A5019460834- Spine and Intervertebral Disc Pathology
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- Musculoskeletal pain and rehabilitation
- Osteoarthritis Treatment and Mechanisms
- Cervical and Thoracic Myelopathy
- Electrospun Nanofibers in Biomedical Applications
- Orthopaedic implants and arthroplasty
- Spinal Fractures and Fixation Techniques
- Bone and Joint Diseases
- Inflammatory mediators and NSAID effects
- Magnesium Alloys: Properties and Applications
- Spinal Cord Injury Research
- Silk-based biomaterials and applications
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- Wound Healing and Treatments
- Periodontal Regeneration and Treatments
- Neonatal Respiratory Health Research
- Cancer-related molecular mechanisms research
- Medical Imaging and Analysis
- Proteoglycans and glycosaminoglycans research
- Wnt/β-catenin signaling in development and cancer
- Intraoperative Neuromonitoring and Anesthetic Effects
Peking University Shenzhen Hospital
2016-2025
Hong Kong University of Science and Technology
2022-2025
University of Hong Kong - Shenzhen Hospital
2022-2025
University of Hong Kong
2012-2023
University of Cincinnati
2019
Orthopaedic Research
2018
Queen Mary Hospital
2013-2017
Chinese University of Hong Kong
2011-2017
Taoyuan Chang Gung Memorial Hospital
2014
Sichuan University
2008-2012
The regeneration of critical-size bone defects, especially those with irregular shapes, remains a clinical challenge. Various biomaterials have been developed to enhance regeneration, but the limitations on shape-adaptive capacity, complexity operation, and unsatisfied osteogenic bioactivity greatly restricted their application. In this work, we construct mechanically robust, tailorable water-responsive shape-memory silk fibroin/magnesium (SF/MgO) composite scaffold, which is able quickly...
Abstract Fibrotic remodeling of nucleus pulposus (NP) leads to structural and mechanical anomalies intervertebral discs that prone degeneration, leading low back pain incidence disability. Emergence fibroblastic cells in disc degeneration has been reported, yet their nature origin remain elusive. In this study, we performed an integrative analysis multiple single-cell RNA sequencing datasets interrogate the cellular heterogeneity fibroblast-like entities degenerative human NP specimens. We...
Ligamentum flavum (LF) hypertrophy (LFH) has been recognised as one of the key contributors to lumbar spinal stenosis. Currently, no effective methods are available ameliorate this hypertrophy. In study, human umbilical cord mesenchymal stromal cell-derived extracellular vesicles (hUCMSC-EVs) were introduced for first time promising vehicles drug delivery treat LFH. The downregulation miR-146a-5p and miR-221-3p expressions in LF tissues negatively correlated with increased thickness....
The healing of critical-sized bone defects (CSD) remains a challenge in orthopedic medicine. In recent years, scaffolds with sophisticated microstructures fabricated by the emerging three-dimensional (3D) printing technology have lighted up treatment CSD due to elaborate microenvironments and support they may build. Here, we established magnesium oxide-reinforced 3D-printed biocomposite scaffold investigate effect magnesium-enriched 3D microenvironment on repairing. composite was prepared...
Human multipotent stem cell-based therapies have shown remarkable potential in regenerative medicine and tissue engineering applications due to their abilities of self-renewal differentiation into multiple adult cell types under appropriate conditions. Presently, human cells can be isolated from different sources, but variation among basic biology result suboptimal selection seed preclinical clinical research. Thus, the goal this study was compare biological characteristics bone marrow,...
Abstract Type 2 diabetes (T2D) is associated with systemic abnormal bone remodeling and loss. Meanwhile, subchondral induces cartilage degradation, resulting in osteoarthritis (OA). Accordingly, we investigated alterations remodeling, microstructure strength knees from T2D patients their association degradation. Tibial plateaus were collected knee OA undergoing total arthroplasty divided into non-diabetic ( n =70) =51) groups. also cadaver donors =20) used as controls. Subchondral was...
Magnesium (Mg) alloys, having a unique combination of strength and degradation, are being explored for various craniofacial orthopedic applications.
Intervertebral disc degeneration (IDD) is associated with low back pain, a leading cause of disability worldwide. Fibrosis nucleus pulposus (NP) principal component IDD, featuring an accumulation myofibroblast-like cells. Previous study indicated matrix metalloproteinase 12 (MMP12) expression upregulated in IDD but its role remains largely unexplored. We here showed that TGF-β1 could promote differentiation human NP cells along induction MMP12 expression. Intriguingly, knockdown not only...
The combination of osteogenesis and angiogenesis dual-delivery trace element-carrying bioactive scaffolds stem cells is a promising method for bone regeneration repair. Canonical Wnt HIF-1α signaling pathways are vital BMSCs’ osteogenic differentiation secretion factors, respectively. Simultaneously, lithium (Li) copper (Cu) can activate the canonical pathway, Moreover, emerging evidence has shown that HIF related to coupling angiogenesis. However, it still unclear whether lithium-...
Bone marrow-derived mesenchymal stem cells (BM-MSCs) are the popular seed for regenerative medicine, and there has been a rapid increase in number of BM-MSC-based clinical trials. However, safety these should also be closely studied. In this study, spontaneous calcification BM-MSCs from rats was evaluated normoxia (20% O(2)) without osteogenic medium after continuous culture 21 days; obvious mineralized nodules were observed, which positive Alizarin Red, collagen-I (Col-I), osteocalcin (OC)...
Abstract MSCs (mesenchymal stem cells) may be promising seed cells for tissue regeneration because of their self‐renewal and multi‐differentiation potential. Shh (sonic hedgehog) is involved in the skeletal formation during embryo development regeneration. However, how regulates biological characteristics BM‐MSCs (bone marrow‐derived MSCs) poorly understood. We have investigated effect rShh‐N (recombinant N‐terminal Shh) on proliferation osteogenic differentiation rBM‐MSCs (rat BM‐MSCs)...