Abstract The dense structure and the high‐density negative charge of cartilage matrix impede drug penetration into cartilage. Effectively breaking through these barriers transporting target drugs to is currently difficult. Herein, an adhesive hydrogel microsphere characterized by its positively charged nanosized secondary that allows it penetrate deep guidance introduced as efficient delivery system for adhesion, penetration, chondrocytes targeting solves problem drug‐penetration impedance...

Abstract Loading hydrogels with bioactive agents is an important method for expanding the functional application of hydrogels. However, how to improve local administration and slow release drugs from a hydrogel challenge when using loaded drugs. In this paper, we first developed adhesive liposomes (A-LIP) BMP-2. Then, incorporated A-LIP into PEG based on coordinated cross-linking principle SH-PEG Ag + , fabricating injectable, antibacterial self-healing multifunctional drug delivery system....

Mechanical activation of fibroblasts, caused by friction and transforming growth factor-β1 recognition, is one the main causes tissue adhesions. In this study, we developed a lubricated gene-hydrogel patch, which provides both motion lubrication microenvironment gene therapy. The patch's outer layer composed polyethylene glycol polyester hydrogel. hydrogel forms hydrogen bonds with water molecules to create layer, it also serves as delivery library for long-term silencing. Under...

Soft tissue remodeling is a sophisticated process that sequentially provides dynamic biological signals to guide cell behavior. However, capturing these within hydrogel and directing over time has still been unrealized owing the poor comprehension of physiological processes. Here, bio-mimicking designed via thiol-ene click reaction capture early physical signal triggered by inflammation, chemical provided with chemokine natural adhesion sites, which guaranteed precise soft remodeling. This...

Lesion positioning therapy optimizes medical treatment by directly targeting lesions. However, strong physical barriers greatly hinder its wide use. Here, the Chinese acupuncture needles (CA-needles) with a screw-thread structure at tip (ST-needle) and hydrogel function of adhesive metal loaded drug sustained-release are designed, through minimally invasive precise lesions ST-needles, dry-wet conversion absorbing fluids swelling, rotation back is precisely positioned in subchondral bone...

Abstract Scarring rather than regeneration, is an inevitable outcome of unbalanced amplifications inflammation‐destructive signals and atresia the regenerative niche. However, identifying effectively hedging against risk scarring realizing conversion cues remain difficult. In this work, a immune strategy based microfibrous membrane (Him‐MFM), by tethering distearoyl phosphoethanolamine layer‐supported copoly(lactic/glycolic acid) electrospun fibers with identified CD11b + /CD68 subpopulation...

The damaged endometrium and the formation of fibrosis are key barriers to pregnancy further lead infertility. However, how promote repair is always a challenge. Here, bioactive injectable self-healing hydrogel developed by physically combination thiolated polyethylene (PEG), Cu2+ cell-free fat extract (CEFFE, CF) for endometrial regeneration fertility. By inheriting advantages various active proteins contained in CEFFE, it could induce overall microenvironment intrauterine adhesion (IUA). In...

Inspired by the superlubricated surface (SLS) of ice, which consists an ultrathin and contiguous layer surface-bound water, we built a SLS on polycaprolactone (PCL)/poly(2-methacryloxyethylphosphorylcholine) (PMPC) composite nanofibrous membrane via electrospinning under controlled relative humidity (RH). The zwitterionic PMPC nanofiber provided bound thus generating hydration lubrication surface. Prepared 20% RH, electrospun PCL/PMPC nanofibers reached minimum coefficient friction (COF)...

Abstract The localization of lesion cells and targeted regulation organelle function can promote the repair. However, conventional is difficult to reach deep lesions target mitochondria. In this study, dynamic spiral Mosaic technology ued construct heat transfer microneedles with micro/nano hydrogel microspheres (ST‐needle), using molecular chain motion in response stimulation regulate adhesion non‐oriented triblock polymer, as a dual delivery system for energy biological factors. ST‐needle...

Purpose: Stroke-associated pneumonia (SAP), a common complication in acute ischemic stroke (AIS) patients, is associated with poor prognosis after AIS. Inflammation plays an important role the development of SAP. In this study, we aimed to explore association between monocyte-to-lymphocyte ratio (MLR) and SAP AIS patients. Methods: We continuously enrolled 972 was diagnosed by two trained neurologists confirmed radiography, meeting modified Centers for Disease Control Prevention criteria....

Abstract Geometry and angles play crucial roles in cellular processes; however, its mechanisms of regulation remain unclear. In this study, a series three dimensional (3D)‐printed microfibers with different geometries is constructed using near‐field electrostatic printing technique to investigate the regulatory geometry on stem cell function bone regeneration. The scaffolds precisely mimicked dimensions high porosity interoperability. Compared other spatial topography angles, 90° topology...

Rationale: Peri-prosthetic osteolysis (PPO) is mainly induced by wear particles and represents the leading cause of implant failure revision surgery. Previous studies have identified mitigation particle-induced inflammation bone resorption as main approaches to treat PPO. Recently, reduction formation around prosthesis was a major factor in development Acetyl-11-keto-β-boswellic acid (AKBA), derivative frankincense, has been shown play potential role metabolism. However, whether AKBA...

Abstract Background Ctns −/− mice, a mouse model of infantile nephropathic cystinosis, exhibit hypermetabolism with adipose tissue browning and profound muscle wasting. mice are 25(OH)D 3 1,25(OH) 2 D insufficient. We investigated whether vitamin repletion could ameliorate wasting in mice. Methods Twelve‐month‐old wild‐type controls were treated (75 μg/kg/day 60 ng/kg/day, respectively) or an ethylene glycol vehicle for 6 weeks. Serum chemistry parameters energy homeostasis measured....

Abstract Clustered short palindrome repeats with regular intervals, abbreviated as CRISPR, and functions a self‐defense system for prokaryotes, detecting particular pathogenic nucleic acid, interfering the of exoteric DNA, protecting them against foreign invaders. In recent years, CRISPR has attracted increasing interests in vitro diagnostic field because its inherent allele specificity, which is one critical factors successful application this technology development high‐precision treatment...

Cytokines such as IL-6, TNF-α and IL-1β trigger inflammatory cascades which may play a role in the pathogenesis of chronic kidney disease (CKD)-associated cachexia. CKD was induced by 5/6 nephrectomy mice. We studied energy homeostasis Il1β-/-/CKD, Il6-/-/CKD Tnfα-/-/CKD mice compared with wild type (WT)/CKD controls. Parameters cachexia phenotype were completely normalized Il1β-/-/CKD but only partially rescued tested effects anakinra, an IL-1 receptor antagonist, on CKD-associated WT/CKD...

Advances in oncologic treatment modalities and wide resection have made limb salvage procedures calcaneal malignancy increasingly possible. However, reconstructions of the remain a major surgical challenge because rarity specific anatomy this condition.we retrospectively reviewed five patients who had primary underwent total calcanectomy with distally pedicled osteocutaneous folded fibular flap between 2001 2007. The diagnoses included chondrosarcoma 2, Ewing's sarcoma 2 osteosarcoma 1....

Abstract Electrospun fiber membranes have been extensively researched for tissue repair; however, angiogenesis has a difficult problem because the biomaterials used to prepare cannot promote by themselves. To endow with function of promoting and oral mucosa regeneration, surface-aminated liposomes (NH 2 -LIPs) encapsulating leptin, as well silk (SF) that immersed overnight in polydopamine (PDA) solution were synthesized. The NH -LIPs then grafted onto surfaces SF through reactions between...