A5053843245- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- RNA modifications and cancer
- Lung Cancer Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Medical Imaging and Pathology Studies
- Radiomics and Machine Learning in Medical Imaging
- Aortic Disease and Treatment Approaches
- Lung Cancer Treatments and Mutations
- Pulmonary Hypertension Research and Treatments
- Cancer-related gene regulation
- Biopolymer Synthesis and Applications
- Cardiac Valve Diseases and Treatments
- Chemical Synthesis and Analysis
- Polyamine Metabolism and Applications
- Congenital Heart Disease Studies
- Pancreatic function and diabetes
- PI3K/AKT/mTOR signaling in cancer
- Medicinal Plant Pharmacodynamics Research
- Respiratory Support and Mechanisms
- Congenital heart defects research
- Cardiac Fibrosis and Remodeling
- Ferroptosis and cancer prognosis
Ningbo University
2013-2023
Ningbo Medical Center Lihuili Hospital
2013-2023
Zhejiang University
2010-2017
First Affiliated Hospital Zhejiang University
2013
Huaihua University
2011
Xian Yang Central Hospital
2011
Indiana University Bloomington
2008
Transforming growth factor-β (TGF-β) family members, including TGF-βs, activins, and bone morphogenetic proteins, exert diverse biological activities in cell proliferation, differentiation, apoptosis, embryonic development, many other processes. These effects are largely mediated by Smad proteins. Smad7 is a negative regulator for the signaling of TGF-β members. Dysregulation associated with pathogenesis variety human diseases. However, vivo physiological roles have not been elucidated due...
PLA2G7 gene product is a secreted enzyme whose activity associated with coronary heart disease (CHD). The goal of our study to investigate the contribution promoter DNA methylation risk CHD. Using bisulphite pyrosequencing technology, was measured among 36 CHD cases and well-matched controls. Our results indicated that there significant association between (adjusted P = 0.025). Significant gender-specific correlation observed age (males: adjusted r −0.365, 0.037; females: 0.373, 0.035). A...
Epithelial-mesenchymal transition (EMT) has been believed to be related with chemotherapy resistance in non-small cell lung cancer (NSCLC). Recent studies have suggested eIF5A-2 may function as a proliferation-related oncogene tumorigenic processes.We used viability assays, western blotting, immunofluorescence, transwell-matrigel invasion assay, wound-healing assay combined GC7 (a novel inhibitor) treatment or siRNA interference investigate the role of playing NSCLC chemotherapy.We found low...
This study compared microRNA (miRNA) expression profiles between rheumatic heart disease (RHD) patients and healthy controls to investigate their differential help elucidate mechanisms of action. Microarray analysis was used measure miRNA expression, a total 133 miRNAs were shown be significantly upregulated in RHD with controls, including miR-1183 miR-1299. A 137 miRNAs, miR-4423-3p miR-218-1-3p, downregulated patients. Quantitative real-time-PCR confirmed microarray findings for miR-1299...
Glucokinase encoded by GCK is a key enzyme that facilitates phosphorylation of glucose to glucose-6-phosphate. Variants gene were shown be associated with type 2 diabetes (T2D) and coronary heart disease (CHD). The goal this study was investigate the contribution gene-body methylation risk CHD.36 patients (18 males 18 females) 36 age- sex-matched controls collected for current research. DNA level CpG island (CGI) region on measured through sodium bisulfite conversion pyrosequencing...
Objective To study the effects of saikosaponin D (SSD) on proliferation and apoptosis in human non-small cell lung cancer lines, to explore underlying mechanisms. Methods Following treatment with D, A549 H1299 cells were assessed for anti-proliferation using cycle kit-8 assays, changes nuclear morphology 4′,6-diamidino-2-phenylindole (DAPI) staining, annexin V/propidium iodide double staining. Proliferation- apoptosis-related proteins detected by immunoblotting. Results Saikosaponin had...
We determined the role of microRNA (miR)-9 in regulating cisplatin chemoresistance nonsmall cell lung cancer (NSCLC) cells. miR-9 and eukaryotic translation initiation factor 5A2 (eIF5A2) levels were examined by reverse transcription–quantitative PCR. Cell Counting Kit-8 5-ethynyl-2′-deoxyuridine (EdU) assay used to determine effects mimic or inhibitor on NSCLC proliferation viability, respectively. Bioinformatics was analyze relationship between eIF5A2. Flow cytometry percentage apoptotic...
Patients with stage IV non-small cell lung cancer (NSCLC) comprise a heterogeneous group, and the optimal treatment for this group of patients is complex debatable. We aimed to assess effect local thoracic therapy combined chemotherapy on specific survival (CSS). To evaluate CSS four subgroups NSCLC according different modalities: modality Chemotherapy, Surgery, Radiation (Chem+Sur+RT), Chemotherapy (Chem+RT), Surgery (Chem+Sur), only (Chem Only) by analyzing Surveillance, Epidemiology, End...
In the past few years, immunotherapy of tumors has become an extensive research hotspot, and value IKZF family genes in tumor microenvironment also been increasingly recognized. However, expression IKAROS zinc finger 3 (IKZF3) gene human head neck squamous cell carcinoma (HNSCC) its prognostic were not reported for main subset until now. present study, we analyzed relationship between IKZF3 survival HNSCC patients. To evaluate potential as a biomarker comprehensively, multiple online...
The aim of the present study was to identify differentially methylated regions (DMRs) in patients with rheumatic heart disease and secondary pulmonary arterial hypertension (RHD-PAH). A genome-wide DNA methylation assay performed between 6 RHD-PAH healthy controls using an Illumina Infinium HumanMethylation450 BeadChip kit. Limma software package subsequently used significant DMRs. total 40 hypome-thylated 64 hypermethylated CpG sites were identified group control group. Gene Ontology Kyoto...
Valvular heart disease (VHD) is a common that affects blood flow. It usually requires surgery. complicated with pulmonary artery hypertension (VHD-PAH) may be lethal due to failure results from increased burden. important for these patients seek early treatment in order minimize the damage. However, there no reliable diagnosis method VHD. In this study, we found DNA methylation was at promoter of BMPR2 gene VHD compared healthy controls. This finding confirmed by an independent cohort study...
Esophageal squamous cell carcinoma (ESCC) is one of the most common clinical malignancies digestive system, characterized by high mortality but not evident early symptoms. Molecular markers for diagnostic and outcome prediction are urgently needed. Circular RNAs might play essential roles in progression ESCC.Hsa_circ_0000977 was identified using circRNA microarrays qRT-PCR. The value hsa_circ_0000977 calculated. We also examined vitro functions ECA109 TE12 ESCC cells to determine effect...
Rheumatic heart disease (RHD) is a serious autoimmune disease. The present study was aimed at identifying the differentially expressed proteins between patients with RHD and controls mitral valve prolapse. Nine nine prolapsed were enrolled for this study. Two-dimensional difference in-gel electrophoresis (2D-DIGE) matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) performed. A total of 39 protein spots differential expressions identified two groups (P...
Currently, the diagnosis and outcome of rheumatic valvular heart disease (RVHD) are less than ideal, there no accurate biomarkers. Circular RNA (circRNA) might participate in occurrence development RVHD.We use circRNA microarray to filter out target has_circ_0000437. qRT-PCR was used measure expression levels hsa_circ_0000437 RVHD plasma samples. We assessed diagnostic value RVHD. Cell function vitro experiment explore effect has_circ_0000437 on RVHD.Has_circ_0000437 is highly expressed (p <...
DNA methylation is known to regulate the expression of numerous genes but its role in pathogenesis thoracic aortic dissection (TAD) has remained largely elusive. In present study, methylome patients with TAD was analyzed using a microarray and bisulfite pyrosequencing used determine whether hypermethylation matrix metalloproteinase 2 (MMP2) specifically associated TAD. Chip-based whole-DNA analysis performed on 4 male healthy controls an Illumina HumanMethylation EPIC 850K BeadChip. The...
To determine whether up-regulation of miR-1183 targeting the gene for anti-apoptotic factor, B-cell lymphoma 2 (BCL-2) contributes to apoptosis in patients with rheumatic heart disease (RHD). Peripheral blood samples were isolated characterization. The function miRNA-1183 RHD using miRNA mimic on PBMCs and THP-1 cell models. binding Bcl-2 was confirmed by luciferase activity test. We also measured expression levels BCL-2 valve tissue from ELISA immunohistochemistry. In silico analysis...
Background Although most patients with tetralogy of Fallot undergo radical repair during infancy and childhood, that remain undiagnosed untreated until adulthood can still be treated. This study aimed to evaluate long-term outcomes adult who were treated surgically, determine the predictors postoperative pulmonary regurgitation. Methods Fifty-six underwent complete surgical repair. Forty-three (76.8%) required a transannular patch. Systolic, diastolic, mean pressure in main artery measured...