A5067141570- Virus-based gene therapy research
- Magnetic confinement fusion research
- Pancreatic function and diabetes
- Skin and Cellular Biology Research
- Glioma Diagnosis and Treatment
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Neurogenesis and neuroplasticity mechanisms
- Immunotherapy and Immune Responses
- Particle accelerators and beam dynamics
- Metabolism, Diabetes, and Cancer
- Fusion materials and technologies
- Diabetes and associated disorders
- Laser-Plasma Interactions and Diagnostics
- SARS-CoV-2 and COVID-19 Research
- Animal Genetics and Reproduction
- RNA Research and Splicing
- Diabetes Management and Research
- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- Nanoplatforms for cancer theranostics
- Cancer therapeutics and mechanisms
- Cellular Mechanics and Interactions
- Seaweed-derived Bioactive Compounds
- Endoplasmic Reticulum Stress and Disease
Guangdong Ocean University
2025
Soochow University
2019-2024
Changshu No.1 People's Hospital
2024
Saluda Medical (Australia)
2021
Vanda Pharmaceuticals (United States)
2021
University of Chinese Academy of Sciences
2021
Menlo School
2021
Institute of Plasma Physics
2013-2019
Chinese Academy of Sciences
1992-2019
First Affiliated Hospital of Soochow University
2019
Mutations in isocitrate dehydrogenase 1 (IDH1) or 2 (IDH2) are found a subset of gliomas. Among the many phenotypic differences between mutant and wild-type IDH1/2 gliomas, most salient is that glioma patients demonstrate markedly improved survival compared with patients. To address mechanism underlying superior clinical outcome patients, we investigated whether overexpression IDH1R132H protein could affect response to therapy context an isogenic cell background. Stable clonal U87MG U373MG...
Somatic mutation of isocitrate dehydrogenase 1 (IDH1) is now recognized as the most common initiating event for secondary glioblastoma, a brain tumor type arising with high frequency in frontal lobe. A puzzling feature IDH1 selective manifestation glioma only neoplasm frequently associated early postzygotic occurrence this genomic alteration. We report here that IDH1(R132H) exhibits growth-inhibitory effect abrogated presence glutamate 2 (GLUD2), hominoid-specific enzyme purportedly...
Glucose catabolism induces the expression of L-type pyruvate kinase (L-PK) gene through glucose response element (GIRE). The metabolic pathway used by after its phosphorylation to 6-phosphate glucokinase induce L-PK in hepatocytes remains unknown. sugar alcohol xylitol is metabolized xylulose 5-phosphate, an intermediate nonoxidative branch pentose phosphate pathway. In this study, we demonstrated that at low concentration (O.5 mM) induced L-PK/CAT construct glucose-responsive mhAT3F...
Amplification or overexpression of growth factor receptors is a frequent occurrence in malignant gliomas. Using both expression profiling and situ hybridization, we identified insulin-like 2 (IGF2) as marker for subset glioblastomas (GBMs) that lack amplification EGF receptor. Among 165 primary high-grade astrocytomas, 13% grade IV tumors 2% III expressed IGF2 mRNA levels >50-fold the sample population median. IGF2-overexpressing frequently displayed PTEN loss, were highly proliferative,...
Introduction Carrageenan oligosaccharides are of significant interest due to their diverse bioactivities, necessitating efficient methods for production. To this day, the discovery and isolation microorganisms capable effectively degrading carrageenan is still crucial production oligosaccharides. In addition, there no current reports bacteria genus Photobacterium secreting κ-carrageenanase or carrageenan. Methods study, strain GDSX-4 was obtained from Gracilaria coronopifolia after...
Paradigms of insulin gene therapy for type 1 diabetes should incorporate vigorous control expression to be effective in correcting postprandial hyperglycemia and safe preventing fasting hypoglycemia. We hypothesize that hepatic auto-regulated positively by glucose negatively might both the treatment diabetes. Expression 6-phosphatase (G6Pase) liver is stimulated suppressed insulin. The G6Pase promoter incorporated with intronic enhancers aldolase B was used direct streptozotocin-induced...
Damage in COVID-19 results from both the SARS-CoV-2 virus and its triggered overactive host immune responses. Therapeutic agents that focus solely on reducing viral load or hyperinflammation fail to provide satisfying outcomes all cases. Although cellular factors have been extensively profiled identify potential anti-COVID-19 targets, new drugs with significant efficacy remain be developed. Here, we report potent preclinical of ALD-R491, a vimentin-targeting small molecule compound, treating...
Vimentin is an intermediate filament protein with diverse roles in health and disease far beyond its structural functions. Exosomes or small extracellular vesicles (sEVs) are key mediators for intercellular communication, contributing to tissue homeostasis the progression of various diseases, especially metastasis cancers. In this study, we evaluated a novel vimentin-binding compound (R491) anti-cancer activities cancer exosome release. The R491 induced rapid reversible intracellular...
Transgenic mice expressing either human proinsulin cDNA or mutated in the liver were created. The was to generate a protein cleavable by ubiquitous prohormone convertase furin, thus leading mature insulin peptide. All transgenic lines expressed C‐peptide blood, whose level varied according nutritional conditions. High performance liquid chromatography fractionation of mouse serum revealed that mutant effectively processed into vivo. This model provides useful tool for further prospects gene...
A dual gas puff imaging (GPI) system has been successfully assembled on the Experimental Advanced Superconducting Tokamak (EAST) and applied for study of dithering L–H transition in 2012 spring campaign. new method i.e., dynamic programming based time-delay estimation technique, to 64 × pixels GPI video images yield time-dependent two-dimensional velocity fields at plasma edge EAST. Local poloidal flow velocities up ∼−3 km s−1 (in electron diamagnetic direction) radial ∼−2 (inward) are found...
A novel bi-directional Retarding Field Analyzer (RFA) probe has been installed on a fast reciprocating drive system the Experimental Advanced Superconducting Tokamak (EAST) to measure ion temperature and electron fluxes. Langmuir assembly was added top of RFA head control position relative last closed flux surface have possibility density as well. Except temperature, fluxes from both drift sides measured during lower hybrid current drive. The also used electrons associated with edge...
Metastasizing cells express the intermediate filament protein vimentin, which is used to diagnose invasive tumors in clinic. However, role of vimentin cell motility, and if assembly non-filamentous variants into filaments regulates migration remains unclear. We observed that vimentin-targeting drug ALD-R491 increased stability filaments, by reducing and/or disassembly. ALD-R491-treatment also resulted more bundled disorganized an pool vimentin. This was accompanied a reduction size...
Vimentin, an intermediate filament protein, crucially contributes to the pathogenesis of inflammatory bowel disease (IBD) by interacting with genetic risk factors, facilitating pathogen infection, and modulating both innate adaptive immune responses. This study aimed demonstrate preclinical proof-of-concept for targeting vimentin therapeutically in IBD across diverse etiologies.The small molecule compound ALD-R491 was assessed binding using microscale thermophoresis, off-target effects via...
One challenge in long-pulse and high performance tokamak operation is to control the edge localized modes (ELMs) reduce transient heat load on plasma facing components. Minute-scale discharges H-mode have been achieved repeatedly Experimental Advanced Superconducting Tokamak (EAST) since 2016 campaign understanding characteristics of ELMs these can be helpful for effective ELM discharges. The kinetic profile diagnostics recently developed EAST make it possible perform pedestal stability...
These researchers proposed the heterogeneity of hypothalamic neural progenitor cells. Other than traditional radial glial (hRG) cells, which are predominantly located in ventricular zone, there additional basal progenitors mantle named as zone (hmRG) The utilized lineage tracing to reveal movement and division properties hRG hmRG Meanwhile, they applied single cell RNA sequencing investigate diversity progenitors, found that HMGA2, a newly discovered marker, is involved E2F1 pathway,...
An MMLV‐based retroviral vector containing the chloramphenicol acetyl transferase reporter gene under control of a glucose‐dependent internal promoter derived from L‐type pyruvate kinase was constructed. After transfection into psi‐CRIP packaging cells, clones producing recombinant retrovirus were selected. These retroviruses used to infect cultured established hepatocytic cells whose endogenous is transcriptionally regulated by glucose. In infected as responsive glucose gene, and activation...
D. Don is a widely cultivated Chinese herbal medicine known for its medicinal properties. However, differences in the spatial distribution of metabolites, accumulation patterns flavonoids, and pharmacological activities between aerial parts roots