A5068183454- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Immune cells in cancer
- Orthopedic Infections and Treatments
- Surgical site infection prevention
- Sepsis Diagnosis and Treatment
- Bone fractures and treatments
- Appendicitis Diagnosis and Management
- Intestinal and Peritoneal Adhesions
- Intraperitoneal and Appendiceal Malignancies
- Hip and Femur Fractures
- Neonatal Respiratory Health Research
- Lung Cancer Research Studies
- Ultrasound in Clinical Applications
- Lung Cancer Diagnosis and Treatment
- Orthopedic Surgery and Rehabilitation
- Orthopaedic implants and arthroplasty
- S100 Proteins and Annexins
- Blood disorders and treatments
- Cardiac, Anesthesia and Surgical Outcomes
- Lung Cancer Treatments and Mutations
- Antimicrobial Resistance in Staphylococcus
- Intensive Care Unit Cognitive Disorders
Netherlands Comprehensive Cancer Organisation
2024
Collaborative Group (United States)
2024
University of Amsterdam
2023
University Medical Center Utrecht
2017-2020
Wilhelmina Children's Hospital
2020
Heidelberg University
2019
University Hospital Heidelberg
2019
Fracture-related infection (FRI) is an important complication following surgical fracture management. Key to successful treatment accurate diagnosis. To this end, microbiological identification remains the gold standard. Although a structured approach towards sampling specimens for microbiology seems logical, there no consensus on culture protocol FRI. The aim of study evaluate effect fracture-related infections compared ad-hoc sampling.We conducted pre-/post-implementation cohort that...
Phagocytosis by neutrophils is a key process in the innate immune response against invading microorganisms. Despite reported heterogeneity other functions, little known regarding differences phagocytosis individual cells. Therefore, we tested hypothesis that present neutrophil compartment its potency to phagocytize bacteria.Phagocytosis assays were performed suspension with isolated and Staphylococcus aureus expressing different fluorescent proteins at MOIs between 1 10. Repetitive addition...
Phagocytes such as granulocytes and monocytes are fundamental players in the innate immune system. Activation of these cells can be quantified by measurement activation marker expression using flow cytometry. Analysis receptor on inflammatory facilitates diagnosis diseases used to determine extent inflammation. However, several major limitations this analysis precludes application inflammation monitoring clinical practice. Fast automated would minimalize ex vivo manipulation allow...
Infection after osteosynthesis is an important complication with significant morbidity and even mortality. These infections are often caused by biofilm-producing bacteria. Treatment algorithms dictate aggressive approach surgical debridement antibiotic treatment. The aim of this study to analyze the effect such standardized treatment regime implant retention for acute, existing <3 weeks, infection osteosynthesis.We conducted a retrospective 2-year cohort in single, level 1 trauma center on...
Organ dysfunction remains a major cause of morbidity after trauma. The development organ is determined by the inflammatory response, in which neutrophils are important effector cells. A femoral fracture particularly predisposes for dysfunction. This study investigated chronologic relation between neutrophil characteristics and trauma patients with fracture.Patients presenting at University Medical Center Utrecht 2007 2013 were included. Data from standard hematological analyzers recorded on...
Infection near metal implants is a problem that presents challenging treatment dilemmas for physicians. The aim of this study was to analyse the efficacy two protocols acute fracture-related infections.Seventy-one patients in level-1 trauma centres Netherlands were retrospectively included study. These had different standardised infection after osteosynthesis: 39 selected from protocol A and 32 B. Both involve immediate surgical debridement soft tissue coverage, but differ antibiotic...
Leukocyte viability (determined by e.g. propidium iodide [PI] staining) is automatically measured hematology analyzers to check for delayed bench time. Incidental findings in fresh blood samples revealed the existence of leukocytes with decreased critically ill surgical patients. Not much known about these cells and their functional and/or clinical implications. Therefore, we investigated incidence leukocyte viability, implications functioning its relation outcomes. An automated alarm was...
After major trauma neutrophil function is negatively affected (eg, diminished responsiveness to stimuli, chemotaxis, phagocytosis1), contributing increased susceptibility of infection. The initial presence banded, segmented, and hypersegmented neutrophils (based on CD16/CD62L expression/associated with nuclear segmentation) linked late infectious complications2 the development multi-organ failure3 during admission in patients. Therefore, it great importance recognize subsets obtain all...
To determine the accuracy of final judgements doctors at emergency department (ED) and radiologists to differentiate between complicated uncomplicated acute appendicitis, because these have different treatment options.
Introduction: Distal forearm fractures are the most common cause for morbidity in otherwise healthy children. There is a wide variety of pediatric fractures. When sufficient force applied, complete ‘adult-type’ fracture may occur. By nature, these very unstable because disruption both cortices. Maintaining reduction key successful treatment. However, there no consensus on how to maintain completely displaced distal We evaluate success rate closed and cast immobilization. Methods: Subjects...