Rhabdomyolysis-induced acute kidney injury (AKI) accounts for about 10 to 40% of all cases AKI. It is known that N-acetylcysteine (NAC) effective in various experimental renal models; however, little information available the rat model glycerol-induced rhabdomyolysis. In this study, we hypothesize NAC plays a renoprotective role via anti-apoptotic pathway.Male Sprague-Dawley rats were divided into four groups: (i) saline control group, (ii) NAC-treated group (N-acetylcysteine) (150 mg/kg),...

Cisplatin has multiple cellular targets and modes of action that lead to nephrotoxicity. This suggests novel therapies act at cisplatin target sites may be effective. We tested whether human adipose tissue-derived mesenchymal stem cells (Ad-MSCs) can affect protect against cisplatin-induced kidney damage. Rats were divided into four groups: control, infused with Ad-MSCs, injected cisplatin, followed by infusion Ad-MSCs. Animal survival renal function decreased histological damage was...

Abstract Background Since the recent establishment of a murine model eosinophilic chronic rhinosinusitis with nasal polyps ( CRS w NP ), both development new drugs for treatment or prevention and elucidation their pathogenesis have been feasible. We investigated therapeutic effects resveratrol on its mechanism action using model. Methods After induction , were tested compared those triamcinolone acetonide. Histopathologic changes evaluated hematoxylin eosin overall inflammation, Sirius red...

Cisplatin is a chemotherapeutic drug but induces acute kidney injury (AKI). Cisplatin-induced AKI depends on several signaling pathways leading to apoptosis in tubular epithelial cells. Glutamine substrate for the synthesis of glutathione, most abundant intracellular thiol and antioxidant, plays an important role protecting cells from induced by different stimuli. In present study, we investigated protective effect glutamine cisplatin-induced AKI. Rats were divided into control, glutamine,...

This study examined the functional significance of heme oxygenase-1 (HO-1) expression on renal injury induced by ureteral obstruction in rat kidney. Male Sprague-Dawley rats were divided into three groups, after which unilateral (UUO) was performed: untreated (group 1), treated with 30 mg/kg body wt hemin 2), and 50 microg/kg zinc (alpha) protoporphyrin eta (ZnPP) 3). After 7 14 d, histologic changes HO-1, Bcl-2, Bad, TGF-beta, cleaved caspase-3 examined. Tubular lumens dilated epithelial...

Tubular cell apoptosis is linked to the development of acute kidney injury (AKI), which a frequent complication traumatic rhabdomyolysis. The 14-3-3 protein, multifunctional regulatory binds variety apoptotic proteins and target c-Jun N-terminal kinase (JNK) in death signaling pathway. Therefore, we examined whether JNK phosphorylates downstream mitochondrial pathway mediates myoglobinuric determine these events are regulated by glutamine, known induce heat shock protein 70 (Hsp70), or...

Radiation therapy is a highly effective treatment for patients with solid tumors. However, it can cause damage and inflammation in normal tissues. Here, we investigated the effects of alpha-lipoic acid (ALA) as radioprotection agent small intestine mouse model.Whole abdomen was evenly irradiated total dose 15 Gy. Mice were treated either ALA (100 mg/kg, intraperitoneal injection [i.p.]) or saline (equal volume, i.p.) prior to radiation 100 mg/kg/day 3 days. Body weight, food intake,...

// Jin Hyun Kim 1, 2 , Kyung Mi 3 Myeong Hee Jung 1 Hwa 2, 4 Ki Mun Kang 5 Bae Kwon Jeong Pyeong Je Park Seung Hoon Woo Biomedical Research Institute, Gyeongsang National University Hospital, Jinju, Gyeongnam, Republic of Korea Institute Health Science, Department Otolaryngology, Internal Medicine, Radiation Oncology, School Medicine and Correspondence to: Woo, email: lesaby@hanmail.net Keywords: alpha lipoic acid, salivary gland, radiation, Nox-2, complication Received: December 27, 2015...

Acute kidney injury (AKI) is the most common condition in hospitalized patients. As ischemia/reperfusion-induced AKI (IR-AKI) as a major contributor to end-stage disease, an effective therapeutic intervention for IR-AKI imperative. Erythropoietin (EPO) potent stimulator of erythroid progenitor cells and significantly upregulated during hypoxia. Here, we investigated renoprotective effects EPO mouse model. Mice were assigned sham, only, IR only groups, group was treated with prior injury....

Contrast-induced acute kidney injury (CI-AKI) is the third most common cause of hospital-acquired renal failure, with an incidence 11%. However, disease mechanism remains unclear, and no effective treatment available. Paricalcitol has been reported to be in animal models injury. We hypothesized that paricalcitol could play a renoprotective role against CI-AKI. Rats were divided into control, paricalcitol, contrast, paricalcitol-plus-contrast groups. used previously published protocol produce...

Exposure of the thyroid to radiation during radiotherapy head and neck is often unavoidable. The present study aimed investigate protective effect α-lipoic acid (ALA) on radiation-induced injury in rats. Rats were randomly assigned four groups: healthy controls (CTL), irradiated (RT), received ALA before irradiation (ALA + RT), only (ALA, 100 mg/kg, i.p.). was treated at 24 h 30 minutes prior irradiation. area including gland evenly with 2 Gy per minute (total dose 18 Gy) using a photon 6-MV...

<b><i>Background:</i></b> This study was conducted to elucidate the role of renal macrophages in development acute kidney injury (AKI) a glycerol (Gly)-induced rhabdomyolysis mouse model. <b><i>Methods:</i></b> The experimental model requires injecting 50% Gly (10 ml/kg) intramuscularly into mice. Control mice were injected tail vein with liposomal vehicle. Liposome-encapsulated clodronate (LEC)-only LEC. Gly-only hind limb. LEC+Gly-treated...

Objectives/Hypothesis Several genes have been reported to be upregulated in human nasal polyps previous genetic analyses. Among these genes, periostin is known overexpressed obtained from aspirin‐sensitive patients. Using periostin‐null mice, we investigated the role of a murine model eosinophilic rhinosinusitis with polyps. Study Design Animal study. Methods Eosinophilic was induced both and wild‐type mice according previously established protocols. In brief, ovalbumin (OVA) used for...

The role of psoralen (PS), a major active component extracted from Psoralea corylifolia L. seed, in renal fibrosis is still unclear. Thus, the objective this study was to evaluate effects PS on development and progression induced by unilateral ureteral obstruction (UUO) mouse model. Mice were divided into four groups: (20 mg/kg, i.g., n = 5), + sham (n UUO 10), 10). intragastrically administered 24 h before continued afterwards for 7 days. All mice killed days post UUO. Severe tubular...

Radiotherapy is currently one of the main treatment modalities for head and neck cancer; however, it also results in severe toxicity to normal tissue, detriment patients. This study aimed investigate whether alpha lipoic acid (ALA) could protect against radiation-induced oral mucositis a rat model.On post-irradiation days 4 7, epithelial layer on mucosa showed pronounced injury (shortening layer) diminished by ALA pretreatment before radiation. Hif-1a expression was significantly induced...

Gastroesophageal reflux is associated with numerous pathologic conditions of the upper aerodigestive tract. Gastric pepsin within contributes to immunologic reactions in tonsil. In this study, we aimed find relationships between and tonsillar hypertrophy.We explored notion whether hypertrophy was due pepsin-mediated gastric tonsil hypertrophy. Fifty-four children 30 adults tonsillitis were recruited before surgical treatment. Blood tissues from each patient harvested for analysis changes...

Vitamin D-binding protein (VDBP) mediates various biological processes in humans. The goal of this study was to investigate whether VDBP gene polymorphisms could predispose Korean women endometriosis. We prospectively enrolled with endometriosis (n = 16) and healthy controls 16). Total serum 25-hydroxyl vitamin D (25(OH)D) concentrations were measured using an Elecsys total kit. Levels bioavailable free 25(OH)D calculated. Concentrations a BP Quantikine ELISA DNA extracted DNeasy blood &...

Peroxisome proliferator-activated receptor-delta (PPAR-δ)-dependent signaling is associated with rapid wound healing in the skin. Here, we investigated therapeutic effects of PPAR-δ-agonist treatment on cardiac post-myocardial infarction (MI) rats. Animals were assigned to following groups: sham-operated control group, left anterior descending coronary artery ligation or MI administration PPAR-δ agonist GW610742 group. (1 mg/kg) was administrated intraperitoneally after operation and...

Radiation therapy is a standard treatment for patients with head and neck cancer. However, radiation exposure to the induces salivary gland (SG) dysfunction. Alpha lipoic acid (ALA) has been reported reduce radiation-induced toxicity in normal tissues. In this study, we investigated effect of ALA on SG Male Sprague-Dawley rats were assigned following groups: control, only (100 mg/kg, intraperitoneally), irradiation only, administration 24 h or 30 min prior irradiation. The area, including...

Objectives The Janus kinase/signal transducer and activator of transcription (JAK–STAT) pathway play a key role in immune modulation, especially the polarization T helper cells. JAK inhibitors reduce inflammation by inhibiting phosphorylation STAT. We investigated whether inhibitor, tofacitinib, can mouse model chronic rhinosinusitis with nasal polyps (CRSwNP). Methods An eosinophilic CRSwNP was induced using 4‐week‐old BALB/c mice. therapeutic effects topical tofacitinib were compared...

Ischemia-reperfusion injury (IRI) is a major cause of acute kidney (AKI) and progression to chronic disease (CKD). However, no effective therapeutic intervention has been established for ischemic AKI. Endothelial progenitor cells (EPCs) have roles in the maintenance vascular integrity repair endothelial damage; they also serve as agents various diseases. Thus, we examined whether EPCs renoprotective effect an IRI mouse model. Mice were assigned sham, EPC, IRI-only, EPC-treated groups....

Abstract The epithelial-to-mesenchymal transition (EMT) is one of mechanisms that induce renal interstitial fibrosis. Understanding EMT in fibrosis has important therapeutic implications for patients with kidney disease. Alpha-lipoic acid (ALA) a natural compound antioxidant properties. Studies ALA are performed acute injury tubular apoptosis, inflammation, and oxidative stress. We investigated the effects on EMT-mediated mice unilateral ureteral obstruction (UUO). UUO developed severe...

Background Chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) is a principally type 2 T helper cell (Th2)-mediated inflammatory disease. Systemic corticosteroids currently represent the most effective treatment for CRSwNP, but their long-term use constrained due to detrimental side effects. Long-term oftopical steroids safe, efficacy often limited. Topical cyclosporine has proven be safe and Th2-mediated diseases such as allergic conjunctivitis. Objective It was hypothesized that...

Dapsone (diaminodiphenyl sulfone, DDS) is currently used to treat leprosy, malaria, dermatitis herpetiformis, and other diseases. It also pneumocystis pneumonia Toxoplasma gondii infection in HIV-positive patients. The most common adverse effect of DDS methemoglobinemia from oxidative stress. Ascorbic acid an antioxidant reducing agent that scavenges the free radicals produced by present study aimed investigate ascorbic treatment induced methemoglobinemia.Male Sprague-Dawley rats were...