on regulatory mechanisms ncPEPs, we describe here database called SPENCER (http://spencer.renlab.org). Currently, has collected total 2806 mass spectrometry (MS) data points from 55 studies, covering 1007 tumor samples and 719 normal samples. Using MS-based...
A general strategy to improve the transfection efficiency as well lower cytotoxicity for polycationic vectors has been developed. Through polycondensation addition of N,N′-methylene bisacrylamide and 1-(2-aminoethyl)piperazine in a water/N,N-dimethylformamide cosolvent, series cationic poly(amido amine)s with same repeating units but different branched architecture have prepared. With increase architecture, polymers become more compact, accompanied by enhancement primary tertiary amino...
Abstract TIMP-1 (Tissue Inhibitor of Metalloproteinases-1) is a protein involved in regulating extracellular matrix (ECM) degradation. It recognized as significant biomarker for cancer diagnosis. This study aimed to develop and characterize single-stranded DNA (ssDNA) aptamer targeting human with high affinity specificity. A magnetic beads-based SELEX process combined qPCR was used select aptamers over seven rounds. The enriched ssDNA library analyzed using high-throughput sequencing...
Abstract Liquid–liquid phase separation (LLPS) is critical for assembling membraneless organelles (MLOs) such as nucleoli, P-bodies, and stress granules, which are involved in various physiological processes pathological conditions. While the role of RNA formation maintenance MLOs increasingly appreciated, there still a lack specific resources LLPS-related RNAs. Here, we presented RPS (http://rps.renlab.org), comprehensive database RNAs 20 distinct biomolecular condensates from eukaryotes...
The caudal-related homeobox geneCdx-2/3 is a critical "master" control gene in embryogenesis. Mice heterozygous for null mutation inCdx-2/3 exhibit multiple malfunctions including tail abnormalities, stunted growth, homeotic shift vertebrae, and the development of intestinal adenomatous polyps, indicating thatCdx-2/3 haplo-insufficient. In vitro studies have identified more than half-dozen downstream target genes expressed pancreatic cells this transcription factor. We examined...
Magnetic nonviral gene vectors were in situ prepared the presence of ferrous salts and hyperbranched poly(ethylenimine)s (HPEI) with different molecular weights. HPEI, one most promising vectors, was not only utilized as nanoreactor stabilizer to prepare magnetic nanoparticles, but also skillfully used a base supplier avoid introducing alkali hydroxide or ammonia. various magnetite contents saturation magnetizations obtained by changing weight ratio HPEI FeSO4·7H2O HPEI. MTT assays suggested...
2'-O-methylations (2'-O-Me or Nm) are one of the most important layers regulatory control over gene expression. With increasing attentions focused on characteristics, mechanisms and influences 2'-O-Me, a revolutionary technique termed Nm-seq were established, allowing identification precise 2'-O-Me sites in RNA sequences with high sensitivity. However, as costs complexities involved this new method, large-scale detection in-depth study is still largely limited. Therefore, development novel...
Systemic infections in avian species caused by pathogenic Escherichia coli (APEC) are economically devastating to poultry industries worldwide. To unravel factors possibly involved APEC pathogenicity, suppression subtractive hybridization was applied, leading the identification of a putative autotransporter adhesin gene aatA our previous study. In this study, mechanism AatA further determined. A deletion mutant constructed DE205B, which results reduced capacity adhere DF-1 cells, defective...
Sanhua decoction (SHD) is a famous classic Chinese herbal prescription for ischemic stroke, and aquaporin 4 (AQP4) reported to play key role in brain edema. This study aimed investigate neuroprotection of SHD against focal cerebral ischemia/reperfusion (I/R) injury rats explore the hypothesis that AQP4 probably target I/R rats. Lentiviral-mediated AQP4-siRNA was inducted into adult male Sprague-Dawley via intracerebroventricular injection. The model established by occluding middle artery....
The CONSTANS (CO)-like gene family has been well studied for its role in the regulation of plant flowering time. However, their remains poorly understood cotton. To better understand possible roles CO-like cotton, we performed a comprehensive genome-wide analysis genes Phylogenetic tree showed that naturally clustered into three groups. Segmental duplication and whole genome (WGD), which occurred before polyploidy, were important contributors to expansion within At ("t" indicates tetraploid)...
The dual phosphatase CDC25 has recently been identified as a target for diverse triple-negative breast cancers including RB1/PTEN/P53-deficient tumors. Moreover, inhibitors effectively synergize with PI3K to suppress tumor growth. We discuss these findings and the challenges that lie ahead in bringing clinic.
Abstract Immunotherapy is a promising cancer treatment method, but only few patients benefit from it. Developing new immunotherapy strategies and effective biomarkers of response resistance urgent. Recent high-throughput bulk single-cell gene expression profiling technologies have generated valuable resources in these regards. However, are not well organized systematic analysis difficult. Here, we present TIGER, tumor resource, which contains transcriptome data 1508 samples with clinical...
Abstract Cis-regulatory elements (CREs) and super cis-regulatory (SCREs) are non-coding DNA regions which influence the transcription of nearby genes play critical roles in development. Dysregulated CRE SCRE activities have been reported to alter expression oncogenes tumor suppressors, thereby regulating cancer hallmarks. To address strong need for a comprehensive catalogue dysregulated CREs SCREs human cancers, we present TSCRE (http://tscre.zsqylab.com/), an open resource providing...
Evaluating the impact of genetic variants on RNA modifications (RMs) is crucial for identifying disease-associated and understanding pathogenic mechanisms underlying human diseases. Previously, we developed a database called RMVar to catalog linked in humans mice. Here, present an updated version 2.0 (http://rmvar.renlab.cn). In this version, applied enhanced analytical pipeline latest modification datasets variant information identify RM-associated variants. A notable advancement our...