A5060290732- Mass Spectrometry Techniques and Applications
- Immune Cell Function and Interaction
- Protein Interaction Studies and Fluorescence Analysis
- Molecular Sensors and Ion Detection
- Adipose Tissue and Metabolism
- Integrated Energy Systems Optimization
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Hybrid Renewable Energy Systems
- Diabetes and associated disorders
- HIV Research and Treatment
- Analytical Chemistry and Chromatography
- Microfluidic and Capillary Electrophoresis Applications
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Biochemical and Structural Characterization
- Immune Response and Inflammation
- Mitochondrial Function and Pathology
- Reproductive System and Pregnancy
- Lipid metabolism and biosynthesis
- Microfluidic and Bio-sensing Technologies
- Antimicrobial Peptides and Activities
- Geothermal Energy Systems and Applications
- Radical Photochemical Reactions
- Renewable energy and sustainable power systems
Southwest Jiaotong University
2025
Jackson Laboratory
2025
Ascension Providence Hospital
2024
Bengbu Medical College
2023
Second Xiangya Hospital of Central South University
2015-2023
Central South University
2015-2023
Columbia University Irving Medical Center
2023
Center for Excellence in Molecular Cell Science
2023
University of Chinese Academy of Sciences
2023
Emory University
2019-2020
Significance Antiinfectives, drugs that inhibit virulence strategies of microbial pathogens without affecting bacterial growth, may prevent hospital-acquired infections caused by antibiotic-resistant Staphylococcus aureus . We used virtual screening and synthetic optimization to identify 3,6-disubstituted triazolothiadiazole compounds as inhibitors sortase, an enzyme incorporates surface proteins into the staphylococcal envelope. Other Gram-positive bacteria also use sortase for protein...
Purpose: To determine plasma metabolite and metabolic pathway differences between patients with type 2 diabetes diabetic retinopathy (DR) without (diabetic controls), proliferative DR (PDR) nonproliferative (NPDR). Methods: Using high-resolution mass spectrometry liquid chromatography, untargeted metabolomics was performed on samples from 83 90 controls. Discriminatory features were identified through partial least squares discriminant analysis, linear regression used to adjust for age, sex,...
Ginsenoside metabolite compound K (CK) is an active produced by ginsenosides in vivo that has anti-arthritic effect related to the glucocorticoid receptor (GR). However, potential mechanisms of CK remain unclear.This study explores role and vitro.Adjuvant arthritis (AA) model was induced Sprague-Dawley (SD) rats; rats were randomly divided into four groups (n = 10): normal, AA, (80 mg/kg), dexamethasone (Dex) group (1 mg/kg). From day 15, treated with (once a day, i.g.) Dex every 3 days,...
The immune response to live-attenuated Francisella tularensis vaccine and its host evasion mechanisms are incompletely understood. Using RNA-Seq LC–MS on samples collected pre-vaccination at days 1, 2, 7, 14 post-vaccination, we identified differentially expressed genes in PBMCs, metabolites serum, enriched pathways, that correlated with T cell B responses, or gene expression modules. While an early activation of interferon α/β signaling was observed, several innate pathways including TLR,...
During the lytic phase of Epstein-Barr virus (EBV), binding transactivator Zta to origin replication (oriLyt) and BHLF1 transcript, forming a stable RNA-DNA hybrid, is required initiate viral DNA replication. EBV-encoded proteins form complexes amplify DNA. BMRF1, polymerase accessory factor, essential for also known as transcriptional regulator expression BALF2 (single-stranded [ssDNA]-binding protein). In order determine systematically how BMRF1 regulates transcription, knockout bacmid was...
A novel electrochemical assay for DNA ligase activity is described. The exploits the properties of hairpins tethered at one terminus to a gold electrode and labelled other with ferrocene group rapid characterisation status by cyclic voltammetry. Successful ligation 'nicked' indicated retention couple when exposure followed conditions that denature hairpin. results demonstrate simplicity integrating detection hairpin based biosensors illustrate new approach ligases, which NAD+-dependent...
Peptide radical cations A(n)Y(•+) (where n = 3, 4, or 5) and A5W(•+) have been generated by collision-induced dissociation (CID) of [Cu(II)(tpy)(peptide)](•2+) complexes. Apart from the charge-driven fragmentation at N-Cα bond hetero residue producing either [c + 2H](+) [z - H](•+) ions radical-driven Cα-C to give a(+) ions, unusual product [x are abundant in CID spectra peptides with second third position chain. The formation these requires that both charge be located on peptide backbone....
Objective: To explore correlations between mRNA (hA3G, hA3F, and hA3B) levels CD4+ T-cell counts HIV-1 viral loads to evaluate their respective roles in disease progression. Methods: Real-time polymerase chain reaction was used quantify the of hA3G, hA3B, hA3F peripheral blood mononuclear cells from slow progress patients (SP), asymptomatic HIV-infected (AS), AIDS patients, HIV-negative controls. Results: The hA3G hA3B correlated positively with (r = 0.436, P 0.002, r 0.334, 0.025),...
Abstract Electrophoretic field gradient focusing has been used to separate the two oxidation states of myoglobin (Mb), and Mb from bromophenol blue (BPB). Polyacrylamide Sephadex were shown be suitable packing materials whilst silica led band broadening with Mb. BPB could simultaneously focused apart using either a fixed 21‐electrode setup or dynamic 6‐electrode setup. Using three‐electrode analyte but not both simultaneously. It was that higher ionic strength buffer in separation channel...
The collision-induced dissociation (CID) of [b5 - H]˙(+) ions containing four alanine residues and one tryptophan give identical spectra regardless the initial location indicating that, as proposed for b5(+) ions, sequence scrambling occurs prior to dissociation. Cleavage predominantly at peptide bonds N-Cα bond residue that is attached N-terminus residue. product latter pathway, an ion m/z 240, base peak in all mass spectra. With exception minor channel giving a b3(+) ion, retain both...