To evaluate the safety profile and efficacy of bone marrow mononuclear cells (BMMNC) transplantation for ST-segment elevation myocardial infarction (STEMI) by assessing patients their left ventricular function at up to 4 years follow-up. Eighty-six with STEMI who had successfully undergone percutaneous coronary intervention (PCI) were randomized receive intracoronary injection BMMNC (n = 41) or saline 45). Left ejection fraction, as evaluated UCG, was markedly improved 6 months (0.484 ± 0.5...

Macrophages are a heterogeneous group of phagocytes that play critical roles in inflammation, infection and tumor growth. respond to different environmental factors thereby polarized into specialized functional subsets. Although hypoxia is an important factor, its impact on human macrophage polarization subsequent modification the inflammatory microenvironment have not been fully established. The present study aimed elucidate effect exposure ability macrophages polarize classically activated...

To explore the METTL14-dependent m6A modification mechanism involved in development of atherosclerosis. Oxidized low-density lipoprotein (ox-LDL) and HUVEC cell line were used to establish an atherosclerosis model vitro, APOE-/- mice fed a high-fat diet as animal model. Cell viability apoptosis assessed using MTT assays flow cytometry. The status HUVECs was examined MeRIP-qPCR. Oil Red O staining evaluate lesions or plaques on aortas separated from target mice. METTL14 METTL3 upregulated...

Hypoxia/reoxygenation (H/R) is one of the main causes coronary artery disease (CAD), which primarily induced by damage to endothelial cells (CAECs). Glycyrrhizic acid (GA) a natural and abundant pentacyclic triterpenoid glycoside licorice root extract, it has been reported elicit protective effects against hypoxia, inflammation apoptosis in ischemic myocardium; therefore, GA may serve as promising therapeutic agent for ischemia-associated CAD. In present study, H/R-induced injury CAECs were...

Abstract Aim: Circulating microRNA expression has become a biomarker of cardiovascular disease; however, the association between circulation and myocardium in hypertrophic cardiomyopathy remains unclear. The present study aimed to find circulating correlated not only myocardial expression, but also cardiac hypertrophy fibrosis. Method: Forty-two cases obstructive (HOCM) diagnosed by echocardiography magnetic resonance were analysed for plasma tissue. Results: results showed that miR-221 was...

Impaired glucose metabolism is implicated in cardiac failure during ischemia-reperfusion. This study examined uptake and expression of transport-4 (GLUT-4) dogs undergoing Cardiac ischemia was induced by cardiopulmonary bypass for 30 min or 120 dogs. Plasma insulin concentrations were measured at pre-bypass (control), aortic cross-clamp off (ischemia-reperfusion) 15, 45, 75 min. At the same time, left ventricle biopsies taken GLUT-4 immunohistochemistry glycogen content analysis. In...

BACKGROUND:The aim of this study was to determine the role AMP-activated protein kinase (AMPK) in myocardial insulin resistance after ischemia-reperfusion during cardiopulmonary bypass surgery dogs. MATERIAL AND METHODS:Twenty-four mongrel dogs were randomly assigned 4 groups. The control group did not undergo aortic cross-clamping; model underwent 60 mins cross-clamping with 150 ml cardioplegic solution. treatment group, inhibition respectively 0.11mg/kg AICAR (AMPK agonist) solution and...

Abstract The present study investigated the effects of N-acetylcysteine (NAC) supplementation on rectal temperature, respiratory rate, growth performance and redox status in broilers, over a 21-day period at an elevated environmental temperature. A total 270 one-day-old Arbor Acres broiler chickens were randomly allocated into three groups with five replicates 18 broilers per replicate, viz. thermoneutral group, which was reared standard ambient temperature fed basal diet (CONT); two...

Abstract Vein graft failure after coronary artery bypass grafting (CABG) is primarily caused by intimal hyperplasia, which results from the phenotypic switching of venous smooth muscle cells (SMCs). This study investigates role and underlying mechanism miR-16-5p in SMCs. In rats, neointimal thickness area increased over time within 28 days CABG, as did time-dependent downregulation SMC switching. Platelet-derived growth factor-BB-induced HSVSMCs was accompanied substantially linked with...

Doxorubicin (DOX) has been widely employed to treat cancer, particularly solid tumors and hematological malignancies, owing its high efficacy; however, chemotherapy indicated be cardiotoxic induce adverse effects, including mitochondrial dysfunction DNA damage, which limits application. The mitochondria‑associated protein leucine‑rich pentatricopeptide repeat‑containing (LRPPRC) reported serve critical regulatory roles in physiological processes via regulating function. aim of the present...

Abstract Background Carney syndrome is an uncommon autosomal disorder closely linked to mutations in the PRKAR1A gene. Skin lesions are most pronounced feature of syndrome, affecting over 80% individuals with this condition. This characterized by a triad myxomas, skin pigmentation, and endocrine hyperfunction, featuring multiple neoplasms cardiac involvement. Dilated cardiomyopathy, primary defined as dilation impaired systolic function left or both ventricles. Its clinical presentation...

To screen Myocardial ischemia-reperfusion Injury in mice. adenosine monophate-activatedprotein kinase (AMPK) -related differentially expressed circularRNA (circRNA) MIRI model, Ampk-related circRNA network was drawn to provide possible ideas for the prevention and treatment of MIRI.

Abstract Background Pericardial calcification is usually a marker of chronic diseases, and its occurrence in rapidly progressing malignant primary pericardial mesothelioma (PPM) extremely rare. Therefore, this atypical imaging appearance contributes to more frequent misdiagnosis PPM. However, no systematic summary currently exists the characteristics In our report, clinical are discussed detail, provide reference reduce rates Case presentation A 50-year-old female patient was admitted...

Background: To retrospectively study the experience with application of no-touch technique in radial artery (RA)-based coronary bypass grafting (CABG). Methods: Clinical data patients who underwent RA-based multi- (n = 45) or full-arterial CABG 27) between January 2019 and June 2022 Affiliated Hospital ZunYi Medical University were collected. The incidence main cardiovascular events at 30-day follow-up, antebrachial union condition vessel patency rate analyzed. Results: A total 66 RAs...

: Aortic dissection (AD) is a cardiovascular emergency that seriously endangers human health. It has acute onset, dangerous condition and many complications. The mortality without treatment very high, the within 24 hours 25%. AD combined with mesenteric artery malperfusion an incidence of only 5%, but hospital rate up to 33–100%. Mesenteric increases by 3–4 times. Even after complete revascularization, ischemia/reperfusion injury still leads frequent postoperative deaths. In this paper, we...

Objective: To evaluate the mid-term outcomes of coronary artery bypass grafting (CABG) with left radial (RA) graft bypassed to right main (RCA) severe stenosis. Methods: Between September 2014 and April 2019, a total consecutive 47 patients who had stenosis (≥90%) RCA underwent arterial revascularization, RA RCA. There were 31 males 16 females, mean age (56.5±9.7) years old. The perioperative observed results followed up. Results: A 46 internal mammary (LIMA) grafts, (LRA), 40 grafts (RRA)...

Marfan syndrome (MFS) is a connective tissue disorder involving multiple organs. The most severe complications include aortic root dilatation and dissection. In the present report, we provide an uncommon case of acute Stanford type A dissection (AADA) repair with scoliosis in MFS patient it even more rare for such surgical treatment to be successfully completed along holistic management that enables recover successfully. We offer reference future therapy since specific methods this have not...

More and more evidence indicates the central role of CD8 T cells in allo immune responses. They are responsible for resistance to tolerance many circumstances. To define exact function population allograft rejection tolerance, we analyzed cytokine production profile both CD4 cells. In wild-type mice, IFN-Υ IL-6 two major cytokines (1865±616.7 565.5±100.3 pg/ml respectively) that observed be up-regulated at time rejection. None other (IL-1a, IL-1b, IL-2, IL-4, IL-5, IL-9, IL-12p40, IL-12p70,...

Objective To observe interference RNAi silencing of adult rat cardiomyocytes hypoxia- inducible factor- 1 alpha(HIF- 1α)of the experimental possibilities and transfection efficiency. Methods Construction small interfering RNA(siRNA)targeting HIF- 1αgenes transfected into cells rats.Reverse transcriptase polymerase chain reaction(RT- PCR)testing 1αmRNA levels in cardiac myocytes,methyl thiazol tetrazolium(MTT)method for detection myocardial rats different concentrations cells, with...

Objective To investigate the expression of phosphatidylinositol 3 kinase (PI3K) in process myocardial insulin resistance induced by extracorporal circulation ischemia repeffusion,making clear whether cells induce transfer glucose transporter protein-4 (Glut-4) way PI3K,and further probing into mechanism post-receptor course cardiopulmonary bypass (CPB) ischemia.Methods Twenty-four mongrel dogs were randomly divided 4 groups:group Ⅰ undergoing 30-min aortic occlusion; group Ⅱ 120 min Ⅲ 30...