A5012054503- Cardiomyopathy and Myosin Studies
- Cardiac electrophysiology and arrhythmias
- Cardiovascular Effects of Exercise
- Ion channel regulation and function
- Sports injuries and prevention
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
- Cardiovascular Function and Risk Factors
- Epigenetics and DNA Methylation
- Genomics and Rare Diseases
- Liver Diseases and Immunity
- Silk-based biomaterials and applications
- Systemic Lupus Erythematosus Research
- Transplantation: Methods and Outcomes
- Receptor Mechanisms and Signaling
- Forensic Toxicology and Drug Analysis
- Congenital heart defects research
- HER2/EGFR in Cancer Research
- Acute Lymphoblastic Leukemia research
- Insect Resistance and Genetics
- Organ Transplantation Techniques and Outcomes
- Colorectal Cancer Treatments and Studies
- Neurogenetic and Muscular Disorders Research
- DNA Repair Mechanisms
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
Translational Research Institute
2024
Zhuhai People's Hospital
2022-2024
Sun Yat-sen University
2019-2022
Fifth Affiliated Hospital of Sun Yat-sen University
2019-2022
Dongguan People’s Hospital
2022
Guangdong Medical College
2022
Jinan University
2022
Jiangsu University
2018
Institute of Life Sciences
2018
Johns Hopkins Medicine
2010
Abstract Excessive cardiac fibrosis and inflammation aberrantly contribute to the progressive pathogenesis of arrhythmogenic cardiomyopathy (ACM). Whether sodium‐glucose cotransporter‐2 inhibitor (SGLT2i), as a new hypoglycemic drug, benefits ACM remains unclear. Cardiomyocyte‐specific Dsg2 exon‐11 knockout wild‐type (WT) littermate mice were used animal model controls, respectively. Mice administered by gavage with either SGLT2i dapagliflozin (DAPA, 1 mg/kg/day) or vehicle alone for 8...
Arrhythmogenic cardiomyopathy (ACM), a fatal heart disease characterized by fibroadipocytic replacement of cardiac myocytes, accounts for 20% sudden death and lacks effective treatment. It is often caused mutations in desmosome proteins, with Desmoglein-2 (DSG2) as common etiology. However, the mechanism underlying accumulation fibrofatty ACM remains unknown, which impedes development curative Here we investigated fat mouse model induced cardiac-specific knockout Dsg2 (CS-Dsg2−/−). Heart...
Abstract Background The left ventricular noncompaction cardiomyopathy (LVNC) is a rare subtype of associated with high risk heart failure (HF), thromboembolism, arrhythmia, and sudden cardiac death. Methods proband overlap phenotypes LVNC hypertrophic (HCM) complicates atrial fibrillation (AF), tachycardia (VT), HF due to the diffuse myocardial lesion, which were diagnosed by electrocardiogram, echocardiogram magnetic resonance imaging. Peripheral blood was collected from his relatives. DNA...
The whole exome sequencing (WES) with targeted gene analysis is an effective diagnostic tool for cardiomyopathy. early-onset sudden cardiac death (SCD) was commonly associated dilated cardiomyopathy (DCM) induced by pathogenic genetic mutations.In a Chinese Han family, the patient of 24 years old occurred and DCM died SCD ICD storms repetitive ventricular tachycardia/fibrillation (VT/F). Genomic DNA samples peripheral blood were conducted WES Sanger sequence. Then, we performed...
Background Colorectal cancer patients harbouring KRAS mutations in codon 12 or 13 do not benefit from current anti-epidermal growth factor receptor (EGFR) monoclonal antibody therapies. Efficient and robust methods are therefore required for routine clinical testing of mutation status. Aims To evaluate a novel multiplex assay the rapid detection common formalin-fixed paraffin-embedded (FFPE) tissues. Methods Genomic DNA was amplified by PCR using primers targeting 12/13 region an internal...
Determination of NPM1 mutation status has become essential for the molecular classification acute myeloid leukemias (AML). Methods with high clinical sensitivity and specificity adapted to laboratory workflow are required diagnosis, prognosis, monitoring AML normal karyotype. We report here development evaluation a novel, streamlined, RNA-based assay rapid multiplex detection common mutations in 96-well format. Using synthetic transcripts total RNA from leukemic cell lines, we show that can...
Abstract Primary biliary cholangitis (PBC) and autoimmune hepatitis (AIH) are diseases that target hepatocytes bile duct cells, respectively. Despite their shared nature, the differences in immunologic characteristics between them remain largely unexplored. This study seeks to elucidate unique immunological profiles of PBC AIH identify key differences. We comprehensively analyzed various T cell subsets receptor expression a cohort 45 patients, including 27 18 cases. Both exhibited exhaustion...
Abstract Background Arrhythmogenic right ventricular cardiomyopathy/dysplasia (ARVC/D) is associated with arrhythmia, heart failure (HF), and sudden death. Thromboembolism also an important serious complication of ARVC/D. However, the etiology ARVC/D thromboembolism their association genetic mutations are unclear. Methods Genomic DNA samples peripheral blood were conducted for whole-exome sequencing (WES) Sanger in family. Then, we performed bioinformatics analysis genes susceptible to...
Abstract Background Brugada syndrome (Brs) and long QT (LQTs) are the most observed “inherited primary arrhythmia syndromes” “channelopathies”, which lead to sudden cardiac death. Methods Detailed clinical information of Brs LQTs patients was collected. Genomic DNA samples peripheral blood were conducted for whole-exome sequencing on Illumina HiSeq 2000 platform. Then, we performed bioinformatics analysis 200 genes susceptible arrhythmias cardiomyopathies. Protein interaction transcriptomic...
Long QT syndrome (LQTS) is a life-threatening inherited channelopathy, and prolonged intervals easily trigger malignant arrhythmias, especially torsades de pointes ventricular fibrillation.The proband with overlapped phenotypes of LQTS sinoatrial node dysfunction underwent some necessary examinations, including echocardiography, electrocardiogram (ECG), Holter monitoring. Next, whole-exome sequencing was performed, candidate genes were validated by Sanger sequencing. RNA secondary structure...
DNA repair by the nonhomologous end joining (NHEJ) pathway promotes tumor recurrence after chemotherapy and radiotherapy. Discovery of rapid high-throughput techniques to screen for an effective NHEJ inhibitor drug is imperative suppression during treatment. However, traditional screening methods are too cumbersome meet current need. Zebrafish ideal model due specificity its early embryonic development similarity cell generation. By exploiting high frequency in development, we established a...
In order to study the mechanism of programmed cell death (PCD) in silkworm Bombyx mori, we employed suppressive subtractive hybridization screen associated genes. this study, a novel gene was isolated from B. which may be involved PCD as analyzed by bioinformatics and it named DRP (death-related protein) gene. RT-PCR analysis showed prokaryotic expression. Through death-related protein different tissues developmental stage distributed condition It widely expressed various mainly testis,...
Abstract Introduction Long QT syndrome (LQTS) increases the risk of life‐threatening arrhythmia in young individuals with structurally normal hearts. Sixteen genes such as KCNQ1, KCNH2, and SCN5A have been reported for association LQTS. Case presentation We identified compound heterozygous mutations KCNQ1 gene at c. G527A (p.W176X) c.G1765A (p.G589S) predicted “damaging.” The in‐silico analysis showed that when compared to characteristics mRNA protein wild‐type c.G527A mutation was...
Background: Transplant vasculopathy (TV) is a hallmark of chronic allograft rejection the primary cause loss after organ transplantation. Because multiple mechanisms are involved in TV pathogenesis, effective therapy for it remains deficiency. This study aimed to identify role triptolide, which has wide spectrum immuno-suppressive activities, inhibiting development.Methods: Mice aortic transplant models were constructed and divided into triptolide-treatment control group. The pathological...
Objective: A complex familial arrhythmia syndrome was identified in a Chinese Han family, characterized by sick sinus syndrome, progressive conduction block, atrial fibrillation, standstill and Brugada syndrome. The underlying mechanism associated with the genetic mutation investigated. Methods: Genetic analysis conducted for probands coding splicing regions of genes susceptible to cardiac diseases. stable cell lines overexpressing wild-type (WT) or mutant SCN5A gene were generated HEK293T...