Our pilot study using miRNA PCR array found that miRNA-29b (miR-29b) is differentially expressed in primary cultured CD133-positive A549 cells compared with CD133-negative cells. Ten human non-small cell lung cancer (NSCLC) lines and samples from thirty patients NSCLC were analyzed for the expression of miR-29b by quantitative RT-PCR. Bioinformatics analysis combined tumor metastasis showed potential target genes miR-29b. lentivirus inhibitors transfected into to investigate its role on...

In this study, Ganoderma lucidum crude polysaccharide (GLP) was found to have protective effect on liver damage in mice caused by restraint stress through improving oxidative status. Two polysaccharides, including a neutral β-glucan (GLPB2) and an acidic (GLPC2) were purified from GLP anion-exchange chromatography (AEC) combined with gel permeation. GLPC2, average molecular weight of 20.56 kDa, exhibited stronger hepatoprotective against H2O2-induced injury HepG2 cells compared GLPB2....

Hydrogen sulfide releasing agents (or H2S donors) have been recognized gasotransmitters with potent cytoprotective and anticancer properties. However, the clinical application of donors has hampered by their fast H2S-release, instability, lack tumor targeting, despite unclear molecular mechanism action. Here we rationally designed an amphiphilic pentapeptide (RGDFF) to coassemble de novo thiol-activated (CL2/3) into nanocarriers for targeted therapy non-small-cell lung cancer, which proved...

Nonsmall cell lung cancer (NSCLC), which accounts for the majority of cancer-related deaths globally, remains refractory to available therapies despite advancements in treatment modalities. Hence, there is a pressing need development safer and more efficacious combination therapeutic strategies. Barbiturate derivatives have emerged as promising candidates chemotherapy. In this study, we introduced JL1-MOF/PNIPAM (JMP), nanomedicine platform that encapsulates JL1, barbituric acid derivative,...

Programmed cell death-1 (PD-1) inhibitors and anti-angiogenic drugs have become a hotspot in research of anti-tumor programs; however, they can also cause some rare drug-related adverse reactions. Immune checkpoint (ICIs) reactions the body, collectively known as immune-related events (irAEs). Ocular side effects occur both targeted immunotherapy patients, including dry eye, blurred vision, uveitis, conjunctivitis, retinopathy, or thyroid eye disease. To our knowledge, this is first case...

Epstein–Barr virus-encoded latent membrane protein-1 (LMP1) plays a fundamental role in the malignant transformation of nasopharyngeal carcinoma (NPC), although mechanism is not well understood. Here, we showed that Limb-bud and Heart (LBH) considerably downregulated patient NPC tissues. The expression LBH biopsies 40 consecutive patients devoid initial distant metastasis treated according to consistent guidelines was also analyzed found level correlated with some clinicopathological...

Annexin A1 (ANXA1) is a member of the annexin superfamily. Previous studies have reported that ANXA1 highly expressed in various types malignant tumor; however, its role progression non‑small cell lung cancer (NSCLC) remains to be fully clarified. The present study aimed investigate oncogenic NSCLC cells vitro. RNA interference was used downregulate expression A549 and H1299 using small interfering lentiviral vector. Subsequently, proliferation migration were detected Cell Counting kit‑8,...

Epithelial-to mesenchymal transition (EMT) involves in metastasis, causing loss of epithelial polarity. Metastasis is the major cause carcinoma-induced death, but mechanisms are poorly understood. Here we identify differentially expressed adenocarcinoma lung-1 (DAL-1), a protein belongs to membrane-associated cytoskeleton 4.1 family, as an efficient suppressor EMT lung cancer.The relationship between DAL-1 and markers were analyzed by using immunohistochemistry clinical cancer tissues....

Immunization with synthetic, preaggregated β-amyloid (Aβ) was the first treatment approach able to dramatically reduce brain Aβ pathology in Alzheimer's disease (AD) animal models. For development of a safe vaccine, we investigated whether 4Aβ1-15 (four tandem repeats GPGPG-linked Aβ1-15 sequences) had therapeutic effects APP/PS1 transgenic mice model AD. We described production anti-Aβ antibodies immunized mixed MF59 adjuvant. The antibody concentrations were increased which bound AD...

Cleavage polyadenylation specificity factor (CPSF) is the core component of 3'-end processing complex, which determines site cleavage interactions specific sequence elements within pre-mRNAs. The present study revealed that all members CPSF complex were overexpressed in lung cancer tissue from Cancer Genome Atlas (TCGA) Lung Cohort compared with normal tissue. Analysis overall survival and recurrence-free verified only CPSF3 was associated prognosis recurrence adenocarcinoma (LUAD), thus...

Abstract Background Age-related macular degeneration (AMD) is a leading cause of vision loss in elderly people, and dry AMD the most common type AMD. Oxidative stress alternative complement pathway activation may play essential roles pathogenesis There are no available drugs for Qihuang Granule (QHG) an herbal formula treatment AMD, it achieves good clinical effect our hospital. However, its potential mechanism unclear. Our study investigated effects QHG on oxidative stress-associated...

As important messengers of intercellular communication, exosomes can regulate local and distant cellular communication by transporting specific exosomal contents also promote or suppress the development progression gastric cancer (GC) regulating growth proliferation tumor cells, tumor-related immune response angiogenesis. Exosomes transport bioactive molecules including DNA, proteins, RNA (coding noncoding) from donor cells to recipient causing reprogramming target cells. In this review, we...

Sepsis-induced acute kidney injury (AKI) is considered as a life-threatening complication of sepsis. The purpose this study to clarify the involvement annexin A3 (ANXA3) in sepsis-related AKI. Lipopolysaccharide (LPS) was used establish cell model based on HK2 cells. ANXA3 expression quantified through quantitative real-time polymerase chain reaction. Cell proliferative capacities were assessed 5-ethynyl-2'-deoxyuridine proliferation, counting kit-8, and colony formation experiments. Flow...

To investigate the molecular mechanisms of gastric carcinogenesis.We used label-free quantification technology integrated with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis to identify differentially expressed proteins in 160 specimens normal mucosa, mucosa mild dysplasia, moderate severe and early mucosal cancer (GC) collected at Second Hospital Lanzhou University from 2010 2015. Immunohistochemistry was verify detected by LC-MS/MS.With a threshold 1.2-fold change...

Background Annexin 1 (ANXA1) expression is associated with the malignant tumor phenotype, making it an attractive therapeutic target. However, little known about regulation of ANXA1 in non‐small cell lung cancer (NSCLC). Methods We investigated biological roles growth, migration, and invasion, explored possibility as a potential target for treatment NSCLC. Results Our findings revealed that enhanced nuclear factor (NF)‐κB activation NSCLC cells by interaction inhibitor NF‐κB kinase complex...

Induction of an immune response to amyloid-β (Aβ) protein is effective in treating animal models Alzheimer’s disease. The Aβ1–15 sequence contains the antibody epitope(s), but lacks T-cell reactive sites full-length Aβ1–42. We tested two alternative peptide immunogens encompassing either a tandem repeat GPGPG-linked sequences (2Aβ15-linker) or without spacer (2Aβ15). Titers immunized sera were measured by indirect ELISA. analyzed production interferon-γ and interleukin-4 cytokine lymphocytes...

Chronic myelogenous leukemia (CML) is a malignant tumor formed by the clonal proliferation of bone marrow hematopoietic stem cells. CML relatively rare disease, mainly affecting elderly patients, but prevalence expected to increase dramatically. The tyrosine kinase inhibitors (TKIs) have changed patients' treatment patterns and improved its effect, drug resistance still remains significant problem be solved. Therefore, identification biomarkers involved therein essential for prognosis...

A mycochemical investigation on the medicinal mushroom Amauroderma rugosum led to isolation of 30 compounds, including 14 sterols, 6 phenolic constituents, 5 unsaturated fatty acids, and other compounds. The structures these compounds were elucidated by comparison their nuclear magnetic resonance spectroscopic mass spectrometry data with literature data. Among them, compound 27 was obtained as a new natural compound, 2-4, 7-13, 15-30 isolated from genus for first time. Sterols acids showed...

We aimed to create transgenic (Tg) mice engineered for magnetic resonance imaging (MRI). To ascertain if MagA expression contributes oxidative stress and iron metabolism, we report the generation of Tg in which ubiquitous can be detected by MRI vivo. Expression diverse tissues was assessed, accumulation deposition nanoparticles were analyzed. Levels antioxidant enzymes, lipid peroxidation cytokine production determined, metabolism-related proteins also detected. showed no apparent pathologic...

The binding mechanism of 7-ethyl-10-hydroxycamptothecin (SN38) with β-lactoglobulin (β-lg) was studied by molecular docking and dynamics simulation. results showed that van der Waals forces hydrogen bonds played an important role in maintaining the stability β-lg/SN38 complex. simulation RMSD (root-mean-square deviation) value complex system reached equilibrium after 38000 ps Moreover, calculation also SN38 more likely to bind hydrophobic cavity than other sites β-lg. provided a new clue for...