A5063028441- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Lymphoma Diagnosis and Treatment
- Viral-associated cancers and disorders
- Kawasaki Disease and Coronary Complications
- Hydrogels: synthesis, properties, applications
- COVID-19 Impact on Reproduction
- Pancreatic and Hepatic Oncology Research
- COVID-19 Clinical Research Studies
- Inflammasome and immune disorders
- Cancer-related molecular mechanisms research
- Advanced Materials and Mechanics
- Cellular Mechanics and Interactions
- Pregnancy and preeclampsia studies
- DNA Repair Mechanisms
- 3D Printing in Biomedical Research
- Polymer Nanocomposites and Properties
- Sphingolipid Metabolism and Signaling
- Inflammatory Bowel Disease
- Scientific Computing and Data Management
- Biomedical Text Mining and Ontologies
- T-cell and Retrovirus Studies
- Coronary Artery Anomalies
- Cytomegalovirus and herpesvirus research
University of Limerick
2019-2025
Abstract Tertiary lymphoid structures play important roles in autoimmune and non-autoimmune conditions. While many of the molecular mechanisms involved tertiary structure formation have been identified, cellular sources temporal spatial relationship remain unknown. Here we use combine single-cell RNA-sequencing, transcriptomics proteomics minor salivary glands patients with Sjogren’s disease Sicca Syndrome, ex-vivo functional studies to construct a map key components function structures. We...
Abstract Pancreatic ductal adenocarcinoma (PDAC) has a poor clinical outlook. Responses to immune checkpoint blockade are suboptimal and much more detailed understanding of the tumor microenvironment is needed if this situation be improved. Here, we characterized tumor-infiltrating T-cell populations in patients with PDAC using cytometry by time flight (CyTOF) single-cell RNA sequencing. T cells were predominant cell subset observed within tumors. Over 30% CD4+ expressed CCR6+CD161+ Th17...
Multisystem inflammatory syndrome in children (MIS-C) is a life-threatening disease occurring several weeks after severe acute respiratory coronavirus 2 (SARS-CoV-2) infection. Deep immune profiling showed MIS-C patients had highly activated neutrophils, classical monocytes and memory CD8+ T-cells, with increased frequencies of B-cell plasmablasts double-negative B-cells. Post treatment samples from the same patients, taken during symptom resolution, identified recovery-associated features...
Background: In biomedical imaging research, experimental biologists generate vast amounts of data that require advanced computational analysis. Breakthroughs in techniques, such as multiplex immunofluorescence tissue imaging, enable detailed proteomic analysis, but most researchers lack the programming and Artificial Intelligence (AI) expertise to leverage these innovations effectively. Methods: Cinco de Bio (CdB) is a web-based, collaborative low-code/no-code modelling execution platform...
Background: A total of 30–40% diffuse large B cell lymphoma (DLBCL) patients will either not respond to the standard therapy or their disease recur. The first-line treatment for DLBCL is rituximab and combination chemotherapy. This involves chemotherapy-induced recruitment tumor-associated macrophages that recognize kill rituximab-opsonized cells. However, we lack insights into factors responsible functionality in tumors. Methods: We have studied effects immunomodulatory lipid...
The Flory-Rehner theoretical description of the free energy in a hydrogel swelling model can be broken into two components: mixing and ionic energy. Conventionally for ionized gels, is characterized as main contributor to and, therefore, assumed negligible. However, this assumption made at equilibrium state ignores dynamics gel swelling. Here, influence on gels quantified through numerical simulations sodium polyacrylate using Mixed Hybrid Finite Element Method. For univalent divalent...
Diffuse large B-cell lymphoma (DLBCL) is the most common malignancy that develops in patients with ataxia-telangiectasia, a cancer-predisposing inherited syndrome characterized by inactivating germline ATM mutations. also frequently mutated sporadic DLBCL. To investigate lymphomagenic mechanisms and lymphoma-specific dependencies underlying defective ATM, we applied ribonucleic acid (RNA)-seq genome-scale loss-offunction clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9...
In classical Hodgkin lymphoma (cHL), responsiveness to immune-checkpoint blockade (ICB) is associated with specific tumor microenvironment (TME) and peripheral blood features. The role of ICB in nodular lymphocyte predominant (NLPHL) not established. To gain insights into its potential NLPHL, we compared TME signatures between HLs using an integrative multiomic analysis. A discovery/validation approach 121 NLPHL 114 cHL patients highlighted >2-fold enrichment programmed cell death-1 (PD-1)...
Mechanotransduction is the initiation of an electrochemical signal as a result mechanical stimuli. It found predominately in biological tissue and its mechanisms are well documented. In gel like tissues body, such articular cartilage intervertebral discs, mechanotransduction regulates matrix building degrading processes keeping adequately hydrated, both with aim minimizing degradation. The responses to loading at constant volume inanimate hydrogel could assist understanding these processes....
Abstract The key role of tertiary lymphoid structures in autoimmune and non-autoimmune conditions has been recently appreciated. While many the molecular mechanisms involved structure (TLS) formation have identified, their cellular sources temporal spatial relationship to each other during development TLS remain unknown. Here we constructed a functional map components minor salivary glands (SG) humans. We confirmed presence an immunofibroblast cell state identified undescribed immunopericyte...
Not available.
<div>Abstract<p>Pancreatic ductal adenocarcinoma (PDAC) has a poor clinical outlook. Responses to immune checkpoint blockade are suboptimal and much more detailed understanding of the tumor microenvironment is needed if this situation be improved. Here, we characterized tumor-infiltrating T-cell populations in patients with PDAC using cytometry by time flight (CyTOF) single-cell RNA sequencing. T cells were predominant cell subset observed within tumors. Over 30%...
Abstract Multisystem inflammatory syndrome in children (MIS-C) is a life-threatening disease occurring several weeks after severe acute respiratory coronavirus 2 (SARS-CoV-2) infection. MIS-C has overlapping clinical features with Kawasaki Disease (KD), rare childhood vasculitis. therapy largely based on KD treatment protocols but whether these diseases share underpinning immunological perturbations unknown. We performed deep immune profiling blood samples from healthy and patients or KD....
Summary COVID-19 placentitis, a rare complication of maternal SARS-CoV-2 infection, only shows detectable virus in the placenta subset cases. We provide deep multi-omic spatial characterisation placentitis from obstetrically complicated infection. found that infected placentas have distinct transcriptional and immunopathological signature. This signature overlaps with virus-negative cases supporting common viral aetiology. An inverse correlation between load disease duration suggests...
Multisystem inflammatory syndrome in children (MIS-C) is a life-threatening disease occurring several weeks after severe acute respiratory coronavirus 2 (SARS-CoV-2) infection. MIS-C has overlapping clinical features with Kawasaki Disease (KD), rare childhood vasculitis, but whether these diseases share underpinning immunological perturbations unknown. Deep immune profiling showed patients had highly activated neutrophils, classical monocytes and memory CD8+ T-cells; increased frequencies of...
<p>Supplementary Figures S1 - S12</p>