A5100660554- RNA modifications and cancer
- Circular RNAs in diseases
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- Histone Deacetylase Inhibitors Research
- Gear and Bearing Dynamics Analysis
- DNA Repair Mechanisms
- Alzheimer's disease research and treatments
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Neuropeptides and Animal Physiology
- Genomic variations and chromosomal abnormalities
- Chromosomal and Genetic Variations
- Tribology and Lubrication Engineering
- Monoclonal and Polyclonal Antibodies Research
- Cholinesterase and Neurodegenerative Diseases
- Bone Metabolism and Diseases
- Mechanical Engineering and Vibrations Research
- Connective tissue disorders research
- Neuroinflammation and Neurodegeneration Mechanisms
- Lipid metabolism and biosynthesis
Guangxi Medical University
2024
Guangxi Hydraulic Power Machinery Research Institute
2024
Health Commission of Anhui Province
2024
Fuzhou University
2022-2024
Qilu Hospital of Shandong University
2021-2024
Key Laboratory of Guangdong Province
2023
Chongqing University of Posts and Telecommunications
2023
Peking Union Medical College Hospital
2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2023
Zhejiang Chinese Medical University
2023
The vast majority of G protein-coupled receptors are desensitized by a uniform two-step mechanism: phosphorylation an active receptor followed arrestin binding. arrestin·receptor complex is then internalized. Internalized can be recycled back to the plasma membrane (resensitization) or targeted lysosomes for degradation (down-regulation). intracellular compartment where this choice made and molecular mechanisms involved largely unknown. Here we used two arrestin2 mutants that bind with high...
Abstract N 6 -methyladenosine (m A) modification is an important mechanism in miRNA processing and maturation, but the role of its aberrant regulation human diseases remained unclear. Here, we demonstrate that oncogenic primary microRNA-25 (miR-25) pancreatic duct epithelial cells can be excessively maturated by cigarette smoke condensate (CSC) via enhanced m A mediated NF-κB associated protein (NKAP). This catalyzed overexpressed methyltransferase-like 3 (METTL3) due to hypomethylation...
Breast cancer is one of the most common malignancies and major cause cancer-related death in women. Although importance PIWI-interacting RNAs (piRNAs) has been increasingly recognized, few studies have explored functional mechanism piRNAs breast development progression.We examined top 20 highly expressed based on analysis TCGA data two patient cohorts to test roles cancer. The effects piRNA-36,712 malignant phenotypes chemosensitivity cells were detected vitro vivo. MS2-RIP reporter gene...
DNA damage contributes to brain aging and neurodegenerative diseases. However, the factors stimulating repair stave off functional decline remain obscure. We show that HDAC1 modulates OGG1-initated 8-oxoguanine (8-oxoG) in brain. HDAC1-deficient mice display age-associated accumulation cognitive impairment. stimulates OGG1, a glycosylase known remove 8-oxoG lesions are associated with transcriptional repression. deficiency causes impaired OGG1 activity, at promoters of genes critical for...
Abstract 5-Methylcytosine (m 5 C) is a posttranscriptional RNA modification participating in many critical bioprocesses, but its functions human cancer remain unclear. Here, by detecting the transcriptome-wide m C profiling esophageal squamous cell carcinoma (ESCC), we showed increased methylation ESCC tumors due to overexpressed methyltransferase NSUN2. Aberrant expression of NSUN2 was positively regulated E2F Transcription Factor 1 (E2F1). High levels predicted poor survival patients....
Although PIWI-interacting RNAs (piRNAs) have recently been linked to human diseases, their roles and functions in malignancies remain unclear. This study aimed investigate the significance of some piRNAs colorectal cancer (CRC). Methods: We first analyzed expression profile CRC using TCGA GEO databases. The top 20 highly expressed were selected tested our tumor non-tumor tissue samples. then examined relevance significantly differentially piRNA outcomes 218 patients receiving postoperative...
Osteoporosis is a progressive bone disease characterized by decrease in mass and density, which results an increased risk of fractures. Mesenchymal stem cells (MSCs) are progenitor that can differentiate into osteoblasts, osteocytes adipocytes fat formation. A reduction the differentiation MSCs osteoblasts contributes to impaired formation observed osteoporosis. MicroRNAs (miRNAs) play regulatory role osteogenesis MSC differentiation. MiR-27a has been reported be down-regulated development...
Background: Our previous study has demonstrated an oncogenic role of PIWI-interacting RNA-54265 (piR-54265) in colorectal cancer (CRC). Here, we investigate whether it can be a blood biomarker for population screening and clinical applications. Methods: Serum piR-54265 levels were determined by digital PCR method 209 cancer-free healthy controls, 725 patients with CRC, 1303 other types digestive 192 benign tumors. A prospective case-control analysis was conducted to assess the predictive...
Pseudogenes may play important roles in cancer. Here, we explore the mechanism and function of a pseudogene WTAPP1 progress pancreatic ductal adenocarcinoma (PDAC). RNA was significantly elevated PDAC associated with poor prognosis patients. Overexpression promoted proliferation invasiveness vitro vivo. Mechanistically, N6-methyladenosine (m6A) modification stabilized via CCHC-type zinc finger nucleic-acid binding protein (CNBP), resulting increased levels cells. Excessive bound its...
The tumorigenic mechanism for pancreatic ductal adenocarcinoma (PDAC) is not clear, although chronic inflammation implicated. Here, we identified an inflammatory cytokine–regulated transfer RNA–derived (tRNA-derived) fragment, tRF-21-VBY9PYKHD (tRF-21), as a tumor suppressor in PDAC progression. We found that the biogenesis of tRF-21 could be inhibited by leukemia inhibitory factor and IL-6 via splicing SRSF5. Reduced promoted AKT2/1-mediated heterogeneous nuclear ribonucleoprotein L (hnRNP...
Abstract The molecular mechanism underlying pancreatic ductal adenocarcinoma (PDAC) malignancy remains unclear. Here, we characterize a long intergenic non-coding RNA LINC00842 that plays role in PDAC progression. expression is upregulated and induced by high concentration of glucose via transcription factor YY1. binds to prevents acetylated PGC-1α from deacetylation deacetylase SIRT1 form PGC-1α, an important co-factor regulating cellular metabolism. overexpression causes metabolic switch...
Single nucleotide polymorphisms (SNPs) in the OPRM1 gene have been associated with vulnerability to opioid dependence. The current study identifies an association of intronic SNP (rs9479757) severity heroin addiction among Han-Chinese male addicts. Individual analysis and haplotype-based additional SNPs locus showed that mild was AG genotype, whereas severe GG genotype. In vitro studies such as electrophoretic mobility shift assay, minigene, siRNA, antisense morpholino oligonucleotide...
N6-methyladenosine (m6A) is the most prevalent RNA modification, and effect of its dysregulation on esophageal squamous cell carcinoma (ESCC) development remains unclear. Here, by performing transcriptome-wide m6A sequencing in 16 ESCC tissue samples, we identified key roles TNFRSF1A (also known as TNFR1)-mediated MAPK NF-κB activation ESCC. Mechanistically, a functional protein involved methylation, ATXN2, that augments translation binding to m6A-modified mRNA. Upregulation level, vital...
The new type of nutation reducer with double circular-arc bevel gears as the core is characterized by advantages such high load-bearing capacity, large transmission ratio, and simple structure. existing research focuses on machining methods analysis tooth contact. This article first time establishes an elastohydrodynamic mixed lubrication model for gear. model's accuracy verified comparing it test results. On this basis, combined finite element method contact analysis, meshing...
Genomic instability caused by a deficiency in the DNA damage response and repair has been linked to age-related cognitive decline neurodegenerative diseases. Preventing genomic that ultimately leads neuronal death may provide broadly effective strategy protect against multiple potential genotoxic stressors. Recently, zinc-dependent class I histone deacetylase (HDAC1) identified as critical factor for protecting neurons from deleterious effects of Alzheimer's disease (AD), amyotrophic lateral...
Abstract Although several nucleoside diphosphate (NDP) kinase genes have been cloned in plants, little is known about the functional significance of this enzyme during plant growth and development. We introduced a chimeric gene encoding an antisense RNA NDP under control Arabidopsis heat shock protein HSP81-1 promoter into rice (Oryza sativaL.) plants using Agrobacterium tumefacienstransformation system. The expression down-regulated accumulation mRNA, resulting reduced activity even...
We identified genes based on screening of an Arabidopsis cDNA library for functional suppressors mouse Bax‐induced cell death yeast cells. Interestingly, the encoding AtEBP, known as thaliana ethylene‐responsive element binding protein, was isolated numerous times in screen (82% all suppressors). Full‐length AtEBP and its localization to nucleus were essential suppression death. Morphological abnormality intracellular network that is a hallmark attenuated by expression AtEBP.
N6-methyladenosine (m6A) is the most abundant modification of RNA in eukaryotic cells and play critical roles cancer. While related studies focus on m6A modifications linear RNA, transcriptome-wide profiling exploration circular RNAs cancer still lacking.For detection circRNAs, we developed a new bioinformatics tools called Circm6A applied it to m6A-seq data 77 tissue samples from 58 individuals with pancreatic ductal adenocarcinoma (PDAC).Circm6A performs better than existing circRNA...