A5091422877- Innovative Microfluidic and Catalytic Techniques Innovation
- Genetic and phenotypic traits in livestock
- Microfluidic and Capillary Electrophoresis Applications
- Animal Genetics and Reproduction
- Genetic Mapping and Diversity in Plants and Animals
- Genomics and Phylogenetic Studies
- Gene expression and cancer classification
- Cancer-related molecular mechanisms research
- Ultrasound and Hyperthermia Applications
- Electrowetting and Microfluidic Technologies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Electrohydrodynamics and Fluid Dynamics
- Nanofabrication and Lithography Techniques
- Meteorological Phenomena and Simulations
- Plant Molecular Biology Research
- Advances in Cucurbitaceae Research
- Optical Coatings and Gratings
- Single-cell and spatial transcriptomics
- Photoacoustic and Ultrasonic Imaging
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Cancer Genomics and Diagnostics
- CRISPR and Genetic Engineering
- Pancreatic and Hepatic Oncology Research
- Advanced Cellulose Research Studies
- Ultrasound Imaging and Elastography
Seoul National University
2015-2024
Korea University
2020-2024
Sejong University
2023
Korea Advanced Institute of Science and Technology
2022
Yonsei University
2018-2021
Incheon St. Mary's Hospital
2019-2020
Brigham and Women's Hospital
2017-2019
Harvard University
2017-2019
Kyung Hee University
2019
University of Toronto
2005-2018
Small-scale engineering: A new strategy for the production of monodisperse colloid particles with precise control their size, shape, and composition has been developed. Highly liquid droplets are formed by using a microfluidic device. The shaped in microchannel (spheres, disks, rods; see images) solidified situ either polymerization or thermal setting.
This paper reports a microfluidic method for fast continuous synthesis of Janus particles and three-phase with narrow size distribution. Synthesis included emulsification monomer liquids in-situ photoinitiated polymerization multiphase droplets. We show the strategy precise control over structure their structure-dependent assembly in clusters. demonstrate an asymmetric chemical modification surface JPs by conjugating them protein molecules. The ternary were synthesized from largely...
We report a novel approach to continuous and scalable production of core−shell droplets polymer capsules in microfluidic devices. The described method is also useful the synthesis particles with nonspherical shapes. used capillary instability-driven break-up liquid jet formed by two immiscible fluids. Precise control emulsification each allowed for highly monodisperse predetermined diameter cores thickness shells. achieved over number per droplet location droplet. carried out fast throughput...
The history of African indigenous cattle and their adaptation to environmental human selection pressure is at the root remarkable diversity. Characterization this diversity an essential step towards understanding genomic basis productivity survival under farming systems. We analyze patterns genetic variation by sequencing 48 genomes from five populations comparing them 53 commercial taurine breeds. find highest among zebu sanga cattle. Our search for regions reveals signatures adaptive...
This article provides an overview of our work in the area synthesis polymer particles continuous microfluidic reactors. The method includes (a) generation highly monodisperse monomer droplets a flow-focusing device and (b) in-situ solidification these by means photopolymerization. We discuss effect properties on emulsification process, polymerization rate production high-quality particles, role material droplet formation, with different shapes compositions. also demonstrate ordered arrays...
This article describes emulsification in a microfluidic double droplet generator (DDR) comprising two consecutive flow-focusing devices with locally modified surface chemistry. We generated W/O/W, O/O/W and O/W/O emulsions precisely controlled sizes morphology of droplets. Secondly, by combining mechanisms formation (the mechanism the break up liquid threads at T-junction) we produced multiple populations droplets varying size and/or composition. These were used as structural units for...
Miniaturisierte Technik: Eine neue Strategie für die größen-, form- und zusammensetzungsspezifische Herstellung monodisperser Kolloidpartikel wird vorgestellt. Hoch monodisperse Flüssigkeitstropfen werden mithilfe einer Mikrofluidikvorrichtung gebildet. Die Tropfen in einem Mikrokanal Form gebracht (Kugeln, Scheiben Stäbe; siehe Bilder) situ entweder durch Polymerisation oder Thermofixierung verfestigt.
Journal Article High-throughput combinatorial cell co-culture using microfluidics Get access Ethan Tumarkin, Tumarkin Department of Chemistry, University Toronto, 80 Saint George Street, Ontario, M5S 3H6, Canada Search for other works by this author on: Oxford Academic Google Scholar Lsan Tzadu, Tzadu Elizabeth Csaszar, Csaszar Institute Biomaterials & Biomedical Engineering, 164 College 3G9, CanadaDepartment Chemical Engineering and Applied 200 3E5, Minseok Seo, Seo Hong Zhang, Zhang Anna...
Background: Abalones are large marine snails in the family Haliotidae and genus Haliotis belonging to class Gastropoda of phylum Mollusca. The contains only one genus, Haliotis, this single is known contain several species abalone. With 18 additional subspecies, most comprehensive treatment considers 56 valid [1]. Abalone an economically important fishery aquaculture animal that considered a highly prized seafood delicacy. total global supply abalone has increased 5-fold since 1970s farm...
This paper describes geometric coupling of the dynamics break-up liquid threads in parallel flow-focusing devices (FFD), which are integrated into a multiple quadruple-microfluidic droplet generator (QDG). We show weak parametric between FFDs with an identical design, leads to slight broadening distribution sizes droplets. Using distinct geometries we simultaneously generated several populations droplets different volumes, yet, each these was characterized by narrow size distribution....
New medical imaging contrast agents that permit multiple and therapy applications using a single agent can result in more accurate diagnosis local treatment of diseased tissue. Solid nanoparticles (NPs) (5−150 nm size) have emerged as promising agents, micrometer-scale, perfluorocarbon gas-filled microbubbles (MBs) used patients intravascular ultrasound agents. We propose the modular combination small, solid NPs larger, highly compressible MBs into is an effective way to attain desired...
Abstract We propose eight data transformations ( r , r2 rv rv2 l l2 lv and lv2 ) for RNA-seq analysis aiming to make the transformed sample mean be representative of distribution center since it is not always possible transform count satisfy normality assumption. Simulation studies showed that sets with small (e.g., nCases = nControls 3) or large size 100) limma based on from performed better than voom transformation in term accuracy, FDR, FNR. For datasets moderate 30 50), similarly...
Multiple gene expression studies have been performed separately in peripheral blood, lung, and airway tissues to study COPD. We RNA-sequencing profiling of large-airway epithelium, alveolar macrophage blood samples from the same subset COPD cases controls COPDGene who underwent bronchoscopy at a single center. Using statistical set enrichment approaches, we sought improve understanding by studying sets pathways across these tissues, beyond individual genomic determinants. differential...
Abstract High dimensionality and noise have limited the new biological insights that can be discovered in scRNA-seq data. While reduction tools been developed to extract signals from data, they often require manual determination of signal dimension, introducing user bias. Furthermore, a common data preprocessing method, log normalization, unintentionally distort Here, we develop scLENS, tool circumvents long-standing issues distortion input. Specifically, identify primary cause during...
We have measured normal and lateral interactions across a range of different liquids between mica surfaces using surface force balance (SFB). The were prepared either by melt cutting Pt wire standard procedures in our laboratories or tearing sheets (that had not been exposed to Pt) off from freshly cleaved sheet mica. AFM micrographs revealed the substantial absence nanoparticles on cut torn-off surfaces. Normal-force versus surface-separation (D) profiles shear D measurements for purified...
We report the results of a systematic study effect surface energy walls microchannels on emulsification in parallel flow-focusing microfluidic devices. investigated formation water-in-oil (W/O) and oil-in-water (O/W) emulsions found that stability depends critically preferential wetting device by continuous phase. The condition for stable operation is, however, different than complete phase at equilibrium. W/O form when advancing contact angle water channel wall exceeds theta approximately...
Droplets of liquid perfluorocarbon (PFC) are under study as the next generation contrast agents for ultrasound (US). These droplets can be selectively vaporized into echogenic gas bubbles in situ by externally applied US, with numerous applications to diagnosis and therapy. However, little is known about mechanisms droplet vaporization stability so produced. Here we observe optically fluorescent PFC newly created bubbles. Fluorescent markers were used label either core or shell droplets. It...
Results of recent studies on gut microbiota have suggested that obesogenic bacteria exacerbate obesity and metabolic dysfunction in the host when fed a high fat diet (HFD). In order to explore obesity-associated bacterial candidates their response diet, composition faecal communities was investigated by analyzing 16S rRNA gene sequences mice. Dietary intervention with probiotics Garcinia cambogia extract attenuated weight gain adipocyte size HFD-fed To identify obesity-causative microbiota,...
Abstract Many recent RNA-seq studies were focused mainly on detecting the differentially expressed genes (DEGs) between two or more conditions. In contrast, only a few attempts have been made to detect associated with quantitative traits, such as obesity index and milk yield, experiment large number of biological replicates. This study illustrates linear model application trait (TAGs) detection in real datasets: 89 replicated human related data 21 Holsteins’ production data. Based these...
We used coupling of flow and geometric confinement to assemble emulsion droplets in two-dimensional gliding lattices with a high degree order symmetry. Highly monodisperse discoid circular shapes were generated microfluidic flow-focusing device. Originally, close-packed formed from these droplets. Progressive led the gradual deformation disks: first, they elongated direction parallel then transformed into hexagons. Assembly driven by combination also allowed for formation bimodal size...
ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTContinuous Synthesis of Copolymer Particles in Microfluidic ReactorsPatrick C. Lewis, Robert R. Graham, Zhihong Nie, Shengqing Xu, Minseok Seo, and Eugenia KumachevaView Author Information Department Chemistry, University Toronto, 80 Saint George Street, Ontario M5S 3H6, Canada Cite this: Macromolecules 2005, 38, 10, 4536–4538Publication Date (Web):April 15, 2005Publication History Received17 January 2005Revised11 March 2005Published online15 April...
There has been recent interest in developing new, targeted, perfluorocarbon (PFC) droplet-based contrast agents for medical imaging (e.g., magnetic resonance imaging, X-ray/computed tomography, and ultrasound imaging). However, due to the large number of potential PFCs droplet stabilization strategies available, it is challenging determine advance PFC formulation that will result optimal vivo behavior performance required clinical success. We propose integration fluorescent quantum dots...