A5100709922- Cholesterol and Lipid Metabolism
- DNA and Nucleic Acid Chemistry
- Peroxisome Proliferator-Activated Receptors
- DNA Repair Mechanisms
- Food Quality and Safety Studies
- Asthma and respiratory diseases
- Hormonal Regulation and Hypertension
- RNA and protein synthesis mechanisms
- Electron Spin Resonance Studies
- Antioxidant Activity and Oxidative Stress
- Air Quality and Health Impacts
- Eicosanoids and Hypertension Pharmacology
- Receptor Mechanisms and Signaling
- Free Radicals and Antioxidants
- HIV/AIDS drug development and treatment
- Carbon Nanotubes in Composites
- Click Chemistry and Applications
- Nonlinear Optical Materials Research
- Biotin and Related Studies
- Nutrition, Health and Food Behavior
- Advanced biosensing and bioanalysis techniques
- HIV Research and Treatment
- Carcinogens and Genotoxicity Assessment
- RNA Research and Splicing
- Synthesis and properties of polymers
Vanderbilt University
2015-2024
Pusan National University Hospital
2023
Maria Fertility Hospital
2023
Seoul National University
1999-2021
New Generation University College
2020
Seoul National University Hospital
2020
Kangwon National University
2015-2016
Korea National Institute of Health
2014
Chungbuk National University
2009-2014
Harvard University
2014
Significant levels of the 1, N(2)-gamma-hydroxypropano-dG adducts alpha,beta-unsaturated aldehydes acrolein, crotonaldehyde, and 4-hydroxy-2E-nonenal (HNE) have been identified in human DNA, arising from both exogenous endogenous exposures. They yield interstrand DNA cross-links between guanines neighboring C.G G.C base pairs located 5'-CpG-3' sequences, as a result opening 1,N(2)-gamma-hydroxypropano-dG to form reactive that are positioned within minor groove duplex DNA. Using combination...
Recently we demonstrated that the C(7)-unsubstituted tetrahydro-1,8-naphthyridin-3-ol has more than an order of magnitude better peroxyl radical trapping activity α-tocopherol (α-TOH) in inhibited autoxidations benzene. In to prepare analogues structurally related α-TOH for further studies vitro and vivo, developed synthetic approaches C(7)-monoalkyl C(7)-dialkyl using a sequence involving (1) AgNO3-mediated hydroxymethyl addition 1,8-naphthyridine, (2) regioselective alkyllithium by cyclic...
HNE (4-hydroxynonenal), a byproduct of lipid peroxidation, reacts with nucleophilic centers on proteins. A terminal alkynyl analog (alkynyl HNE, aHNE) serves as surrogate for itself, both compounds reacting protein amine and thiol functional groups by similar chemistry. Proteins modified aHNE undergo reaction click reagent that bears azido biotin separated photocleavable linker. Peptides proteins in this way are affinity purified streptavidin beads. Photolysis the beads low intensity UV...
Despite the worldwide effect of coronavirus disease 2019 (COVID-19) pandemic, underlying mechanisms fatal viral pneumonia remain elusive. Here, we show that critical COVID-19 is associated with enhanced eosinophil-mediated inflammation when compared to non-critical cases. In addition, confirm increased T helper (Th)2-biased adaptive immune responses, accompanying overt complement activation, in group. Moreover, antibody responses and activation are pathogenesis as evidenced by formation...
Background Cimicifuga rhizome Meliae cortex Coptidis and Phellodendron have been previously shown to exhibit anti-coronavirus activity. Here, an additional 19 traditional medicinal herbal extracts were evaluated for antiviral activities in vitro. Methods A plaque assay was used evaluate the effects of extracts, concentration extract required inhibit 50% replication (EC 50 ) mouse hepatitis virus (MHV) A59 strain (MHV-A59) determined. The cytotoxic (CC each also Northern western blot analyses...
Salmonella enterica serovar Enteritidis is an important food-borne pathogen, and chickens are a primary reservoir of human infection. While most knowledge about pathogenesis based on research conducted Typhimurium, S. known to have pathobiology specific that impacts epidemiology in humans. Therefore, more information needed comparison Typhimurium. We used transposon mutagenesis identify virulence genes by assay invasiveness intestinal epithelial (Caco-2) cells chicken liver (LMH) survival...
Well-established cell culture models were combined with new analytical methods to assess the effects of small molecules on cholesterol biosynthesis pathway. The protocol, which is based sterol derivation dienolphile PTAD, was found be reliable for analysis 7-DHC and desmosterol. PTAD method applied screening a library pharmacologically active substances, effect compounds pathway determined. Of some 727 compounds, over 30 decreased in Dhcr7-deficient Neuro2a cells. examination chemical...
Reactive electrophiles generated by lipid peroxidation are thought to contribute cardiovascular disease and other oxidative stress-related pathologies covalently modifying proteins affecting critical protein functions. The difficulty of capturing analyzing the relatively small fraction modified complicates identification targets electrophiles. We recently synthesized a biotin-modified linoleoylglycerylphosphatidylcholine probe called PLPBSO ( Tallman et al. Chem. Res. Toxicol. 2007, 20,...
A small library of pharmacologically active compounds (the NIH Clinical Collection) was assayed in Neuro2a cells to determine their effect on the last step biosynthesis cholesterol, transformation 7-dehydrocholesterol (7-DHC) cholesterol promoted by reductase, DHCR7. Of some 727 Collection, over 30 significantly increased 7-DHC when at 1 μM. Active that with a Z-score +3 or greater generally gave rise modest decreases desmosterol and increases lanosterol levels. Among most identified were...
Tyrosine-derived hydroperoxides are formed in peptides and proteins exposed to enzymatic or cellular sources of superoxide oxidizing species as a result the nearly diffusion-limited reaction between tyrosyl radical superoxide. However, structure these products, which informs their reactivity biology, has not been unequivocally established. We report here complete characterization products addition superoxide, generated from xanthine oxidase, several peptide-derived radicals, horseradish...
After surgery for degenerative spinal disease by the anterior approach, degree of soft tissue swelling can be assessed simply using plain radiographs. However, there are little studies according to surgical methods or extent surgery, and no study had addressed clinical meaning determined radiography. The purpose this was evaluate significance prevertebral (PSTS) after cervical fusion with plate fixation treatment disorders.One hundred thirty-five patients that underwent augmentation...
Lipid modifications aid in regulating (and misregulating) protein function and localization. However, efficient methods to screen for a lipid's ability modify proteins are not readily available. We present strategy identify protein-reactive lipids apply it neurodevelopmental disorder, Smith-Lemli-Opitz syndrome (SLOS). Alkynyl surrogates were synthesized polyunsaturated fatty acids, phospholipids, cholesterol, 7-dehydrocholesterol (7-DHC), 7-DHC-derived oxysterol. To probe lipids, we used...
Regulating blood cholesterol (Chol) levels by pharmacotherapy has successfully improved cardiovascular health. There is growing interest in the role of Chol precursors treatment diseases. One sterol precursor, desmosterol (Des), a potential pharmacological target for inflammatory and neurodegenerative disorders. However, elevating precursor 7-dehydrocholesterol (7-DHC) inhibiting enzyme reductase linked to teratogenic outcomes. Thus, altering profile may either increase risk toward an...
The one-electron reduction of lipid hydroperoxides by low-valent iron species is believed to be a driver cellular peroxidation and associated ferroptotic cell death. We investigated reactions cholesterol 7α-OOH, the primary autoxidation product, with Fe2+ find that 7-ketocholesterol (7-KC, an oxidation product) major product under these (reducing) conditions. Mechanistic studies reveal intervention 1,2-H-atom shift upon formation 7-alkoxyl radical yield ketyl can oxidized either Fe3+ or O2...
4-Oxo-2(E)-nonenal, a lipid peroxidation-derived product, reacts with dG, dA, and dC in DNA to form heptanone (H)−etheno (ε) adducts. Among the three adducts, H−εdC is formed greatest abundance vitro reactions, it has been detected C57BL/6JAPCmin mouse model of colorectal cancer. To establish genotoxic properties this adduct, site-specifically modified oligonucleotide was synthesized incorporated into shuttle vector. The vector replicated Escherichia coli human cells. Analysis progeny...
This study was designed to evaluate the normal development of nasal septum in Koreans using sagittal MRI for valuable clinical information on septal procedures.Two hundred eighty patients who had their whole visualized midline view were selected among 3,904 with brain from January, 2004 December, 2006 at Dankook University Hospital. The a history surgery or trauma excluded. Following parameters are calculated and analyzed: lengths bony cartilage dorsum cartilage-nasal bone overlap, total...
Cannabidiol (CBD) vaping products have become widely available in the U.S. since their legalization 2018. However, little is known about respiratory health effects. Here we show that aerosolization of commercial CBD generates a reactive quinone (CBDQ) which forms adducts with protein cysteine residues. Using click chemistry and novel
Aromatic polyimides with side chain nonlinear optical chromophores have been investigated through a facile two-step synthetic route. First, various poly(hydroxy imide)s synthesized by direct thermal imidization of diaminophenol dihydrochloride salt and aromatic dianhydride monomers. The resulting bearing phenolic hydroxy groups were found to react easily the terminal group on via Mitsunobu condensation give corresponding high nonlinearities good solubility in common organic solvents....
Free radical co-oxidation of polyunsaturated lipids with tyrosine or phenolic analogues gave rise to lipid peroxide−tyrosine (phenol) adducts in both aqueous micellar and organic solutions. The novel were isolated characterized by 1D 2D NMR spectroscopy as well mass spectrometry (MS). spectral data suggest that the peroxyl radicals give stable peroxide coupling products exclusively at para position tyrosyl (phenoxy) radicals. These have characteristic 13C chemical shifts 185 ppm due...
Fluorosilicone rubber, essential in automotive and aerospace owing to its excellent chemical resistance, plays a pivotal role sealing technology, addressing the industry’s evolving demands. This study explores preparation properties of fibrillated cellulose-reinforced fluorosilicone rubber composites enhance their stiffness oil resistance. Fibrillated cellulose sourced as wet cake subjected processing modification is incorporated into matrix. The resulting are analysed by tensile compression...
Spectroscopic evidence is presented for the formation of a carbinolamine interchain cross-link in 5'-CpG-3' sequences, arising from acrolein adduct γ-OH-PdG. This may be important understanding biological processing acrolein-induced DNA damage CpG sequences.