A5005343602- Saffron Plant Research Studies
- Reproductive Biology and Fertility
- Phytochemical and Pharmacological Studies
- Metabolism and Genetic Disorders
- Sirtuins and Resveratrol in Medicine
- Flavonoids in Medical Research
- Hemoglobinopathies and Related Disorders
- Ovarian function and disorders
- Glycosylation and Glycoproteins Research
- Bone health and treatments
- Blood properties and coagulation
- Iron Metabolism and Disorders
- Estrogen and related hormone effects
- Carbohydrate Chemistry and Synthesis
- PI3K/AKT/mTOR signaling in cancer
- Cancer, Lipids, and Metabolism
- Bee Products Chemical Analysis
- Hedgehog Signaling Pathway Studies
- Cytokine Signaling Pathways and Interactions
- Cancer-related Molecular Pathways
- Hemoglobin structure and function
- Therapeutic Uses of Natural Elements
- Retinal Diseases and Treatments
- Protein Interaction Studies and Fluorescence Analysis
- Retinal Imaging and Analysis
University of L'Aquila
2012-2023
University of Naples Federico II
2002-2020
San Salvatore Hospital
2014
Magna Graecia University
2005
Sapienza University of Rome
1991
SUMMARY ANSWERSIRT1 plays a pivotal role in the adaptive response of mouse germinal vesicle (GV) oocytes to OS and promotes signalling cascade leading up-regulation MnSod gene.
Crocus sativus L. extracts (saffron) are rich in carotenoids. Preclinical studies have shown that dietary intake of carotenoids has antitumor effects suggesting their potential preventive and/or therapeutic roles. We recently reported saffron (SE) and crocin (CR) exhibit anticancer activity by promoting cell cycle arrest prostate cancer (PCa) cells. It also been demonstrated crocetin esters produced after SE gastrointestinal digestion CR hydrolysis. The aim the present report was to...
Bisphosphonates (BPs) are an emerging class of drugs mostly used in the palliative care cancer patients. We investigated vitro activity most potent antiresorptive BP, zoledronic acid (ZOL), on growth and survival three human pancreatic (PC) cell lines (BxPC-3, CFPAC-1 PANC-1). Pancreatic frequently has a dysregulated p21ras pathway therefore appears to be suitable target for BPs that interfere with prenylation small GTP-binding proteins such as p21ras. found ZOL induces inhibition...
In this study, we investigated the antiproliferative effects of saffron extract (SE) and its major constituent crocin on 5 different malignant 2 nonmalignant prostate cancer cell lines. Using high performance liquid chromatography (HPLC), purity content were determined. All cells incubated with concentrations SE or for 48 h. Cell cycle apoptosis also evaluated. a time- concentration-dependent manner, both reduced proliferation in all lines IC50 values ranging between 0.4 4 mg/ml 0.26 0.95...
Cancer therapies are associated with increased infertility risk due to accelerated reproductive aging. Oxidative stress (OS) is a potential mechanism behind ovarian toxicity by cyclophosphamide (CPM), the most ovotoxic anticancer drug. An important sensor of OS SIRT1, NAD + ‐dependent deacetylase which regulates cellular defence and cell fate. This study investigated whether natural carotenoid crocetin synthetic compound AS101 protect ovary against CPM modulating SIRT1 mitochondrial markers....
Polycystic ovary syndrome (PCOS) is a complex metabolic disorder associated with female infertility. Based on energy and antioxidant regulatory functions of carnitines, we investigated whether acyl-L-carnitines improve PCOS phenotype in mouse model induced by dehydroepiandrosterone (DHEA). CD1 mice received DHEA for 20 days along two different carnitine formulations: one containing L-carnitine (LC) acetyl-L-carnitine (ALC), the other also propionyl-L-carnitine (PLC). We evaluated estrous...
One of the major obstacles in treatment hormone-refractory prostate cancer (HRPC) is development chemoresistant tumors. The aim this study to evaluate role azacitidine as chemosensitizing agent association with docetaxel (DTX) and cisplatin using two models aggressive cancer, 22rv1, PC3 cell lines. Azacitidine shows antiproliferative effects associated increased proportion cells G0/G1 evident apoptosis 22rv1 G2/M phase absence acute killing cells. In vivo, (0.8 mg/kg i.p.) reduced tumor...
Advanced glycation end-products (AGEs) are involved in the pathogenesis and consequences of polycystic ovary syndrome (PCOS), a complex metabolic disorder associated with female infertility. The most powerful AGE precursor is methylglyoxal (MG), byproduct glycolysis, that detoxified by glyoxalase system. By using PCOS mouse model induced administration dehydroepiandrosterone (DHEA), we investigated whether MG-dependent glycative stress contributes to ovarian phenotype explored changes...
Zoledronic acid (ZOL) has proved activity in bone metastases from prostate cancer through inhibition of mevalonate pathway and prenylation intracellular proteins. We have reported that ZOL synergizes with R115777 farnesyltransferase inhibitor (FTI, Zarnestra) inducing apoptosis growth on epidermoid cells. Here, we studied the effects combination these agents adenocarcinoma models and, specifically, androgen-independent (PC3 DU145) -dependent (LNCaP) cell lines. found were synergistic both...
<b><i>Background:</i></b> Hypertension is associated with endothelial dysfunction characterized by decreased vasorelaxation. Crocetin, a bioactive compound of saffron, exhibits favorable cardiovascular properties. We analyze the vasomodulatory effects crocetin in hypertension. <b><i>Methods:</i></b> Myographical experiments were performed to compare relaxation induced acetylcholine (ACH) on aortic rings from normotensive (Wistar) and...
Background: Endothelial dysfunction, characterized by an enhancement in vasoconstriction, is clearly associated with hypertension. Saffron (Crocus sativus L.) bioactive compounds have been recognized to hypotensive properties. Recently, we reported that crocetin exhibits potent vasodilator effects on isolated aortic rings from hypertensive rats. In this work, aimed analyze the anticontractile ability of or esters pool (crocins) saffron. Thus, studied saffron carotenoids endothelium-dependent...
Abstract We have previously reported that interferon‐α (IFNα) induces apoptosis and EGF can antagonize this effect in human epidermoid cancer KB cells. Since occurs together with cytoskeleton reorganization we evaluated if IFNα could modulate cell remodeling our experimental conditions. found 48 h 1,000 IU/ml induced structural of stress fibers membrane delocalization partial capping the actin severing protein gelsolin. The transfection cells both a wild type (WT) or C‐terminal truncated...
The aim of this study was to evaluate retinal and choriocapillaris (CC) vessel density, using optical coherence tomography angiography (OCTA), in the macular region at baseline after three once-monthly intravitreal injections bevacizumab (loading phase) patients affected by exudative age-related degeneration (AMD). A total 38 eyes with AMD (16 women, 22 men; mean age 72.47 ± 7.46 years) were included study. We evaluated density different areas (whole image, fovea parafovea) superficial...
Abstract Interferon α (IFNα) induces an EGF‐Ras→Raf‐1→Erk dependent survival pathway counteracting apoptosis induced by the cytokine. In this paper we have evaluated effects of combination between farnesyl‐transferase inhibitor (FTI) R115777 and IFNα on growth inhibition cancer cells. Simultaneous exposure to produced synergistic both antiproliferative proapoptotic effects. these experimental conditions, completely antagonized increased activity Ras Erk‐1/2 strongly reduced Akt activity....