A5101777057- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Mosquito-borne diseases and control
- SARS-CoV-2 detection and testing
- Viral Infections and Outbreaks Research
- Long-Term Effects of COVID-19
- Respiratory Support and Mechanisms
- Fire effects on ecosystems
- Respiratory viral infections research
- Biosensors and Analytical Detection
- Immune Cell Function and Interaction
- Vector-Borne Animal Diseases
- Luminescence Properties of Advanced Materials
- Viral Infections and Immunology Research
- Extracellular vesicles in disease
- Microbial Metabolism and Applications
- biodegradable polymer synthesis and properties
- Anodic Oxide Films and Nanostructures
- Viral gastroenteritis research and epidemiology
- COVID-19 and healthcare impacts
- Syphilis Diagnosis and Treatment
- Blood Coagulation and Thrombosis Mechanisms
- Animal Virus Infections Studies
Chosun University
2018-2025
Gwangju University
2025
HealthONE
2024
New Generation University College
2021
Seoul National University
2021
Gwangju Health University
2021
Kyungpook National University
2019
Despite the worldwide effect of coronavirus disease 2019 (COVID-19) pandemic, underlying mechanisms fatal viral pneumonia remain elusive. Here, we show that critical COVID-19 is associated with enhanced eosinophil-mediated inflammation when compared to non-critical cases. In addition, confirm increased T helper (Th)2-biased adaptive immune responses, accompanying overt complement activation, in group. Moreover, antibody responses and activation are pathogenesis as evidenced by formation...
Background: Remdesivir is widely used for the treatment of coronavirus disease 2019 (COVID-19), but controversies regarding its efficacy still remain.Methods: A retrospective cohort study was conducted to evaluate effect remdesivir on clinical and virologic outcomes severe COVID-19 patients from June July 2020.Primary endpoints included recovery, additional mechanical ventilator (MV) support, duration oxygen or MV support.Viral load reduction by hospital day (HD) 15 evaluated calculating...
Coronavirus disease 2019 (COVID-19) is a mild to severe respiratory illness caused by acute syndrome coronavirus 2 (SARS-CoV-2). The diagnostic accuracy of the Centers for Disease Control and Prevention (CDC)- or World Health Organization (WHO)-recommended real-time PCR (RT-qPCR) primers in clinical practice remains unproven. We conducted prospective study on RT-qPCR using an in-house-designed primer set (iNP) targeting nucleocapsid protein as well various recommended commercial primers. was...
Exosomes are potential biomarkers for disease diagnosis and treatment, as well drug carriers. However, their isolation detection remain critical issues, convenient, rapid, low-cost, effective methods necessary. In this study, we present a rapid simple method directly capturing analyzing exosomes from complex cell culture media using CaTiO3:Eu3+@Fe3O4 multifunctional nanocomposites. The nanocomposites were prepared by high-energy ball-milling used to isolate binding the hydrophilic phosphate...
Abstract We designed a highly sensitive reverse transcription nested polymerase chain reaction targeting the M-segment (NPCR-M) of severe fever with thrombocytopenia syndrome (SFTS) virus. NPCR-M was performed in parallel three other referenced PCR assays QPCR-S, PCR-M, and NPCR-S to assess their clinical usefulness as routine diagnostic techniques for SFTS. In this multi-centered prospective study, 122 blood samples from 38 laboratory-confirmed SFTS patients 85 control were used. The...
The clinical characteristics and the effect of viral RNA loads on fatality in 56 patients with severe fever thrombocytopenia syndrome (SFTS) were analyzed. non-survival group (12 patients) demonstrated a significantly higher mean age (77 years) than survival (44 patients, 65 (p = 0.003). rates 91.7% 8.3% Ct values ≥30 differed 0.001) groups, respectively. 52.4% 47.6% copy numbers ≥10,000 94.3% 5.7% <10,000 respectively 0.001). In multivariate analysis, initial Acute Psychologic Assessment...
Background/Aims: Coronavirus disease 2019 (COVID-19) is associated with acute respiratory syndrome. The mechanisms underlying the different degrees of pneumonia severity in patients COVID-19 remain elusive. This study provides evidence that eosinophil-mediated inflammation.Methods: We performed a retrospective case series three laboratory and radiologically confirmed admitted to Chosun University Hospital. Demographic clinical data on inflammatory cell lung infiltration cytokine levels were...
Scrub typhus and severe fever with thrombocytopenia syndrome (SFTS) are the 2 most common tick-borne infectious diseases in Korea. Every year, an increasing number of cases reported, which is a public health concern. Therefore, we aimed to investigate prevalence SFTS-scrub coinfection patients SFTS.Clinical samples were collected from 129 SFTS. One-step reverse-transcription polymerase chain reaction (PCR) was performed identify SFTS virus (SFTSV), real-time PCR followed by nested detect...
Abstract To investigate the specific genomic features and mutation pattern, whole near-complete SARS-CoV-2 genome sequences were analyzed. Clinical samples collected from 18 COVID-19–positive patients subjected to nucleic acid purification. Cell culture was performed extract various isolates. Whole-genome analysis using next-generation sequencing, phylogenetic analyses conducted determine genetic diversity of The sequencing data identified 8 protein-coding regions with 17 mutated proteins....
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants continue to emerge during the ongoing disease 2019 (COVID-19) pandemic. Contrasting studies on omicron variant have demonstrated higher viral loads in different clinical specimens, which is consistent with its high transmissibility. We investigated load specimens that were infected SARS-CoV-2 wild-type, delta, and variants, we analyzed diagnostic accuracy of upper lower for these variants. performed nested reverse...
Introduction Coronavirus disease 2019 (COVID-19) is caused by SARS-CoV-2. We investigated the antibody response against SARS-CoV-2 until 1 year after symptom onset. Methods collected 314 serum samples from 97 patients with COVID-19. Antibody responses were tested using an indirect immunofluorescence assay (IFA), enzyme-linked immunosorbent (ELISA), and plaque reduction neutralization test (PRNT) to detect specific neutralizing antibodies. Results The positivity rates for antibodies at a 1:10...
Abstract Scrub typhus is a major acute febrile disease in the Asia–Pacific region. The purpose of present study to investigate clinical usefulness real-time PCR (Q-PCR) 16S rRNA for diagnosis scrub typhus. We examined blood specimens from 148 adult patients who were confirmed have September 2008 December 2009. Among patients, 36 treated with antibiotics before admission. To evaluate Q-PCR, we compared its diagnostic accuracy following methods: nested (N-PCR) targeting gene encoding 56-kDa...
Rickettsial diseases associated with the spotted fever group constitute a growing number of newly identified Rickettsia pathogens and their tick vectors in various parts world. At least 15 distinct species belonging to six genera have shown presence raoultii. Herein, we report detection R. raoultii ticks from Republic Korea (ROK). Thirty-five were collected 29 patients bites Gwangju Metropolitan City, Jeollanam Province, ROK. The using molecular, morphological, taxonomic characteristics. All...
Predictive studies of acute respiratory distress syndrome (ARDS) in patients with coronavirus disease 2019 (COVID-19) are limited. In this study, the predictors ARDS were investigated and a score that can predict progression to COVID-19 pneumonia was developed. All who diagnosed between February 1, 2020, May 15, at five university hospitals Korea enrolled. Their demographic, clinical, epidemiological characteristics outcomes collected using World Health Organization Case Report Form. A...
Abstract This study analyzed HGA and SFTS in patients with suspected tick-borne infection by focusing on key differences that clinicians can easily recognize. A retrospective analysis was performed confirmed or 21 Korean hospitals from 2013 to 2020. scoring system developed multivariate regression accuracy assessment of clinically discriminable parameters performed. The logistic revealed sex (especially male sex) (odds ratio [OR] 11.45, P = 0.012), neutropenia (< 1500) (OR 41.64, <...
Here, we aimed to investigate the diagnostic value of a serological assay using nucleocapsid protein developed for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection and evaluated its performance three commercial enzyme-linked immunosorbent assays (ELISAs), namely, Standard E 2019 novel disease (COVID-19) total antibody (Ab) ELISA (SD Biosensor), EDI COVID-19 IgG IgM ELISA. A recombinant (rNP) was expressed from plants Escherichia coli serum Ab. We prospectively collected...
Background: Rapid identification and effective isolation are crucial for curbing the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). To meet this requirement, antigen-detection rapid diagnostic tests (Ag-RDTs) essential. Methods: Between February 2020 August we performed a cohort study patients with confirmed COVID-19. The clinical performance Ag fluorescence immunoassay (FIA) Gold was evaluated compared in parallel genomic subgenomic real-time reverse...
ABSTRACT. Hemorrhagic fever with renal syndrome (HFRS) is confirmed by the isolation of hantavirus from serum, detection virus-specific IgM, or a four-fold change in IgG titers during acute and convalescent periods measured using an immunofluorescence assay (IFA). However, these tests are inefficient for early diagnosis. Therefore, this study investigated usefulness reverse-transcriptase nested polymerase chain reaction (RT-nPCR) diagnosis HFRS clinical samples such as urine serum....
To determine the relationship between viral kinetics and severity of disease in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, we investigated compared loads patients with 2019 (COVID-19; caused by SARS-CoV-2), stratified symptoms severity. We determined 100 diagnosed COVID-19 at Chosun University Hospital February 2020 May 2021 analyzed differences asymptomatic, symptomatic, nonsurvivor who died those survived. Clinical samples, comprising specimens (sputum samples...
The incidence of mite- and tick-borne infectious disease is increasing with climate change the development diagnostic tools. Tick-borne diseases include Lyme disease, anaplasmosis, ehrlichiosis, severe fever thrombocytopenia syndrome (SFTS), Japanese spotted fever. Rickettsial pox scrub typhus are mite-borne diseases. Scrub SFTS most common in Korea, respectively. They often difficult to diagnose at an early stage disease. To make a definite diagnosis polymerase chain reaction (PCR) tests or...
Background: Owing to the coronavirus disease 2019 (COVID-19) pandemic, there is a shortage of hospital wards accommodate increasing number patients, especially in intensive care units. Healthcare systems are collapsing many countries. Therefore, it necessary reduce isolation time. Methods: We examined effect lopinavir/ritonavir administration patients with SARS-CoV-2. To assess viral load, duration and clearance viable virus; cell culture RT-PCR were performed parallel. Results: No...