Cervical microbial communities serve a crucial role in the persistence and development of oncogenic human papilloma virus (HPV) infections. In present study, authors hypothesised that disturbed heterogeneity flora was associated with HPV-induced carcinogenesis. Swabs cervical microbiota were collected from 250 women 16S ribosomal DNA sequenced using high throughput assay. The swabs grouped according to community state types (CSTs) as follows: Healthy swabs; taken low-grade squamous...

Background/Objectives: Endometriosis is a painful chronic condition in which the endometrium grows outside uterus. The epithelial–mesenchymal transition (EMT) critical to endometriosis progression, where cells lose epithelial traits and gain invasiveness. Methods: This study investigates effects of phoenixin-14 (PNX-14), neuropeptide found at reduced levels patients, on expression two molecular EMT markers, CDH1 (E-cadherin) THBS2 (thrombospondin 2), as well cell viability...

The present study aimed to investigate the effects of Carnobacterium divergens AS7 bacteriocin (divercin AS7) on growth performance, digestibility, fermentation processes, selected microbial populations, and histomorphology in broiler chickens challenged with a mixture 3 Clostridium perfringens isolates. In total, 480 one-day-old male Ross 308 chicks were randomly assigned 4 experimental groups (12 replicate pens 10 birds per treatment). diets either nonsupplemented or supplemented...

Milky sap isolated from Chelidonium majus L. (Greater Celandine) serves as a rich source of various biologically active substances such alkaloids, flavonoids and phenolic acids. Previous research showed that the activity Ch. milky may depend also on presence proteins. The goal this study was to evaluate biological effect two nucleases milk sap, CMN1 20 kDa CMN2 36 kDa, HeLa CHO tumour cell lines. Both studied together with other proteins in plant are involved stress defence reactions against...

Aim. To assess the role of Th1 and Th2 cellular response in etiology endometriosis observed a rat model, with use RESAN immunomodulator. Materials Methods. A comparative analysis cytokines blood serum typical (TNF- α INF- γ ) (IL-4, IL-6, IL-10) cell groups rats, which preparation was used as prophylaxis (Gr. I) or treatment II) endometriosis. Results. The results indicated an increase level by comparing second third stages experiment group rats decrease IL-4 IL-10 between III IV stages....

Small integral membrane protein 20/phoenixin (SMIM20/PNX) and its receptor GPR173 (G Protein-Coupled Receptor 173) play a role in the regulation of hypothalamic-pituitary-gonadal axis (HPG). The aim study was to determine PNX, FSH, LH, 17β-estradiol association women with endometriosis, expression SMIM20/PNX signaling via GPR173. Serum concentrations were measured by enzyme electrochemiluminescence immunoassay. eutopic ectopic endometrium assessed qPCR immunohistochemistry. Reduced PNX...

Current techniques of in vitro cell cultures are able to mimic the vivo environment only a limited extent, as they enable cells grow two dimensions. Therefore culture approaches should rely on scaffolds that provide support comparable extracellular matrix. Here we demonstrate advantages novel nanostructured three-dimensional grids fabricated using electro-spinning technique, for neoplastic cells. The results study show fibers allow dynamic growth HeLa cells, which form multi-layer structures...

We present here—designed, manufactured, and tested by our research team—the Ti-alloy prototype of the multispiked connecting scaffold (MSC-Scaffold) interfacing components resurfacing arthroplasty (RA) endoprostheses with bone. The spikes MSC-Scaffold mimic interdigitations articular subchondral bone, which is natural biostructure cartilage periarticular trabecular To enhance osteoinduction/osteointegration potential MSC-Scaffold, attempts to modify its bone contacting surfaces process...

Corydalis cava Schweigg. & Koerte, the plant of numerous pharmacological activities, together with studied earlier by our group Chelidonium majus L. (Greater Celandine), belong to family Papaveraceae. The grows in Central and South Europe produces sizeable subterraneous tubers, empty inside, which are extremely resistant various pathogen attacks. sp. tubers a rich source many biologically active substances, extensive use European Asian folk medicine. They have analgetic, sedating, narcotic,...

Abstract Background Metastasis is a common feature of many advanced stage cancers and metastatic spread thought to be responsible for cancer progression. Most cells are localized in the primary tumor only small population circulating (CTC) has potential. CTC amount reflects aggressiveness tumors, therefore their detection can used determine prognosis treatment patients. The aim this study was evaluate human chorionic gonadotropin beta subunit (CGB) gonadoliberin type 1 (GNRH1) expression as...

Endometriosis is a gynecological disease defined by the presence of endometrial tissue outside uterus. To date, effective treatment this still based on invasive surgery or laparoscopy. Chelidonium majus L. (Papaveraceae) belongs to medicinal, latex-bearing plants. Extracts from plant are rich source pharmacologically active agents. Protoberberine compounds derived C. possess anticancer and antiproliferative activities. In present study rat model endometriosis, we investigated influence...

Endometrial carcinoma (EC) is the most common type of gynecological malignancy. Studies have demonstrated that insulin growth factor (IGF) pathway implicated in development endometrial tumors and serum levels IGF‑1 are affected by estrogen. Most EC cells with high microsatellite instability (MSI‑H) accumulate mutations at a sequence gene. The present study investigated CA repeat polymorphism P1 promoter region gene among Caucasian females hyperplasia, healthy control subjects, whose blood...

Endometriosis, the presence of ectopic endometrium, has an unclear etiology and is commonly associated with endocrine, genetic, immunological imbalance. This study determined whether immunomodulation by RESAN vaccine could alter potentially pathogenic gene expression profiles in cells eutopic endometrium animal model endometriosis. Preventing these changes inhibit early development illness support success surgical treatment. Wistar rats were divided into three groups: prophylaxis (vaccinated...

Mallory's triple staining is a histochemical technique used mainly for analysing connective tissues and glands other tissues. We have described the differences in nuclear between eutopic ectopic endometrium as well endometrial hyperplasia adenocarcinoma using method. The ultrastructural been detected. In normal hyperplastic presence of stromal cell nuclei with an increased affinity to aniline blue has observed. disappeared after digestion RNase. cases endometriosis, independently types,...

High oncogenic risk human papillomaviruses (HPVs) are closely associated with cancer of the cervix. However, HPV infection alone may not be sufficient to cause cervical cancer, and other factors or cofactors have a cumulative effect on progression from cancer. The present study investigates cytosine‑adenine (CA) repeat polymorphism in P1 promoter region insulin‑like growth factor‑1 (IGF‑1) gene among precancerous patients healthy control females. association between these polymorphisms,...

The aim of the present study was to examine whether effects endometriosis-targeted photodynamic therapy (PDT), dependent on 5-aminolevulinic acid (ALA), rely presence P-glycoprotein (P-gp), which is regarded as constituting one causes multidrug resistance phenomenon.The significance undertaken studies reflects complete absence reports related modulation P-gp activity and efficacy PDT in patients with endometriosis.Tissue samples normal endometria were obtained from eight women after...

Endometriosis is one of the most common gynecologic diseases in women reproductive age. The pathophysiology endometriosis still not fully understood. Phoenixin (PNX-14) a newly discovered neuropeptide that regulates hypothalamo-pituitary-gonadal (HPG) axis and functions. Recently, we reported PNX-14, its precursor protein receptor were expressed human endometrium. Moreover, PNX-14 serum levels reduced. This study aimed to evaluate vitro biological functions physiological concentrations on...

Despite progress in medicine, appropriate diagnosis and treatment of endometriosis poses a serious problem. For this reason, in-vitro experiments were performed on potential method employing photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA).The exogenous application ALA induces the accumulation protoporphyrin IX, natural effective photosensitizer used therapy.To end primary epithelial cells isolated from endometriotic foci, preincubated with various concentrations, then exposed to...

ENWEndNote BIBJabRef, Mendeley RISPapers, Reference Manager, RefWorks, Zotero AMA Oleś D, Szczepankiewicz A, Wołuń-Cholewa M, et al. Original paperDoes concentration influence viability of the bronchial epithelial cell line chronically exposed to antiasthmatic drugs?. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii. 2012;29(5):363-368. doi:10.5114/pdia.2012.31489. APA Oleś, D., Szczepankiewicz, A., Wołuń-Cholewa, M., Butowska, W., Sobkowiak, P., & Kycler, Z....