A5038700997- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Advancements in Transdermal Drug Delivery
- Immune Cell Function and Interaction
- Dermatology and Skin Diseases
- Immune Response and Inflammation
- Vitamin D Research Studies
- Psoriasis: Treatment and Pathogenesis
- Lipid Membrane Structure and Behavior
- Reproductive System and Pregnancy
- Influenza Virus Research Studies
- Ocular Surface and Contact Lens
- Respiratory viral infections research
- Toxin Mechanisms and Immunotoxins
- Nanoplatforms for cancer theranostics
- Advanced Drug Delivery Systems
- Food Allergy and Anaphylaxis Research
- Contact Dermatitis and Allergies
- Advanced Nanomaterials in Catalysis
- Immune cells in cancer
- Drug-Induced Adverse Reactions
- Sulfur Compounds in Biology
- Bacterial Infections and Vaccines
- Poxvirus research and outbreaks
- Transgenic Plants and Applications
Yonsei University
2017-2024
Institute of Immunology
2024
International Vaccine Institute
2008-2012
Seoul National University
2011-2012
New York University
2010
Background Vitamin D3, the most physiologically relevant form of vitamin D, is an essential organic compound that has been shown to have a crucial effect on immune responses. D3 ameliorates onset experimental autoimmune encephalomyelitis (EAE); however, direct T cells largely unknown. Methodology/Principal Findings In in vitro system using from mice, active (1,25-dihydroxyvitamin D3) suppresses both interleukin (IL)-17-producing (TH17) and regulatory (Treg) differentiation via D receptor...
Retinoic acid (RA), a well-known vitamin A metabolite, mediates inhibition of the IL-6-driven induction proinflammatory Th17 cells and promotes anti-inflammatory regulatory T cell generation in presence TGF-beta, which is mainly regulated by dendritic cells. To directly address role RA Th17/regulatory vivo, we generated A-deficient (VAD) mice continuous feeding VAD diet beginning gestation. We found that resulted significant differentiation small intestine lamina propria as early age 5 wk....
We have recently reported that the sublingual (s.l.) mucosa is an efficient site for inducing systemic and mucosal immune responses. In this study, potential of s.l. immunization to induce remote Ab responses CD8(+) cytotoxic in female genital tract was examined mice by using a nonreplicating Ag, OVA, cholera toxin (CT) as adjuvant. Sublingual administration OVA CT induced Ag-specific IgA IgG Abs blood cervicovaginal secretions. These were associated with large numbers Ab-secreting cells...
Although smallpox has been eradicated globally, the potential use of virus in bioterrorism indicates importance stockpiling vaccines. Considering advantages microneedle-based vaccination over conventional needle injections, this study, we examined feasibility as an alternative approach for We prepared polylactic acid (PLA) microneedle array patches by micromolding and loaded a second-generation vaccine on tips via dip coating. evaluated effect excipients drying conditions stability vitro...
The external part of the eye shares mucosa-associated common characteristics and is an obvious entry site for foreign Ags. We assessed potential eyedrop vaccination effective delivery vaccines against viral or bacterial infection in mice. Both OVA-specific IgG Ab serum IgA mucosal compartments were induced by eyedrops OVA with cholera toxin (CT). Eyedrop influenza A/PR/8 virus (H1N1) both virus-specific systemic responses protected mice completely respiratory virus. In addition, attenuated...
As an enveloped virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) delivers its viral genome into host cells via fusion of the and cell membranes. Here, we show that ANO6/TMEM16F-mediated surface exposure phosphatidylserine is critical for SARS-CoV-2 entry ANO6-selective inhibitors are effective against infections. Application Spike pseudotyped virus (SARS2-PsV) evokes a cytosolic Ca2+ elevation ANO6-dependent externalization in ACE2/TMPRSS2-positive mammalian cells. A...
Previous studies demonstrated cross talk between mucosal and reproductive organs during secretory IgA Ab induction. In this study, we aimed to clarify the underlying mechanisms of talk. We found significantly higher titers Ag-specific in vaginal wash after vaccination by both intranasal (i.n.) intravaginal routes but not s.c. route. Interestingly, Ab-secreting cells (ASCs) were mainly uterus cervix canal i.n. vaccination. The fact that most ASCs isolated from uteri vaccinated mice migrated...
Chitinase 3-like 1 protein (CHI3L1) (YKL-40 in humans and breast regression [BRP]-39 mice) is required for optimal allergen sensitization Th2 inflammation various chronic inflammatory diseases including asthma. However, the role of CHI3L1 airway induced by respiratory viruses has not been investigated. The aim this study was to investigate relationship between caused syncytial virus (RSV) infection. We measured YKL-40 levels human nasopharyngeal aspirate (NPA) from hospitalized children...
Abstract Topical transcutaneous immunization (TCI) presents many clinical advantages, but its underlying mechanism remains unknown. TCI induced Ag-specific IgA Ab-secreting cells expressing CCR9 and CCR10 in the small intestine a retinoic acid-dependent manner. These intestinal Abs were maintained Peyer’s patch-null mice abolished patch- lymph node-null mice. The mesenteric node (MLN) was shown to be site of isotype class switching after TCI. Unexpectedly, langerin+CD8α− dendritic emerged...
Abstract Due to the continuously mutating nature of H3N2 virus, two aspects were considered when preparing microneedle vaccines: (1) rapid preparation and (2) cross-protection against multiple antigenic variants. Previous methods measuring hemagglutinin (HA) content required standard antibody, thus vaccines targeting mutant was delayed as a result lacking antibody. In this study, prepared by high performance liquid chromatography (HPLC) without use an several variants observed. The HA...
The goal of this study is the preparation safer coated microneedles so that tips remaining after initial use are less likely to be reinserted on a second use. Twelve groups uncoated (u-MNs) were prepared from combination three different aspect ratios (height base width) and four kinds polymer (polyethylene (PE), polypropylene (PP), nylon polylactic acid (PLA)). After coating u-MNs with polyvinyl alcohol formulation make MNs (c-MNs), force displacement c-MNs was measured. ratio reduced 2.2,...
Abstract Although the mucosal and systemic immune compartments are structurally functionally independent, they engage in cross-talk under specific conditions. To investigate this cross-talk, we vaccinated mice with tetanus toxoid together cholera toxin s.c. priming followed by intrarectal (IR) boosting. Interestingly, higher numbers of Ag-specific IgA IgG Ab-secreting cells (ASCs) were detected lamina propria large intestine s.c.-IR. ASCs from colon migrated to SDF-1α/CXCL12...
Background: Vitamin D 3 , the most physiologically relevant form of vitamin D, is an essential organic compound that has been shown to have a crucial effect on immune responses.Vitamin ameliorates onset experimental autoimmune encephalomyelitis (EAE); however, direct T cells largely unknown.Methodology/Principal Findings: In in vitro system using from mice, active (1,25dihydroxyvitamin ) suppresses both interleukin (IL)-17-producing (T H 17) and regulatory (Treg) differentiation via receptor...
Abstract This study examined dendritic cells (DC) following intrarectal (IR) vaccination with the mucosal adjuvant cholera toxin (CT). Three rounds of IR ovalbumin (OVA) and CT resulted in brisk levels systemic Ig responses. Immunohistochemical studies revealed that CD11c + MHC class II accumulated primarily colonic patches (CP) lamina propria large intestine (LI‐LP), iliac LN (ILN) MLN CT. Adoptively transferred CFSE‐labeled OVA‐specific CD4 T proliferated significantly, secreting...
Background and objectives: The development of a five-in-one vaccine microneedle patch (five-in-one MN patch) aims to address challenges in administering vaccines against Diphtheria (DT), Tetanus (TT), Pertussis (wP), Hepatitis B (HBsAg), Haemophilus influenzae type b (Hib). Combining multiple into single offers novel solution improve accessibility, stability, delivery efficiency, particularly resource-limited settings. Methods: consists four distinct arrays: DT TT are coated together on one...
Triple-negative breast cancer (TNBC) represents approximately 10-17% of all cancers, and patients with TNBC show a poorer short-term prognosis than other types cancer. TNBCs also have higher tendency for early distant metastasis recurrence due to induction the epithelial-mesenchymal transition (EMT). Several recent reports suggested that inhibitor apoptosis (IAP) proteins function as regulators EMT. However, roles these in are not clear. Accordingly, we investigated cIAP2 TNBC. Among eight...
Influenza vaccine-associated anaphylaxis is a very rare allergic reaction to vaccines, but the most concerning and life-threatening adverse reaction. Although safety of influenza vaccines has been well documented, occasional cases in vaccinated patients have reported. In this study, we analyzed immunoglobulin E (IgE) response whole pediatric case delayed-onset after vaccination. The patient showed elevated specific IgE levels against especially with split virion from egg-based manufacturing...
Mycobacterium tuberculosis was responsible for 1.3 million deaths worldwide in 2022. Bacillus Calmette-Guérin (BCG) is the only licensed vaccine against (TB); however, it has limited protective efficacy adults. In this study, we constructed a recombinant vaccinia virus expressing Ag85B from M. using novel attenuated (KVAC103). We then analyzed immunogenicity of prime-boost inoculation strategies KVAC103 (rKVAC85B) compared to BCG. both rKVAC85B and BCG prime-rKVAC85B boost regimens, induced...
Although the induction mechanism of secretory IgA has been well studied, that IgG in mucosal compartments is not understood. In this study, vitamin A deficiency was convincingly shown to be associated with increased serum and intestinal fluid. We found numbers IgG-secreting B cells lamina propria small intestine mesenteric lymph node (MLN) A-deficient (VAD) mice. Of note, IFN-γ secreted by MLN dendritic (DCs) significantly augmented VAD mice, unlike control CD103(+) DCs were main subsets...
Purpose: Enzyme-linked immunosorbent assay (ELISA) has been used in the diverse field to evaluate influenza virus infection; for surveillance, diagnosis, efficacy evaluation, and development of vaccine.The aim this study was establish an ELISA detecting HA strain-specific antibodies using recombinant pandemic A H1N1 (pH1N1) HA1 (rHA1) protein.Materials Methods: rHA1 produced baculovirus system.The clinical performance developed validated human serum samples, by comparison with standard...