A5101425032- Redox biology and oxidative stress
- Endoplasmic Reticulum Stress and Disease
- Sirtuins and Resveratrol in Medicine
- Heat shock proteins research
- Sulfur Compounds in Biology
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- T-cell and B-cell Immunology
- Bone and Dental Protein Studies
- Oral microbiology and periodontitis research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bone Metabolism and Diseases
- Mitochondrial Function and Pathology
- RNA modifications and cancer
- Lysosomal Storage Disorders Research
- Bacterial Identification and Susceptibility Testing
- Cancer Research and Treatments
- Advanced Glycation End Products research
- Glutathione Transferases and Polymorphisms
- PARP inhibition in cancer therapy
- RNA regulation and disease
- Vibrio bacteria research studies
- Epigenetics and DNA Methylation
- Tea Polyphenols and Effects
- Magnesium in Health and Disease
Daegu-Gyeongbuk Medical Innovation Foundation
2018-2025
Northwestern University
2002-2018
Chonnam National University
2006-2016
Korea Basic Science Institute
2010
Kyungpook National University
2010
Kosin University
2010
Yonsei University Dental Hospital
2009
Kyung Hee University
2008
New York Proton Center
2007
Jeonbuk National University
2005-2006
The reduced protein expression of SIRT6 tumor suppressor is involved in tumorigenesis. molecular mechanisms underlying downregulation human cancers remain unknown. Using a proteomic approach, we have identified the ubiquitin-specific peptidase USP10, another suppressor, as one SIRT6-interacting proteins. USP10 suppresses ubiquitination to protect from proteasomal degradation. antagonizes transcriptional activity c-Myc oncogene through SIRT6, well p53, inhibit cell-cycle progression, cancer...
Mammalian 2-Cys peroxiredoxin II (Prx II) is a cellular peroxidase that eliminates endogenous H(2)O(2). The involvement of Prx in the regulation lipopolysaccharide (LPS) signaling poorly understood. In this report, we show LPS induces substantially enhanced inflammatory events, which include molecules nuclear factor kappaB and mitogen-activated protein kinase (MAPK), II-deficient macrophages. This effect was mediated by robust up-regulation reactive oxygen species (ROS)-generating...
Mito-TEMPO is a well-known mitochondria-specific superoxide scavenger. However, the effect of on porcine embryo development, to our knowledge, has not been studied yet. In present study, embryos were classified into two groups (G1 and G2) based cytoplasm lipid contents at zygote stage. The development blastocysts derived from G2 zygotes was reduced (G2:16.2 ± 7.9% vs G1: 26.5 5.9%; 1.6-fold, p < 0.05) compared those G1 zygotes. embryos, proportion TUNEL-positive cells also higher than that...
Infection with the human pathogen Vibrio vulnificus leads to generation of reactive oxygen species (ROS) via NAD(P)H oxidase (Nox) in host cells. In present study, we employed mutant V. strains identify an essential virulence factor responsible for this ROS generation. We found that repeats-in-toxin A1 (RtxA1) expressed by acts Nox1 induce significant intestine epithelial cells, which ultimately results cell death. Furthermore, RtxA1 modulates small GTPase Rac2, is known play important role...
Reactive oxygen species (ROS) actively participate in microglia-mediated pathogenesis as pro-inflammatory molecules. However, little is known about the involvement of specific antioxidants maintaining microglial oxidative balance. We demonstrate that peroxiredoxin (Prx) 5 expression up-regulated by lipopolysaccharide (LPS) through activation ROS-sensitive signaling pathway and involved attenuation both nitric oxide (NO) generation. Unlike stimulation insults with paraquat hydrogen peroxide,...
Megakaryocytic (Mk) cells mature adjacent to bone marrow (BM) sinus walls and subsequently release platelets within the sinusoidal space or in lung capillaries. In contrast, primitive stem Mk progenitor reside furthest away from BM walls. The existence of pH gradients raises question whether affects maturation differentiation. We generated peripheral blood CD34(+) a serum-free medium at different levels (7.2, 7.4, 7.6) found that higher resulted an earlier polyploidization CD41(+) onset...
We describe a case of septic shock due to Vibrio alginolyticus presenting with fever and bilateral leg pain.Despite intensive management antibiotics inotropic agents, the patient died from 1 day after hospitalization.V. was isolated both wounds blood culture.To best our knowledge, this is first reported V. bacteremia in Korea.
Mammalian peroxiredoxin V (PrdxV) is a multifunctional protein that protects cells from DNA damage and inhibits stress-induced apoptosis. However, PrdxV also known to be involved in modulating lipopolysaccharide (LPS)-induced host cell signaling, but its precise role not fully understood. In this study, we used stably transfected RAW264.7 transiently 293-mTLR4-MD2-CD14 expressing wild-type (WT) or mutant (C48S) characterize the function mechanism of action LPS-induced immune responses. We...
Peroxiredoxin V, an atypical thioredoxin peroxidase, is widely expressed in mammalian tissues. In addition, Prdx V localized mitochondria, peroxisome, cytosol, and the nucleus. has been reported to protect a wide range of cellular environments as antioxidant enzyme, its dysfunctions may be implicated several diseases, such cancer, inflammation, neurodegenerative disease. Identification relative quantification proteins affected by help identify novel signaling mechanisms that are important...
Oxidative stress promotes damage to cellular proteins, lipids, membranes and DNA, plays a key role in the development of cancer. Reactive oxygen species disrupt redox homeostasis promote tumor formation by initiating aberrant activation signaling pathways that lead tumorigenesis. We used shotgun proteomics identify proteins containing oxidation-sensitive cysteines tissue specimens from colorectal cancer patients. then compared patterns cysteine oxidation membrane fractions between non-tumor...
Bone tissue regeneration is orchestrated by the surrounding supporting tissues and involves build-up of osteogenic cells, which orchestrate remodeling/healing through expression numerous mediators signaling molecules. Periodontal models have proven useful for studying interaction communication between alveolar bone soft tissue. We applied a quantitative proteomic approach to analyze compare proteins with altered in gingival following tooth extraction. For target identification validation,...
Peroxiredoxin 5 (Prdx5) is involved in pathophysiological regulation via the stress-induced cellular response. However, its function bone remains largely unknown. Here, we show that Prdx5 osteoclast and osteoblast differentiation, resulting osteoporotic phenotypes knockout (Prdx5Ko) male mice. To investigate of bone, osteoblasts were analyzed through immunoprecipitation (IP) liquid chromatography combined with tandem mass spectrometry (LC-MS/MS) methods, while osteoclasts RNA-sequencing....
Fabry disease (FD), a lysosomal storage disorder, is caused by defective α-galactosidase (GLA) activity, which results in the accumulation of globotriaosylceramide (Gb3) endothelial cells and leads to life-threatening complications such as left ventricular hypertrophy (LVH), renal failure, stroke. Enzyme replacement therapy (ERT) Gb3 clearance; however, because short half-life body high immunogenicity FD patients, ERT has limited therapeutic effect, particularly patients with late-onset or...
Peroxiredoxin V (Prdx V) plays a major role in preventing oxidative damage as an effective antioxidant protein within variety of cells through peroxidase activity. However, the function Prdx is not limited to enzymatic activity per se. It appears have unique regulating cellular response external stimuli by directing interaction with signaling protein. In this study, we identified interacting partners mouse kidney under hypoxic stress using immunoprecipitation and shotgun proteomic analysis...
Abstract It has been reported that chitosan a hemostatic effect and an antibiotic activity. This study aimed to evaluate the efficacy feasibility of using tampon (Hemoblock-Tampon) in preventing hemorrhage enhancing wound healing after loop electrosurgical excision procedure (LEEP).This single-blind, prospective, randomized included 62 consecutive patients who underwent LEEP for cervical intraepithelial neoplasia. A (31 patients; treatment group), or general control group) was applied...
Peroxiredoxin II (Prdx II, a typical 2-Cys Prdx) has been originally isolated from erythrocytes, and its structure peroxidase activity have adequately studied. Mice lacking Prdx proteins had heinz bodies in their peripheral blood, morphologically abnormal cells were detected the dense red blood cell (RBC) fractions, which contained markedly higher levels of reactive oxygen species (ROS). In this study, labeling experiment with thiol-modifying reagent biotinylated iodoacetamide (BIAM) II-/-...
(-)-Epigallocatechin 3-gallate (EGCG) is a potent antioxidant polyphenol in green tea that acts as an anticancer agent via both direct and indirect pathways. Although the relationship between EGCG's effects its activity not fully understood, it known EGCG stimulates production of reactive oxygen species (ROS), which induce oxidative stress leading to cell death. In IM9 multiple myeloma cells, acted dose- time-dependent manner apoptotic Among enzymes expressed levels peroxiredoxin V (PrdxV)...
Small Heterodimer Partner (SHP) interacts with diverse transcription factors such as Runx2 and regulates many cellular events including differentiation, proliferation, energy metabolism. SHP is reported to be a positive regulator of BMP2-induced bone formation. This study aimed clarify the role in odontoblast differentiation matrix mineralization. Rat tooth germs were isolated, gene expression was determined by RT-PCR real-time PCR. Localization protein identified immunofluorescent analysis....