A5075239306- Glioma Diagnosis and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Chemokine receptors and signaling
- Peptidase Inhibition and Analysis
- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Neurogenesis and neuroplasticity mechanisms
- CAR-T cell therapy research
- Cell Adhesion Molecules Research
- MicroRNA in disease regulation
- Neuroscience and Neuropharmacology Research
- Olfactory and Sensory Function Studies
- RNA modifications and cancer
- Extracellular vesicles in disease
- Axon Guidance and Neuronal Signaling
- Biochemical Analysis and Sensing Techniques
- Neuroscience and Neural Engineering
- Epigenetics and DNA Methylation
- Ferroptosis and cancer prognosis
- Receptor Mechanisms and Signaling
- Constructed Wetlands for Wastewater Treatment
- Zebrafish Biomedical Research Applications
Baylor College of Medicine
2017-2025
Kennedy Space Center
2024
Baylor Genetics
2020-2023
Salisbury University
2016
Neuronal activity drives alterations in gene expression within neurons, yet how it directs transcriptional and epigenomic changes neighboring astrocytes functioning circuits is unknown. We found that neuronal induces widespread up-regulation down-regulation astrocytes, highlighted by the identification of
Astrocytes are the most abundant type of glial cell in central nervous system and perform a myriad vital functions, however, nature their diversity remains longstanding question neuroscience. Using transcription factor motif discovery analysis on region-specific gene signatures from astrocytes we uncovered universal expression profiles. This revealed that motifs for Nuclear Factor-I (NFI) present genes enriched all regions, with NFIB NFIX exhibiting pan-astrocyte olfactory bulb, hippocampus,...
Abstract Background Glioblastoma is the most common and aggressive type of primary brain tumor, as patients succumb to disease less than two years after diagnosis. Critically, studies demonstrate that glioma recruits surrounding blood vessels, while some work suggests tumor stem cells themselves directly differentiate into endothelial cells, yet molecular cellular dynamics endothelium in are poorly characterized. The goal this study was establish morphological benchmarks for associated...
Abstract The role of transcription factors during astrocyte development and their subsequent effects on neuronal has been well studied. Less is known about astrocytes contributions towards circuits behavior in the adult brain. Astrocytes play important roles synaptic modulation, however sensory function maintenance circuit architecture remain unclear. Here, we show that loss factor Sox9 results both anatomical functional changes mouse olfactory bulb (OB) astrocytes, affecting processing....
Non-canonical roles for growth factors in the nucleus have been previously described, but their mechanism of action and biological remain enigmatic. Platelet-derived factor B (PDGFB) can drive formation low-grade glioma here we show that it localizes to human cells where binds chromatin preserve genome stability cell lineage. Failure PDGFB localize leads chromosomal abnormalities, aberrant heterochromatin architecture accelerated tumorigenesis. Furthermore, nuclear localization is reliant...
Glioma is a rare brain tumor with poor prognosis. Familial glioma subset of strong genetic predisposition that accounts for approximately 5% cases. We performed whole-genome sequencing on an exploratory cohort 203 individuals from 189 families history familial and additional validation 122 115 families. found significant enrichment deleterious variants seven genes in both cohorts, the most significantly enriched gene was
Epigenetic dysregulation is a universal feature of cancer that results in altered patterns gene expression drive malignancy. Brain tumors exhibit subtype-specific epigenetic alterations; however, the molecular mechanisms responsible for these diverse states remain unclear. Here, we show developmental transcription factor Sox9 differentially regulates epigenomic high-grade glioma (HGG) and ependymoma (EPN). Using our autochthonous mouse models, found suppresses HGG growth expands associated...
Abstract Prior studies have described the complex interplay that exists between glioma cells and neurons, however, electrophysiological properties endogenous to tumor remain obscure. To address this, we employed Patch-sequencing on human specimens found one third of patched in IDH mutant (IDH mut ) tumors demonstrate both neurons glia by firing single, short action potentials. define these hybrid (HCs) discern if they are origin, developed a computational tool, Single Cell Rule Association...
Neural tube closure is a critical early step in central nervous system development that requires precise control of metabolism to ensure proper cellular proliferation and differentiation. Dysregulation glucose during pregnancy has been associated with neural defects (NTDs) humans suggesting the developing neuroepithelium particularly sensitive metabolic changes. However, it remains unclear how pathways are regulated neurulation. Here, we used single-cell mRNA-sequencing analyze expression...
Astrocytes play central roles in normal brain function and are critical components of synaptic networks that oversee behavioral outputs. Despite their close affiliation with neurons, how neuronal-derived signals influence astrocyte at the gene expression level remains poorly characterized, largely due to difficulties associated dissecting neuron- versus astrocyte-specific effects. Here, we use an vitro system stem cell-derived astrocytes identify profiles influenced by neurons regulate...
Neural circuit plasticity and sensory response dynamics depend on forming new synaptic connections. Despite recent advances toward understanding the consequences of plasticity, mechanisms driving are unknown. Adult-born neurons within olfactory bulb have proven to be a powerful model for studying providing broad accessible avenue into neuron development, migration, integration. We others shown that efficient adult-born integration hinges presynaptic activity in form diverse signaling...
Astrocytes are the most abundant glial cell in brain and perform a wide range of tasks that support neuronal function circuit activities. There is emerging evidence astrocytes exhibit molecular cellular heterogeneity; however, whether distinct subpopulations these diverse roles remains poorly defined. Here we show Lunatic Fringe-GFP (Lfng-GFP) bacteria artificial chromosome mouse line from both sexes specifically labels astrocyte populations within lamina III IV dorsal spinal cord....
The tumor microenvironment (TME) plays an essential role in malignancy and neurons have emerged as a key component of the TME that promotes tumorigenesis across host cancers. Recent studies on glioblastoma (GBM) highlight bi-directional signaling between tumors propagates vicious cycle proliferation, synaptic integration, brain hyperactivity; however, identity neuronal subtypes subpopulations driving this phenomenon are incompletely understood. Here we show callosal projection located...
Abstract Germline POT1 mutations are found in a spectrum of cancers and confer increased risk. Recently, we identified series novel germline that predispose carrier families to the development glioma. Despite these strong associations, how glioma-associated contribute glioma tumorigenesis remains undefined. Here show POT1-G95C increases proliferation glioma-initiating cells vitro progenitor populations developing brain. In native mouse model glioma, loss Pot1a/b resulted decreased survival...
Ionic silver (Ag+) is being investigated as a residual biocide for use in NASA spacecraft potable water systems on future crewed missions. This will be used to irrigate spaceflight crop production systems. We have evaluated the impact of three concentrations (31 ppb, 125 and 500 ppb) ionic solutions lettuce an arcillite (calcinated clay particle substrate) hydroponic (substrate-less) growth setup after 28 days. Lettuce plant was reduced samples treated with 31 ppb severely stunted at silver....
INTRODUCTION: IDH wildtype glioblastoma is the most common malignant brain tumor in adults and universally lethal. Seizures often occur as a presenting sign of tumor. Recent studies have implicated tumor-neuron communication facilitating synaptic remodeling peritumoral circuit hyperactivity influencing growth. METHODS: 63 samples were analyzed for clinical characteristics. Bulk RNA sequencing data was used to identify differentially expressed genes (DEGs), gene sets enrichment analysis...
Abstract Malignant glioma, the most lethal form of brain cancer, presents with an immunosuppressive microenvironment that obstructs tumor cell clearance and hampers immunotherapeutic interventions. Despite advancements in characterizing cellular extracellular profiles mechanisms specific to glioma remain poorly understood. We conducted single-cell RNA sequencing samples, which revealed a select subset human mouse cells express CD83, marker associated mature antigen-presenting (APCs). To...
<div>Abstract<p>Malignant glioma, the most lethal form of brain cancer, presents with an immunosuppressive microenvironment that obstructs tumor cell clearance and hampers immunotherapeutic interventions. Despite advancements in characterizing cellular extracellular profiles mechanisms specific to glioma remain poorly understood. We conducted single-cell RNA sequencing samples, which revealed a select subset human mouse cells express CD83, marker associated mature...