A5010689255- CAR-T cell therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Biosimilars and Bioanalytical Methods
- Virus-based gene therapy research
- Cancer, Hypoxia, and Metabolism
- Advancements in Semiconductor Devices and Circuit Design
- RNA modifications and cancer
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- High Altitude and Hypoxia
- HIV Research and Treatment
- Genetic factors in colorectal cancer
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Kruppel-like factors research
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Radiomics and Machine Learning in Medical Imaging
- Drug Transport and Resistance Mechanisms
- Frailty in Older Adults
- Epigenetics and DNA Methylation
- Health Systems, Economic Evaluations, Quality of Life
- Psoriasis: Treatment and Pathogenesis
- Immunotherapy and Immune Responses
- Diet and metabolism studies
- Cancer Research and Treatments
Union Hospital
2023-2025
University of Science and Technology of China
2024
Huazhong University of Science and Technology
2023-2024
State Key Laboratory of Information Engineering in Surveying Mapping and Remote Sensing
2023-2024
Army Medical University
2023-2024
Gansu University of Traditional Chinese Medicine
2024
Anhui Medical University
2024
Daping Hospital
2023
Beijing Sport University
2021-2023
Xinqiao Hospital
2023
Abstract Programmed cell death protein-1 (PD-1)-mediated immunosuppression has been proposed to contribute the limited clinical efficacy of chimeric antigen receptor T (CAR-T) cells in solid tumors. We generated PD-1 and (TCR) deficient mesothelin-specific CAR-T (MPTK-CAR-T) using CRISPR-Cas9 technology evaluated them a dose-escalation study. A total 15 patients received one or more infusions MPTK-CAR-T without prior lymphodepletion. No dose-limiting toxicity unexpected adverse events were...
Although chimeric antigen receptor T-cell (CAR-T) therapy development for B-cell malignancies has made significant progress in the last decade, broadening success to treating acute lymphoblastic leukemia (T-ALL) been limited. We conducted two clinical trials verify safety and efficacy of GC027, an "off-the-shelf" allogeneic CAR-T product targeting antigen, CD7. Here, we report 2 patients as case reports with relapsed/refractory T-ALL who were treated GC027.Both reported here open-label...
Abstract To improve clinical outcomes and shorten the vein-to-vein time of chimeric antigen receptor T (CAR-T) cells, we developed FasT CAR-T (F-CAR-T) next-day manufacturing platform. We report preclinical first-in-human studies evaluating safety, feasibility, preliminary efficacy CD19 F-CAR-T in B-cell acute lymphoblastic leukemia (B-ALL). cells demonstrated excellent proliferation with a younger cellular phenotype, less exhaustion, more effective tumor elimination compared to conventional...
Introduction There is no curative treatment available for patients with chemotherapy relapsed or refractory CD19+ B cells-derived acute lymphoblastic leukaemia (r/r B-ALL). Although CD19-targeting second-generation (2nd-G) chimeric antigen receptor (CAR)-modified T cells carrying CD28 4-1BB domains have demonstrated potency in advanced B-ALL, these 2 signalling endow CAR-T different and complementary functional properties. Preclinical results shown that third-generation (3rd-G) combining...
Background Regulating T-cell metabolism is crucial for their anticancer activity. Therefore, understanding the function and of human tumor-infiltrating T cells broad interest clinical importance. Methods CD3 + CD45 were sorted from adjacent area or tumor core hepatocellular carcinoma (HCC), then clusters heterogeneity further interrogated by single-cell transcriptomic profiling. 118 surgical samples patients with HCC histologically examined infiltration CD8 in tissue. Results Single-cell...
Purpose/background Microsatellite instability (MSI) status is a significant biomarker for the response to immune checkpoint inhibitors, 5-fluorouracil-based adjuvant chemotherapy, and prognosis in colorectal carcinoma (CRC). This study investigated predictive value of intratumoral-metabolic heterogeneity (IMH) conventional metabolic parameters derived from 18 F-FDG PET/CT MSI patients with stage I–III CRC. Methods was retrospective analysis 152 CRC pathologically proven who underwent...
Abstract Chimeric antigen receptor-engineered T (CAR-T) cells have shown promising efficacy in patients with relapsed/refractory B cell acute lymphoblastic leukemia (R/R B-ALL). However, challenges remain including long manufacturing processes that need to be overcome. We presented the CD19-targeting CAR-T product GC007F manufactured next-day (FasTCAR-T cells) and administered R/R B-ALL. A total of 21 over 14 years age CD19 + B-ALL were screened, enrolled infused a single infusion at three...
Abstract Cerebral malaria is the most serious complication of infection, with 26% surviving children having neurological sequelae, which may be caused by neuron damage, but mechanism not clear. Ferroptosis has been reported to play an important role in damage several nervous system diseases. However, occurrence ferroptosis experimental cerebral (ECM) pathogenesis still unknown. In this study, we firstly detected increased levels malondialdehyde (MDA) and iron, are indicators ferroptosis,...
Abstract T ‐helper ( h) cells, including h1, h2 and h17 may play an important role in the pathogenesis of psoriasis vulgaris PV ). Acitretin is effective treatment for ; however, its influence on h cells during unclear. This study aimed to investigate acitretin patients. patients n = 30) received p.o. (20 mg/day) 8 weeks. Sera skin biopsies were obtained before after treatment. Double‐labeled immunofluorescence was used analyze , lesions. Enzyme‐linked immunosorbent assay western blot...
Abstract Introduction: Patients with T cell malignancies usually have high relapse and mortality rates. Due to shared common surface antigen potential contamination by malignant cells, development of CAR-T therapies for r/r T-ALL has been lagged, regardless the costly lengthy process autologous production. To overcome these challenges, we developed a universal platform (TruUCAR™) that displayed superior expansion in patients without using preconditioning biologics such as αCD52 antibody....
BackgroundAlthough chimeric antigen receptor-modified T cells (CAR T) cell therapy has been widely reported in improving the outcomes of B-cell acute lymphoblastic leukemia (B-ALL), less research about feasibility and safety donor-derived CAR after allogeneic hematopoietic stem transplantation (allo-HSCT) was reported.MethodsThis phase 1 clinical trial aims to evaluate efficacy anti-CD19 (GC007g) B-ALL patients who relapsed allo-HSCT. This is registered with ClinicalTrials.gov,...
ObjectiveThe preoperative hemoglobin, albumin, lymphocyte, and platelet (HALP) score, a comprehensive marker of nutritional immunological status, has been found to be robust for tumor prognosis prediction. Here, we evaluated the use HALP in prognostic prediction tongue squamous cell carcinoma (TSCC).Study designPatients with TSCC were retrospectively recruited from years 2009–2019. Patient clinicopathological characteristics, along blood parameters, recorded on admission, cut-off value was...
A dendritic cell (DC)-based vaccine strategy could reduce the risk of recurrence and improve survival breast cancer patients. However, while therapy-induced apoptosis hepatocellular colorectal carcinoma cells can enhance maturation antigen presentation DCs, whether this effect occurs in is currently unknown. In present study, we investigated doxorubicin (ADM)-induced apoptotic MCF-7 on activation DCs. ADM-induced effectively induce immature DC (iDC) maturation. The mean fluorescence...
Exercise induces blood flow redistribution among tissues, leading to splanchnic hypoperfusion. Intestinal epithelial cells are positioned between the anaerobic lumen and highly metabolic lamina propria with an oxygen gradient. Hypoxia-inducible factor (HIF)-1α is pivotal in transcriptional response flux. In this study, pimonidazole hydrochloride staining was applied observe tissue hypoxia different organs, which might be affected by redistribution. The HIF-1α luciferase reporter ROSA26...
Abstract Rationale: Relapse is the main cause of death after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Unfortunately, there are no efficient methods to prevent relapse allo-HSCT. Chimeric antigen receptor T (CAR-T) cells have achieved favorable outcomes in treatment refractory/relapsed acute lymphoblastic leukemia (ALL) because their strong anti-leukemia activity. However, it unclear whether CAR-T constructed using viral systems can be used as preventive infusions...