A5075207773- Adipose Tissue and Metabolism
- Diet and metabolism studies
- Glioma Diagnosis and Treatment
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Eicosanoids and Hypertension Pharmacology
- Hormonal Regulation and Hypertension
- Tuberous Sclerosis Complex Research
- TGF-β signaling in diseases
- Ion channel regulation and function
- Neural dynamics and brain function
- Traumatic Brain Injury and Neurovascular Disturbances
- Extracellular vesicles in disease
- Neuroscience and Neural Engineering
- Nitric Oxide and Endothelin Effects
- Drug Transport and Resistance Mechanisms
- Intracerebral and Subarachnoid Hemorrhage Research
- RNA regulation and disease
- Piperaceae Chemical and Biological Studies
- interferon and immune responses
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- RNA Research and Splicing
- Cellular transport and secretion
- Immune Cell Function and Interaction
- Neurogenesis and neuroplasticity mechanisms
Columbia University
2008-2025
Neurological Surgery
2008-2025
Nanchang University
2006-2025
First Affiliated Hospital of Nanchang University
2025
Wenzhou Medical University
2025
Columbia University Irving Medical Center
2015-2024
Xian Central Hospital
2018-2024
Xi'an Jiaotong University
2024
University of Washington
2022
Sichuan Normal University
2018-2021
To examine the diversity of astrocytes in human brain, we immunostained surgical specimens temporal cortex and hippocampus autopsy brains for CD44, a plasma membrane protein extracellular matrix receptor. CD44 antibodies outline details astrocyte morphology to degree not possible with glial fibrillary acidic (GFAP) antibodies. CD44+ could be subdivided into two groups. First, long processes were consistently found subpial area ("interlaminar" astrocytes), deep isocortical layers,...
Normal axonal mitochondrial transport and function is essential for the maintenance of synaptic function. Abnormal motility dysfunction within axons are critical amyloid β (Aβ)-induced stress loss synapses relevant to pathogenesis Alzheimer's disease (AD). However, mechanisms controlling alterations in AD remain elusive. Here, we report an unexplored role cyclophilin D (CypD)-dependent permeability transition pore (mPTP) Aβ-impaired trafficking. Depletion CypD significantly protects dynamics...
Preeclampsia is a pregnancy-induced condition that impairs the mother's health and results in pregnancy termination or premature delivery. Elevated levels of placenta-derived extracellular vesicles (pcEV) circulation have been consistently associated with preeclampsia, but whether these induce preeclampsia are product not known. Guided by small cohort study preeclamptic patients, we examined impact pcEV on pathogenesis mouse models. We detected pregnant C56BL/6J mice peak level...
Summary Purpose: Cortical tubers are epileptogenic lesions in patients with tuberous sclerosis complex (TSC). Giant cells and dysplastic neurons pathological hallmarks of cortical tubers. Severe astrogliosis, which is invariably present tubers, has attracted much less attention. We hypothesize that the development astrogliosis constitutes a primary pathology astrocytes directly related to TSC 1/2 mutations.. Methods: To begin test this hypothesis, we performed immunohistochemical electron...
Abstract Multidrug resistance-associated protein 3 (MRP3, ABC subfamily C [ABCC]3) plays an important role in protecting hepatocytes and other tissues by excreting array of toxic organic anion conjugates, including bile salts. MRP3/ABCC3 expression is increased the liver some cholestatic patients, but molecular mechanism this up-regulation remains elusive. In report, we assessed patients (n = 22) with obstructive cholestasis caused gallstone blockage ducts noncholestatic patient controls...
Alexander Disease (AxD) is a primary disorder of astrocytes, caused by heterozygous mutations in GFAP , which encodes the major astrocyte intermediate filament protein, glial fibrillary acidic protein (GFAP). Astrocytes AxD display hypertrophy, massive increases GFAP, and accumulation Rosenthal fibers, cytoplasmic inclusions containing small heat shock proteins. To study effects on morphology physiology, we have examined hippocampal astrocytes three mouse models AxD, transgenic line ( Tg )...
Alexander disease (AxD) is a leukodystrophy caused by heterozygous mutations in the gene for glial fibrillary acidic protein, an intermediate filament protein expressed astrocytes. The mutation causes prominent aggregates inside astrocytes; there also loss of myelin and oligodendrocytes neuronal degeneration. We show that immunohistochemical staining glutamate transporter 1, major brain primarily astrocytes suggests decreased levels hippocampi infantile AxD patients. A knock-in mouse model...
Mammalian target of rapamycin (mTOR) is a key protein kinase that regulates basic cellular processes, including development and growth. Mutations in mTOR cause tuberous sclerosis complex (TSC), condition characterized by developmental brain malformations (cortical tubers) epilepsy. Although considerable insight has been gained recently into the pathologic dysfunction tubers TSC-related epilepsy, data on cascade mesial temporal lobe epilepsy (MTLE) are lacking. Immunohistochemical...
Highlights•Tsc1-null late embryonic RGCs generate cytomegalic pyramidal neurons (CPNs)•CPNs are synaptically hyperexcitable and susceptible to seizure-like activities•Enhanced synaptic excitation in CPNs contributes epileptogenesis•Astrocyte gliosis evolves secondary recurrent seizuresSummaryTuberous sclerosis complex (TSC) is a developmental disorder associated with epilepsy, autism, cognitive impairment. Despite inactivating mutations the TSC1 or TSC2 genes hyperactive mechanistic target...
Like many other cell surface receptors, transforming growth factor beta (TGF-beta) receptors are internalized upon ligand stimulation. Given that the signaling-facilitating molecules Smad anchor for receptor activation (SARA) and Hrs mainly localized in early endosomes, it was unclear whether internalization is required Smad2 activation. Using reversible biotin labeling, we directly monitored of TGF-beta type I receptor. Our data indicate endocytosed via a clathrin-dependent mechanism...
The identification of highly potent and orally bioavailable GPR39 agonists is reported. Compound 1, found in a phenotypic screening campaign, was transformed into compound 2 with good activity on both the rat human receptor. This further optimized to improve ligand efficiency pharmacokinetic properties yield for potential oral treatment type diabetes. Thus, 3 first agonist (EC50s ≤ 1 nM receptor) that mice robustly induced acute GLP-1 levels.
Coagulopathy often develops soon after acute traumatic brain injury and its cause remains poorly understood. We have shown that injured brains release cellular microvesicles disrupt the endothelial barrier induce consumptive coagulopathy. Morphologically intact extracellular mitochondria accounted for 55.2% of these microvesicles, leading to hypothesis are metabolically active serve as a source oxidative stress activates platelets renders them procoagulant. In testing this experimentally, we...
Objective: To explore the value of immature platelets and thromboelastography (TEG) in evaluating bleeding tendency patients bitten by blood venomous snakes. Methods: In December 2023, a scoring system was used to grade score degree 147 with snake bites. Immature TEG were also tested analyze correlation between indicators such as TEG, platelet ratio (IPF), absolute (A-IPF). The measurement data represented Mean±SD, ANOVA for comparison among groups. Statistical analyzed using χ(2) test....
Recent evidence has implicated perituberal, MRI-normal brain tissue as a possible source of seizures in tuberous sclerosis complex (TSC). Data on aberrant structural features this area that may predispose to the initiation or progression are very limited. We used immunohistochemistry and confocal microscopy compare epileptogenic, with cortical tubers.In every sample perituberal tissue, we found many abnormal cell types, including giant cells cytomegalic neurons. The majority were surrounded...
The differentiation of memory CD8+ T cells is critical to the long-term cellular immunity. transcription factor BCL6 has been reportedly important for generation and maintenance cells; however, using newly established conditional knockout mouse model, we demonstrate that dispensable cell pool, although still required precursors upon acute viral infection. In addition, promotes expression TCF-1 via directly binding Tcf7 (gene symbol TCF-1) allele in forced restores BCL6-deficient precursors....
High-grade gliomas (HGG) are deadly diseases for both adult and pediatric patients. Recently, it has been shown that neuronal activity promotes the progression of multiple subgroups HGG. However, epigenetic mechanisms govern this process remain elusive. Here we report chromatin remodeler chromodomain helicase DNA-binding protein 2 (CHD2) regulates neuron-glioma interactions in diffuse midline glioma (DMG) characterized by onco-histone H3.1K27M. Depletion CHD2 H3.1K27M DMG cells compromises...
Hemorrhagic stroke is a devastating cerebrovascular event with high rate of early mortality and long-term disability. The therapeutic potential mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) for neurological conditions, such as intracerebral hemorrhage (ICH), has garnered considerable interest, though their mechanisms action remain poorly understood.
Dynamin 3 (DNM3) is a member of family motor proteins that participate in number membrane rearrangements such as cytokinesis, budding transport vesicles, phagocytosis, and cell motility. Recently, DNM3 was implicated having role megakaryocyte (MK) development. To further investigate the functional during megakaryocytopoiesis, we introduced sequence-specific short hairpin RNAs (shRNAs) into developing MKs. The results showed knockdown inhibited stage MK development involved progenitor...