A5075384045- Axon Guidance and Neuronal Signaling
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Wnt/β-catenin signaling in development and cancer
- RNA modifications and cancer
- Developmental Biology and Gene Regulation
- Nerve injury and regeneration
- CRISPR and Genetic Engineering
- Proteoglycans and glycosaminoglycans research
- Zebrafish Biomedical Research Applications
- Cancer-related gene regulation
- RNA Research and Splicing
- Connexins and lens biology
- Angiogenesis and VEGF in Cancer
- Hippo pathway signaling and YAP/TAZ
- Neurobiology and Insect Physiology Research
- Animal Genetics and Reproduction
- Polysaccharides and Plant Cell Walls
- Congenital heart defects research
- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Ubiquitin and proteasome pathways
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Epigenetics and DNA Methylation
- Neuroinflammation and Neurodegeneration Mechanisms
Kyushu University
1991-2024
Hirosaki University
2024
Research Institute for Production Development
2024
Kumamoto University
2012-2021
Japan Agency for Medical Research and Development
2015-2021
Nishi Niigata Chuo National Hospital
2017
Nagoya City University
2016
University of Debrecen
2012
The Geneva Association
2012
New York Proton Center
2012
Axon guidance proteins are critical for the correct wiring of nervous system during development. Several axon cues and their family members have been well characterized. More unidentified assumed to participate in formation extremely complex system. We identified a secreted protein, draxin, that shares no homology with known cues. Draxin inhibited or repelled neurite outgrowth from dorsal spinal cord cortical explants vitro. Ectopically expressed draxin growth caused misrouting chick...
Draxin, a recently identified axon guidance protein, is essential for the formation of forebrain commissures, and can mediate repulsion netrin-stimulated spinal commissural axons. Here, we report that draxin binds multiple netrin receptors: DCC (deleted in colorectal cancer), Neogenin, UNC5s (H1, H2, H3), DSCAM (Down's syndrome cell adhesion molecule). Since Dcc knockouts showed similar phenotype commissures formation, show here functional importance draxin/DCC interaction. Draxin interacts...
The Wnt signaling pathway is essential for the development of diverse tissues during embryogenesis. Signal transduction activated by binding proteins to type I receptor low-density lipoprotein receptor–related protein 5/6 and seven-pass transmembrane Frizzled (Fzd), which contains a Wnt-binding site in form cysteine-rich domain. Known extracellular antagonists can be subdivided into two broad classes depending on whether they bind primarily or 5/6. We show that secreted Tsukushi (TSK)...
The human gastrointestinal tract is colonized by a vast community of symbionts and commensals. Lactic acid bacteria (LAB) form group related, low-GC-content, gram-positive that are considered to offer number probiotic benefits general health. While the role LAB in microecology has been subject extensive study, little known about how commensal prokaryotic organisms directly influence eukaryotic cells. Here, we demonstrate generation multipotential cells from adult dermal fibroblast...
Abstract The thalamocortical tract carries sensory information to the neocortex. It has long been recognized that neocortical pioneer axons of subplate neurons are essential for development. Herein we report an axon guidance cue, draxin, is expressed in early-born neurons, including and necessary In draxin −/− mice, do not enter This phenotype sufficiently rescued by transgenic expression neurons. Genetic interaction data suggest acts through Deleted colorectal cancer (DCC) Neogenin (Neo1),...
Immunohistochemistry by using monoclonal antibodies named A5 and B2, which specifically recognize cell surface proteins the neuropilin plexin, respectively, revealed that olfactory axons in Xenopus tadpoles were classified into several subgroups virtue of expression levels these two molecules. The vomeronasal expressed plexin but not neuropilin. plexin-positive neuropilin-negative form a discrete fiber bundle, even after they joined with principal axons. However, divided at least subclasses;...
We previously demonstrated that differentiated enterocytes from human induced pluripotent stem (iPS) cells exhibited drug-metabolizing activities and cytochrome P450 CYP3A4 inducibility. The aim of this study was to apply iPS cell-derived in pharmacokinetic studies by investigating the characteristics drug transport into enterocyte-like cells. Human cultured on feeder were endodermal using activin A. These endodermal-like then intestinal fibroblast growth factor 2. Finally, epidermal...
Evidence is accumulating that Eph receptor tyrosine kinases and their ligands regulate cell migration axonal guidance during development. It was previously found one of the receptors, EphA4, transiently expressed in subsets chick embryonic motor neurons. Here, expression EphA ephrin‐A subfamily members further examined, dynamic patterns neurons found. EphA3, ephrin‐A2, ephrin‐A5 were also connective tissues limb muscles EphA3 EphA4 expressing innervated muscles, respectively. These...
In Xenopus, ectodermal patterning depends on a mediolateral gradient of BMP signaling, higher in the epidermis and lower neuroectoderm. Neural crest cells are specified at border between neural plate epidermis, intermediate levels signaling. We recently described novel secreted protein, Tsukushi (TSK), which works as antagonist during chick gastrulation. Here, we report Xenopus TSK gene (X-TSK), show that it is involved specification. X-TSK expression accumulates after gastrulation...
BackgroundCell-cell communication is essential in tissue patterning. In early amphibian development, mesoderm formed the blastula-stage embryo through inductive interactions which vegetal cells act on overlying equatorial cells. Members of TGF-β family such as activin B, Vg1, derrière and Xenopus nodal-related proteins (Xnrs) are candidate inducing factors, with further activity to induce endoderm region. TGF-β-like ligands, including BMP, also responsible for patterning germ layers....
The lateral ventricle (LV) is flanked by the subventricular zone (SVZ), a neural stem cell (NSC) niche rich in extrinsic growth factors regulating NSC maintenance, proliferation, and neuronal differentiation. Dysregulation of SVZ causes LV expansion, condition known as hydrocephalus; however, underlying pathological mechanisms are unclear. We show that deficiency proteoglycan Tsukushi (TSK) ependymal cells at surface cerebrospinal fluid results hydrocephalus with neurodevelopmental...
Three classes of signaling molecule, VG1, WNT and BMP, play crucial roles in axis formation the chick embryo. Although VG1 signals have a pivotal function inducing primitive streak Hensen's node embryo midline, their action is complemented by that BMP antagonists protect prospective axial tissue from inhibitory influence BMPs secreted periphery. We previously reported factor, Tsukushi (TSK), expressed node, where it works as antagonist. Here, we describe new for TSK promoting positively...