A5032129414- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Cancer Immunotherapy and Biomarkers
- RNA Research and Splicing
- CAR-T cell therapy research
- Plant Molecular Biology Research
- Immunotherapy and Immune Responses
- Telomeres, Telomerase, and Senescence
- Ferroptosis and cancer prognosis
- Mesenchymal stem cell research
- RNA Interference and Gene Delivery
- Protein Degradation and Inhibitors
- Neurobiology and Insect Physiology Research
- CRISPR and Genetic Engineering
- Pancreatic and Hepatic Oncology Research
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Testicular diseases and treatments
- Melanoma and MAPK Pathways
- vaccines and immunoinformatics approaches
- Histone Deacetylase Inhibitors Research
- Toxin Mechanisms and Immunotoxins
- Tissue Engineering and Regenerative Medicine
- Circular RNAs in diseases
University of Szeged
2014-2025
HUN-REN Szegedi Biológiai Kutatóközpont
2024-2025
Recent studies have suggested that a sub-complex of RNA polymerase II composed Rpb4 and Rpb7 couples the nuclear cytoplasmic stages gene expression by associating with newly made mRNAs in nucleus, contributing to their translation degradation cytoplasm. Here we show yeast two hybrid co-immunoprecipitation experiments, followed ribosome fractionation fluorescent microscopy, subunit Ccr4-Not complex, Not5, is essential nucleus for functions Rpb4. Not5 interacts Rpb4; it required presence...
Drug resistance is a significant challenge in pancreatic ductal adenocarcinoma (PDAC), where stromal elements such as adipose-derived mesenchymal stem cells (ASCs) contribute to chemoresistant tumor microenvironment (TME). This study explored the effects of oxaliplatin (OXP) and 5-fluorouracil (5-FU) on PDAC (Capan-1) ASCs investigate mechanisms chemoresistance. While OXP 5-FU reduced Capan-1 viability dose- time-dependent manner, demonstrated high resistance, maintaining > 90% even at...
Introduction: Adipose tissue-derived mesenchymal stem cells are promising contributors to regenerative medicine, exhibiting the ability regenerate tissues and modulate immune system, which is particularly beneficial for addressing chronic inflammatory ulcers wounds. Despite their inherent capabilities, research suggests that pretreatment amplifies therapeutic effectiveness. Methods: Our experimental design exposed adipose-derived six factors 24 h. We subsequently evaluated gene expression...
Breast cancer is the most frequent with a high fatality rate amongst women worldwide. Diagnosing at an early stage challenging, and due to limitations of currently used techniques, including mammography imaging diagnostics, it still remains unascertained. Serum biomarkers can be solution for this as they isolated in less painful, more cost-effective, minimally invasive manner. In study, we shed light on relevant role multiple microRNAs (miRNAs) potential breast diagnosis. We monitored...
The utilization of PD1 and CTLA4 inhibitors has revolutionized the treatment malignant melanoma (MM). However, resistance to targeted immune-checkpoint-based therapies still poses a significant problem.
In eukaryotes the TFIID complex is required for preinitiation assembly which positions RNA polymerase II around transcription start sites. On other hand, histone acetyltransferase complexes including SAGA and ATAC, modulate at several steps through modification of specific core residues. this study we investigated function Drosophila melanogaster proteins TAF10 TAF10b, are subunits dTFIID dSAGA, respectively. We generated a mutation eliminated production both orthologues. The simultaneous...
Ubiquitylation is critical for preventing aberrant DNA repair and efficient maintenance of genome stability. As deubiquitylases (DUBs) counteract ubiquitylation, they must have a great influence on many biological processes, including damage response. To elucidate the role DUBs in Drosophila melanogaster, systematic siRNA screening was applied to identify with reduced survival rate following exposure ultraviolet X-ray radiations. secondary validation, we direct repeat (DR)-white reporter...
DNA double-strand breaks are one of the most deleterious lesions for cells, therefore understanding macromolecular interactions repair-related mechanisms is essential. damage triggers transcription silencing at site, leading to removal elongating RNA polymerase II (S2P RNAPII) from this locus, which provides accessibility repair factors lesion. We previously demonstrated that following block, p53 plays a pivotal role in elongation by interacting with S2P RNAPII. In current study, we reveal...
UV-induced DNA damage response and repair are extensively studied processes, as any malfunction in these pathways contributes to the activation of tumorigenesis. Although several proteins involved cellular mechanisms have been described, entire cascade has remained unexplored. To identify new players repair, we performed a microarray screen, which found SerpinB10 (SPB10, Bomapin) one most dramatically upregulated genes following UV irradiation. Here, demonstrated that an increased mRNA level...
Abstract The utilization of PD1 and CTLA4 inhibitors has revolutionized the treatment malignant melanoma (MM). However, resistance to targeted immune-checkpoint-based therapies still poses a significant problem. Here we mine large scale MM proteogenomic data integrating it with cell line dependency screen, drug sensitivity identify druggable targets forecast efficacy resistance. Leveraging protein profiles from established subtypes molecular structures 82 cancer drugs, identified nine...
Testicular cancer is the most common solid malignancy among men aged 15-35. Radical orchiectomy and platinum-based chemotherapy (BEP) are curative in majority of patients, including advanced, metastatic cases. According to current urooncology guidelines all non-seminoma patients harbouring post-chemotherapy residual masses ≥ 1 cm should undergo salvage retroperitoneal lymph node dissection (RPLND). However, only 10% tumors contain viable disease. To assess patient outcomes complications...
Abstract While Immune checkpoint inhibition (ICI) therapy shows significant efficacy in metastatic melanoma, only about 50% respond, lacking reliable predictive methods. We introduce a panel of six proteins aimed at predicting response to ICI therapy. Evaluating previously reported two untreated melanoma cohorts, we used published model (EaSIeR score) identify potential distinguishing responders and non-responders. Six initially identified the cohort correlated with predicted cohort....
Introduction While Immune checkpoint inhibition (ICI) therapy shows significant efficacy in metastatic melanoma, only about 50% respond, lacking reliable predictive methods. We introduce a panel of six proteins aimed at predicting response to ICI therapy. Methods Evaluating previously reported two untreated melanoma cohorts, we used published model (EaSIeR score) identify potential distinguishing responders and non-responders. Results Six initially identified the cohort correlated with...
Cells have evolved various DNA repair mechanisms to prevent damage from building up. Malfunctions during can influence cellular homeostasis because they bring on genomic instability through the improper recognition of or dysregulation process. Maintaining proper is also essential for stem cells (SCs), as provide a differentiated cell population living organism. SCs are regularly used in personalized therapy. Patients must be treated with specific activators produce these effectively. This...
DNA repair pathways trigger robust downstream responses, making it challenging to select suitable reference genes for comparative studies. In this study, our goal was identify the most housekeeping perform comparable molecular analyses damage-related Choosing applicable is important in any kind of target gene expression-related quantitative since using improperly may result false data interpretation and inaccurate conclusions. We evaluated expressional changes eight well-known (i.e., 18S...
Transcription of eukaryotic genes requires the cooperative action RNA polymerase complex, general transcription factors (TFIIB, TFIID, TFIIE, TFIIF and TFIIH) chromatin modifiers. The TFIID complex contributes to transcriptional activation by several mechanisms has a subunit with associated histone acetyltransferase (HAT) activity. modifier SAGA both HAT deubiquitylase (DUB) activities. share TBP-associated (TAFs), but not their subunit. Recently, duplicated TAF proteins have been identified...
Abstract DNA double-strand breaks are the most deleterious lesions for cells, therefore understanding macromolecular interactions in repair-related mechanisms is essential. damage triggers transcription silencing at site, leading to removal of elongating RNA polymerase II (S2P RNAPII) from this locus, which provides accessibility repair factors lesion. Ataxia-telangiectasia mutated (ATM) and DNA-dependent protein kinase (DNAPK) two main regulatory kinases homologous recombination...