A5067848684- Nutrition and Health in Aging
- GDF15 and Related Biomarkers
- Cardiac electrophysiology and arrhythmias
- Receptor Mechanisms and Signaling
- Signaling Pathways in Disease
- Frailty in Older Adults
- Phosphodiesterase function and regulation
- Cardiac Fibrosis and Remodeling
- Ion channel regulation and function
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Apelin-related biomedical research
- Viral Infections and Immunology Research
- Tryptophan and brain disorders
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- HIV Research and Treatment
- Gut microbiota and health
- Cardiac Ischemia and Reperfusion
- Lipid metabolism and disorders
- Cytomegalovirus and herpesvirus research
- COVID-19 and Mental Health
- Cancer, Hypoxia, and Metabolism
- Renin-Angiotensin System Studies
- Mitochondrial Function and Pathology
- Fatty Acid Research and Health
Université Toulouse III - Paul Sabatier
2011-2024
Institut des Maladies Métaboliques et Cardiovasculaires
2012-2024
Inserm
2012-2024
Université de Toulouse
2012-2023
Hôpital Rangueil
2021
European Commission
2019
Centre National de la Recherche Scientifique
2014-2017
Université de Montpellier
2017
Associated Research (United States)
2015
Université Paris-Sud
2009-2014
Cardiac hypertrophy is promoted by adrenergic overactivation and can progress to heart failure, a leading cause of mortality worldwide. Although cAMP among the most well-known signaling molecules produced beta-adrenergic receptor stimulation, its mechanism action in cardiac not fully understood. The identification Epac (exchange protein directly activated cAMP) proteins as novel sensors for has broken dogma surrounding kinase A. However, their role regulation mature remain be defined. Here,...
cAMP is a powerful second messenger whose known general effector protein kinase A (PKA). The identification of binding protein, Epac, raises the question its role in Ca 2+ signalling cardiac myocytes. In this study, we analysed effects Epac activation on handling by using confocal microscopy isolated adult rat cardiomyocytes. [Ca ] i transients were evoked electrical stimulation and sparks measured quiescent was selectively activated analogue 8‐(4‐chlorophenylthio)‐2′‐ O...
Although the second messenger cyclic AMP (cAMP) is physiologically beneficial in heart, it largely contributes to cardiac disease progression when dysregulated. Current evidence suggests that cAMP produced within mitochondria. However, mitochondrial signaling and its involvement pathophysiology are far from being understood.To investigate role of MitEpac1 (mitochondrial exchange protein directly activated by 1) ischemia/reperfusion injury.We show Epac1 (exchange genetic ablation (Epac1-/-)...
Abstract Background How inflammation relates to intrinsic capacity (IC), the composite of physical and mental capacities, remains undefined. Our study aimed investigate cross‐sectional longitudinal associations between plasma inflammation‐related biomarkers IC in older adults. Methods This secondary analysis Multidomain Alzheimer Preventive Trial (MAPT) included 1238 community‐dwelling individuals with assessments from 12 60 months. Plasma C‐reactive protein (CRP), interleukin‐6 (IL‐6),...
Patients with COVID-19 may develop abnormal inflammatory response, followed in some cases by severe disease and long-lasting syndromes. We show here that vitro exposure to SARS-CoV-2 activates the expression of human endogenous retrovirus (HERV) HERV-W proinflammatory envelope protein (ENV) peripheral blood mononuclear cells from a subset healthy donors, ACE2 receptor infection-independent manner. Plasma and/or sera 221 patients different cohorts, infected successive variants including...
The cAMP-binding protein Epac is a therapeutic target for the treatment of various diseases such as cardiac hypertrophy and tumor invasion. This points out importance to develop inhibitors better understand involvement these cAMP sensors in physiology pathophysiology. Here, we have developed functional fluorescence-based high-throughput assay with Z' value around 0.7 screening Epac-specific antagonists. We identified an Epac1 inhibitor compound named CE3F4 that blocked guanine nucleotide...
Objective The gut–brain axis is considered as a major regulatory checkpoint in the control of glucose homeostasis. detection nutrients and/or hormones duodenum informs hypothalamus host's nutritional state. This process may occur via hypothalamic neurons modulating central release nitric oxide (NO), which turn controls entry into tissues. enteric nervous system (ENS) modulates intestinal contractions response to various stimuli, but importance this interaction homeostasis brain unknown. We...
Stimulation of β-adrenergic receptors (β-AR) increases cAMP production and contributes to the pathogenesis cardiac hypertrophy failure through poorly understood mechanisms. We previously demonstrated that Exchange protein directly activated by 1 (Epac1)-induced in primary cardiomyocytes. Among mechanisms triggered stress, autophagy has been highlighted as a protective or harmful response. Here, we investigate whether Epac1 promotes how altered an impact on Epac1-induced cardiomyocyte...
Human endogenous retroviruses (HERVs) are remnants of infections that took place several million years ago and represent around 8% the human genome. Despite evidence implicating increased expression HERV type W envelope (HERV-W ENV) in schizophrenia bipolar disorder, it remains unknown whether such is associated with distinct clinical or biological characteristics symptoms. Accordingly, we performed unsupervised two-step clustering a multivariate data set included HERV-W ENV protein...
In the heart, cAMP is a key regulator of excitation–contraction coupling and its biological effects are mainly associated with activity protein kinase A (PKA). The aim this study was to investigate contribution cAMP-binding Epac (Exchange directly activated by cAMP) in regulation contractile properties rat ventricular cardiac myocytes. We report that both PKA increased sarcomere contraction but through opposite mechanisms. Differently from PKA, selective activation analog...
Decreasing duodenal contraction is now considered as a major focus for the treatment of type 2 diabetes. Therefore, identifying bioactive molecules able to target enteric nervous system, which controls motility intestinal smooth muscle cells, represents new therapeutic avenue. For this reason, we chose study impact oral galanin on system in diabetic mice.Enteric neurotransmission, contraction, glucose absorption, modification gut-brain axis, and metabolism (glucose tolerance, insulinemia,...
Background— Cardiac hypertrophy is an early hallmark during the clinical course of heart failure and regulated by various signaling pathways. However, molecular mechanisms that negatively regulate these signal transduction pathways remain poorly understood. Methods Results— Here, we characterized Carabin, a protein expressed in cardiomyocytes was downregulated cardiac human failure. Four weeks after transverse aortic constriction, Carabin-deficient (Carabin −/− ) mice developed exaggerated...
Post-infarction remodelling is accompanied and influenced by perturbations in mitogen-activated protein kinase (MAPK) signalling. The growth arrest DNA-damage-inducible 45 (Gadd45) proteins are small acidic involved DNA repair modulation of MAPK activity. Little known about the role Gadd45 heart. Here, we explored potential contribution gamma (γ) isoform to acute late phase heart failure (HF) after myocardial infarction (MI) determined mechanisms underlying Gadd45γ actions. up-regulated...
Objective The enteric nervous system (ENS) plays a key role in controlling the gut-brain axis under normal and pathological conditions, such as type 2 diabetes. discovery of intestinal actors, enterosynes, able to modulate ENS-induced duodenal contraction is considered an innovative approach. Among all factors, understanding gut microbes glycaemia still developed. We studied whether modulation microbiota by prebiotics could permit identification novel enterosynes. Design measured effects on...
Abstract Background Vitality is conceptually considered as the underlying capacity influencing other intrinsic (IC) domains and being related to nutrition, physiological reserve biological ageing. However, there no consensus on its operationalisation. Objective To investigate structure magnitude of association vitality with IC functional difficulties using three operational definitions vitality. Methods We included 1,389 older adults from Multidomain Alzheimer Preventive Trial data Mini...
Abstract Epidemiology has repeatedly associated certain infections with a risk of further developing psychiatric diseases. Such can activate retro-transposable genetic elements (HERV) known to trigger immune receptors and impair synaptic plasticity neuroreceptors. Since the HERV-W ENV protein was recently shown co-cluster pro-inflammatory cytokines in subgroup patients schizophrenia or bipolar disorder, we questioned influence COVID-19 pandemic on psychosis spectrum disorders (PSD). Present...
Cyclic adenosine monophosphate (cAMP) is a master regulator of mitochondrial metabolism but its precise mechanism action yet remains unclear. Here, we found that dietary saturated fatty acid (FA), palmitate increased intracellular cAMP synthesis through the palmitoylation soluble adenylyl cyclase in cardiomyocytes. further induced exchange protein directly activated by cyclic AMP 1 (Epac1) activation, which was upregulated myocardium obese patients. Epac1 enhanced activity key enzyme...
The artemisinin derivative, artemether, has antimalarial activity with potential neurotoxic and cardiotoxic effects. Artemether in nanocapsules (NC-ATM) is more efficient than free artemether for reducing parasitaemia increasing survival of Plasmodium berghei-infected mice. NCs also prevent prolongation the QT interval ECG. Here, we assessed cellular cardiotoxicity how this toxicity was prevented by nanoencapsulation. Mice were treated NC-ATM orally (120 mg·kg-1 twice daily) 4 days. Other...
Late-life aging is often associated with appetite reduction and weight loss. Physical activity (PA) may prevent these processes, but the molecular mechanisms involved remain elusive. The present study investigated putative mediating aspect of growth differentiation factor 15 (GDF-15), a stress signalling protein in aging, exercise control, on association between PA late-life-associated loss.One thousand eighty-three healthy adults (63.8% women) aged 70 years over who participated Multidomain...