Steven M. Jones
A5033476395- Gallbladder and Bile Duct Disorders
- Aortic aneurysm repair treatments
- Vascular Procedures and Complications
- Bacterial biofilms and quorum sensing
- Cellular transport and secretion
- Cardiac, Anesthesia and Surgical Outcomes
- Venous Thromboembolism Diagnosis and Management
- Cancer, Hypoxia, and Metabolism
- Antibiotic Resistance in Bacteria
- Appendicitis Diagnosis and Management
- Cancer therapeutics and mechanisms
- Total Knee Arthroplasty Outcomes
- Peripheral Artery Disease Management
- Gut microbiota and health
- Infectious Aortic and Vascular Conditions
- Aortic Disease and Treatment Approaches
- Orthopaedic implants and arthroplasty
- Pancreatic function and diabetes
- Renal Transplantation Outcomes and Treatments
- Influenza Virus Research Studies
- Animal Ecology and Behavior Studies
- Calcium signaling and nucleotide metabolism
- Ultrasound and Hyperthermia Applications
- Urinary Tract Infections Management
- Wildlife Ecology and Conservation
Children's Hospital of Philadelphia
2023-2025
Corewell Health
2023
University of Calgary
2007-2021
Royal United Hospital
2011-2019
University of Liverpool
2014-2017
Vertex Pharmaceuticals (United States)
2014-2017
Royal Liverpool University Hospital
2014-2017
Bonutti Clinic
2017
John Hunter Hospital
2017
Wirral University Teaching Hospital NHS Foundation Trust
2016
Dynamin guanosine triphosphatases support the scission of clathrin-coated vesicles from plasmalemma during endocytosis. By fluorescence microscopy cultured rat hepatocytes, a green fluorescent protein–dynamin II fusion protein localized with at Golgi complex. A cell-free assay was utilized to demonstrate role dynamin in vesicle formation trans-Golgi. Addition peptide-specific anti-dynamin antibodies mixture inhibited both constitutive exocytic and formation. Immunodepletion proteins also...
Abstract Background The aim was to describe the management of benign gallbladder disease and identify characteristics associated with all-cause 30-day readmissions complications in a prospective population-based cohort. Methods Data were collected on consecutive patients undergoing cholecystectomy acute UK Irish hospitals between 1 March May 2014. Potential explanatory variables influencing analysed by means multilevel, multivariable logistic regression modelling using two-level hierarchical...
Fructooligosaccharides (FOS) are well-known carbohydrates that promote healthy gut microbiota and have been previously demonstrated to enhance levels of Bifidobacterium Lactobacillus. Its bifidogenic properties associated with positive health outcomes such as reduced obesity anti-inflammatory properties, and, therefore, is in use a prebiotic supplement support microbiota. However, the changes age, which may lead differential responses treatments prebiotics other dietary supplements.To...
We have developed a therapeutic for the treatment of anthrax using an affinity-enhanced monoclonal antibody (ETI-204) to protective antigen (PA), which is central cell-binding component exotoxins. ETI-204 administered preexposure by single intravenous injection dose between 2.5 and 10 mg per animal significantly protected rabbits from lethal aerosolized spore challenge ( approximately 60 450 times 50% Bacillus anthracis Ames). Against similar challenge, intramuscularly at 20-mg completely...
Compound 3 is a potent aminobenzimidazole urea with broad-spectrum Gram-positive antibacterial activity resulting from dual inhibition of bacterial gyrase (GyrB) and topoisomerase IV (ParE), it demonstrates efficacy in rodent models infection. Preclinical vitro vivo studies showed that compound covalently labels liver proteins, presumably via formation reactive metabolite, hence presented potential safety liability. The moiety was identified as being potentially responsible for metabolite...
ABSTRACT New drugs to treat drug-resistant tuberculosis are urgently needed. Extensively and probably the totally strains resistant fluoroquinolones like moxifloxacin, which target gyrase A, most people infected with these die within a year. In this study, we found that novel aminobenzimidazole, VXc-486, targets B, potently inhibits multiple drug-sensitive isolates of Mycobacterium in vitro (MICs 0.03 0.30 μg/ml 0.08 5.48 μg/ml, respectively) reduces mycobacterial burdens lungs mice vivo ....
Fenestrated endovascular aneurysm repair (FEVAR) is increasingly being used for juxtarenal aortic aneurysms. The aim of this study was to review long-term results and assess the importance changing stent-graft design on outcomes.This a retrospective all patients who underwent FEVAR within single unit over 12 years (February 2003 December 2015). Kaplan-Meier analysis survival, freedom from target vessel loss, expansion, graft-related endoleak secondary intervention performed. Comparison...
To characterize endogenous molecules and activities of the Golgi complex, proteins in transit were >99% cleared from rat hepatocytes by using cycloheximide (CHX) treatment. The loss resulted condensation cisternae stacks. Isolation a stacked fraction is equally efficient with or without [control (CTL SGF1) (CHX SGF1)]. Electron microscopy morphometric analysis showed that >90% elements could be positively identified as stacks cisternae. Biochemical cis-, medial-, trans-, TGN markers...
Background Marburg virus (MARV), a zoonotic pathogen causing severe hemorrhagic fever in man, has emerged Angola resulting the largest outbreak of (MHF) with highest case fatality rate to date. Methodology/Principal Findings A mobile laboratory unit (MLU) was deployed as part World Health Organization response. Utilizing quantitative real-time PCR assays, this provided specific MARV diagnostics Uige, epicentre outbreak. The MLU operated over period 88 days and tested 620 specimens from 388...
The aims of this prospective population-based cohort study were to identify the patient and hospital characteristics associated with emergency cholecystectomy, influences these in determining variations between hospitals.Data collected for consecutive patients undergoing cholecystectomy acute UK Irish hospitals 1 March May 2014. Potential explanatory variables influencing performance analysed by means multilevel, multivariable logistic regression modelling using a two-level hierarchical...
Background : Staphylococcus aureus is a leading cause of healthcare-associated infection and one the most common pathogens causing serious invasive in neonatal intensive care unit (NICU). Asymptomatic colonization by S. presents significant risk for subsequent colonized infant patient-to-patient transmission. Transmission more virulent strains NICU could increase this vulnerable population. Methods We performed three-year, unit-wide screening Children's Hospital Philadelphia (CHOP) NICU,...
Dissemination of organisms from the gut microbiota is a major contributor to sepsis and critical illness. Patients with cirrhosis are prone systemic infections commonly prescribed carbohydrate lactulose manage hepatic encephalopathy (HE). Commensal metabolism believed reduce pathobiont colonization through short-chain fatty acid production, but its direct effects on pathobionts remain unexplored. Here, we show that consumption unexpectedly selects for mutations in Escherichia coli lactose...
Current evidence suggests that phosphatidylinositol (PI) kinases and transfer protein (PITP) are involved in driving vesicular traffic from yeast mammalian trans-Golgi network (TGN). We have tested the interaction between these cytosolic proteins an assay measures formation of constitutive transport vesicles TGN a hepatocyte cell-free system. This reaction is dependent on novel PI 3-kinase, we now report that, under conditions limiting cytosol, purified 3-kinase PITP functionally cooperate...
We undertook a prospective, randomised study of 135 total knee arthroplasties to determine the most accurate and reliable technique for alignment tibial prosthesis. Tibial resection was guided by either intramedullary or extramedullary jigs.
An 85-kD cytosolic complex (p62cplx), consisting of a 62-kD phosphoprotein (p62) and 25-kD GTPase, has been shown to be essential for the cell-free reconstitution polymeric IgA receptor (pIgA-R)-containing exocytic transport vesicle formation from TGN (Jones, S.M., J.R. Crosby, J. Salamero, K.E. Howell. 1993. Cell Biol. 122:775–788). Here p62cplx is identified as regulatory subunit novel phosphatidylinositol 3–kinase (PI3-kinase). This p62cplx-associated PI3-kinase activity stimulated by...
JNJ-63623872 (2) is a first-in-class, orally bioavailable compound that offers significant potential for the treatment of pandemic and seasonal influenza. Early lead optimization efforts in our 7-azaindole series focused on 1,3-diaminocyclohexyl amide urea substitutions pyrimidine-7-azaindole motif. In this work, we explored two strategies to eliminate observed aldehyde oxidase (AO)-mediated metabolism at 2-position these analogues. Substitution azaindole ring generated somewhat less potent...
Abstract Background The optimal timing of cholecystectomy for patients admitted with acute gallbladder pathology is unclear. Some studies have shown that emergency during the index admission can reduce length hospital stay similar rates conversion to open surgery, complications and mortality compared a ‘delayed’ operation following discharge. Others reported results in higher morbidity, extended increased costs. This study examined cost-effectiveness versus delayed benign disease. Methods...
Proteus mirabilis is a urinary pathogen that can differentiate from swimmer cell into swarmer morphotype and form biofilms on the surfaces of catheters. These block these catheters due to crystals trapped within structures. The effect encrustation biofilm formation structure has not been studied using confocal scanning laser microscopy (CSLM). Therefore, comparison in artificial urine (AU) laboratory media was undertaken. We compared P. AU Luria-Bertani broth CSLM 3D imaging. Biofilms grown...
Previous studies indicated that inhibition of efflux pumps augments tuberculosis therapy. In this study, we used timcodar (formerly VX-853) to determine if pump inhibitor could increase the potency antituberculosis (anti-TB) drugs against Mycobacterium in vitro and vivo combination studies. When alone, weakly inhibited M. growth broth culture (MIC, 19 μg/ml); however, it demonstrated synergism drug with rifampin, bedaquiline, clofazimine but not other anti-TB agents. was cultured host...