A5022155820- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- HIV Research and Treatment
- Galectins and Cancer Biology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Viral Infections and Vectors
- Phagocytosis and Immune Regulation
- Macrophage Migration Inhibitory Factor
- SARS-CoV-2 detection and testing
- Mosquito-borne diseases and control
- Signaling Pathways in Disease
- Complement system in diseases
- Long-Term Effects of COVID-19
- Viral Infections and Outbreaks Research
- Fire effects on ecosystems
- COVID-19 Impact on Reproduction
- COVID-19 and Mental Health
- vaccines and immunoinformatics approaches
- CAR-T cell therapy research
- Single-cell and spatial transcriptomics
- Respiratory viral infections research
- Monoclonal and Polyclonal Antibodies Research
- COVID-19 epidemiological studies
Umeå University
2016-2025
New York Academy of Sciences
2023
John Wiley & Sons (Germany)
2023
Moss Landing Marine Laboratories
2023
Hudson Institute
2023
Karolinska Institutet
2006-2017
National Institutes of Health
2008-2014
National Institute of Allergy and Infectious Diseases
2008-2014
Svenska Örtmedicinska Institute
2013
Swedish Institute
2005-2009
Understanding immune responses after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) breakthrough infection will facilitate the development of next-generation vaccines. Here, we profiled spike (S)–specific B cell Omicron/BA.1 in messenger RNA–vaccinated donors. The antibody response was characterized by high levels somatic hypermutation and a bias toward recognition ancestral SARS-CoV-2 strains, suggesting early activation vaccine-induced memory cells. BA.1 induced shift...
Heterologous prime-boost immunization strategies have the potential to augment COVID-19 vaccine efficacy. We longitudinally profiled severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S)–specific serological and memory B cell (MBC) responses in individuals who received either homologous (ChAdOx1:ChAdOx1) or heterologous (ChAdOx1:mRNA-1273) vaccination. messenger RNA (mRNA) booster induced higher serum neutralizing antibody MBC against SARS-CoV-2 variants of concern (VOCs)...
A comprehensive understanding of the development and evolution human B cell responses induced by pathogen exposure will facilitate design next-generation vaccines. Here, we utilized a high-throughput single cloning technology to longitudinally track response yellow fever virus 17D (YFV-17D) vaccine. The early memory (MBC) was mediated both classical immunoglobulin M (IgM) (IgM + CD27 ) switched (swIg MBC populations; however, IgM MBCs waned rapidly, whereas swIg atypical IgD were stable over...
To inform future preventive measures including repeated vaccinations, we have searched for a clinically useful immune correlate of protection against fatal COVID-19 among nursing homes residents.We performed capillary blood sampling with analysis S-binding IgG in an open cohort home residents Sweden. We analyzed immunological and registry data from 16 September 2021 to 31 August 2022 follow-up deaths 30 2022. The study period included implementation the 3rd 4th mRNA monovalent vaccine doses...
ObjectivesTo investigate the effectiveness of intravenous immunoglobulin (IVIG) as treatment for COVID-19 in immunocompromised patients.MethodsThis retrospective study investigated outcomes immunocompromised, vaccine non-responsive, patients that between September 2022 and April 2023 received IVIG region Västerbotten, Sweden. We analyzed clinical data, viral load, anti-SARS-CoV-2 IgG binding neutralization levels patient serum samples production batches. Primary secondary were cure...
Broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoproteins (Envs) have proven difficult to elicit by immunization. Therefore, identify effective Env neutralization targets, efforts are underway define specificities of bNAbs in chronically infected individuals. For a prophylactic vaccine, it is equally important immunogenic properties heavily glycosylated healthy primates devoid confounding HIV-induced pathogenic factors. We used rhesus macaques investigate magnitude and...
Significance In this study we investigate the origin of protective B-cell response in spleen atherosclerosis. We find an ongoing activation with production antibodies against oxidation-specific epitopes. addition, can be accelerated using apoptotic cells alone that reduce lesion development and serum cholesterol a B-cell–dependent manner. This pinpoints as important organ for atherosclerosis-associated immunity provides novel pathways to use treatment.
Humoral immunity is important in limiting clinical disease malaria, yet the longitudinal B cell response to infection remains unclear. We performed a 1-year prospective study patients treated for acute P. falciparum malaria first time, or with previous exposure disease. Using an unbiased exploratory approach mass cytometry, followed by targeted flow we found that ~80% of mature cells proliferated expressed CD11c. Only ~40% CD11c+ displayed atypical phenotype, remaining primarily made up...
Understanding the presence and durability of antibodies against SARS-CoV-2 in airways is required to provide insights into ability individuals neutralize virus locally prevent viral spread. Here, we longitudinally assessed both systemic airway immune responses upon infection a clinically well-characterized cohort 147 infected representing full spectrum COVID-19 severity, from asymptomatic fatal disease. In addition, evaluated how vaccination influenced antibody subset these during...
The rodent-borne hantavirus Puumala virus (PUUV) and related agents cause hemorrhagic fever with renal syndrome (HFRS) in humans. Other hantaviruses, including Andes (ANDV) Sin Nombre virus, a distinct zoonotic disease, cardiopulmonary (HCPS). Although these infections are severe have substantial case fatality rates, no FDA-approved countermeasures available. Recent work suggests that monoclonal antibodies may therapeutic utility. We describe here the isolation of human neutralizing (nAbs)...
The coronavirus disease 2019, COVID-19, is a complex with wide range of symptoms from asymptomatic infections to severe acute respiratory syndrome lethal outcome. Individual factors such as age, sex, and comorbidities increase the risk for infections, but other aspects, genetic variations, are also likely affect susceptibility SARS-CoV-2 infection severity. Here, we used human 3D lung cell model based on primary cells derived multiple donors identity host that regulate infection. With...
Background The long-term sequelae after COVID-19 constitute a challenge to public health and increased knowledge is needed. We investigated the prevalence of self-reported persistent symptoms reduced health-related quality life (HRQoL) in relation functional exercise capacity, 6 months infection, explored risk factors for sequalae. Methods This was prospective, multicenter, cohort study including 434 patients. At months, physical capacity assessed by 1-minute sit-to-stand test (1MSTST) were...
Abstract Background Severe COVID‐19 increases the risk for long‐term respiratory impairment, but data after mild are scarce. Our aims were to determine factors reduced function 3–6 months infection and investigate if would relate impairment of exercise performance breathlessness. Methods Patients with enrolled at University Hospitals Umeå Örebro, Karlstad Central Hospital, Sweden. Disease severity was defined as (nonhospitalized), moderate (hospitalized or without oxygen treatment), severe...
Abstract Background Large B-cell lymphoma (LBCL) is the most common and known to be a biologically heterogeneous disease regarding genetic, phenotypic, clinical features. Although prognosis good, one-third has primary refractory or relapsing which underscores importance of developing predictive biological markers capable identifying high- low-risk patients. DNA methylation (DNAm) telomere maintenance alterations are hallmarks cancer aging. Both these may contribute heterogeneity disease,...
Abstract Single-cell RNA-seq methods can be used to delineate cell types and states at unprecedented resolution but do little explain why certain genes are expressed. ATAC-seq multiome (ATAC + RNA) have emerged give a complementary view of the state. It is however unclear what additional information extracted from data besides transcription factor binding sites. Here, we show that telomere-like reads counter-inituively cannot infer telomere length, as they mostly originate subtelomere,...
The mechanisms underlying Post COVID-19 condition (PCC), with its range of long-lasting symptoms, remain unclear. This study investigates DNA methylation patterns over one year in a subset non-hospitalized patients persistent symptoms and reduced quality life, termed PCC+ (Post plus). In cohort 22 individuals matched convalescents (PCC-), we identified distinct differences between the groups that diminish time. Methylation changes TXNRD1 gene were significantly associated cognitive fatigue,...
The surface HIV-1 exterior envelope glycoprotein, gp120, binds to CD4 on the target cell induce co-receptor binding site gp120 as initial step in entry process. is comprised of a highly conserved region core, well elements third variable (V3). Antibodies against are abundantly elicited during natural infection humans, but mechanism elicitation has remained undefined. In this study, we investigate requirements for antibodies by inoculating rabbits, monkeys and human-CD4 transgenic (huCD4)...
We evaluated the performance of 11 SARS-CoV-2 antibody tests using a reference set heat-inactivated samples from 278 unexposed persons and 258 COVID-19 patients, some whom contributed serial samples. The included with variation in IgG titers, as determined by an in-house immunofluorescence assay (IFA). five rapid diagnostic had specificity 99.0% sensitivity that ranged 56.3 to 81.6% decreased low IFA titers. was > 99% for out six platform-based tests, when assessed collected ≥ 22 days after...
Pancreatic ductal adenocarcinoma (PDAC) is characterized by rich deposits of extracellular matrix (ECM), affecting the pathophysiology disease. Here, we identified galectin 4 (gal 4) as a cancer cell-produced protein that was deposited into ECM PDAC tumors and detected high-circulating levels gal in patients with PDAC. In orthotopic transplantation experiments, observed increased infiltration T cells prolonged survival immunocompetent mice transplanted reduced expression 4. Increased not...
Emerging rodent-borne hantaviruses cause severe diseases in humans with no approved vaccines or therapeutics. We recently isolated a monoclonal broadly neutralizing antibody (nAb) from Puumala virus–experienced human donor. Here, we report its structure bound to target, the Gn/Gc glycoprotein heterodimer comprising viral fusion complex. The explains broad activity of nAb: It recognizes conserved Gc loop sequences and main chain variable Gn sequences, thereby straddling locking it prefusion...
ABSTRACT Currently there is limited information about the quality of immune responses elicited by candidate human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env)-based immunogens in primates. Here we describe a comprehensive analysis neutralizing antibody and T-cell obtained cynomolgus macaques three selected immunization regimens. We used previously described YU2-based gp140 protein trimers administered an adjuvant, preceded two distinct priming strategies: either...