A5068564956- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Pancreatic and Hepatic Oncology Research
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Mechanisms of cancer metastasis
- MicroRNA in disease regulation
- Biochemical and Molecular Research
- Ubiquitin and proteasome pathways
- Advanced Breast Cancer Therapies
- Chromosomal and Genetic Variations
- Cancer Genomics and Diagnostics
- ATP Synthase and ATPases Research
- Immune cells in cancer
- RNA and protein synthesis mechanisms
- Cancer Immunotherapy and Biomarkers
- CRISPR and Genetic Engineering
- Cancer Cells and Metastasis
- Phagocytosis and Immune Regulation
- Genomics and Phylogenetic Studies
- Histone Deacetylase Inhibitors Research
- Single-cell and spatial transcriptomics
- HVDC Systems and Fault Protection
Sun Yat-sen University
2018-2025
Sun Yat-sen University Cancer Center
2018-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Peking Union Medical College Hospital
2025
State Key Laboratory of Oncology in South China
2019-2025
Abstract N 6 -methyladenosine (m A) modification is an important mechanism in miRNA processing and maturation, but the role of its aberrant regulation human diseases remained unclear. Here, we demonstrate that oncogenic primary microRNA-25 (miR-25) pancreatic duct epithelial cells can be excessively maturated by cigarette smoke condensate (CSC) via enhanced m A mediated NF-κB associated protein (NKAP). This catalyzed overexpressed methyltransferase-like 3 (METTL3) due to hypomethylation...
Breast cancer is one of the most common malignancies and major cause cancer-related death in women. Although importance PIWI-interacting RNAs (piRNAs) has been increasingly recognized, few studies have explored functional mechanism piRNAs breast development progression.We examined top 20 highly expressed based on analysis TCGA data two patient cohorts to test roles cancer. The effects piRNA-36,712 malignant phenotypes chemosensitivity cells were detected vitro vivo. MS2-RIP reporter gene...
Abstract 5-Methylcytosine (m 5 C) is a posttranscriptional RNA modification participating in many critical bioprocesses, but its functions human cancer remain unclear. Here, by detecting the transcriptome-wide m C profiling esophageal squamous cell carcinoma (ESCC), we showed increased methylation ESCC tumors due to overexpressed methyltransferase NSUN2. Aberrant expression of NSUN2 was positively regulated E2F Transcription Factor 1 (E2F1). High levels predicted poor survival patients....
Although PIWI-interacting RNAs (piRNAs) have recently been linked to human diseases, their roles and functions in malignancies remain unclear. This study aimed investigate the significance of some piRNAs colorectal cancer (CRC). Methods: We first analyzed expression profile CRC using TCGA GEO databases. The top 20 highly expressed were selected tested our tumor non-tumor tissue samples. then examined relevance significantly differentially piRNA outcomes 218 patients receiving postoperative...
Background: Our previous study has demonstrated an oncogenic role of PIWI-interacting RNA-54265 (piR-54265) in colorectal cancer (CRC). Here, we investigate whether it can be a blood biomarker for population screening and clinical applications. Methods: Serum piR-54265 levels were determined by digital PCR method 209 cancer-free healthy controls, 725 patients with CRC, 1303 other types digestive 192 benign tumors. A prospective case-control analysis was conducted to assess the predictive...
Pseudogenes may play important roles in cancer. Here, we explore the mechanism and function of a pseudogene WTAPP1 progress pancreatic ductal adenocarcinoma (PDAC). RNA was significantly elevated PDAC associated with poor prognosis patients. Overexpression promoted proliferation invasiveness vitro vivo. Mechanistically, N6-methyladenosine (m6A) modification stabilized via CCHC-type zinc finger nucleic-acid binding protein (CNBP), resulting increased levels cells. Excessive bound its...
The tumorigenic mechanism for pancreatic ductal adenocarcinoma (PDAC) is not clear, although chronic inflammation implicated. Here, we identified an inflammatory cytokine–regulated transfer RNA–derived (tRNA-derived) fragment, tRF-21-VBY9PYKHD (tRF-21), as a tumor suppressor in PDAC progression. We found that the biogenesis of tRF-21 could be inhibited by leukemia inhibitory factor and IL-6 via splicing SRSF5. Reduced promoted AKT2/1-mediated heterogeneous nuclear ribonucleoprotein L (hnRNP...
Abstract The molecular mechanism underlying pancreatic ductal adenocarcinoma (PDAC) malignancy remains unclear. Here, we characterize a long intergenic non-coding RNA LINC00842 that plays role in PDAC progression. expression is upregulated and induced by high concentration of glucose via transcription factor YY1. binds to prevents acetylated PGC-1α from deacetylation deacetylase SIRT1 form PGC-1α, an important co-factor regulating cellular metabolism. overexpression causes metabolic switch...
Objective Metastasis is the major cause of cancer death. However, what types heterogenous cells in primary tumour and how they metastasise to target organs remain largely undiscovered. Design We performed single-cell RNA sequencing spatial transcriptomic analysis colorectal (CRC) metastases liver (lCRC) or ovary (oCRC). also conducted immunofluorescence staining functional experiments examine mechanism. Results Integrative analyses epithelial reveal a stem-like cell cluster with high protein...
N6-methyladenosine (m6A) is the most prevalent RNA modification, and effect of its dysregulation on esophageal squamous cell carcinoma (ESCC) development remains unclear. Here, by performing transcriptome-wide m6A sequencing in 16 ESCC tissue samples, we identified key roles TNFRSF1A (also known as TNFR1)-mediated MAPK NF-κB activation ESCC. Mechanistically, a functional protein involved methylation, ATXN2, that augments translation binding to m6A-modified mRNA. Upregulation level, vital...
Background The resistance of pancreatic ductal adenocarcinoma (PDAC) to trametinib therapy limits its clinical use. However, the molecular mechanisms underlying in PDAC remain unclear. Objective We aimed illustrate and identify resistance-associated druggable targets, thus improving treatment efficacy trametinib-resistant PDAC. Design established patient-derived xenograft (PDX) models primary cell lines conduct functional experiments. also applied single-cell RNA sequencing, Assay for...
Objective: To investigate the impact of receptor-interacting protein kinase 3 (RIPK3) on major adverse cardiovascular events (MACE) in patients with acute myocardial infarction (AMI) after percutaneous coronary intervention (PCI), as well predictive performance RIPK3 combined traditional risk factors. Methods: This study was a single-center prospective cohort study. It included AMI who underwent PCI at Peking Union Medical College Hospital between September 2017 and November 2017. Baseline...
Abstract N 6 - methyladenosine (m A) modification of gene transcripts plays critical roles in cancer. Here we report transcriptomic m A profiling 98 tissue samples from 65 individuals with pancreatic ductal adenocarcinoma (PDAC). We identify 17,996 peaks 195 hyper-methylated and 93 hypo-methylated PDAC compared adjacent normal tissues. The differential modifications distinguish two subtypes different prognosis outcomes. formation the is driven by a newly identified regulator CSTF2 that...
Abstract N 6-Methyladenosine (m6A) is the most prevalent RNA modification and associated with various biological processes. Proteins that function as readers writers of m6A modifications have been shown to play critical roles in human malignancies. Here, we identified KH-type splicing regulatory protein (KHSRP) an binding contributes progression pancreatic ductal adenocarcinoma (PDAC). High KHSRP levels were detected PDAC predicted poor patient survival. deficiency suppressed growth...
N6-methyladenosine (m6A) is the most abundant modification of RNA in eukaryotic cells and play critical roles cancer. While related studies focus on m6A modifications linear RNA, transcriptome-wide profiling exploration circular RNAs cancer still lacking.For detection circRNAs, we developed a new bioinformatics tools called Circm6A applied it to m6A-seq data 77 tissue samples from 58 individuals with pancreatic ductal adenocarcinoma (PDAC).Circm6A performs better than existing circRNA...
Abstract The therapeutic options for treating pancreatic ductal adenocarcinoma (PDAC) are limited, and resistance to gemcitabine, a cornerstone of PDAC chemotherapy regimens, remains major challenge. N6-methyladenosine (m6A) is prevalent modification in mRNA that has been linked diverse biological processes human diseases. Herein, by characterizing the global m6A profile panel gemcitabine-sensitive gemcitabine-insensitive cells, we identified key role elevated master G0–G1 regulator FZR1...
<p>Proteins identified in RNA pulldown and mass spectrometry analysis with FZR1[m6A] or FZR1[A].</p>
<p>Supplementary Figures 1-6 and supplementary Tables 1-7.</p>
<p>Proteins identified in RNA pulldown and mass spectrometry analysis with FZR1[m6A] or FZR1[A].</p>