Abstract Human blood is conventionally considered sterile but recent studies suggest the presence of a microbiome in healthy individuals. Here we characterized DNA signatures microbes 9,770 individuals using sequencing data from multiple cohorts. After filtering for contaminants, identified 117 microbial species blood, some which had replication. They were primarily commensals associated with gut ( n = 40), mouth 32) and genitourinary tract 18), distinct pathogens detected hospital cultures....

The host response to the low pathogenic avian influenza (LPAI) H5N2, H5N3 and H9N2 viruses were examined in A549, MDCK, CEF cells using a systems-based approach. H5N2 replicated efficiently A549 MDCK cells, while virus least these cell types. However, all LPAI exhibited similar higher replication efficiencies cells. A comparison of responses H1N1/WSN passage pH1N1 clinical isolates was performed subtypes robust induction Type I III interferon (IFN) expression, sustained STAT1 activation from...

Diastereoselective anti-aminooxygenation and anti-diamination of alkenes with amidines were enabled by hypervalent iodine(III) reagents such as PhI(OCOR)2 PhI(NMs2)2, respectively. The present transformation offers diastereochemically pure dihydroimidazoles divergently from E- Z-alkenes.

Primary pulmonary synovial sarcoma (PPSS) is an extremely rare malignancy that presents diagnostic challenges due to its overlapping clinical features with other lung diseases. We present the case of a 53-year-old woman history bronchial artery malformation and right organizing pneumonia, who experienced eight-month intermittent cough sputum. Despite multiple imaging studies biopsies at different hospitals, definitive diagnosis was not reached until CT scan chest revealed enlarged mass in...

Abstract Background Respiratory syncytial virus (RSV) is an important cause of lower respiratory tract infection in young children. The degree disease severity determined by the host response to infection. Lung macrophages play early role and we have used a systems-based approach examine RSV-infected lung-derived macrophage cells. Results cells could be efficiently infected (>95%) with RSV vitro , expression several structural proteins detected. Although failed detect significant levels...

Mandibular angle reduction is a popular and efficient procedure widely used to alter the facial contour. The primary surgical instruments, reciprocating saw round burr, employed in surgery have common feature: operating at high-speed. Generally, inexperienced surgeons need long-time practice learn how minimize risks caused by uncontrolled contacts cutting motions manipulation of instruments with high-speed reciprocation or rotation. A virtual reality-based simulator for mandibular was...

Nosocomial infections, including Clostridium difficile infection (CDI), and their fatality rates have increased in the past few decades. Despite emerging molecular diagnostic technologies with rapid, accurate outcomes, nucleic acid extraction from stool samples remains first limiting step before downstream applications. Commercial kits greatly decrease labor time requirements, also provide preparations higher quality purity for enzyme digestion analysis or genotyping. The magnetic bead based...

Osseointegration commences with foreign body inflammation upon implant placement, where macrophages play a crucial role in the immune response. Subsequently, during intermediate and late stages of osseointegration, mesenchymal stem cells (MSCs) migrate initiate their osteogenic functions, while support MSCs osteogenesis. The utilization ferroelectric P(VDF-TrFE) covered ITO planar microelectrodes facilitated simulation various surface charge to investigate effects on MSCs’ differentiation...

The poxviruses are large, linear, double-stranded DNA viruses about 130 to 230 kbp, that have an animal origin and evolved infect a wide host range. Variola virus (VARV), the causative agent of smallpox, is poxvirus infects only humans, but other such as monkey cowpox (CPXV) crossed over from animals humans. Therefore understanding biology can devise antiviral strategies prevent these human infections. In this study we used system-based approach examine responses three orthopoxviruses, CPXV,...

The prevalence of osteoporosis and low bone mass is steadily rising each year. Low body weight commonly linked to diminished serves as a robust predictor osteoporosis. Nonetheless, the connection between index (BMI), mineral density, lipid profiles among elderly remains elusive.To examine association BMI mass, explore correlation delve into interplay metabolism health.The study included 520 patients aged ≥ 65 years (178 men 342 women). Age, sex, weight, height were recorded. Femoral neck...

Influenza A virus (IAV) is a major public health concern, being responsible for the death of approximately half million people each year. Zoonotic transmissions from swine and avian origin have occurred in past, can potentially lead to emgergence new IAV stains future pandemics. Pulmonary macrophages been implicated disease severity lower airway, understanding host response of infected with influenza viruses should provide therapeutic strategies. We used systems-based approach...

Abstract Human blood is conventionally considered sterile. Recent studies have challenged this, suggesting the presence of a microbiome in healthy humans. We present largest investigation to date microbes blood, based on shotgun sequencing libraries from 9,770 subjects. Leveraging availability data multiple cohorts, we stringently filtered for laboratory contaminants identify 117 microbial species detected sampled individuals, some which had signatures DNA replication. These primarily...

Influenza remains one of the most prevalent viruses circulating amongst humans and has resulted in several pandemics. The prevention control H3N2 influenza is complicated by its propensity for evolution, which leads to vaccine mismatch reduced efficacies. This study employed strategy serial passaging compare evolution human seasonal strain A/Singapore/G2-31.1/2014(H3N2) MDCK-SIAT1 versus primary chick embryo fibroblast (CEF) cells. Genetic analysis HA, NS1, NA, PB1 gene segments Sanger...

Although the influenza A virus H7N9 subtype circulates within several avian species, it can also infect humans with a severe disease outcome. To better understand biology of we examined host response to infection in and human cells. In this study used A/Anhui/1/2013 strain, which was isolated during first wave epidemic. The virus-infected both (Airway Epithelial cells) (Chick Embryo Fibroblast) cells, each infected transcriptome bioinformatic tools compared other representative subtypes....

Abstract Human blood is conventionally considered sterile. Recent studies have challenged this, suggesting the presence of a microbiome in healthy humans. We present largest investigation to date microbes blood, based on shotgun sequencing libraries from 9,770 subjects. Leveraging availability data multiple cohorts, we stringently filtered for laboratory contaminants identify 117 microbial species detected sampled individuals, some which had signatures DNA replication. These primarily...

Coronary artery fistula (CAF) is uncommon but remains the most frequent hemodynamically significant congenital coronary anomaly.1 The majority of single and drains into right heart, only 3.5% left ventricle.2 A large requires closure to prevent complications such as myocardial ischemia resulting from steal, congestive heart failure, endocarditis potential aneurysmal dilatation rupture.3-5 Here we presented a rare case CAF with multiple origins involving anterior descending (LAD), circumflex...

Based on visible light communication technology which has been a research hotspot in the field of com-munication, this paper reviews background communication, illustrates basic system architecture and explores progress around five frontier directions: material chips, high-speed systems, multiplexing networks, underwater machine learning. The challenges faced by are analyzed. Finally, looking forward to prospect communication: intelligent era future, will become an indispensable part networks...

Objectives: To assess the associations of mortality risk with fruit, vegetable, and legume intake in a prospective urban rural epidemiology study China (PURE-China). Methods: 43,676 individuals aged 35–70 years free cardiovascular diseases at recruitment were followed up prospectively total observation time 313,682 person-years. Diet was assessed using validated food frequency questionnaire. The main clinical outcomes mortality, non-cardiovascular mortality. Cox frailty models center as...

Seasonal Influenza H3N2 virus poses a great threat to public health, but its vaccine efficacy remains suboptimal. One critical step in influenza production is the viral passage embryonated eggs. Recently, strength of egg adaptation was found be rapidly increasing with time driven by convergent evolution at set functionally important codons hemagglutinin (HA1). In this study, we aim take advantage negative correlation between and effectiveness (VE) develop computational tool for selecting...

Seasonal H3N2 influenza evolves rapidly, leading to an extremely poor vaccine efficacy. Substitutions employed during production using embryonated eggs (i.e., egg passage adaptation) contribute the efficacy (VE), but evolutionary mechanism remains elusive. Using unprecedented number of hemagglutinin sequences (n = 89,853), we found that fitness landscape adaptation is dominated by pervasive epistasis between two residues (186 and 194) multiple other positions. Convergent paths driven strong...