A5025237905- Metabolomics and Mass Spectrometry Studies
- Liver Disease Diagnosis and Treatment
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Lipid metabolism and biosynthesis
- Metabolism and Genetic Disorders
- Cardiovascular Function and Risk Factors
- Peroxisome Proliferator-Activated Receptors
- Diet and metabolism studies
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Metabolism, Diabetes, and Cancer
- Tryptophan and brain disorders
- Genetic Associations and Epidemiology
- Cholesterol and Lipid Metabolism
- Lipoproteins and Cardiovascular Health
- RNA modifications and cancer
- Immune cells in cancer
- Adipokines, Inflammation, and Metabolic Diseases
- Lipid metabolism and disorders
- Retinal Diseases and Treatments
- Diet, Metabolism, and Disease
- Alcohol Consumption and Health Effects
- Chronic Obstructive Pulmonary Disease (COPD) Research
Shandong Provincial Hospital
2022-2025
Shandong First Medical University
2022-2025
Shandong University
2022-2025
Shanghai Clinical Research Center
2023-2025
Shandong Center for Disease Control and Prevention
2023-2025
Shandong Academy of Chinese Medicine
2023-2025
Dalian Institute of Chemical Physics
2017-2022
Chinese Academy of Sciences
2017-2022
University of Chinese Academy of Sciences
2018-2022
Lipid coverage is crucial in comprehensive lipidomics studies challenged by high diversity lipid structures and wide dynamic range levels. Current state-of-the-art technologies are mostly based on mass spectrometry (MS), including direct-infusion MS, chromatography-MS, matrix-assisted laser desorption ionization (MALDI) imaging each with its pros cons. Due to the need or favorability for measurement of isomers isobars, chromatography-MS preferable profiling. The ultra-high performance liquid...
Diabetic retinopathy (DR) is the main cause of vision loss or blindness in working age adults worldwide. The lack effective diagnostic biomarkers for DR leads to unsatisfactory curative treatments. To define potential metabolite diagnosis, a multiplatform-based metabolomics study performed. In this study, total 905 subjects with diabetes without (NDR) and at different clinical stages are recruited. Multiplatform methods used characterize serum metabolic profiles screen validate biomarkers....
Rationale: Increased lipid droplet (LD) formation has been linked to tumor metastasis, stemness, and chemoresistance in various types of cancer. Here, we revealed that LD is critical for the adaptation sorafenib hepatocellular carcinoma (HCC) cells. We aim investigate function its regulatory mechanisms HCC. Methods: The key proteins responsible were screened by both metabolomics proteomics sorafenib-resistant HCC cells further validated immunoblotting immunofluorescence staining. Biological...
Abstract Under normal conditions, insulin promotes hepatic de novo lipogenesis (DNL). However, during resistance (IR), when signalling is blunted and accompanied by hyperinsulinaemia, the promotion of DNL continues unabated steatosis increases. Here, we show that WD40 repeat-containing protein 6 (WDR6) IR. Mechanistically, WDR6 interacts with beta-type catalytic subunit serine/threonine-protein phosphatase 1 (PPP1CB) to facilitate PPP1CB dephosphorylation at Thr316, which subsequently...
Carnitines play important roles in fatty acid oxidation and branched chain amino metabolism. The disturbance of acylcarnitines is associated with occurrence development many diseases. Comprehensive acylcarnitine identification can greatly benefit their targeted detection, following disease differential diagnosis possible mechanism study. In this study, we developed a novel strategy to identify as based on liquid chromatography–high-resolution mass spectrometry (LC–HRMS). layer–layer...
Metabolite and lipid profilings usually need two liquid chromatography-mass spectrometry (LC-MS) methods because of a great polarity difference. A pseudotargeted metabolomics method as novel emerging approach can integrate the advantages nontargeted targeted methods. Here, we aim to establish comprehensive for metabolome lipidome by using parallel column-based two-dimensional LC (PC-2DLC)-MS approach. To simultaneously extract many polar metabolites nonpolar lipids possible, systematically...
<title>Abstract</title> <bold>Background</bold> Serum ether-linked phospholipids (ePLs) have gained attention in metabolic disease research. Postmenopausal women face a higher risk of developing atherosclerosis (AS), yet current methods for prediction and understanding AS pathophysiology remain limited. This study aimed to identify ePL biomarkers linked AS, assess their chemical composition relation risk, explore whether impact is mediated by clinical factors postmenopausal women....
Abstract Background The causal relationship between basal metabolic rate (BMR) and infectious diseases remains elusive. This study aims to clarify this association. Methods analyzed genome-wide association studies (GWASs) data from the UK Biobank FinnGen cohorts investigate BMR in European populations. Mendelian randomization (MR) analysis was initially employed, followed by multivariable (MVMR) account for potential confounders. Mediation further confirmed significant relationships....
The pseudotargeted metabolomics method integrates advantages of nontargeted and targeted analysis because it can acquire data metabolites in the multireaction monitoring (MRM) mode mass spectrometry (MS) without needing standards. key is ion-pair information collection from samples to be analyzed. It well-known that sequential windowed acquisition all theoretical Fragment ion (SWATH) MS MS2 a maximum extent. To expediently as many ion-pairs possible with optimal collision energy (CE), an...
Metabolic syndrome (MTS) is a cluster of concurrent metabolic abnormal conditions. MTS and its component diseases are heterogeneous closely related, making their relationships complicated, thus hindering precision treatment. Methods: We collected seven groups samples (group a: healthy individuals; group b: obesity; c: MTS; d: hyperglycemia, e: hypertension, f: hyperlipidemia; g: type II diabetes, n=7 for each group). examined the molecular characteristics sample by metabolomic, proteomic...
Gliomas are the most aggressive phenotypes of brain tumors and classified into four grades according to malignancy degree by World Health Organization. Metabolic profiling can provide an overview metabolic reprogramming at a specific stage tumor initiation development. Studies about alterations related different gliomas helpful understand molecular mechanism for progression glioma. In current study, metabolomics lipidomics analyses based on chromatography-mass spectrometry were performed...
Objective Dyslipidemia is a hallmark of diabetes mellitus (DM). However, specific lipid molecules closely associated with the initiation and progression remain unclear. We used pseudotargeted lipidomics approach to evaluate complex changes that occurred long before diagnosis type 2 (T2DM) identify novel markers for screening prediabetes (PreDM) T2DM in patients from multiple communities. Methods Four hundred eighty-one subjects consisting T2DM, three subtypes PreDM, normal controls (NC) were...
Abstract In a Chinese prospective cohort, 500 patients with new‐onset type 2 diabetes (T2D) within 4.61 years and matched healthy participants are selected as case control groups, randomized into discovery validation sets to discover the metabolite changes before T2D onset related diabetogenic loci. A serum metabolomics analysis reveals that 81 metabolites changed significantly onset. Based on binary logistic regression, eight defined biomarker panel for prediction. Pipecolinic acid,...
Uremia has been a rapidly increasing health problem in China. Hemodialysis (HD) is the main renal replacement therapy for uremia. The results of large-scale clinical trials have shown that HD pattern crucial long-term prognosis maintenance hemodialysis (MHD) uremic patients. Plasma metabolism very important revealing biological insights linked to therapeutic effects on Alteration plasma metabolites patients response reported. However, HD-pattern-dependent changes remain poorly understood. To...
In recent years, the impact of lipotoxicity on male fertility has received extensive attention, especially Sertoli cells (SCs). SCs, energy metabolism is important as disorders result in infertility eventually. However, underlying mechanism SCs remains unknown. Advances high-throughput metabolomics and lipidomics measurement platforms provide powerful tools to gain insights into complex biological systems. Here, we aimed explore potential molecular mechanisms palmitic acid (PA) regulating...
The pituitary gland is a small but important organ located in the base of brain. Although mostly noncancerous, adenomas (PAs) can cause serious health problems such as headaches, visual field defects, double vision, and hypopituitarism by invasion regional structures. Nonfunctioning PAs (NFPAs) approximately account for one-third manifested no circulating hormone hypersecretion. Lipid reprogramming has been recognized hallmark tumor cells proven to play crucial role tumorigenesis. However,...
Background: There is an inconsistent association between overweight/obesity and chronic obstructive pulmonary disease (COPD). Considering that different metabolic characteristics exist among individuals in the same body mass index (BMI) category, classification of based on status may facilitate risk assessment COPD. Our study aimed to explore relationship phenotypes unplanned readmission patients with Methods: We conducted a retrospective cohort using Nationwide Readmissions Database (NRD)....