A5100401417- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Biosensors and Analytical Detection
- COVID-19 Clinical Research Studies
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Viral Infections and Outbreaks Research
- COVID-19 diagnosis using AI
- Complex Network Analysis Techniques
- Pancreatitis Pathology and Treatment
- Long-Term Effects of COVID-19
- Mental Health Research Topics
- HIV/AIDS Research and Interventions
- Mosquito-borne diseases and control
- Infection Control and Ventilation
- Dermatological and COVID-19 studies
- Vector-borne infectious diseases
- COVID-19 epidemiological studies
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- Hepatitis B Virus Studies
- Trauma and Emergency Care Studies
- Parvovirus B19 Infection Studies
- COVID-19 and Mental Health
- Opinion Dynamics and Social Influence
Fudan University
2022-2024
Huashan Hospital
2022-2023
Suzhou Research Institute
2023
National Institute of Metrology
2020-2022
Chinese Academy of Agricultural Sciences
2022
Jilin Medical University
2022
Jilin University
2022
Anhui University
2022
Tianjin Medical University
2021
Academy of Military Medical Sciences
2021
BACKGROUND The outbreak of COVID-19 caused by a novel Coronavirus (termed SARS-CoV-2) has spread to over 140 countries around the world. Currently, reverse transcription quantitative qPCR (RT-qPCR) is used as gold standard for diagnostics SARS-CoV-2. However, positive rate RT-qPCR assay pharyngeal swab samples are reported vary from 30∼60%. More accurate and sensitive methods urgently needed support quality assurance or an alternative diagnostic approach. METHODS We established digital PCR...
Abstract Ebola virus (EBOV), one of the deadliest viruses, is cause fatal disease (EVD). The underlying mechanism viral replication and EBOV-related hemorrhage not fully understood. Here, we show that EBOV VP35, a cofactor RNA-dependent RNA polymerase, binds human A kinase interacting protein (AKIP1), which consequently activates (PKA) PKA-downstream transcription factor CREB1. During infection, CREB1 recruited into ribonucleoprotein complexes in inclusion bodies (VIBs) employed for...
Abstract Tick‐borne encephalitis virus (TBEV) is an important causative agent that causes neurological infections in humans and animals. In recent years, only few epidemiological surveys on TBEV have been conducted China. The purpose of this study was to determine the prevalence subtype ticks northeastern (NE) A total 3799 questing were collected NE China between April 2015 June 2016. Ticks pooled tested for RNA using semi‐nested reverse transcriptase‐polymerase chain reaction. Positive...
The pandemic of the novel coronavirus disease 2019 (COVID-19) has caused severe harm to health people all around world. Molecular detection pathogen, acute respiratory syndrome 2 (SARS-CoV-2), played a crucial role in control disease. Reverse transcription digital PCR (RT-dPCR) been developed and used SARS-CoV-2 RNA as an absolute quantification method. Here, interlaboratory assessment was organized by National Institute Metrology, China (NIMC), using vitro transcribed samples, among ten...
The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to unprecedented social and economic disruption. Many nucleic acid testing (NAT) laboratories in China have been established control the epidemic better. This proficiency (PT) aims evaluate participants' performance qualitative quantitative SARS-CoV-2 NAT explore factors that contribute differences detection capabilities. Two different concentrations of RNA samples (A, B) were used for PT. Pseudovirus...
<title>Abstract</title> <bold>Objectives:</bold> The COVID-19 pandemic poses ongoing challenges to global public health systems, emphasizing the critical necessity for efficient diagnostic and prognostic markers. This study evaluates MAGLUMI® SARS-CoV-2 Ag N protein chemiluminescent immunoassay (MAG-CLIA) its analytical performance role in predicting disease severity prognosis among severe patients with comorbidities. <bold>Methods:</bold> Analytical validation of plasma MAG-CLIA encompassed...
The clinical spectrum of COVID-19 pneumonia is varied. Thus, it important to identify risk factors at an early stage for predicting deterioration that require transferring the patients ICU. A retrospective multicenter study was conducted on admitted designated hospitals in China from Jan 17, 2020, Feb 2020. Clinical presentation, laboratory data, and quantitative CT parameters were also collected. result showed increasing risks ICU admission associated with age &gt; 60 years (odds ratio...
Abstract Background This retrospective study aimed to characterize the distribution of HIV-1 genotypes and prevalence drug resistance mutations in people with antiretroviral treatment (ART) failure Suzhou City, China. Methods Pol gene viruses blood samples EDTA anticoagulants from 398 patients failed antiviral was successfully amplified by using an in-house assay. Drug were analyzed Stanford HIV Resistance Database system ( https://hivdb.stanford.edu/hivdb/by-mutations/ ). determined REGA...
The Kirkpatrick Model has been widely used in nursing staff training. This article reviews the origin and content of as well its application training order to provide a reference for establishing mechanism improving professional quality staff. Key words: Review; Nursing care; Kirkpatrick Model; Training
Abstract Background: The ongoing Coronavirus disease 2019 (COVID-19) pandemic has spread across the globe and is representing a huge challenge for all human population. Many commercial qRT-PCR assays have been developed to detect SARS-CoV-2, but related method validation especially sensitivity evaluation insufficient, resulting in some false-negative cases reported. Methods: analytical of nine brands kits detecting SARS-CoV-2 was evaluated parallel based on newly certified reference...
Abstract Background: Lymphatic choriomeningitis virus (LCMV), belongs to the viral family Arenaviridae , genus Mammarenavirus can cause aseptic meningitis in humans. Rodents are usually considered as natural reservoir host of LCMV. But recent study indicates that LCMV may be transmitted by ticks northeastern (NE) China. To further explore epidemic distribution LCMV, we conducted prevalence and genetic characterization Jilin Province, NE Methods: Ticks were collected Huadian, Dunhua, Jiaohe,...
Abstract Background This study evaluated the quantitative detection of SARS-CoV-2 antigen by MAGLUMI chemiluminescent immunoassay (MAG-CLIA) in COVID-19 patients during peak Shanghai epidemics a tertiary hospital Shanghai. Methods Analytical performances MAG-CLIA were evaluated, including precision, limit quantitation (LoQ), linearity and specificity. Nasopharyngeal specimens from 232 who RT-qPCR positive 477 healthy donors included to evaluate diagnostic performance. The performance Wondfo...
To implement epidemic prevention among entry personnel, Shanghai has launched a number of Medical Observation Sites (Observation Site) for which high expectations are set. Ours is one such Site.As part novel project, we did not have any previous experience to use as reference. Despite some challenges, achieved satisfactory outcomes by establishing stepwise approach inbound passenger management, including the division working area Site, dynamic management rooms, closed-loop isolated...