A5001506160- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- Influenza Virus Research Studies
- interferon and immune responses
- Immune Cell Function and Interaction
- Mosquito-borne diseases and control
- vaccines and immunoinformatics approaches
- Viral gastroenteritis research and epidemiology
- Long-Term Effects of COVID-19
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Zoonotic diseases and public health
- Diabetes and associated disorders
- Inflammasome and immune disorders
- Microbial infections and disease research
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- COVID-19 epidemiological studies
- Data-Driven Disease Surveillance
- Oral microbiology and periodontitis research
- Viral Infections and Outbreaks Research
- Immune cells in cancer
- Gut microbiota and health
Sun Yat-sen University
2021-2025
Wuhan Institute of Virology
2012-2013
Chinese Academy of Sciences
2012-2013
A recent mutation analysis suggested that Non-Structural Protein 6 (NSP6) of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a key determinant viral pathogenicity. Here, by transcriptome analysis, we demonstrated inflammasome-related NOD-like receptor signaling was activated in SARS-CoV-2-infected lung epithelial cells and Disease 2019 (COVID-19) patients' tissues. The induction inflammasomes/pyroptosis patients with severe COVID-19 confirmed serological markers....
Abstract Bats are reservoir hosts for many zoonotic viruses. Despite this, relatively little is known about the diversity and abundance of viruses within individual bats, hence frequency virus co-infection spillover among them. We characterize mammal-associated in 149 bats sampled from Yunnan province, China, using an unbiased meta-transcriptomics approach. This reveals a high (simultaneous infection bat individuals by multiple viral species) animals studied, which may turn facilitate...
Increasing evidence suggests that dysregulated immune responses are associated with the clinical outcome of coronavirus disease 2019 (COVID-19). Nucleocapsid protein (NP)-, spike (S)-, receptor binding domain (RBD)- specific immunoglobulin (Ig) isotypes, IgG subclasses and neutralizing antibody (NAb) were analyzed in 123 serum from 63 hospitalized patients severe, moderate, mild or asymptomatic COVID-19. Mild to modest correlations found between severity antigen serum, which IgG1 IgG3...
Abstract B cell response plays a critical role against SARS-CoV-2 infection. However, little is known about the diversity and frequency of paired antigen-specific BCR repertoire after Here, we performed single-cell RNA sequencing VDJ using memory plasma cells isolated from five convalescent COVID-19 patients, analyzed spectrum transcriptional heterogeneity antibody immune responses. Via linking to antigen specificity through (LIBRA-seq), identified distinct activated subgroup ( CD11c high...
The development of a universal influenza vaccine to elicit broad immune responses is essential in reducing disease burden and pandemic impact. In this study, the mosaic design strategy genetic algorithms were utilized optimize seasonal A virus (H1N1, H3N2) hemagglutinin (HA) neuraminidase (NA) antigens, which also contain most potential T-cell epitopes. These immunogens then expressed as virus-like particles (VLPs) using baculovirus expression system. immunogenicity protection effectiveness...
Abstract Human bocavirus (HBoV) mainly infects young children. Although many infected children suffer from respiratory or gastroenteric tract diseases, an association between HBoV and these diseases is not definite. Because modulation of type I IFN crucial for viruses to establish efficient replication, in this study, we tested whether modulates production. We observed that a nearly full-length clone significantly reduced both Sendai virus (SeV)- poly(deoxyadenylic-thymidylic) acid-induced...
Following the lifting of COVID-19 non-pharmaceutical interventions in China, respiratory infections surged, though specific causes remained unclear. This study provided a comprehensive overview infectome patients with acute febrile illness (AFRI) to inform disease surveillance. Between March 2023 and February 2024, 1,163 oropharyngeal swabs from AFRI 338 healthy individuals were collected Shenzhen. Meta-transcriptomic sequencing was employed for microbial analysis. We identified 14 viruses...
Despite many studies on the immune characteristics of Coronavirus disease 2019 (COVID-19) patients in progression stage, a detailed understanding pertinent cells recovered is lacking. We performed single-cell RNA sequencing samples from COVID-19 and healthy controls. created comprehensive landscape with more than 260,000 peripheral blood mononuclear (PBMCs) 41 by integrating our dataset previously reported datasets, which included collected between 27 47 days after symptom onset. According...
ABSTRACT Companion animals such as cats and dogs harbor diverse microbial communities that can potentially impact human health due to close frequent contact. To better characterize their total infectomes assess zoonotic risks, we characterized the overall of companion (cats dogs) evaluated potential risks. Meta-transcriptomic analyses were performed on 239 samples from collected across China, identifying 24 viral species, 270 bacterial genera, two fungal genera. Differences in microbiome...
Precise regulation of innate immunity is crucial for maintaining optimal immune responses against infections. Whereas positive IFN signaling elicits rapid type I IFNs, negative equally important in preventing the production superfluous IFNs that can be hazardous to host. The regulators pathway are known main targets viruses antagonize system. Whether target remains fully investigated. In this study, we report structural protein VP2 human Bocavirus modulates by targeting ring finger 125...
c-FLIP functions as a dual regulator of apoptosis and inflammation, yet its implications in Zika virus (ZIKV) infection remain partially understood, especially the context ZIKV-induced congenital syndrome (CZS) where both inflammation play pivotal roles. Our findings demonstrate that promotes ZIKV placental cells myeloid-derived macrophages, involving caspase-8/3-mediated apoptosis. Moreover, our observations reveal augments multiple tissues, including blood cell, spleen, uterus, testis,...
Orthobunyaviruses are a group of viruses with significant public and veterinary health importance. These mainly transmitted through mosquito-, midge-, tick-vectors, endemic to various regions the world. Ebinur Lake virus (EBIV), newly identified member Orthobunyavirus , was isolated from Culex mosquitoes in Northwest China. In present study, we aimed characterize pathogenesis host immune responses EBIV BALB/c mice, as an animal model. Herein, determined that mice highly susceptible...
Pre-existing cross-reactive immunity among different coronaviruses, also termed immune imprinting, may have a comprehensive impact on subsequent SARS-CoV-2 infection and COVID-19 vaccination effectiveness. Here, we aim to explore the interplay between pre-existing seasonal coronaviruses (sCoVs) antibodies humoral induced by vaccination.We first collected serum samples from healthy donors prior pandemic individuals who had received post-pandemic in China, levels of IgG against sCoVs were...
Human bocavirus (HBoV), closely related to canine minute virus (MVC) and bovine parvovirus (BPV), is a new member of the Bocavirus genus within Parvoviridae family. The non-structural protein NP1 HBoV nuclear localized plays an important role in DNA replication as well evasion host innate immunity. In current study, we provide first evidence that possesses non-classical localization signal (ncNLS) (amino acids 7-50). Embedded this ncNLS classical bipartite (cNLS) 14-28), capable transporting...
Zinc ion as an enzyme cofactor exhibits antiviral and anti-inflammatory activity during infection, but circulating zinc level Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection is unclear. This study aimed to evaluate serum in Disease 2019 (COVID-19) patients healthy subjects, well its correlation with antibodies against SARS-CoV-2. 114 COVID-19 48 subjects (38 volunteers 10 close contacts of COVID-19) were included. concentration levels SARS-CoV-2 Spike 1 + proteins,...
BackgroundInfluenza viruses pose a persistent threat to global public health, necessitating the development of innovative and broadly effective vaccines.MethodsThis study focuses on multiepitope vaccine (MEV) designed provide broad-spectrum protection against different influenza viruses. The MEV, containing 19 B-cell linear epitopes, 7 CD4+ T cells, 11 CD8+ cells epitopes identified through enzyme-linked immunospot assay (ELISPOT) in infected mice, was administered regimen two doses DNA...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is ongoing and multiple studies have elucidated its pathogenesis, however, the related- microbiome imbalance caused by SARS-CoV-2 still not clear. In this study, we comprehensively compared composition associated function alterations in oropharyngeal swabs of healthy controls disease 2019 (COVID-19) patients with moderate or symptoms metatranscriptomic sequencing. We did observe a reduced alpha-diversity but...
A single-nucleotide polymorphism (SNP) rs12252-C of interferon-induced transmembrane protein 3 (IFITM3), resulting in a truncated IFITM3 lacking 21 N-terminus amino acids, is associated with severe influenza infection the Chinese population. However, effect on vaccination and underlying mechanism poorly understood. Here, we constructed mouse model deletion acids at (NΔ21) then compared antibody response between Quadrivalent vaccine (QIV) immunized wild-type (WT) mice NΔ21 mice. Significantly...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection causes asymptomatic or mild symptoms, even rare hospitalization in children. A major concern is whether the pre-existing antibodies induced by low pathogenic human coronaviruses (LPH-CoVs) children can cross-react with SARS-CoV-2. To address this unresolved question, we analyzed spike (S)-specific immunoglobin (Ig) G against LPH-CoVs and cross-reactive SARS-CoV-2 658 serum samples collected from prior to outbreak. We...
Vaccination is an effective way to protect from influenza virus infection. Among the new candidates of vaccines, virus-like particles (VLPs) seem be promising. Here, we generated 2 types H5N1 VLPs by co-expressing Env (envelope protein) and murine leukemia (MLV) Gag–Pol. co-transfection pHCMV-wtH5 or pHCMV-mtH5 with pSV-Mo-MLVgagpol pHCMV-N1 were named as wtH5N1 mtH5N1 VLPs. The plasmid encoded wild-type hemagglutinin (HA) (wtH5) A/swine/Anhui/ca/2004 (H5N1) a multibasic cleavage site, while...
Bats are reservoir hosts for many zoonotic viruses. Despite this, relatively little is known about the diversity and abundance of viruses within bats at level individual animals, hence frequency virus co-infection inter-species transmission. Using an unbiased meta-transcriptomics approach we characterised mammalian associated present in 149 sampled from Yunnan province, China. This revealed a high species spillover among animals studied, with 12 shared different bat species, which turn...
Abstract Bats are reservoir hosts for many zoonotic viruses. Despite this, relatively little is known about the diversity and abundance of viruses within bats at level individual animals, hence frequency virus co-infection inter-species transmission. Using an unbiased meta-transcriptomics approach we characterised mammalian associated present in 149 sampled from Yunnan province, China. This revealed a high species spillover among animals studied, with 12 shared different bat species, which...