Inducing immune tolerance through repeated administration of self-antigens is a promising strategy for treating rheumatoid arthritis (RA), and current research indicates that coadministration immunomodulators can further orchestrate the tolerogenic response. However, most clinical trials based on induction have negligible therapeutic effects. Peripheral lymphoid organs play critical roles in immunotherapy. Here, we design an engineered nanoemulsion targeted codelivery immunomodulator to...

Abstract Background Ligamentum flavum hypertrophy (LFH) is a primary contributor to lumbar spinal stenosis. However, thorough understanding of the cellular and molecular mechanisms driving LFH fibrotic progression remains incomplete. Methods Single-cell RNA sequencing (scRNA-seq) was performed construct single-cell map human ligamentum (LF) samples. An integrated multi-omics approach, encompassing scRNA-seq, bulk (bulk RNA-seq), Mendelian randomization (MR), applied conduct comprehensive...

Mechanical stress is an important risk factor for intervertebral disc degeneration (IVDD). Angiopoietin-2 (ANG-2) regulated by mechanical and widely involved in the regulation of extracellular matrix metabolism. In addition, signaling cascade between HIF-1α NF-κB critical degradation. This study aims to investigate role molecular mechanism ANG-2 regulating annulus fibrosus (AF) through HIF-1α/NF-κB pathway.The bipedal standing mice IVDD model was constructed, histological experiments were...

To test the role of STAT3 in human rhabdomyosarcoma cells, genetic approaches were used to either knockdown expression and GP130, an upstream activator using short hairpin RNA (shRNA) or express persistently active protein. Knockdown GP130 sensitized cells anti-cancer drugs doxorubicin, cisplatin, MEK inhibitor AZD6244. On other hand, constitutively protein reduced sensitivity those drugs. In addition, we tested a small molecule LY5 bazedoxifene cells. Our data demonstrated that combination...

Abstract Ligamentum flavum hypertrophy (LFH) is a major cause of lumbar spinal canal stenosis (LSCS). The pathomechanisms for LFH have not been fully elucidated. Isobaric tags relative and absolute quantitation (iTRAQ) technology, proteomics assessments human ligamentum (LF), successive assays were performed to explore the effect clusterin (CLU) upregulation on pathogenesis. samples exhibited higher cell positive rates CLU, TGF-β1, α-SMA, ALK5 p-SMAD3 proteins than non-LFH samples....

Abstract We discovered a thalidomide analogue [5-hydroxy-(2,6-diisopropylphenyl)-1H-isoindole-1,3-dione (5HPP-33)] with antiproliferative activity against nine cancer cell lines in vitro. Flow cytometric analyses showed that the compound caused G2-M arrest, which occurred mainly at mitotic phase. In addition, immunofluorescence microscopy and vitro tubulin polymerization studies 5HPP-33 has antimicrotubule paclitaxel-like mode of action. It is effective four different paclitaxel-resistant...

In order to closely mimic a multi-cell state in hematopoietic stem/progenitor cells (HSC/HPCs) vascular niche, we co-cultured human bone marrow mesenchymal stem (hBMSCs) and umbilical vein endothelial (HUVECs) without any cytokines as feeder applied bio-derived from femoral metaphyseal portion scaffold develop new HSC/HPCs three-dimensional culture system (named 3D-Mix cultures). Scanning electron fluorescent microscopy showed excellent biocompatibility of hBMSCs HUVECs vitro. Flow cytometry...

Novel star-shaped poly(ϵ-caprolactone)-b-poly(2-hydroxyethyl methacrylate) (sPCL-b-PHEMA) with 3 arm and 6 was synthesized by a combination of ring-opening polymerization atom transfer radical polymerization. The structure copolymers confirmed nuclear magnetic resonance (1H 13C NMR) Fourier transform infrared spectroscopy. thermal behavior measured differential scanning calorimetry. results showed that Tc, Tm, Xc the sPCL-b-PHEMA were reduced along increase in length PHEMA block. could...

Purposes of the study To evaluate benefit stenting iliac vein in patients with residual stenosis treated catheter-directed thrombolysis for acute iliofemoral deep venous thrombosis. Procedures In this randomized prospective study, a first-time lower extremity thrombosis that had persisted <14 days were thrombolysis. After thrombolysis, >50% randomly divided into two groups: + Stent Group and Alone Group. Patients received urokinase low-molecular-weight heparin/oral warfarin during...

Ligamentum flavum hypertrophy (LFH) leads to lumbar spinal stenosis (LSS) caused by LF tissue inflammation and fibrosis. Emerging evidence has indicated that dysregulated microRNAs (miRNAs) have an important role in Mechanical stress (MS) been explored as initiating step LFH pathology progression; the inflammation-related miRNAs induced after mechanical implicated fibrosis pathology. However, pathophysiological mechanism of MS-miRNAs-LFH remains be elucidated. Using sequencing analysis...

Background Brainstem hemorrhage has a rapid onset with high mortality and disability rates. In recent years, an increasing number of studies have reported on the surgical treatment brainstem hemorrhage. The introduction stereotaxic instruments navigation systems improved accuracy treatment; however, popularity these devices in primary hospitals is not high. this study, we introduce laser combined XperCT technology to assist puncture drainage hemorrhage, aiming improve facilitate hospitals....

Catheter-directed thrombolysis (CDT) has been a mainstay in treating deep venous thrombosis (DVT). However, the optimal dosage of thrombolytic agent is still controversial. The goal this study was to evaluate safety and efficacy low urokinase with CDT for DVT.A retrospective analysis performed using data from total 427 patients DVT treated our single center between July 2009 December 2012. Early assessed thrombus score based on daily venography. therapeutic evaluated by adverse events. A...

Rheumatoid arthritis (RA) is a common autoimmune and inflammatory disease. Activated macrophages in arthritic joints play prominent role the initiation persistence of RA. Despite great progress clinical treatment RA, poor response high discontinuation due to systemic toxicity remain unsolved issues, especially well-known methotrexate (MTX). Therefore, active targeted delivery therapeutic drugs pathogenic cells essential increase situ activity decrease toxicity. Here, we developed an...

Background Hypertensive intracerebral hemorrhage (HICH) is a severe life-threatening disease, and its incidence has gradually increased in recent years. Due to the particularity diversity of bleeding sites, early treatment hematoma needs be more meticulous accurate, minimally invasive surgery often one measures that are commonly adopted now. The lower debridement navigation template created by 3D printing technology were compared external drainage hypertensive cerebral hemorrhage. Then...

Lung cancer is the most lethal malignancies with high aggressive and poor prognosis. Until now, five-year survival rate has not been improved which brings serious challenge to human health. stem cells (LCSCs) serve as root of occurrence, progression, recurrence, drug resistance. Therefore, effective anti-cancer agents molecular mechanisms could specifically eliminate LCSCs are urgently needed for design. In this article, we discovered Olig2 was overexpressed in clinical lung tissues acted a...

Our objective was to study the clinical feasibility of Xper-CT combined with laser-assisted radiofrequency thermocoagulation in treatment trigeminal neuralgia.A retrospective analysis made 60 patients neuralgia who visited Affiliated Hospital Binzhou Medical University from January 2019 May 2021. According different surgical methods, we were divided into C-arm X-ray group and laser navigation group. The operation time, operative complications, post-operative 24 h, 3 6 months Barrow Neurotics...