A5100766058- HIV Research and Treatment
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- HIV/AIDS drug development and treatment
- Plant Virus Research Studies
- Viral gastroenteritis research and epidemiology
- HIV/AIDS Research and Interventions
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Mosquito-borne diseases and control
- Cancer Research and Treatments
- Vector-Borne Animal Diseases
- Hepatitis C virus research
- Viral Infections and Vectors
- Evaluation Methods in Various Fields
- Cytomegalovirus and herpesvirus research
- Plant Pathogenic Bacteria Studies
- HIV, Drug Use, Sexual Risk
- Virology and Viral Diseases
- Immunotherapy and Immune Responses
- Hepatitis B Virus Studies
- Tuberculosis Research and Epidemiology
- Infections and bacterial resistance
- Neurogenesis and neuroplasticity mechanisms
- Yersinia bacterium, plague, ectoparasites research
Capital Medical University
2024
Beijing Anding Hospital
2024
Binzhou University
2024
Qingdao University
2000-2024
Binzhou Medical University
2024
South China Agricultural University
2023
Shandong Agricultural University
2020
Harbin Veterinary Research Institute
2014-2018
Chinese Academy of Agricultural Sciences
2018
China Agricultural University
2007-2017
MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression posttranscriptionally through silencing or degrading their targets, thus playing important roles in the immune response. However, role of miRNAs host response against infectious bursal disease virus (IBDV) infection is not clear. In this study, we show a series was significantly altered DF-1 cells after IBDV infection. We found miRNA gga-miR-130b inhibited replication via targeting specific sequence segment A and...
Glycosylated Gag (glycoGag) is an accessory protein expressed by most gammaretroviruses, including murine leukemia virus (MLV). MLV glycoGag not only enhances replication and disease progression but also increases human immunodeficiency type 1 (HIV-1) infectivity as Nef does. Recently, SERINC5 (Ser5) was identified the target for Nef, Nef-like activity has been attributed to Ser5 antagonism. Here, we investigated how antagonizes using glycoMA proteins. We confirm previous observations that...
Abstract Glioblastoma (GBM) is the most prevalent and lethal type of primary malignant brain tumour. Recent studies suggest that discovery human cytomegalovirus (HCMV)-encoded microRNAs (miRNAs) might play a role in pathogenesis diseases, including GBM. In this study, we aimed to analyse expression function HCMV-encoded miRNAs We found miR-UL112-3p was significantly elevated GBM, its levels were highly associated with glioma size, differentiation, WHO stage overall disease-free survival...
Abstract: Any global strategy for controlling the human immunodeficiency virus (HIV) epidemic is likely to rely heavily on immunization of infants and children. Given well‐documented differences in children's responses traditional vaccines, we initiated this study extend our findings DNA vaccination adult chimpanzees immunologically immature infant chimpanzees. Our were consistent with previous work adults as observed that vaccines used here both well tolerated immunogenic within weeks...
Abstract Porcine reproductive and respiratory syndrome (PRRS) caused by PRRS virus (PRRSV) is one of the most severe swine diseases that affects almost all swine-breeding countries. Nonstructural protein 2 (NSP2) important viral proteins in PRRSV life cycle. Our previous study showed NSP2 could induce formation aggresomes. In this we explored effects aggresome on cells found autophagy, which depended to activate aggrephagy. The transmembrane tail domains contributed aggrephagy cellular...
To display antigenic protein on the surface of virus-like particles (VLPs) presents a potentially powerful strategy for vaccine development. We genetically engineered major capsid VP1 foot-and-mouth disease virus (FMDV) into predominant epitope C HBV core gene to yield chimeric core–VP1 VLP. The VLP was successfully expressed in HeLa cells transfected with DNA construct. Compared regular construct, immunization induced significantly higher levels antigen-specific IgG production, T cell...
Glioblastoma multiforme (GBM) is a highly aggressive primary brain tumor characterized by poor prognosis and lack of effective treatments. In recent years, peptide vaccines that use sequences based on tumor-specific or tumor-associated antigens to activate immune responses against cells have emerged as new therapeutic strategy. this study, we developed novel polypeptide vaccine targeting the antigen Fibrinogen-Like Protein 2 (FGL2), whose dominant epitope was tandemly linked C-terminus...
Porcine reproductive and respiratory syndrome virus (PRRSV) has caused huge economic losses for the global pig industry, but its origins evolution remain a mystery. In 2018, genome sequences of seven arteriviruses isolated from rodents were determined, here we publish new analysis showing that they may be ancestors PRRSV. The sequence similarity these viruses to PRRSV was ~60%, with shared organization other characteristics, such as slippery C-rich motifs in nsp2, transactivated protein...
Vaccine development strategies have often utilized recombinant envelope glycoproteins which usually generate strong humoral immune responses but do not cytotoxic T lymphyocytes (CTL). A recent novel experimental vaccination approach involves the technology known as nucleic acid immunization in DNA plasmids expressing a gene of interest is injected intramuscularly animals. These expressed proteins then are presented to system with subsequent antibody and cellular (particularly CTL) responses....
A new HIV-1 circulating recombinant form (CRF), CRF33_01B, has been identified in Malaysia. Concurrently we found a unique (URF), that is, the isolate 06MYKLD46, Kuala Lumpur, It is composed of B or Thai variant subtype (B') and CRF01_AE. Here, determined near full-length genome 06MYKLD46 performed detailed phylogenetic bootscanning analyses to characterize its mosaic composition further confirm assignments. Although majority CRF01_AE, three short fragments B′ inserted along genome. These...
An epidemiologically linked HIV-1-infected cohort, in which a nonprogressor donor infected two recipients who progressed to AIDS, was examined. Sequence analysis, over time, of HIV-1 vpr gene quasispecies from uncultured peripheral blood cells revealed an insertion arginine at position 90 altering highly conserved C-terminal motif, believed play role Vpr nuclear targeting. Full genome analysis each patient showed no defects other regions, implying that the mutational selection unique gene. A...
The Asian HIV epidemic appears to be complex, characterized by the prevalence of multiple subtypes and circulating recombinant forms with gradual replacement pure HIV-1 in several geographical regions. main objectives present study are identify analyse full-length viral genomes three unique (URFs); isolates 07MYKLD47, 07MYKLD48 07MYKLD49 from Malaysia. Long-range polymerase chain reaction (PCR) amplification seven overlapping reading frames was used derive near genomes. Detailed phylogenetic...
In the present study, role of CLDN4 in PRRSV infection was studied. The results showed that blocked absorption into cells and restricted extracellular viral particles via interaction between small loop, ECL2, surface protein GP3. GP3 found to downregulate through ubiquitination transcription factor SP1 facilitate entry. mechanism by which highly pathogenic infects epithelium is suggested. A novel function regulating gene discovered. Moreover, ECL2 could block infection, as well neutralizing...
India has experienced multiple introductions of diverse HIV-1 subtypes A, B, C, and E, along with subtype B HIV-2 between the 1980s early 1990s. In this study, we have carried out a molecular investigation 21 heterosexually vertically acquired HIV-infected individuals from New Bombay area, who tested positive for by commercial enzyme-linked immunosorbent assay (ELISA) Western blot assay. We sequenced proviral DNA segments uncultured PBMCs in hypervariable env V(3) region (286 bp) full-length...