A5056577176- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- SARS-CoV-2 and COVID-19 Research
- Bacterial Identification and Susceptibility Testing
- Clostridium difficile and Clostridium perfringens research
- Antimicrobial Resistance in Staphylococcus
- Mycobacterium research and diagnosis
- Gut microbiota and health
- Probiotics and Fermented Foods
- Animal Virus Infections Studies
- interferon and immune responses
- Genomics and Phylogenetic Studies
- COVID-19 Clinical Research Studies
- Enterobacteriaceae and Cronobacter Research
- Viral gastroenteritis research and epidemiology
- Plant Pathogenic Bacteria Studies
- Microbial Metabolism and Applications
- Vibrio bacteria research studies
- Invertebrate Immune Response Mechanisms
- Pericarditis and Cardiac Tamponade
- Respiratory viral infections research
- Antimicrobial Peptides and Activities
- Long-Term Effects of COVID-19
- Bacteriophages and microbial interactions
- Drug Transport and Resistance Mechanisms
Northwestern University
2019-2024
Kelly Services (United States)
2021
Abstract Health care-associated infections such as Pseudomonas aeruginosa bacteremia pose a major clinical risk for hospitalized patients. However, these systemic are presumed to be “dead-end” P. and have no impact on transmission. Here, we use mouse infection model show that can spread from the bloodstream gallbladder, where it replicates extremely high numbers. Bacteria in gallbladder then seed intestines feces, leading transmission uninfected cage-mate mice. Our work shows is crucial of...
A critical assessment of computational hit finding experiments (CACHE) challenge was conducted to predict ligands for the SARS-CoV-2 Nsp13 helicase RNA binding site, a highly conserved COVID-19 target. Twenty-three participating teams comprised chemists and data scientists used protein structure from fragment-screening paired with advanced machine learning methods each up 100 inhibitory ligands. Across all teams, 1957 compounds were predicted subsequently procured commercial catalogs...
A critical assessment of computational hit finding experiments (CACHE) challenge was conducted to predict ligands for the SARS-CoV-2 Nsp13 helicase RNA binding site, a highly conserved COVID-19 target. Twenty-three participating teams comprised chemists and data scientists used protein structure from fragment-screening paired with advanced machine learning methods each up 100 inhibitory ligands. Across all teams, 1957 compounds were predicted subsequently procured commercial catalogs...
A critical assessment of computational hit finding experiments (CACHE) challenge was conducted to predict ligands for the SARS-CoV-2 Nsp13 helicase RNA binding site, a highly conserved COVID-19 target. Twenty-three participating teams comprised chemists and data scientists used protein structure from fragment-screening paired with advanced machine learning methods each up 100 inhibitory ligands. Across all teams, 1957 compounds were predicted subsequently procured commercial catalogs...
ABSTRACT Pseudomonas aeruginosa is a common nosocomial pathogen and major cause of morbidity mortality in hospitalized patients. Multiple reports highlight that P. gastrointestinal colonization may precede systemic infections by this pathogen. Gaining deeper insight into the dynamics carriage an essential step managing could contribute to preventing bacterial transmission progression infection. Here, we present clinically relevant mouse model relying on parenteral vancomycin pretreatment...
is a common cause of difficult-to-treat infections due to its propensity express resistance many antibiotics. For example, carbapenem-resistant
The SARS-CoV-2 nucleocapsid (N) protein is highly immunogenic, and anti-N antibodies are commonly used as markers for prior infection. While several studies have examined or predicted the antigenic regions of N, these lacked consensus structural context. Using COVID-19 patient sera to probe an overlapping peptide array, we identified six public four private epitope across some which unique this study. We further report first deposited X-ray structure stable dimerization domain at 2.05 Å...
Antimicrobial resistance (AMR) is a major challenge in the treatment of infections caused by Pseudomonas aeruginosa. Highly drug-resistant are disproportionally small subset globally distributed P. aeruginosa sequence types (STs), termed "high-risk clones." We noted that clonal complex (CC) 446 (which includes STs 298 and 446) isolates were repeatedly cultured at 1 medical center asked whether this lineage might constitute an emerging high-risk clone.We searched genomes from collections...
While several demographic and clinical correlates of coronavirus disease 2019 (COVID-19) outcome have been identified, their relationship to virological immunological parameters remains poorly defined.
SARS-CoV-2 nsp13 helicase is an essential enzyme for viral replication and a promising target antiviral drug development. This study compares the double-stranded RNA (dsRNA) unwinding activity of Omicron nsp13R392C variant, which predominant in currently circulating lineages. Using vitro gel- fluorescence-based assays, we found that both have dsRNA with equivalent kinetics. Furthermore, R392C mutation had no effect on efficiency nsp13-specific inhibitor SSYA10-001. We additionally confirmed...
is an emerging pathogen increasingly implicated in health care-associated infections. Here, we report a case of recurrent ventricular assist device-associated infection caused by multidrug-resistant
Resistance to antipseudomonal penicillins and cephalosporins is often driven by the overproduction of intrinsic β-lactamase AmpC. However, OXA-10-family β-lactamases are a rich source resistance in Pseudomonas aeruginosa. OXA have propensity for mutation that leads extended spectrum cephalosporinase carbapenemase activity. In this study, we identified isolates from subclade multidrug-resistant (MDR) high risk P. aeruginosa clonal complex CC446 with ceftazidime. A genomic analysis revealed...
Purpose: Mortality associated with Pseudomonas aeruginosa bloodstream infection (PABSI) remains high despite advances in clinical care and therapeutics. In a recent study using mice to model PABSI, the gallbladder (GB) was identified as reservoir for bacterial expansion. Furthermore, bile exposure has been linked increased antimicrobial resistance (AMR). Therefore, we asked whether patients retained gallbladders might suffer from more resistant P. (Pa) infections, extended culture...
is a common nosocomial pathogen and major cause of morbidity mortality in hospitalized patients. Multiple reports highlight that
Lacticaseibacillus (formerly Lactobacillus) rhamnosus is widely used in probiotics or food supplements to promote microbiome health and may also be part of the normal microbiota human gastrointestinal tract. However, it rarely causes invasive severe infections patients. It has been postulated that these originate from endogenous commensal reservoirs. In this report, we examine population structure investigate utility using bacterial genomics identify source infections.
ABSTRACT Larvae of Galleria mellonella (the greater wax moth) are being increasingly used as a model to study microbial pathogenesis. In this model, bacterial virulence is typically measured by determining the 50% lethal dose (LD 50 ) strain or mutant. The use G. Pseudomonas aeruginosa pathogenesis, however, challenging because extreme sensitivity larvae bacterium. For some P. strains, few 1–5 colony-forming units sufficient kill mellonella, which poses challenges for LD values. reason,...
ABSTRACT Gastrointestinal (GI) colonization by Klebsiella pneumoniae is a risk factor for subsequent infection as well transmission to other patients. Additionally, achieved many strain types that exhibit high diversity in genetic content. Thus, we aimed study strain-specific requirements K. GI applying transposon insertion sequencing three classical clinical strains: carbapenem-resistant strain, an extended-spectrum beta-lactamase producing and non-epidemic antibiotic-susceptible strain....
The Gram-negative bacterium Pseudomonas aeruginosa is often multidrug resistant, associated with global epidemic outbreaks, and responsible for significant morbidity mortality in hospitalized patients. Here, we present the draft genome sequence of BWH047, a multidrug-resistant P. clinical isolate belonging to type 235 demonstrating high levels colistin resistance.
Abstract Background Vancomycin-resistant enterococci (VRE) are nosocomial pathogens with extensive intrinsic and acquired antimicrobial resistance (AMR) mechanisms. We report a case in which intraabdominal (IA) blood cultures grew linezolid daptomycin resistant VRE (DLVRE). Methods of DLVRE bacteremia after prolonged treatment daptomycin. Results The patient was 65-year-old female history multiple abdominal surgeries who presented for elective incisional hernia repair. Her post-operative...
ABSTRACT Health care-associated infections such as Pseudomonas aeruginosa (PA) bacteremia pose a major clinical risk for hospitalized patients, and efforts to limit them are priority. The fitness pressures accounting PA virulence factors that facilitate bloodstream unclear, these presumed be “dead-end” have no impact on transmission. Here, we used mouse model show spreads from the gallbladder, where it replicates extremely high numbers. Bacteria in gallbladder then seed intestines feces,...
Abstract Background Antimicrobial resistance (AMR) poses an increasing challenge to the treatment of nosocomial pathogen Pseudomonas aeruginosa, with majority highly resistant infections caused by relatively few high-risk clones. We investigated role clonal complex 298 (CC298: ST298 and ST446) in multidrug-resistant (MDR) extensively drug-resistant (XDR) at Northwestern Memorial Hospital (NMH). Methods determined AMR 40 whole-genome sequenced CC298 isolates, including 30 from patients NMH...
Abstract Background Pseudomonas aeruginosa (PA) is a critically important healthcare-associated pathogen responsible for variety of infections including bloodstream infection (bacteremia), pneumonia, and urinary tract infection. PA bacteremia significant cause morbidity mortality, especially in immunocompromised patients; However, little known about the in-host dynamics impact individually infected patients on transmission healthcare environment. Methods We utilized animal modeling...
Abstract Background Pseudomonas aeruginosa (PA) is an important cause of healthcare-associated infections including pneumonia and bloodstream (bacteremia). PA a significant morbidity mortality, especially in immunocompromised patients those on prolonged mechanical ventilation; However, little known about the in-host infection dynamics its relationship to transmission. Methods We utilized mouse model conjunction with sequencing technology dissect pneumonia. BALB/c mice were challenged...