A5026596219- Circadian rhythm and melatonin
- Genetics, Aging, and Longevity in Model Organisms
- Dietary Effects on Health
- Epigenetics and DNA Methylation
- Photoreceptor and optogenetics research
- Genetics and Neurodevelopmental Disorders
- Adipose Tissue and Metabolism
- Diet, Metabolism, and Disease
- Lipid metabolism and biosynthesis
- Retinal Development and Disorders
- interferon and immune responses
- Malaria Research and Control
- Immune Response and Inflammation
- Metabolomics and Mass Spectrometry Studies
- Frailty in Older Adults
- Aging and Gerontology Research
- Cytokine Signaling Pathways and Interactions
- Sleep and Wakefulness Research
- Identity, Memory, and Therapy
- RNA modifications and cancer
- Neuroscience and Neuropharmacology Research
- Neurobiology and Insect Physiology Research
The University of Texas Southwestern Medical Center
2017-2024
The University of Texas at Dallas
2023
Southwestern Medical Center
2021
University of Rochester
2021
Howard Hughes Medical Institute
2017
Research Centre in Biological Chemistry of Córdoba
2010-2014
Consejo Nacional de Investigaciones Científicas y Técnicas
2010-2014
Universidad Nacional de Córdoba
2010-2014
Caloric restriction (CR) prolongs life span, yet the mechanisms by which it does so remain poorly understood. Under CR, mice self-impose chronic cycles of 2-hour feeding and 22-hour fasting, raising question if is calories, or time day that cause this increased span. We show here 30% CR was sufficient to extend span 10%; however, a daily fasting interval circadian alignment acted together 35% in male C57BL/6J mice. These effects were independent body weight. Aging induced widespread...
Abstract Aging, often considered a result of random cellular damage, can be accurately estimated using DNA methylation profiles, the foundation pan-tissue epigenetic clocks. Here, we demonstrate development universal pan-mammalian clocks, 11,754 arrays from our Mammalian Methylation Consortium, which encompass 59 tissue types across 185 mammalian species. These predictive models estimate age with high accuracy ( r > 0.96). Age deviations correlate human mortality risk, mouse somatotropic...
Using DNA methylation profiles (
ABSTRACT Aging is often perceived as a degenerative process resulting from random accrual of cellular damage over time. Despite this, age can be accurately estimated by epigenetic clocks based on DNA methylation profiles almost any tissue the body. Since such pan-tissue have been successfully developed for several different species, we hypothesized that one build pan-mammalian measure in all mammalian species. To address generated data using 11,754 arrays, each profiling up to 36 thousand...
Malarial rhythmic fevers are the consequence of synchronous bursting red blood cells (RBCs) on completion malaria parasite asexual cell cycle. Here, we hypothesized that an intrinsic clock in
The vertebrate retina is known to contain three classes of photoreceptor cells: cones and rods responsible for vision, intrinsically photoresponsive retinal ganglion cells (RGCs) involved in diverse non-visual functions such as photic entrainment daily rhythms pupillary light responses. In this paper we investigated the potential intrinsic photoresponsiveness rat RGC line, RGC-5, by testing presence visual opsins assessing expression immediate-early gene protein c-Fos changes intracellular...
Abstract Animal behavior emerges from integration of many processes with different spatial and temporal scales. Dynamical behavioral patterns, including daily ultradian rhythms the dynamical microstructure (i.e., autocorrelations properties), can be differentially affected by external cues. Identifying these patterns is important for understanding how organisms adapt to their environment, yet unbiased methods quantify changes over multiple scales are lacking. Herein, we combine a wavelet...
Transcriptional regulation of numerous interferon-regulated genes, including Toll-like receptor 3 (Tlr3), which encodes an innate immune sensor viral double-stranded RNA, depends on the interferon regulatory factor 1 (IRF1) and IRF2 transcription factors. We detected specific abrogation macrophage responses to polyinosinic-polycytidylic acid (poly(I:C)) resulting from three independent N-ethyl-N-nitrosourea-induced mutations in host cell C2 (Hcfc2). Hcfc2 compromised survival during...
Circadian clocks regulate the temporal organization of several biochemical processes, including lipid metabolism, and their disruption leads to severe metabolic disorders. Immortalized cell lines acting as circadian display daily variations in [32P]phospholipid labeling; however, regulation glycerophospholipid (GPL) synthesis by internal remains unknown. Here we found that arrested NIH 3T3 cells synchronized with a 2 h-serum shock exhibited oscillations a) labeling total [3H] GPLs, lowest...
The circadian system involves central and peripheral oscillators regulating temporally biochemical processes including lipid metabolism; their disruption leads to severe metabolic diseases (obesity, diabetes, etc). Here, we investigated the temporal regulation of glycerophospholipid (GPL) synthesis in mouse liver, a well-known oscillator. Mice were synchronized 12:12 h light–dark (LD) cycle then released constant darkness with food ad libitum. Livers collected at different times exhibited...
Extended consumption of food into the rest period perturbs phase relationship between circadian clocks in periphery and brain has deleterious effects on health through mechanisms that remain incompletely understood. Beyond liver, how other metabolic organs respond to hypocaloric diet (amount timing) is largely unexplored. We investigated feeding time impacts gene expression white (eWAT) brown (BAT) adipose tissues comparison liver hypothalamus. With automated feeders, we restricted either...
Abstract Caloric restriction (CR) promotes longevity in several species. Classic CR protocols often lead to chronic cycles of 2h-feeding/22h-fasting, raising the question whether calories, fasting or time day are causal. To address this, we tested an AL control group and five with different timing duration feeding/fasting cycles. C57BL/6J male mice were subjected 30% as one single meal a at beginning night (classical < 2h feeding, CR-day CR-night), smaller meals distributed for 12h...