A5035705756- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Retinal Development and Disorders
- Axon Guidance and Neuronal Signaling
- Neural dynamics and brain function
- Erythrocyte Function and Pathophysiology
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Anatomy and Medical Technology
- Ion channel regulation and function
- Pluripotent Stem Cells Research
- Caveolin-1 and cellular processes
- Virus-based gene therapy research
- HIV Research and Treatment
- Nerve injury and regeneration
- Memory and Neural Mechanisms
- Surgical Simulation and Training
- Facial Trauma and Fracture Management
- Adipose Tissue and Metabolism
- Multiple Sclerosis Research Studies
- Innovations in Medical Education
- Zebrafish Biomedical Research Applications
- Cytokine Signaling Pathways and Interactions
- Dental Radiography and Imaging
Johannes Kepler University of Linz
2021-2025
University Hospital Heidelberg
2016-2025
Heidelberg University
2016-2025
Frankfurt Institute for Advanced Studies
2024
Bernstein Center for Computational Neuroscience Heidelberg-Mannheim
2020-2023
University Medical Centre Mannheim
2012-2020
Ruhr University Bochum
2003-2018
University Hospital of Basel
2013
Doheny Eye Institute
2012
University of California, Los Angeles
2012
Cortical networks are shaped by sensory experience and most susceptible to modifications during critical periods characterized enhanced plasticity at the structural functional level. A system particularly well-studied in this context is mammalian visual system. Plasticity has been documented for somatodendritic compartment of neurons detail. neuronal microdomain not yet studied axon initial segment (AIS) located proximal segment. It a specific electrogenic axonal domain site action potential...
Abstract The axon initial segment (AIS) is a critical microdomain for action potential initiation and implicated in the regulation of neuronal excitability during activity-dependent plasticity. While structural AIS plasticity has been suggested to fine-tune activity when network states change, whether it acts vivo as homeostatic regulatory mechanism behaviorally relevant contexts remains poorly understood. Using mouse whisker-to-barrel pathway model system combination with...
The microdomain that orchestrates action potential initiation in neurons is the axon initial segment (AIS). It has long been considered to be a rather homogeneous domain at very proximal hillock with relatively stable length, particularly cortical pyramidal cells. However, studies other brain regions paint different picture. In hippocampal CA1, up 50% of axons emerge from basal dendrites. Further, about 30% thick-tufted layer V rat somatosensory cortex, have dendritic origin. Consequently,...
Neurogenesis in the healthy adult murine brain is based on proliferation and integration of stem/progenitor cells thought to be restricted 2 neurogenic niches: subventricular zone dentate gyrus. Intriguingly, expressing immature neuronal marker doublecortin (DCX) polysialylated-neural cell adhesion molecule reside layer II piriform cortex. Apparently, these progressively disappear along course ageing, while their fate function remain unclear. Using DCX-CreERT2/Flox-EGFP transgenic mice, we...
Information processing in neuronal networks involves the recruitment of selected neurons into coordinated spatiotemporal activity patterns. This sparse activation results from widespread synaptic inhibition conjunction with neuron-specific excitation. We report selective hippocampal pyramidal cells patterned network activity. During ripple oscillations awake mice, spiking is much more likely which axon originates a basal dendrite rather than soma. High-resolution recordings vitro and...
Nerve growth factor (NGF) is involved in the long-term sensitization of nociceptive processing linked to chronic pain. Functional and structural ("sprouting") changes can contribute. Thus, humans report long-lasting hyperalgesia mechanical electrical stimulation after intradermal NGF injection NGF-induced sprouting has been reported underlie cancer bone pain visceral Using a human-like animal model we investigated relationship between structure function unmyelinated porcine nociceptors 3...
The axon initial segment (AIS) is essential for action potential generation. Recently, the AIS was identified as a site of neuronal plasticity. A subpopulation in cortical principal neurons contains stacks endoplasmic reticulum (ER) forming cisternal organelle (CO). function this poorly understood, but roles local Ca2+-trafficking and plasticity are discussed. To investigate whether presence and/or size COs linked to development maturation neurons, we analyzed relationship between during...
Alcohol intoxication at early ages is a risk factor for the development of addictive behavior. To uncover neuronal molecular correlates acute ethanol intoxication, we used stable-isotope–labeled mice combined with quantitative mass spectrometry to screen more than 2,000 hippocampal proteins, which 72 changed synaptic abundance up twofold after exposure. Among those were mitochondrial proteins and important morphology, including MAP6 ankyrin-G. Based on these candidate found lasting...
Multiple sclerosis (MS) is a progressive inflammatory demyelinating disease of the CNS. Increasing evidence suggests that vulnerable neurons in MS exhibit fatal metabolic exhaustion over time, phenomenon hypothesized to be caused by chronic hyperexcitability. Axonal Kv7 (outward-rectifying) and oligodendroglial Kir4.1 (inward-rectifying) potassium channels have important roles regulating neuronal excitability at around nodes Ranvier. Here, we studied spatial functional relationship between...
The extent of functional maturation and integration nonproliferative neuronal precursors, becoming neurons in the adult murine piriform cortex, is largely unexplored. We thus questioned whether precursors eventually become equivalent to neighboring principal or they represent a novel network element. Adult brain immature (complex cells) were labeled transgenic mice (DCX-DsRed DCX-CreERT2 /flox-EGFP), their cell fate was characterized with patch clamp experiments morphometric analysis axon...
The canonical view of neuronal function is that inputs are received by dendrites and somata, become integrated in the somatodendritic compartment upon reaching a sufficient threshold, generate axonal output with axons emerging from cell body. latter not necessarily case. Instead, may originate dendrites. terms ‘axon carrying dendrite’ (AcD) ‘AcD neurons’ have been coined to describe this feature. In rodent hippocampus, AcD cells shown be functionally ‘privileged’, since here can circumvent...
The axon initial segment (AIS) constitutes not only the site of action potential initiation, but also a hub for activity-dependent modulation output generation. Recent studies shedding light on AIS function used predominantly post-hoc approaches since no robust murine in vivo live reporters exist. Here, we introduce reporter line which is intrinsically labeled by an ankyrin-G-GFP fusion protein activated Cre recombinase, tagging native Ank3 gene. Using confocal, superresolution, and...
Anatomical embalming forms a mainstay in the provision of teaching materials based on human post-mortem tissues. As consequence regulations worldwide to minimize use formaldehyde due its harmful nature, effective measures must be taken application anatomy laboratory. This study aimed identify commonalities among protocols used Austrian, German and Swiss anatomies reduce usage provide foundation for joint accreditation. Survey data all procedures, technologies chemicals related were collected...
Cardiac pressure overload (for example due to aortic stenosis) induces irreversible myocardial dysfunction, cardiomyocyte hypertrophy and interstitial fibrosis in patients. In contrast adult, neonatal mice can efficiently regenerate the heart after injury first week birth. To decipher whether insufficient cardiac regeneration contributes progression of dependent disease, we established a transverse constriction protocol (nTAC). nTAC non-regenerative stage (at postnatal day P7) induced...
Abstract Regional anatomy teaching forms a cornerstone of undergraduate medical education. Owing to an increase in and learning content throughout the curriculum recent years, contact hours overall course durations are under review worldwide. This study aimed assess whether shortening duration impacts gain ability identify anatomical structures correctly. Undergraduate students Johannes Kepler University Linz (JKU; n = 310) at Medical Graz (MUG; 156) participating regional courses were...
Abstract The axon initial segment (AIS) constitutes not only the site of action potential initiation, but also a hub for activity-dependent modulation output generation. Recent studies shedding light on AIS function used predominantly post-hoc approaches since no robust murine in vivo live reporters exist. Here, we introduce reporter line which is intrinsically labeled by an ankyrin-G-GFP fusion protein activated Cre recombinase, tagging native Ank3 gene. Using confocal, superresolution, and...