Patrick Reed
A5085589698- Genomic variations and chromosomal abnormalities
- Genomics and Rare Diseases
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Genetics and Neurodevelopmental Disorders
- Genomics and Chromatin Dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Renal and related cancers
- Single-cell and spatial transcriptomics
- DNA Repair Mechanisms
- Genomics and Phylogenetic Studies
- Pluripotent Stem Cells Research
- Prenatal Screening and Diagnostics
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Sleep and Wakefulness Research
- Cell Image Analysis Techniques
- interferon and immune responses
- Mathematical Biology Tumor Growth
- Hedgehog Signaling Pathway Studies
- Functional Brain Connectivity Studies
- Genetic Associations and Epidemiology
- Memory and Neural Mechanisms
- Fetal and Pediatric Neurological Disorders
- Alzheimer's disease research and treatments
Salk Institute for Biological Studies
2017-2024
University of North Carolina at Chapel Hill
2023
University of Washington
2008-2010
DNA repair within neurons Humans have only a limited capacity to generate new neurons. These cells thus need errors in the genome. To better understand this process, Reid et al. developed Repair-seq, method locate genome of stem cell–derived hotspots (DRHs) were more likely occur specific genomic features such as gene bodies well formations, open chromatin, and active regulatory regions. This showed that was enriched at sites involved neuronal function identity. Furthermore, proteomic data...
We analyzed 131 human brains (44 neurotypical, 19 with Tourette syndrome, 9 schizophrenia, and 59 autism) for somatic mutations after whole genome sequencing to a depth of more than 200×. Typically, had 20 60 detectable single-nucleotide mutations, but ~6% harbored hundreds mutations. Hypermutability was associated age damaging in genes implicated cancers and, some brains, reflected vivo clonal expansions. Somatic duplications, likely arising during development, were found ~5% normal...
Abstract Schizophrenia affects approximately 1% of the world population. Genetics, epigenetics, and environmental factors are known to play a role in this psychiatric disorder. While there is high concordance monozygotic twins, about half twin pairs discordant for schizophrenia. To address question how when twins occur, we have obtained fibroblasts from two schizophrenia (one sibling with while second one unaffected by schizophrenia) three healthy (both siblings healthy). We prepared iPSC...
Abstract When somatic cells acquire complex karyotypes, they often are removed by the immune system. Mutant that evade surveillance can lead to cancer. Neurons with karyotypes arise during neurotypical brain development, but neurons almost never origin of cancers. Instead, mutations in bring about neurodevelopmental disorders, and contribute polygenic landscape neuropsychiatric neurodegenerative disease. A subset human harbors idiosyncratic copy number variants (CNVs, “CNV neurons”),...
Schizophrenia (SZ) is a serious mental illness and neuropsychiatric brain disorder with behavioral symptoms that include hallucinations, delusions, disorganized behavior, cognitive impairment. Regulation of such behaviors requires utilization neurotransmitters released to mediate cell-cell communication which are essential functions in health disease. We hypothesized SZ may involve dysregulation secreted from neurons. To gain an understanding human SZ, induced neurons (iNs) were derived...
Abstract Somatic mosaicism is defined as an occurrence of two or more populations cells having genomic sequences differing at given loci in individual who derived from a single zygote. It characteristic multicellular organisms that plays crucial role normal development and disease. To study the nature extent somatic autism spectrum disorder, bipolar focal cortical dysplasia, schizophrenia, Tourette syndrome, multi-institutional consortium called Brain Mosaicism Network (BSMN) was formed...
As with all mathematical modeling, the scope of question to be explored determines most appropriate model. The case is no different for modeling primary brain tumors (gliomas), ranging from too simple, not accounting major feature gliomas (extensive invasion), complicated, many variables and easy way translate culture media in vitro tissue vivo. We settle on a "just right" approach which utilizes currently available magnetic resonance imaging (MRI) estimate two defining characteristics, net...
Abstract Neurons are the longest-living cells in our bodies, becoming post-mitotic early development upon terminal differentiation. Their lack of DNA replication makes them reliant on repair mechanisms to maintain genome fidelity. These decline with age, potentially giving rise genomic dysfunction that may influence cognitive and neurodegenerative diseases. Despite this challenge, knowledge how instability emerges what neurons other long-lived have evolved protect their integrity over human...