A5002760106- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Pharmacological Effects of Natural Compounds
- Circular RNAs in diseases
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Chronic Lymphocytic Leukemia Research
- Respiratory viral infections research
- Immune Response and Inflammation
- HER2/EGFR in Cancer Research
- Distributed and Parallel Computing Systems
- Animal Disease Management and Epidemiology
- Curcumin's Biomedical Applications
- Phagocytosis and Immune Regulation
- FinTech, Crowdfunding, Digital Finance
- Cell Adhesion Molecules Research
- Blockchain Technology Applications and Security
- Phytochemical compounds biological activities
- COVID-19 Clinical Research Studies
Bioscience (China)
2020-2024
University of Electronic Science and Technology of China
2016-2024
The University of Texas Health Science Center at Houston
2017-2023
Brown Foundation
2017-2023
Bioscience Research
2021
Xiamen University
2010-2018
Institute of Molecular Medicine
2017
Southwest Jiaotong University
2008
United States Department of the Army
1991
Journal Article Prospective, Double-Blind, Concurrent, Placebo-Controlled Clinical Trial of Intravenous Ribavirin Therapy Hemorrhagic Fever with Renal Syndrome Get access John W. Huggins, Huggins Search for other works by this author on: Oxford Academic PubMed Google Scholar Chin Min Hsiang, Hsiang Thomas M. Cosgriff, Cosgriff Mei Ying Guang, Guang Joseph I. Smith, Smith Zhen Ou Wu, Wu James LeDuc, LeDuc Zhi Ming Zheng, Zheng Meegan, Meegan Qi Nan Wang, Wang ... Show more Dwayne D. Oland,...
Valine-citrulline linkers are commonly used as enzymatically cleavable for antibody-drug conjugates. While stable in human plasma, these unstable mouse plasma due to susceptibility an extracellular carboxylesterase. This instability often triggers premature release of drugs circulation, presenting a molecular design challenge. Here, we report that conjugate with glutamic acid-valine-citrulline is responsive enzymatic drug but undergoes almost no cleavage mice. We demonstrate this construct...
To effectively improve treatment for acute myeloid leukemia (AML), new molecular targets and therapeutic approaches need to be identified. Chimeric antigen receptor (CAR)-modified T cells targeting tumor-associated antigens have shown promise in the of some malignancies. However, CAR-T cell development AML has been limited by lack an with high specificity that is not present on normal hematopoietic stem cells, thus will result myelotoxicity. Here we demonstrate leukocyte immunoglobulin-like...
Abstract Efficacious interventions are urgently needed for the treatment of COVID-19. Here, we report a monoclonal antibody (mAb), MW05, with SARS-CoV-2 neutralizing activity by disrupting interaction receptor binding domain (RBD) angiotensin-converting enzyme 2 (ACE2) receptor. Crosslinking Fc FcγRIIB mediates antibody-dependent enhancement (ADE) MW05. This is eliminated introducing LALA mutation to region (MW05/LALA). Potent prophylactic and therapeutic effects against observed in rhesus...
Abstract Understanding the underlying molecular mechanisms behind ADE of SARS-CoV-2 is critical for development safe and effective therapies. Here, we report that two neutralizing mAbs, MW01 MW05, could enhance infection pseudovirus on FcγRIIB-expressing B cells. X-ray crystal structure determination S trimer-binding modeling showed MW05 bind to RBDs in trimer with both “up” “down” states. While, mAb MW07, which has no activity only binds RBD state. Monovalent completely diminished compared...
Antibody-drug conjugates (ADCs) are emerging therapeutic agents in the treatment of cancer, and various conjugation strategies chemical linkers have been developed to efficiently construct ADCs. Despite previous extensive efforts for improving efficiency ADC homogeneity, most date load only single payloads. Branched that can multiple payload molecules yet be fully explored. It is logical envisage a multi-loading strategy allows increase drug-to-antibody ratio (DAR) with less or enzymatic...
As synchrophasor data start to play a significant role in power system operation and dynamic study, processing analysis capability are critical wide-area measurement systems (WAMSs). The frequency monitoring network (FNET/GridEye) is WAMS that collects from hundreds of disturbance recorders at the distribution level. previous FNET/GridEye center limited by its storage computation power. Targeting scalability, extensibility, concurrency, robustness, distributed analytics platform proposed...
Abstract Therapeutic strategies are urgently needed for patients with acute myeloid leukemia (AML). Leukocyte immunoglobulin-like receptor B4 (LILRB4), which suppresses T-cell activation and supports tissue infiltration of AML cells, represents an attractive drug target anti-AML therapeutics. Here, we report the identification development LILRB4-specific humanized mAb that blocks LILRB4 activation. This mAb, h128-3, showed potent activity in blocking monocytic various models including...
Background Current immune checkpoint blockade strategies have been successful in treating certain types of solid cancer. However, monotherapies not against most hematological malignancies including multiple myeloma and leukemia. There is an urgent need to identify new targets for development cancer immunotherapy. LILRB1, immunoreceptor tyrosine-based inhibitory motif-containing receptor, widely expressed on human cells, B monocytes macrophages, dendritic cells subsets natural killer (NK) T...
Acute myeloid leukemia (AML) is the most common and aggressive blood cancer in adults. In particular, significant unmet medical needs exist for effective treatment strategies acute myelomonocytic (M4) monocytic (M5) AML subtypes. Antibody-drug conjugates (ADC) are a promising drug class therapy, as demonstrated by FDA-approved anti-CD33 ADC, gemtuzumab ozogamicin (Mylotarg). However, CD33 expressed normal hematopoietic stem cells, highlighting critical need to identify AML-specific targets...
Background: Even though PD-1/PD-L1 is an identified key "don't find me" signal to active adaptive immune system for cancer treatment, the overall response rate (ORR) all patients still limited.Other effective therapeutic modalities bridge innate and immunity improve ORR are urgently needed.Recently, CD47/SIRPα interaction confirmed as a critical eat immunity.However, red blood cell (RBC) toxicity big concern development of CD47-based anti-cancer therapeutics.Methods: Here, we report...
Abstract Background Targeting the TGF-β pathway in tumor therapy has proven challenging due to highly context-dependent functions of TGF-β. Integrin αvβ8, a pivotal activator TGF-β, been implicated signaling within tumors, as demonstrated by significant anti-tumor effects anti-αvβ8 antibodies. Nevertheless, expression profile αvβ8 remains subject debate, and precise mechanisms underlying antibodies are not yet fully elucidated. Methods We utilized single-cell RNA sequencing assess across...
Hepatic platelet accumulation contributes to acetaminophen (APAP)-induced liver injury (AILI). However, little is known about the molecular pathways involved in recruitment and whether targeting such could attenuate AILI. Mice were fasted overnight before intraperitoneally (i.p.) injected with APAP at a dose of 210 mg/kg for male mice 325 female mice. Platelets adherent Kupffer cells determined both patients overdosed APAP. The impact α-chitinase 3-like-1 (α-Chi3l1) on alleviation AILI was...
The current immune checkpoint blockade therapy has been successful in treating some cancers but not others. New molecular targets and therapeutic approaches of cancer immunology need to be identified. Leukocyte associated immunoglobulin like receptor 1 (LAIR1) is an inhibitory expressing on most cell types. However, it remains a question whether we can specifically actively block LAIR1 signaling activate responses for treatment. Here report the development specific antagonistic anti-LAIR1...
The global pandemic of COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in widespread social and economic disruption. Effective interventions are urgently needed for the prevention treatment COVID-19. Neutralizing monoclonal antibodies (mAbs) have demonstrated their prophylactic therapeutic efficacy against SARS-CoV-2, several been granted authorization emergency use. Here, we discover characterize a fully human cross-reactive mAb, MW06, which...
Abstract The existing T cell–centered immune checkpoint blockade therapies have been successful in treating some but not all patients with cancer. Immunosuppressive myeloid cells, including myeloid-derived suppressor cells (MDSC), that inhibit antitumor immunity and support multiple steps of tumor development are recognized as one the major obstacles cancer treatment. Leukocyte Ig-like receptor subfamily B3 (LILRB3), an inhibitory containing tyrosine-based motifs (ITIM), is expressed solely...
Anti-CTLA-4 antibodies faced challenges due to frequent adverse events and limited efficacy, which spurred the exploration of next-generation CTLA-4 therapeutics balance regulatory T cells (Tregs) depletion CD8 activation. CCR8, identified primarily on tumor-infiltrating Tregs, has become a target interest anti-tumor effects demonstrated by CCR8 antibody-mediated Tregs depletion. Single-cell RNA sequencing analysis reveals that CCR8-positive constitute small subset, with concurrent...
Human cytomegalovirus (HCMV) can cause life-threatening infection in immunosuppressed patients, and utero that may lead to birth defects. No vaccine is currently available. HCMV healthy subjects generally asymptomatic, virus persists as latent for life. Host immunity effective against reactivation super-infection with another strain. Thus, candidates able elicit immune responses similar those of natural confer protection. Since neutralization essential prophylactic vaccines, it important...
The host immune response to human cytomegalovirus (HCMV) is effective against HCMV reactivation from latency, though not sufficient clear the virus. T cells are primarily responsible for control of viral reactivation. When system compromised, as in transplant recipients with immunosuppression, and progressive infection can cause serious morbidity mortality. Adoptive cell therapy recipients. However, it a highly personalized therapeutic regimen difficult implement routine clinical practice....
Rapid and sensitive diagnosis of influenza is urgently needed to address the limitations low sensitivity associated with current rapid tests available for clinics on‐site monitoring. A novel horseradish peroxidase (HRP)‐labeled lateral flow immunoassay strip (HRP‐LFIA) simultaneous detection differentiation (INF A) B B) viruses were developed. This was based on signal amplification by HRP‐catalyzed oxidation 3, 3′, 5, 5′‐tetramethylbenzidine forming a colored insoluble product, which...
ABSTRACT Influenza virus still poses a major threat to human health worldwide. The nucleoprotein (NP) of influenza A plays an essential role in the viral replication and transcription hence becomes promising therapeutic target. NP forms complicated conformation under native conditions might denature when performing immunoassays such as western blot study function. Therefore, it is useful make specific monoclonal antibody (mAb) that recognizes linear epitope instead conformational epitope. In...