A5019248582- Fatty Acid Research and Health
- Sesame and Sesamin Research
- Diet and metabolism studies
- Nutritional Studies and Diet
- Eicosanoids and Hypertension Pharmacology
- Adipose Tissue and Metabolism
- Peptidase Inhibition and Analysis
- Gestational Trophoblastic Disease Studies
- Nutrition and Health in Aging
- Lower Extremity Biomechanics and Pathologies
- Cholesterol and Lipid Metabolism
- Ginger and Zingiberaceae research
- Fungal and yeast genetics research
- Antioxidant Activity and Oxidative Stress
- Lipid metabolism and biosynthesis
- Muscle Physiology and Disorders
- Body Composition Measurement Techniques
- Epigenetics and DNA Methylation
- Adenosine and Purinergic Signaling
- Signaling Pathways in Disease
- Ginkgo biloba and Cashew Applications
- Muscle activation and electromyography studies
- Telomeres, Telomerase, and Senescence
- Antiplatelet Therapy and Cardiovascular Diseases
- Cynara cardunculus studies
Suntory Foundation for Life Sciences
2003-2024
Suntory (Japan)
1996-2020
Kyoto Prefectural University of Medicine
1997
Kyoto University
1994-1995
The serum level of placental leucine aminopeptidase (P-LAP) increases during pregnancy. P-LAP degrades several peptide hormones such as oxytocin and vasopresin, suggesting a role in maintaining homeostasis In the study reported here, we have isolated cDNA clone with 4084 base pairs encoding from human library. amino acid sequence deduced contained all sequences fragments obtained by digestion purified protein trypsin. predicted contains HEXXH consensus zinc metallopeptidases, indicating that...
Serum levels of human placental leucine aminopeptidase/oxytocinase (P-LAP) increase with gestation. cDNA cloning P-LAP revealed that the enzyme is a type II membrane-bound protein containing consensus HEXXH(X)18E motif found in M1 family zinc-metallopeptidase proteins. In this study, recombinant soluble form maternal serum was expressed Chinese hamster ovary cells, purified to homogeneity and then characterized. Although N-terminal sequencing four-amino-acid deletion, active shown be...
The placental leucine aminopeptidase (P‐LAP)/oxytocinase whose serum level increases with gestation is thought to contribute the maintenance of normal pregnancy. P‐LAP mRNAs are expressed in various tissues other than placenta. In this study, we identified protein brain. contrast placenta where a significant portion released, enzyme was localized membrane fraction brain and PC12 cells no soluble form detected. When were differentiated into neuronal by nerve growth factor (NGF), increase...
Adipocyte-derived leucine aminopeptidase (A-LAP) is a recently identified novel member of the M1 family zinc-metallopeptidases. Transfection A-LAP cDNA into COS-7 cells resulted in secretion enzyme. In this study, recombinant was expressed Chinese hamster ovary cells, purified to homogeneity and its enzymatic properties were characterized. The enzyme active towards synthetic substrate, L-leucyl-p-nitroanilide, yielding V(max) 3.55 micromol/min/mg K(m) 1.28 mM, shown be monomeric protein with...
Although quercetin has numerous biological benefits, including preventing muscle atrophy due to disuse, no reports have been published date about the preventive effects and molecular mechanisms underlying drug-induced atrophy. Highly soluble bioavailable glycosides (QGs) were used examine inhibition of dexamethasone (DEX)-induced in vivo. Male BALB/cCrSlc mice treated with or without QGs for 7 days ad libitum, followed by addition DEX their drinking water a further days. The weight...
Dietary habits are of considerable interest as a modifiable factor for the maintenance muscle health, especially sarcopenia. The present study aimed to investigate association between dietary intake and sarcopenia prevalence in community-dwelling Japanese subjects. This cross-sectional was conducted using data from fifth survey Research on Osteoarthritis/Osteoporosis against Disability (ROAD) study, 1345 participants (437 men 908 women) aged ≥60 years were included analysis. Sarcopenia...
Arachidonic acid (ARA), docosahexaenoic (DHA), and eicosapentaenoic (EPA), which are long-chain polyunsaturated fatty acids (LCPUFAs), as well lutein (L) zeaxanthin (Z), can potentially improve brain function. However, the effect of a combination these components (LCPUFAs + LZ) on memory function in healthy older individuals remains unclear. This study aimed to determine if LCPUFAs LZ-supplemented food could Exploratory confirmatory trials (Trials 1 2, respectively) were conducted Japanese...
Scope Sesamin is a major lignan in sesame seeds and has various physiological effects. Although metabolism of sesamin by cytochrome P450 or intestinal microflora been reported, little known concerning the mass balance, pharmacokinetics, tissue distribution sesamin. Methods results Absorption, distribution, metabolism, excretion [ 14 C]sesamin were investigated after single oral dose 5 mg/kg rats. was absorbed with peak plasma radioactivity at 1.0 h declined terminal half‐life 4.7 h. The...
It remains unclear whether n-3 long-chain polyunsaturated fatty acids (LCPUFA) have a preventive effect on depression in the general population. This study investigated longitudinal association between LCPUFA intake and depressive symptoms community-dwelling Japanese participants. The participants were aged 40⁻79 years at baseline cohort study; wherein examinations; including assessment of nutritional status; biennially conducted from 1997 to 2012. subjects (n = 2335) who had Center for...
The effect of n-3 long-chain PUFA (n-3 LCPUFA) on depression in healthy subjects is unclear, and most the previous studies have focused populations eating Western diets with lower fish intake. present study investigated association between blood levels LCPUFA depressive symptoms Japanese community dwellers higher levels. A cross-sectional was conducted from 2006 to 2008, including 1050 men 1073 women aged 40 years or older National Institute for Longevity Sciences--the Longitudinal Study...
The constitutive androstane receptor CAR is a xenosensing nuclear that can be activated by natural polyphenols such as flavonoids and catechins. We examined alcoholic beverage phytochemicals for their ability to activate CAR. HepG2 cells were transfected with expression vector its reporter gene, then treated trans-resveratrol, ellagic acid, β-caryophyllene, myrcene, xanthohumol. A luciferase assay revealed acid trans-resveratrol both human mouse Since regulates many genes involved in energy...
To elucidate the effect of polyphenols contained in alcoholic beverages on metabolic stress induced by ethanol consumption, four groups mice were fed for five weeks Lieber's diet with or without ethanol, plus ellagic acid, and trans-resveratrol. Alcoholic fatty liver was observed group but not those polyphenol diets. Liver transcriptome analysis revealed that addition suppressed expression genes related to cell up-regulated alone. Conversely, involved bile acid synthesis, unsaturated...
Abstract Multifactorial lifestyle intervention is known to be more effective for ameliorating cognitive decline than single factor intervention; however, the effects of combining exercise with long-chain polyunsaturated fatty acids (LCPUFA) on elderlies' function remain unclear. We conducted a randomised, single-masked placebo-controlled trial in non-demented elderly Japanese individuals. Participants were randomly allocated LCPUFA, placebo, or no placebo (control) groups. groups performed...
Multifactorial lifestyle approaches could be more effective than a single factor for maintaining cognitive function. This study investigated the association of combining cognitively stimulating leisure activities (CSLAs), including puzzles, quizzes, and training games, with intake long-chain polyunsaturated fatty acids (LCPUFAs), docosahexaenoic acid (DHA), eicosapentaenoic (EPA), arachidonic (ARA), on function in older Japanese individuals without dementia. Participants were...